We're sorry, but BRENDA doesn't work properly without JavaScript. Please make sure you have JavaScript enabled in your browser settings.
Information on EC 1.8.5.2 - thiosulfate dehydrogenase (quinone) for references in articles please use BRENDA:EC1.8.5.2
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB Comments The reaction can also proceed with ferricyanide as the electron acceptor, but more slowly. Unlike EC 1.8.2.2, thiosulfate dehydrogenase, this enzyme cannot utilize cytochrome c as an acceptor.
The expected taxonomic range for this enzyme is: Archaea, Bacteria
Synonyms
tetrathionate-forming thiosulfate dehydrogenase, thiosulfate oxidoreductase, thiosulfate oxidoreductase tetrathionate-forming, thiosulfate:quinone oxidoreductase, thiosulphate:quinone oxidoreductase, TQO, TSD,
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
tetrathionate-forming thiosulfate dehydrogenase
-
-
thiosulfate oxidoreductase
-
thiosulfate oxidoreductase tetrathionate-forming
-
thiosulfate:quinone oxidoreductase
-
thiosulphate:quinone oxidoreductase
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
2 thiosulfate + 6-decylubiquinone = tetrathionate + 6-decylubiquinol
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
thiosulfate:6-decylubiquinone oxidoreductase
The reaction can also proceed with ferricyanide as the electron acceptor, but more slowly. Unlike EC 1.8.2.2, thiosulfate dehydrogenase, this enzyme cannot utilize cytochrome c as an acceptor.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
2 thiosulfate + caldariellaquinone + 2 H+
tetrathionate + caldariellaquinol
the physiological electron acceptor is most probably a caldariella quinone type quinone
-
-
r
tetrathionate + reduced methylene blue
thiosulfate + oxidized methylene blue
-
-
-
r
thiosulfate + 6-decylubiquinone
tetrathionate + 6-decylubiquinol
thiosulfate + ferricyanide
tetrathionate + ferrocyanide
thiosulfate + ferricyanide + H+
tetrathionate + ferrocyanide
-
when the concentration of thiosulfate is lower than 4 mM, no enzyme activity is detected
-
-
?
additional information
?
-
thiosulfate + 6-decylubiquinone
tetrathionate + 6-decylubiquinol
-
-
-
?
thiosulfate + 6-decylubiquinone
tetrathionate + 6-decylubiquinol
-
-
-
r
thiosulfate + 6-decylubiquinone
tetrathionate + 6-decylubiquinol
the enzyme couples sulfur compound oxidation with quinone reduction
-
-
?
thiosulfate + ferricyanide
tetrathionate + ferrocyanide
-
-
-
?
thiosulfate + ferricyanide
tetrathionate + ferrocyanide
-
-
-
r
additional information
?
-
-
horse heart cytochrome c is not reduced
-
-
-
additional information
?
-
horse heart cytochrome c is not reduced
-
-
-
additional information
?
-
-
the enzyme reduces neither ubiquinone nor horse heart cytochrome c, which serves as an electron acceptor
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
2 thiosulfate + caldariellaquinone + 2 H+
tetrathionate + caldariellaquinol
the physiological electron acceptor is most probably a caldariella quinone type quinone
-
-
r
thiosulfate + 6-decylubiquinone
tetrathionate + 6-decylubiquinol
the enzyme couples sulfur compound oxidation with quinone reduction
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
additional information
a mixture of caldariella quinone, Sulfolobus quinone and menaquinone is non-covalently bound to the protein
-
additional information
-
does not contain heme
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Dithionite
; 1 mM, complete inhibition
Metabisulfite
; 1 mM, complete inhibition
N-ethylmaleimide
1 mM, 54% inhibition
reduced titanium citrate
1 mM, 65% inhibition
Sodium sulfate
-
60% reduction in enzyme activity is observed in the reaction mixture containing 200 mM sodium sulfate
Triton X-100
; 1%, complete inhibition
Zn2+
; 1 mM, 37% inhibition, 5 mM, 82% inhibition
additional information
no inhibition by sulfate or tetrathionate
-
sulfite
; 0.005 mM, 48% inhibition, 0.05 mM, complete inhibition
sulfite
-
almost complete inhibition in the presence of 10 mM sulfite
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Sodium sulfate
-
the purified enzyme requires 150 mM sodium sulfate to generate maximum activity
sulfite
-
the addition of sulfite (2 mM) results in the activation of the enzyme at a magnitude of 1.6fold
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.00587 - 0.0059
6-decylubiquinone
3.4
ferricyanide
; 80°C, pH 6.0
0.00587
6-decylubiquinone
-
0.0059
6-decylubiquinone
80°C, pH 6.0
2.6
thiosulfate
; 80°C, pH 6.0
8
thiosulfate
-
recombinant enzyme, at pH 2.5 and 50°C
15
thiosulfate
-
native enzyme, at pH 2.5 and 70°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
167
thiosulfate
; 80°C, pH 6.0
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.005
sulfite
; pH 6.0, 80°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.05
-
unpurified recombinant enzyme, at pH 2.5 and 40°C
0.397
activity with decyl ubiquinone at pH 6, 92°C
3.16
-
recombinant enzyme after 63.2fold purification, at pH 2.5 and 40°C
397
with 6-decylubiquinone as electron acceptor
49.9
with ferricyanide as electron acceptor
73.4
activity with ferricyanide at pH 6, 92°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
2.5
-
native and recombinant enzyme
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
2 - 3
-
the native enzyme shows about 35% activity at pH 2.0 and 20% activity at pH 3.0, the recombinant enzyme shows about 80% activity at pH 2.0 and about 35% activity at pH 3.0
3.5 - 6.8
approx. 55% of maximal activity at pH 3.5, approx. 22% of maximal activity at pH 6.5
4.5 - 5.5
pH 4.5: about 70% of maximal activity, pH 5.5: about 35% of maximal activity
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
20 - 92
increasing activity is observed in the range between 20 and 92°C, a maximum is not observed because of technical reasons
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
20 - 92
increasing activity is observed in the range between 20 and 92°C, a maximum is not observed because of technical reasons
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
UnIProt
brenda
-
-
-
brenda
-
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
brenda
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
DOXA_ACIAM
168
1
18757
Swiss-Prot
DOXD_ACIAM
184
4
20410
Swiss-Prot
A0A0B8N5Y9_9NOCA
151
0
15133
TrEMBL
A0A5E6MDK8_9BACT
150
4
15999
TrEMBL
A0A088E3I8_9CREN
166
1
18421
TrEMBL
A0A2W0D2Y3_9BACL
132
4
13566
TrEMBL
A0A081S6L4_9ARCH
140
3
15212
TrEMBL
A0A087S200_9ARCH
139
4
14490
TrEMBL
A0A5E6M613_9BACT
161
3
17777
TrEMBL
A0A1B2GTK3_STRNR
149
4
16751
TrEMBL
A4YDN9_METS5
Metallosphaera sedula (strain ATCC 51363 / DSM 5348 / JCM 9185 / NBRC 15509 / TH2)
166
1
18421
TrEMBL
G0L8D0_ZOBGA
Zobellia galactanivorans (strain DSM 12802 / CCUG 47099 / CIP 106680 / NCIMB 13871 / Dsij)
338
5
37833
TrEMBL
A0A5E6MDA4_9BACT
149
4
15713
TrEMBL
A0A088E409_9CREN
182
3
20042
TrEMBL
A0A1E3L3Y2_9BACL
174
4
19043
TrEMBL
A0A081RPE0_9ARCH
140
3
15212
TrEMBL
A0A081RLD8_9ARCH
139
4
14490
TrEMBL
A0A2W0CA50_9BACL
136
4
14432
TrEMBL
A0A087RRL5_9ARCH
140
3
15212
TrEMBL
A0A5E6MFJ9_9BACT
165
3
18457
TrEMBL
A0A087RYC8_9ARCH
139
4
14490
TrEMBL
A0A2W0CU64_9BACL
179
3
19631
TrEMBL
Q96ZH9_SULTO
Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7)
180
3
19909
TrEMBL
A0A1B2GSB8_STRNR
177
2
18656
TrEMBL
A0A087S953_9ARCH
139
4
14490
TrEMBL
A0A081S6J7_9ARCH
139
4
14490
TrEMBL
A4YDN8_METS5
Metallosphaera sedula (strain ATCC 51363 / DSM 5348 / JCM 9185 / NBRC 15509 / TH2)
182
3
20042
TrEMBL
F9VNN5_SULTO
Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7)
168
1
18410
TrEMBL
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
16000
alpha2,beta2, 2 * 16000 + 2 * 28000, SDS-PAGE; alpha2beta2, the 28000 Da subunit and the 16000 Da subunit are identical to to DoxA and DoxD from Acidianus ambivalens quinol:oxygen oxidoreductase, 2 * 16000 + 2 * 28000, SDS-PAGE
18700
alpha2,beta2, 2 * 20400 + 2 * 18700, deduced from nucleotide sequence
20400
alpha2,beta2, 2 * 20400 + 2 * 18700, deduced from nucleotide sequence
25000
-
x * 25000, SDS-PAGE
28000
alpha2,beta2, 2 * 16000 + 2 * 28000, SDS-PAGE; alpha2beta2, the 28000 Da subunit and the 16000 Da subunit are identical to to DoxA and DoxD from Acidianus ambivalens quinol:oxygen oxidoreductase, 2 * 16000 + 2 * 28000, SDS-PAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
tetramer
alpha2,beta2, 2 * 16000 + 2 * 28000, SDS-PAGE; alpha2,beta2, 2 * 20400 + 2 * 18700, deduced from nucleotide sequence; alpha2beta2, the 28000 Da subunit and the 16000 Da subunit are identical to to DoxA and DoxD from Acidianus ambivalens quinol:oxygen oxidoreductase, 2 * 16000 + 2 * 28000, SDS-PAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
glycoprotein
the larger subunit (28000 Da) appears to be glycosylated
phosphoprotein
glycosylation of subunit DoxA, i.e. beta subunit of TQO
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
; solubilization, Q-Sepharose, hydroxylapatite, DEAE-Sepharose
affinity matrix HR 5/5 column chromatography, matrices based on cytochrome c immobilized on crosslinked triazine (2,4,6-tris(aminoethylamine)-1,3,5-triazine), cytochrome c immobilized on Silasorb-amine with carbodiimide activation and cytochrome c immobilized on Sepharose CL-4B
-
ammonium sulfate precipitation, CM-650M column chromatography, butyl-650M column chromatography, and TSKgel G3000 gel filtration
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
expressed in Escherichia coli BL21(DE3) cells
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Muller, F.H.; Bandeiras, T.M.; Urich, T.; Teixeira, M.; Gomes, C.M.; Kletzin, A.
Coupling of the pathway of sulphur oxidation to dioxygen reduction: characterization of a novel membrane-bound thiosulphate:quinone oxidoreductase
Mol. Microbiol.
53
1147-1160
2004
Acidianus ambivalens, Acidianus ambivalens (P97224 AND P97207)
brenda
Janiczek, O.; Pokorna, B.; Zemanova, J.; Mandl, M.
Use of immobilized cytochrome c as a ligand for affinity chromatography of thiosulfate dehydrogenase from Acidithiobacillus ferrooxidans
J. Biotechnol.
117
293-298
2005
Acidithiobacillus ferrooxidans, Acidithiobacillus ferrooxidans CCM 4253
brenda
Kikumoto, M.; Nogami, S.; Kanao, T.; Takada, J.; Kamimura, K.
Tetrathionate-forming thiosulfate dehydrogenase from the acidophilic, chemolithoautotrophic bacterium Acidithiobacillus ferrooxidans
Appl. Environ. Microbiol.
79
113-120
2013
Acidithiobacillus ferrooxidans
brenda
Select items on the left to see more content.
html completed
HOME
login
history
all enzymes
BRENDA home
History
1.8.5.2 thiosulfate dehydrogenase (quinone)
more
top
Information
