Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 (DE3) and RsmE-deleted Escherichia coli KL16 cells | Mycobacterium tuberculosis |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 100 mM Tris-HCl pH 8.0, 1.5 M potassium acetate, at 24°C | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the deletion of critical residues at the C-terminus of Rv2372c leads to an inability of the protein to form stable dimers and to abolition of the methyltransferase activity | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + uracil1498 in 16S rRNA | Mycobacterium tuberculosis | - |
S-adenosyl-L-homocysteine + N3-methyluracil1498 in 16S rRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WGX1 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and Mono S column chromatography | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + uracil1498 in 16S rRNA | - |
Mycobacterium tuberculosis | S-adenosyl-L-homocysteine + N3-methyluracil1498 in 16S rRNA | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | x-ray crystallography | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
RsmE | - |
Mycobacterium tuberculosis |
Rv2372c | - |
Mycobacterium tuberculosis |