BRENDA - Enzyme Database
show all sequences of 1.97.1.1

Chlorate reductase is cotranscribed with cytochrome c and other downstream genes in the gene cluster for chlorate respiration of Ideonella dechloratans

Hellberg Lindqvist, M.; Nilsson, T.; Sundin, P.; Rova, M.; FEMS Microbiol. Lett. 362, fnv019 (2015)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene cluster clrABDC, genetic organization, chlorate reductase is cotranscribed with cytochrome c, UniProt ID B3VLA9, and other downstream genes in the gene cluster for chlorate respiration of Ideonella dechloratans. In vivo production of a single polycistronic transcript covering eight open reading frames including clrABDC, cyc, mobB and arsR. Transcription levels of the cyc and clrA genes are compared to each other by the use of qRT-PCR in RNA preparations from cells grown under aerobic or chlorate reducing anaerobic conditions. The two genes show the same mRNA levels under both growth regimes, indicating that no transcription termination occurs between them. Higher transcription levels are observed at growth without external oxygen supply. Quantitative real-time RT-PCR expression analysis
Ideonella dechloratans
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe2+
in cytochrome c
Ideonella dechloratans
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
reduced acceptor + chlorate
Ideonella dechloratans
-
acceptor + H2O + chlorite
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Ideonella dechloratans
P60068 AND P60069 AND P60000
subunits alpha, beta, and gamma
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ferricytochrome c + chlorate
-
744933
Ideonella dechloratans
ferrocytochrome c + H2O + chlorite
-
-
-
?
additional information
a c-type cytochrome from Ideonella dechloratans donates electrons to Clr in vitro
744933
Ideonella dechloratans
?
-
-
-
-
reduced acceptor + chlorate
-
744933
Ideonella dechloratans
acceptor + H2O + chlorite
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome c
-
Ideonella dechloratans
Cloned(Commentary) (protein specific)
Commentary
Organism
gene cluster clrABDC, genetic organization, chlorate reductase is cotranscribed with cytochrome c, UniProt ID B3VLA9, and other downstream genes in the gene cluster for chlorate respiration of Ideonella dechloratans. In vivo production of a single polycistronic transcript covering eight open reading frames including clrABDC, cyc, mobB and arsR. Transcription levels of the cyc and clrA genes are compared to each other by the use of qRT-PCR in RNA preparations from cells grown under aerobic or chlorate reducing anaerobic conditions. The two genes show the same mRNA levels under both growth regimes, indicating that no transcription termination occurs between them. Higher transcription levels are observed at growth without external oxygen supply. Quantitative real-time RT-PCR expression analysis
Ideonella dechloratans
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome c
-
Ideonella dechloratans
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe2+
in cytochrome c
Ideonella dechloratans
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
reduced acceptor + chlorate
Ideonella dechloratans
-
acceptor + H2O + chlorite
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ferricytochrome c + chlorate
-
744933
Ideonella dechloratans
ferrocytochrome c + H2O + chlorite
-
-
-
?
additional information
a c-type cytochrome from Ideonella dechloratans donates electrons to Clr in vitro
744933
Ideonella dechloratans
?
-
-
-
-
reduced acceptor + chlorate
-
744933
Ideonella dechloratans
acceptor + H2O + chlorite
-
-
-
?
General Information
General Information
Commentary
Organism
additional information
the genes for the enzymes chlorate reductase (clrABDC) and chlorite dismutase, necessary for chlorate metabolism and probably acquired by lateral gene transfer, are located in a gene cluster that also includes other genes potentially important for chlorate metabolism. Among those are a gene for cytochrome c (cyc) whose gene product may serve as an electron carrier during chlorate reduction, a cofactor biosynthesis gene (mobB) and a predicted transcriptional regulator (arsR)
Ideonella dechloratans
physiological function
a soluble periplasmic c-type cytochrome from Ideonella dechloratans donates electrons to Clr in vitro
Ideonella dechloratans
General Information (protein specific)
General Information
Commentary
Organism
additional information
the genes for the enzymes chlorate reductase (clrABDC) and chlorite dismutase, necessary for chlorate metabolism and probably acquired by lateral gene transfer, are located in a gene cluster that also includes other genes potentially important for chlorate metabolism. Among those are a gene for cytochrome c (cyc) whose gene product may serve as an electron carrier during chlorate reduction, a cofactor biosynthesis gene (mobB) and a predicted transcriptional regulator (arsR)
Ideonella dechloratans
physiological function
a soluble periplasmic c-type cytochrome from Ideonella dechloratans donates electrons to Clr in vitro
Ideonella dechloratans
Other publictions for EC 1.97.1.1
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
744044
Liebensteiner
Microbial respiration with ch ...
Acinetobacter thermotoleranticus, Alicycliphilus denitrificans, Alicycliphilus denitrificans BC, Archaeoglobus fulgidus, Archaeoglobus fulgidus VC-16, Arcobacter sp. CAB, Azospira oryzae, Azospira oryzae GR-1, Azospira sp., Azospira sp. Perc1ace, Azospirillum lipoferum, Azospirillum lipoferum VPI Sp 59b, Dechloromonas agitata, Dechloromonas agitata CKB, Dechloromonas aromatica, Dechloromonas aromatica RCB, Dechloromonas hortensis, Dechloromonas hortensis MA-1, Dechloromonas sp., Dechloromonas sp. JM, Dechlorospirillum anomalous, Dechlorospirillum anomalous 7JB116, Haloarcula marismortui, Haloarcula vallismortis, Haloferax denitrificans, Haloferax denitrificans S1, Haloferax gibbonsii, Haloferax gibbonsii Ma 2.38, Haloferax mediterranei, Haloferax mediterranei R-4, Haloferax volcanii, Haloferax volcanii DS2, Ideonella dechloratans, Ideonella dechloratans CCUG 30898, Marinobacter vinifirmus, Marinobacter vinifirmus P4B1, Moorella glycerini, Moorella glycerini JW/AS-Y6, Moorella humiferrea, Moorella humiferrea 64-FGQ, Moorella mulderi, Moorella mulderi TMS, Moorella perchloratireducens, Moorella perchloratireducens An10, Moorella stamsii, Moorella stamsii E3-O, no activity in Dechloromonas aromatica, Pseudomonas chloritidismutans, Pseudomonas chloritidismutans ASK1, Pseudomonas chloritidismutans AW1, Sedimenticola selenatireducens, Sedimenticola selenatireducens CUZ, Sedimenticola selenatireducens NSS, Shewanella algae, Shewanella algae ACDC, Sporomusa ovata, Sporomusa ovata An4, Sulfurospirillum multivorans, Vibrio dechloraticans, Vibrio dechloraticans Cuznesove B-1168, Wolinella succinogenes, Wolinella succinogenes HAP-1
Ann. N. Y. Acad. Sci.
1365
59-72
2016
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93
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75
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20
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93
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15
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93
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20
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32
32
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-
729317
Martínez-Espinosa
Characterisation of chlorate r ...
Haloferax mediterranei, Haloferax mediterranei ATCC 33500
Biochim. Biophys. Acta
1850
587-594
2015
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744933
Hellberg Lindqvist
Chlorate reductase is cotrans ...
Ideonella dechloratans
FEMS Microbiol. Lett.
362
fnv019
2015
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2
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726725
Lindqvist
Expression of chlorite dismuta ...
Ideonella dechloratans
Appl. Environ. Microbiol.
78
4380-4385
2012
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1
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1
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713857
Bansal
Peptide Biomarkers as Evidence ...
Azospira oryzae, Dechloromonas agitata, Dechloromonas hortensis, Dechlorosoma sp., Dechlorospirillum anomalous, Dechlorospirillum anomalous NCCB100047
Appl. Environ. Microbiol.
77
810-820
2011
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5
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713887
Balk
(Per)chlorate reduction by an ...
Sporomusa sp., Sporomusa sp. An4
Appl. Microbiol. Biotechnol.
88
595-603
2010
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695767
Baecklund
Periplasmic c cytochromes and ...
Ideonella dechloratans
Appl. Environ. Microbiol.
75
2439-2445
2009
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1
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695834
Mehboob
Growth of Pseudomonas chloriti ...
Pseudomonas chloritidismutans, Pseudomonas chloritidismutans AW-1(T)
Appl. Microbiol. Biotechnol.
83
739-747
2009
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696504
Bansal
Proteomic detection of protein ...
Dechloromonas hortensis, Dechlorosoma sp., Dechlorosoma sp. KJ / ATCC BAA-592
Biodegradation
20
603-620
2009
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2
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695753
Weelink
Isolation and characterization ...
Alicycliphilus denitrificans, Alicycliphilus denitrificans BC, Alicycliphilus sp.
Appl. Environ. Microbiol.
74
6672-6681
2008
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1
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3
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1
-
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658779
Steinberg
Enzymes responsible for chlora ...
Dechlorosoma sp., Dechlorosoma sp. KJ / ATCC BAA-592, Pseudomonas sp., Pseudomonas sp. PDA
FEMS Microbiol. Lett.
247
153-159
2005
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2
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660446
Karlsson
The C subunit of Ideonella dec ...
Ideonella dechloratans
Protein Expr. Purif.
41
306-312
2005
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674244
Bender
Identification, characterizati ...
Dechloromonas agitata
J. Bacteriol.
187
5090-5096
2005
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659023
Wolterink
Characterization of the chlora ...
Pseudomonas chloritidismutans, Pseudomonas chloritidismutans AW-1 / DSM 13592
J. Bacteriol.
185
3210-3213
2003
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3
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437686
Giblin
Perchlorate and nitrate reduct ...
bacterium perc1ace
Microbiol. Res.
156
311-315
2001
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437685
Kengen
Purification and characterizat ...
Azospira oryzae, Azospira oryzae GR-1
J. Bacteriol.
181
6706-6711
1999
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437684
Roldan
-
Chlorate and nitrate reduction ...
Rhodobacter capsulatus, Rhodobacter sphaeroides
Curr. Microbiol.
29
241-245
1994
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437682
Oltmann
Influence of tungstate on the ...
Proteus mirabilis
FEBS Lett.
106
43-46
1979
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437681
Oltmann
-
Characterization of purified n ...
Proteus mirabilis
Arch. Microbiol.
1111
25-35
1976
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437683
Azoulay
Study of chlorate-resistant mu ...
Escherichia coli
Biochim. Biophys. Acta
237
579-590
1971
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