Cloned (Comment) | Organism |
---|---|
expression of a intein-fusion protein with an N-terminal His-tag | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P75817 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + uracil747 in 23S rRNA | it is not possible to generate a recombinant version of YbjF that retains in vitro activity, so the function of this enzyme is defined in vivo by engineering a ybjF knockout strain | Escherichia coli | S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
RNA uridine methyltransferase B | - |
Escherichia coli |
RumB | - |
Escherichia coli |
YbjF | - |
Escherichia coli |
General Information | Comment | Organism |
---|---|---|
malfunction | comparison of the methylation patterns in 23S rRNAs from YbjF+ and YbjF- strains shows that the latter differs only in the lack of the 5-methyluracil747 modification | Escherichia coli |