BRENDA - Reference to 1.8.5.2; Id = 724028

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Tetrathionate-forming thiosulfate dehydrogenase from the acidophilic, chemolithoautotrophic bacterium Acidithiobacillus ferrooxidans

Kikumoto, M.; Nogami, S.; Kanao, T.; Takada, J.; Kamimura, K.; Appl. Environ. Microbiol. 79, 113-120 (2013)

Data extracted from this reference:

Activating Compound

Sodium sulfate

the purified enzyme requires 150 mM sodium sulfate to generate maximum activity

Acidithiobacillus ferrooxidans

sulfite

the addition of sulfite (2 mM) results in the activation of the enzyme at a magnitude of 1.6fold

Acidithiobacillus ferrooxidans

Cloned(Commentary)

expressed in Escherichia coli BL21(DE3) cells

Acidithiobacillus ferrooxidans

Inhibitors

Sodium sulfate

60% reduction in enzyme activity is observed in the reaction mixture containing 200 mM sodium sulfate

Acidithiobacillus ferrooxidans

sulfite

almost complete inhibition in the presence of 10 mM sulfite

Acidithiobacillus ferrooxidans

KM Value [mM]

8

-

thiosulfate

recombinant enzyme, at pH 2.5 and 50°C

Acidithiobacillus ferrooxidans

15

-

thiosulfate

native enzyme, at pH 2.5 and 70°C

Acidithiobacillus ferrooxidans

Molecular Weight [Da]

25000

-

x * 25000, SDS-PAGE

Acidithiobacillus ferrooxidans

Organism

Acidithiobacillus ferrooxidans

-

-

-

Purification (Commentary)

ammonium sulfate precipitation, CM-650M column chromatography, butyl-650M column chromatography, and TSKgel G3000 gel filtration

Acidithiobacillus ferrooxidans

Source Tissue

culture condition:sulfur-grown cell

-

Acidithiobacillus ferrooxidans

-

culture condition:tetrathionate-grown cell

-

Acidithiobacillus ferrooxidans

-

Specific Activity [micromol/min/mg]

0.05

-

unpurified recombinant enzyme, at pH 2.5 and 40°C

Acidithiobacillus ferrooxidans

3.16

-

recombinant enzyme after 63.2fold purification, at pH 2.5 and 40°C

Acidithiobacillus ferrooxidans

Substrates and Products (Substrate)

additional information

the enzyme reduces neither ubiquinone nor horse heart cytochrome c, which serves as an electron acceptor

724028

Acidithiobacillus ferrooxidans

?

-

-

-

-

thiosulfate + ferricyanide + H+

when the concentration of thiosulfate is lower than 4 mM, no enzyme activity is detected

724028

Acidithiobacillus ferrooxidans

tetrathionate + ferrocyanide

-

-

-

?

Subunits

?

x * 25000, SDS-PAGE

Acidithiobacillus ferrooxidans

Temperature Optimum [°C]

50

-

recombinant enzyme

Acidithiobacillus ferrooxidans

70

-

native enzyme

Acidithiobacillus ferrooxidans

pH Optimum

2.5

-

native and recombinant enzyme

Acidithiobacillus ferrooxidans

pH Range

2

3

the native enzyme shows about 35% activity at pH 2.0 and 20% activity at pH 3.0, the recombinant enzyme shows about 80% activity at pH 2.0 and about 35% activity at pH 3.0

Acidithiobacillus ferrooxidans

Cofactor

additional information

does not contain heme

Acidithiobacillus ferrooxidans

Activating Compound (protein specific)

Sodium sulfate

the purified enzyme requires 150 mM sodium sulfate to generate maximum activity

Acidithiobacillus ferrooxidans

sulfite

the addition of sulfite (2 mM) results in the activation of the enzyme at a magnitude of 1.6fold

Acidithiobacillus ferrooxidans

Cloned(Commentary) (protein specific)

expressed in Escherichia coli BL21(DE3) cells

Acidithiobacillus ferrooxidans

Cofactor (protein specific)

additional information

does not contain heme

Acidithiobacillus ferrooxidans

Inhibitors (protein specific)

Sodium sulfate

60% reduction in enzyme activity is observed in the reaction mixture containing 200 mM sodium sulfate

Acidithiobacillus ferrooxidans

sulfite

almost complete inhibition in the presence of 10 mM sulfite

Acidithiobacillus ferrooxidans

KM Value [mM] (protein specific)

8

-

thiosulfate

recombinant enzyme, at pH 2.5 and 50°C

Acidithiobacillus ferrooxidans

15

-

thiosulfate

native enzyme, at pH 2.5 and 70°C

Acidithiobacillus ferrooxidans

Molecular Weight [Da] (protein specific)

25000

-

x * 25000, SDS-PAGE

Acidithiobacillus ferrooxidans

Purification (Commentary) (protein specific)

ammonium sulfate precipitation, CM-650M column chromatography, butyl-650M column chromatography, and TSKgel G3000 gel filtration

Acidithiobacillus ferrooxidans

Source Tissue (protein specific)

culture condition:sulfur-grown cell

-

Acidithiobacillus ferrooxidans

-

culture condition:tetrathionate-grown cell

-

Acidithiobacillus ferrooxidans

-

Specific Activity [micromol/min/mg] (protein specific)

0.05

-

unpurified recombinant enzyme, at pH 2.5 and 40°C

Acidithiobacillus ferrooxidans

3.16

-

recombinant enzyme after 63.2fold purification, at pH 2.5 and 40°C

Acidithiobacillus ferrooxidans

Substrates and Products (Substrate) (protein specific)

additional information

the enzyme reduces neither ubiquinone nor horse heart cytochrome c, which serves as an electron acceptor

724028

Acidithiobacillus ferrooxidans

?

-

-

-

-

thiosulfate + ferricyanide + H+

when the concentration of thiosulfate is lower than 4 mM, no enzyme activity is detected

724028

Acidithiobacillus ferrooxidans

tetrathionate + ferrocyanide

-

-

-

?

Subunits (protein specific)

?

x * 25000, SDS-PAGE

Acidithiobacillus ferrooxidans

Temperature Optimum [°C] (protein specific)

50

-

recombinant enzyme

Acidithiobacillus ferrooxidans

70

-

native enzyme

Acidithiobacillus ferrooxidans

pH Optimum (protein specific)

2.5

-

native and recombinant enzyme

Acidithiobacillus ferrooxidans

pH Range (protein specific)

2

3

the native enzyme shows about 35% activity at pH 2.0 and 20% activity at pH 3.0, the recombinant enzyme shows about 80% activity at pH 2.0 and about 35% activity at pH 3.0

Acidithiobacillus ferrooxidans

Other publictions for EC 1.8.5.2

724028

Kikumoto

PubMed abstract available

Tetrathionate-forming thiosulf ...

Acidithiobacillus ferrooxidans

Appl. Environ. Microbiol.

79

113-120

2013

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659545

Janiczek

PubMed abstract available

Use of immobilized cytochrome ...

Acidithiobacillus ferrooxidans, Acidithiobacillus ferrooxidans CCM 4253

J. Biotechnol.

117

293-298

2005

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656833

Muller

PubMed abstract available

Coupling of the pathway of sul ...

Acidianus ambivalens

Mol. Microbiol.

53

1147-1160

2004

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