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Information on EC 1.97.1.1 - chlorate reductase and Organism(s) Ideonella dechloratans and UniProt Accession P60068

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IUBMB Comments
Flavins or benzylviologen can act as acceptor.
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This record set is specific for:
Ideonella dechloratans
UNIPROT: P60068
Word Map
The taxonomic range for the selected organisms is: Ideonella dechloratans
The expected taxonomic range for this enzyme is: Bacteria, Archaea
Synonyms
chlorate reductase, chlorate reductase C, clr, ClrA, NarGH, perchlorate reductase, reductase, chlorate, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
chlorate reductase
189170
-
chlorate reductase C
-
-
-
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perchlorate reductase
-
-
-
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reductase, chlorate
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
redox reaction
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-
-
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PATHWAY SOURCE
PATHWAYS
SYSTEMATIC NAME
IUBMB Comments
chlorite:acceptor oxidoreductase
Flavins or benzylviologen can act as acceptor.
CAS REGISTRY NUMBER
COMMENTARY hide
60382-73-2
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
AH2 + chlorate
A + H2O + chlorite
show the reaction diagram
-
the 6 kDa periplasmatic cytochrome c is able to act as an electron donor in the reaction
-
-
?
bromate + reduced electron acceptor
bromite + H2O + oxidized electron acceptor
show the reaction diagram
-
-
-
-
?
chlorate + reduced electron acceptor
chlorite + H2O + oxidized electron acceptor
show the reaction diagram
ferricytochrome c + chlorate
ferrocytochrome c + H2O + chlorite
show the reaction diagram
P60068; P60069; P60000
-
-
-
?
reduced acceptor + chlorate
acceptor + H2O + chlorite
show the reaction diagram
additional information
?
-
P60068; P60069; P60000
a c-type cytochrome from Ideonella dechloratans donates electrons to Clr in vitro
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-
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
AH2 + chlorate
A + H2O + chlorite
show the reaction diagram
-
the 6 kDa periplasmatic cytochrome c is able to act as an electron donor in the reaction
-
-
?
chlorate + reduced electron acceptor
chlorite + H2O + oxidized electron acceptor
show the reaction diagram
-
important enzyme in bacterial treatment of bleach effluents in pulp and paper industry
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-
?
reduced acceptor + chlorate
acceptor + H2O + chlorite
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
cytochrome c
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Fe2+
P60068; P60069; P60000
in cytochrome c
Iron
-
non-heme iron
Molybdenum
-
bound to enzyme
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
-
activity assay
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.6
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calculated pI of expressed GST-fusion protein
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
subunit alpha
UniProt
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
additional information
P60068; P60069; P60000
the genes for the enzymes chlorate reductase (clrABDC) and chlorite dismutase, necessary for chlorate metabolism and probably acquired by lateral gene transfer, are located in a gene cluster that also includes other genes potentially important for chlorate metabolism. Among those are a gene for cytochrome c (cyc) whose gene product may serve as an electron carrier during chlorate reduction, a cofactor biosynthesis gene (mobB) and a predicted transcriptional regulator (arsR)
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
Sequence
CLRA_IDEDE
914
0
102823
Swiss-Prot
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
inclusion bodies from Escherichia coli and native enzyme
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periplasmatic extract is prepared
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
C-subunit expressed as GST-fusion protein in Escherichia coli BL21
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gene cluster clrABDC, genetic organization, chlorate reductase is cotranscribed with cytochrome c, UniProt ID B3VLA9, and other downstream genes in the gene cluster for chlorate respiration of Ideonella dechloratans. In vivo production of a single polycistronic transcript covering eight open reading frames including clrABDC, cyc, mobB and arsR. Transcription levels of the cyc and clrA genes are compared to each other by the use of qRT-PCR in RNA preparations from cells grown under aerobic or chlorate reducing anaerobic conditions. The two genes show the same mRNA levels under both growth regimes, indicating that no transcription termination occurs between them. Higher transcription levels are observed at growth without external oxygen supply. Quantitative real-time RT-PCR expression analysis
P60068; P60069; P60000
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
both chlorite dismutase and chlorate reductase are expressed during aerobic growth. Transfer to anaerobic conditions with chlorate results in significantly enhanced enzyme activities and mRNA levels for both enzymes. Absence of oxygen is necessary for the induction to occur. Chlorate reductase shows about 200-times higher enzyme activity in anaerobically induced cells, whereas the increase in mRNA is only about 10fold
RENATURED/Commentary
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Karlsson, J.; Nilsson, T.
The C subunit of Ideonella dechloratans chlorate reductase: expression, purification, refolding, and heme reconstitution
Protein Expr. Purif.
41
306-312
2005
Ideonella dechloratans
Manually annotated by BRENDA team
Baecklund, A.S.; Bohlin, J.; Gustavsson, N.; Nilsson, T.
Periplasmic c cytochromes and chlorate reduction in Ideonella dechloratans
Appl. Environ. Microbiol.
75
2439-2445
2009
Ideonella dechloratans
Manually annotated by BRENDA team
Lindqvist, M.H.; Johansson, N.; Nilsson, T.; Rova, M.
Expression of chlorite dismutase and chlorate reductase in the presence of oxygen and/or chlorate as the terminal electron acceptor in Ideonella dechloratans
Appl. Environ. Microbiol.
78
4380-4385
2012
Ideonella dechloratans, Ideonella dechloratans (P60068)
Manually annotated by BRENDA team
Liebensteiner, M.G.; Oosterkamp, M.J.; Stams, A.J.M.
Microbial respiration with chlorine oxyanions diversity and physiological and biochemical properties of chlorate and perchlorate-reducing microorganisms
Ann. N. Y. Acad. Sci.
1365
59-72
2016
Acinetobacter thermotoleranticus, Alicycliphilus denitrificans, Alicycliphilus denitrificans BC, Archaeoglobus fulgidus, Archaeoglobus fulgidus VC-16, Arcobacter sp. CAB, Azospira oryzae, Azospira oryzae GR-1, Azospira sp., Azospira sp. Perc1ace, Azospirillum lipoferum, Azospirillum lipoferum VPI Sp 59b, Dechloromonas agitata, Dechloromonas agitata CKB, Dechloromonas aromatica, Dechloromonas aromatica RCB, Dechloromonas hortensis, Dechloromonas hortensis MA-1, Dechloromonas sp., Dechloromonas sp. JM, Dechlorospirillum anomalous, Dechlorospirillum anomalous 7JB116, Haloarcula marismortui, Haloarcula vallismortis, Haloferax denitrificans, Haloferax denitrificans S1, Haloferax gibbonsii, Haloferax gibbonsii Ma 2.38, Haloferax mediterranei, Haloferax mediterranei R-4, Haloferax volcanii, Haloferax volcanii DS2, Ideonella dechloratans, Ideonella dechloratans CCUG 30898, Marinobacter vinifirmus, Marinobacter vinifirmus P4B1, Moorella glycerini, Moorella glycerini JW/AS-Y6, Moorella humiferrea, Moorella humiferrea 64-FGQ, Moorella mulderi, Moorella mulderi TMS, Moorella perchloratireducens, Moorella perchloratireducens An10, Moorella stamsii, Moorella stamsii E3-O, no activity in Dechloromonas aromatica, Pseudomonas chloritidismutans, Pseudomonas chloritidismutans ASK1, Pseudomonas chloritidismutans AW1, Sedimenticola selenatireducens, Sedimenticola selenatireducens CUZ, Sedimenticola selenatireducens NSS, Shewanella algae, Shewanella algae ACDC, Sporomusa ovata, Sporomusa ovata An4, Sulfurospirillum multivorans, Vibrio dechloraticans, Vibrio dechloraticans Cuznesove B-1168, Wolinella succinogenes, Wolinella succinogenes HAP-1
Manually annotated by BRENDA team
Hellberg Lindqvist, M.; Nilsson, T.; Sundin, P.; Rova, M.
Chlorate reductase is cotranscribed with cytochrome c and other downstream genes in the gene cluster for chlorate respiration of Ideonella dechloratans
FEMS Microbiol. Lett.
362
fnv019
2015
Ideonella dechloratans, Ideonella dechloratans (P60068 AND P60069 AND P60000)
Manually annotated by BRENDA team
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