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Information on EC 2.1.1.10 - homocysteine S-methyltransferase and Organism(s) Rattus norvegicus and UniProt Accession O09171

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EC Tree
     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.10 homocysteine S-methyltransferase
IUBMB Comments
The enzyme uses S-adenosyl-L-methionine as methyl donor less actively than S-methyl-L-methionine.
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This record set is specific for:
Rattus norvegicus
UNIPROT: O09171
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The taxonomic range for the selected organisms is: Rattus norvegicus
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
homocysteine methyltransferase, homocysteine s-methyltransferase, athmt-1, homocysteine transmethylase, l-homocysteine s-methyltransferase, yagd protein, s-methylmethionine homocysteine methyltransferase, athmt-2, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
adenosylmethionine transmethylase
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adenosylmethionine:homocysteine methyltransferase
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homocysteine methylase
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homocysteine methyltransferase
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homocysteine transmethylase
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L-homocysteine S-methyltransferase
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-
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methylmethionine:homocysteine methyltransferase
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-
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S-adenosyl-L-methionine:L-homocysteine methyltransferase
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S-adenosylmethionine homocysteine transmethylase
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S-adenosylmethionine-homocysteine transmethylase
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S-adenosylmethionine:homocysteine methyltransferase
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S-methylmethionine homocysteine transmethylase
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
methyl group transfer
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SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:L-homocysteine S-methyltransferase
The enzyme uses S-adenosyl-L-methionine as methyl donor less actively than S-methyl-L-methionine.
CAS REGISTRY NUMBER
COMMENTARY hide
9012-40-2
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
betaine + L-homocysteine
dimethylglycine + L-methionine
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + L-homocysteine
S-adenosyl-L-homocysteine + L-methionine
show the reaction diagram
S-methyl-L-methionine + L-homocysteine
?
show the reaction diagram
-
S-methyl-L-methionine more effective methyl donor than S-adenosyl-L-methionine
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-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + L-homocysteine
S-adenosyl-L-homocysteine + L-methionine
show the reaction diagram
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0044
mutant K10A, pH 7.5, 37°C
0.0047
mutant K8A, pH 7.5, 37°C
0.0109
mutant K7A, pH 7.5, 37°C
0.01215
wild-type, pH 7.5, 37°C
0.0008
-
kidney, S-methyl-L-methionine as methyl donor
0.0022
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liver, S-adenosyl-L-methionine as methyl donor
0.0087
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liver, S-methyl-L-methionine as methyl donor
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
low expression level
Manually annotated by BRENDA team
low expression level
Manually annotated by BRENDA team
high expression level
Manually annotated by BRENDA team
highest expression level detected
Manually annotated by BRENDA team
low expression level
Manually annotated by BRENDA team
low expression level
Manually annotated by BRENDA team
low expression level
Manually annotated by BRENDA team
-
low activity
Manually annotated by BRENDA team
-
high activity
Manually annotated by BRENDA team
additional information
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no activity detected in brain and heart
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
mainly cytoplasmic localization in liver
Manually annotated by BRENDA team
in most tissues, low expression of BHMT coincides with a preferential nuclear localization of the protein
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
oxidative stress associated with D-galactosamine (Gal) or buthionine sulfoximine (BSO) treatments induces BHMT translocation from cytoplasm to nucleus, which is prevented by administration of N-acetylcysteine and glutathione ethyl ester, respectively. The hepatic nuclear accumulation induced by Gal associates with reduced nuclear BHMT activity and a trend towards increased protein homocysteinylation
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
BHMT1_RAT
407
0
44976
Swiss-Prot
-
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 45000, SDS-PAGE
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
K10A
about 40% of wild-type activity
K7A
slight decrease in activity
K84A
about 45% of wild-type activity
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Shapiro, S.K.; Yphantis, D.A.
Assay of S-methylmethionine and S-adenosylmethionine homocysteine transmethylases
Biochim. Biophys. Acta
36
241-244
1959
Klebsiella aerogenes, Saccharomyces cerevisiae, Escherichia coli, Rattus norvegicus
Manually annotated by BRENDA team
Shapiro, S.K.
S-Adenosylmethionine:L-homocysteine S-methyltransferase (Saccharomyces cerevisiae)
Methods Enzymol.
17B
400-405
1971
Klebsiella aerogenes, Saccharomyces cerevisiae, Rattus norvegicus
-
Manually annotated by BRENDA team
Perez-Miguelsanz, J.; Vallecillo, N.; Garrido, F.; Reytor, E.; Perez-Sala, D.; Pajares, M.A.
Betaine homocysteine S-methyltransferase emerges as a new player of the nuclear methionine cycle
Biochim. Biophys. Acta
1864
1165-1182
2017
Rattus norvegicus (O09171)
Manually annotated by BRENDA team