Data extracted from this reference:
Activating Compound
Sodium sulfate
the purified enzyme requires 150 mM sodium sulfate to generate maximum activity
Acidithiobacillus ferrooxidans
sulfite
the addition of sulfite (2 mM) results in the activation of the enzyme at a magnitude of 1.6fold
Acidithiobacillus ferrooxidans
Cloned(Commentary)
expressed in Escherichia coli BL21(DE3) cells
Acidithiobacillus ferrooxidans
Inhibitors
Sodium sulfate
60% reduction in enzyme activity is observed in the reaction mixture containing 200 mM sodium sulfate
Acidithiobacillus ferrooxidans
sulfite
almost complete inhibition in the presence of 10 mM sulfite
Acidithiobacillus ferrooxidans
KM Value [mM]
8
thiosulfate
recombinant enzyme, at pH 2.5 and 50°C
Acidithiobacillus ferrooxidans
15
thiosulfate
native enzyme, at pH 2.5 and 70°C
Acidithiobacillus ferrooxidans
Molecular Weight [Da]
25000
x * 25000, SDS-PAGE
Acidithiobacillus ferrooxidans
Organism
Acidithiobacillus ferrooxidans
Purification (Commentary)
ammonium sulfate precipitation, CM-650M column chromatography, butyl-650M column chromatography, and TSKgel G3000 gel filtration
Acidithiobacillus ferrooxidans
Source Tissue
culture condition:sulfur-grown cell
Acidithiobacillus ferrooxidans
culture condition:tetrathionate-grown cell
Acidithiobacillus ferrooxidans
Specific Activity [micromol/min/mg]
0.05
unpurified recombinant enzyme, at pH 2.5 and 40°C
Acidithiobacillus ferrooxidans
3.16
recombinant enzyme after 63.2fold purification, at pH 2.5 and 40°C
Acidithiobacillus ferrooxidans
Substrates and Products (Substrate)
additional information
the enzyme reduces neither ubiquinone nor horse heart cytochrome c, which serves as an electron acceptor
724028
Acidithiobacillus ferrooxidans
?
thiosulfate + ferricyanide + H+
when the concentration of thiosulfate is lower than 4 mM, no enzyme activity is detected
724028
Acidithiobacillus ferrooxidans
tetrathionate + ferrocyanide
?
Subunits
?
x * 25000, SDS-PAGE
Acidithiobacillus ferrooxidans
Synonyms
tetrathionate-forming thiosulfate dehydrogenase
Acidithiobacillus ferrooxidans
TSD
Acidithiobacillus ferrooxidans
Temperature Optimum [°C]
50
recombinant enzyme
Acidithiobacillus ferrooxidans
70
native enzyme
Acidithiobacillus ferrooxidans
pH Optimum
2.5
native and recombinant enzyme
Acidithiobacillus ferrooxidans
pH Range
2
3
the native enzyme shows about 35% activity at pH 2.0 and 20% activity at pH 3.0, the recombinant enzyme shows about 80% activity at pH 2.0 and about 35% activity at pH 3.0
Acidithiobacillus ferrooxidans
Cofactor
additional information
does not contain heme
Acidithiobacillus ferrooxidans
Activating Compound (protein specific)
Sodium sulfate
the purified enzyme requires 150 mM sodium sulfate to generate maximum activity
Acidithiobacillus ferrooxidans
sulfite
the addition of sulfite (2 mM) results in the activation of the enzyme at a magnitude of 1.6fold
Acidithiobacillus ferrooxidans
Cloned(Commentary) (protein specific)
expressed in Escherichia coli BL21(DE3) cells
Acidithiobacillus ferrooxidans
Cofactor (protein specific)
additional information
does not contain heme
Acidithiobacillus ferrooxidans
Inhibitors (protein specific)
Sodium sulfate
60% reduction in enzyme activity is observed in the reaction mixture containing 200 mM sodium sulfate
Acidithiobacillus ferrooxidans
sulfite
almost complete inhibition in the presence of 10 mM sulfite
Acidithiobacillus ferrooxidans
KM Value [mM] (protein specific)
8
thiosulfate
recombinant enzyme, at pH 2.5 and 50°C
Acidithiobacillus ferrooxidans
15
thiosulfate
native enzyme, at pH 2.5 and 70°C
Acidithiobacillus ferrooxidans
Molecular Weight [Da] (protein specific)
25000
x * 25000, SDS-PAGE
Acidithiobacillus ferrooxidans
Purification (Commentary) (protein specific)
ammonium sulfate precipitation, CM-650M column chromatography, butyl-650M column chromatography, and TSKgel G3000 gel filtration
Acidithiobacillus ferrooxidans
Source Tissue (protein specific)
culture condition:sulfur-grown cell
Acidithiobacillus ferrooxidans
culture condition:tetrathionate-grown cell
Acidithiobacillus ferrooxidans
Specific Activity [micromol/min/mg] (protein specific)
0.05
unpurified recombinant enzyme, at pH 2.5 and 40°C
Acidithiobacillus ferrooxidans
3.16
recombinant enzyme after 63.2fold purification, at pH 2.5 and 40°C
Acidithiobacillus ferrooxidans
Substrates and Products (Substrate) (protein specific)
additional information
the enzyme reduces neither ubiquinone nor horse heart cytochrome c, which serves as an electron acceptor
724028
Acidithiobacillus ferrooxidans
?
thiosulfate + ferricyanide + H+
when the concentration of thiosulfate is lower than 4 mM, no enzyme activity is detected
724028
Acidithiobacillus ferrooxidans
tetrathionate + ferrocyanide
?
Subunits (protein specific)
?
x * 25000, SDS-PAGE
Acidithiobacillus ferrooxidans
Temperature Optimum [°C] (protein specific)
50
recombinant enzyme
Acidithiobacillus ferrooxidans
70
native enzyme
Acidithiobacillus ferrooxidans
pH Optimum (protein specific)
2.5
native and recombinant enzyme
Acidithiobacillus ferrooxidans
pH Range (protein specific)
2
3
the native enzyme shows about 35% activity at pH 2.0 and 20% activity at pH 3.0, the recombinant enzyme shows about 80% activity at pH 2.0 and about 35% activity at pH 3.0
Acidithiobacillus ferrooxidans
Other publictions for EC 1.8.5.2
724028
Kikumoto
Tetrathionate-forming thiosulf ...
Acidithiobacillus ferrooxidans
Appl. Environ. Microbiol.
79
113-120
2013
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659545
Janiczek
Use of immobilized cytochrome ...
Acidithiobacillus ferrooxidans, Acidithiobacillus ferrooxidans CCM 4253
J. Biotechnol.
117
293-298
2005
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656833
Muller
Coupling of the pathway of sul ...
Acidianus ambivalens
Mol. Microbiol.
53
1147-1160
2004
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