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Information on EC 2.1.1.193 - 16S rRNA (uracil1498-N3)-methyltransferase and Organism(s) Mycobacterium tuberculosis and UniProt Accession P9WGX1

for references in articles please use BRENDA:EC2.1.1.193
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IUBMB Comments
The enzyme specifically methylates uracil1498 at N3 in 16S rRNA.
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This record set is specific for:
Mycobacterium tuberculosis
UNIPROT: P9WGX1
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The taxonomic range for the selected organisms is: Mycobacterium tuberculosis
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Reaction Schemes
Synonyms
rv2372c, duf558 protein, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:16S rRNA (uracil1498-N3)-methyltransferase
The enzyme specifically methylates uracil1498 at N3 in 16S rRNA.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + uracil1498 in 16S rRNA
S-adenosyl-L-homocysteine + N3-methyluracil1498 in 16S rRNA
show the reaction diagram
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + uracil1498 in 16S rRNA
S-adenosyl-L-homocysteine + N3-methyluracil1498 in 16S rRNA
show the reaction diagram
-
-
-
?
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
x-ray crystallography
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
sitting drop vapor diffusion method, using 100 mM Tris-HCl pH 8.0, 1.5 M potassium acetate, at 24°C
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Ni-NTA column chromatography and Mono S column chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21 (DE3) and RsmE-deleted Escherichia coli KL16 cells
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Kumar, A.; Kumar, S.; Taneja, B.
The structure of Rv2372c identifies an RsmE-like methyltransferase from Mycobacterium tuberculosis
Acta Crystallogr. Sect. D
70
821-832
2014
Mycobacterium tuberculosis (P9WGX1), Mycobacterium tuberculosis
Manually annotated by BRENDA team