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Literature summary for 2.1.1.263 extracted from

  • Niehaus, T.D.; Kinison, S.; Okada, S.; Yeo, Y.S.; Bell, S.A.; Cui, P.; Devarenne, T.P.; Chappell, J.
    Functional identification of triterpene methyltransferases from Botryococcus braunii race B (2012), J. Biol. Chem., 287, 8163-8173.
    View publication on PubMed
Search for more literature for 2.1.1.263

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of TMT-3 in Saccharomyces cerevisiae microsomes leading to mono- and dimethylated products, co-expression of various TMT and SMT-like genes in Saccharomyces cerevisiae strain TN-7 Botryococcus braunii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten enzyme kinetics for TMT-3 Botryococcus braunii

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Botryococcus braunii 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 S-adenosyl-L-methionine + C30 botryococcene Botryococcus braunii overall reaction 2 S-adenosyl-L-homocysteine + 3,20-dimethyl-1,2,21,22-tetradehydro-2,3,20,21-tetrahydrobotryococcene
-
 ir
2 S-adenosyl-L-methionine + C30 botryococcene Botryococcus braunii race B overall reaction 2 S-adenosyl-L-homocysteine + 3,20-dimethyl-1,2,21,22-tetradehydro-2,3,20,21-tetrahydrobotryococcene
-
 ir
S-adenosyl-L-methionine + 20-methyl-21,22-didehydro-20,21-dihydrobotryococcene Botryococcus braunii
-
 S-adenosyl-L-homocysteine + 3,20-dimethyl-1,2,21,22-tetradehydro-2,3,20,21-tetrahydrobotryococcene
-
 ?
S-adenosyl-L-methionine + 3-methyl-1,2-didehydro-2,3-dihydrobotryococcene Botryococcus braunii
-
 S-adenosyl-L-homocysteine + 3,20-dimethyl-1,2,21,22-tetradehydro-2,3,20,21-tetrahydrobotryococcene
-
 ?
S-adenosyl-L-methionine + C30 botryococcene Botryococcus braunii
-
 S-adenosyl-L-homocysteine + 20-methyl-21,22-didehydro-20,21-dihydrobotryococcene
-
 ?
S-adenosyl-L-methionine + C30 botryococcene Botryococcus braunii
-
 S-adenosyl-L-homocysteine + 3-methyl-1,2-didehydro-2,3-dihydrobotryococcene
-
 ?
S-adenosyl-L-methionine + C30 botryococcene Botryococcus braunii race B
-
 S-adenosyl-L-homocysteine + 3-methyl-1,2-didehydro-2,3-dihydrobotryococcene
-
 ?

Organism

Organism UniProt Comment Textmining
Botryococcus braunii
-
-
-
Botryococcus braunii race B
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.25
-
 recombinant enzyme, pH 7.5, 37°C Botryococcus braunii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 S-adenosyl-L-methionine + C30 botryococcene overall reaction Botryococcus braunii 2 S-adenosyl-L-homocysteine + 3,20-dimethyl-1,2,21,22-tetradehydro-2,3,20,21-tetrahydrobotryococcene
-
 ir
2 S-adenosyl-L-methionine + C30 botryococcene overall reaction Botryococcus braunii race B 2 S-adenosyl-L-homocysteine + 3,20-dimethyl-1,2,21,22-tetradehydro-2,3,20,21-tetrahydrobotryococcene
-
 ir
additional information mono- and dimethylated products with terminal carbons C-22/C-20 as the atomic acceptor sites for the methyl additions to botryococcene, NMR analysis, overview. TMT-3 shows only low activity with squalene Botryococcus braunii ?
-
 ?
S-adenosyl-L-methionine + 20-methyl-21,22-didehydro-20,21-dihydrobotryococcene
-
 Botryococcus braunii S-adenosyl-L-homocysteine + 3,20-dimethyl-1,2,21,22-tetradehydro-2,3,20,21-tetrahydrobotryococcene
-
 ?
S-adenosyl-L-methionine + 3-methyl-1,2-didehydro-2,3-dihydrobotryococcene
-
 Botryococcus braunii S-adenosyl-L-homocysteine + 3,20-dimethyl-1,2,21,22-tetradehydro-2,3,20,21-tetrahydrobotryococcene
-
 ?
S-adenosyl-L-methionine + C30 botryococcene
-
 Botryococcus braunii S-adenosyl-L-homocysteine + 20-methyl-21,22-didehydro-20,21-dihydrobotryococcene
-
 ?
S-adenosyl-L-methionine + C30 botryococcene
-
 Botryococcus braunii S-adenosyl-L-homocysteine + 3-methyl-1,2-didehydro-2,3-dihydrobotryococcene
-
 ?
S-adenosyl-L-methionine + C30 botryococcene
-
 Botryococcus braunii race B S-adenosyl-L-homocysteine + 3-methyl-1,2-didehydro-2,3-dihydrobotryococcene
-
 ?

Synonyms

Synonyms Comment Organism
TMT-3
-
 Botryococcus braunii
triterpene methyltransferase-3
-
 Botryococcus braunii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Botryococcus braunii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Botryococcus braunii

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
 Botryococcus braunii

General Information

General Information Comment Organism
metabolism TMT-3 shows a striking preference for botryococcene as its methyl acceptor substrate Botryococcus braunii