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Information on EC 2.1.1.189 - 23S rRNA (uracil747-C5)-methyltransferase and Organism(s) Escherichia coli and UniProt Accession P75817

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EC Tree
     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.189 23S rRNA (uracil747-C5)-methyltransferase
IUBMB Comments
The enzyme specifically methylates uracil747 at C5 in 23S rRNA.
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This record set is specific for:
Escherichia coli
UNIPROT: P75817
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Word Map
The taxonomic range for the selected organisms is: Escherichia coli
The expected taxonomic range for this enzyme is: Bacteria, Archaea
Reaction Schemes
Synonyms
rlmcd, pab0760, sp_1029, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
RNA uridine methyltransferase B
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m5U methyltransferase
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-
RumB
-
formerly
SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:23S rRNA (uracil747-C5)-methyltransferase
The enzyme specifically methylates uracil747 at C5 in 23S rRNA.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + uracil747 in 23S rRNA
S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA
show the reaction diagram
it is not possible to generate a recombinant version of YbjF that retains in vitro activity, so the function of this enzyme is defined in vivo by engineering a ybjF knockout strain
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-
?
S-adenosyl-L-methionine + uracil747 in 23S rRNA
S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + uracil747 in 23S rRNA
S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA
show the reaction diagram
-
-
-
-
?
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
comparison of the methylation patterns in 23S rRNAs from YbjF+ and YbjF- strains shows that the latter differs only in the lack of the 5-methyluracil747 modification
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression of a intein-fusion protein with an N-terminal His-tag
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Madsen, C.T.; Mengel-Jorgensen, J.; Kirpekar, F.; Douthwaite, S.
Identifying the methyltransferases for m(5)U747 and m(5)U1939 in 23S rRNA using MALDI mass spectrometry
Nucleic Acids Res.
31
4738-4746
2003
Escherichia coli (P75817)
Manually annotated by BRENDA team
Auxilien, S.; Rasmussen, A.; Rose, S.; Brochier-Armanet, C.; Husson, C.; Fourmy, D.; Grosjean, H.; Douthwaite, S.
Specificity shifts in the rRNA and tRNA nucleotide targets of archaeal and bacterial m5U methyltransferases
RNA
17
45-53
2011
Escherichia coli, Pyrococcus abyssi
Manually annotated by BRENDA team