BRENDA - Enzyme Database show
show all sequences of 1.8.5.2

Tetrathionate-forming thiosulfate dehydrogenase from the acidophilic, chemolithoautotrophic bacterium Acidithiobacillus ferrooxidans

Kikumoto, M.; Nogami, S.; Kanao, T.; Takada, J.; Kamimura, K.; Appl. Environ. Microbiol. 79, 113-120 (2013)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
Sodium sulfate
the purified enzyme requires 150 mM sodium sulfate to generate maximum activity
Acidithiobacillus ferrooxidans
sulfite
the addition of sulfite (2 mM) results in the activation of the enzyme at a magnitude of 1.6fold
Acidithiobacillus ferrooxidans
Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Acidithiobacillus ferrooxidans
Inhibitors
Inhibitors
Commentary
Organism
Structure
Sodium sulfate
60% reduction in enzyme activity is observed in the reaction mixture containing 200 mM sodium sulfate
Acidithiobacillus ferrooxidans
sulfite
almost complete inhibition in the presence of 10 mM sulfite
Acidithiobacillus ferrooxidans
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
8
-
thiosulfate
recombinant enzyme, at pH 2.5 and 50°C
Acidithiobacillus ferrooxidans
15
-
thiosulfate
native enzyme, at pH 2.5 and 70°C
Acidithiobacillus ferrooxidans
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
25000
-
x * 25000, SDS-PAGE
Acidithiobacillus ferrooxidans
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Acidithiobacillus ferrooxidans
-
-
-
Purification (Commentary)
Commentary
Organism
ammonium sulfate precipitation, CM-650M column chromatography, butyl-650M column chromatography, and TSKgel G3000 gel filtration
Acidithiobacillus ferrooxidans
Source Tissue
Source Tissue
Commentary
Organism
Textmining
culture condition:sulfur-grown cell
-
Acidithiobacillus ferrooxidans
-
culture condition:tetrathionate-grown cell
-
Acidithiobacillus ferrooxidans
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.05
-
unpurified recombinant enzyme, at pH 2.5 and 40°C
Acidithiobacillus ferrooxidans
3.16
-
recombinant enzyme after 63.2fold purification, at pH 2.5 and 40°C
Acidithiobacillus ferrooxidans
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
the enzyme reduces neither ubiquinone nor horse heart cytochrome c, which serves as an electron acceptor
724028
Acidithiobacillus ferrooxidans
?
-
-
-
-
thiosulfate + ferricyanide + H+
when the concentration of thiosulfate is lower than 4 mM, no enzyme activity is detected
724028
Acidithiobacillus ferrooxidans
tetrathionate + ferrocyanide
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 25000, SDS-PAGE
Acidithiobacillus ferrooxidans
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
50
-
recombinant enzyme
Acidithiobacillus ferrooxidans
70
-
native enzyme
Acidithiobacillus ferrooxidans
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.5
-
native and recombinant enzyme
Acidithiobacillus ferrooxidans
pH Range
pH Minimum
pH Maximum
Commentary
Organism
2
3
the native enzyme shows about 35% activity at pH 2.0 and 20% activity at pH 3.0, the recombinant enzyme shows about 80% activity at pH 2.0 and about 35% activity at pH 3.0
Acidithiobacillus ferrooxidans
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
does not contain heme
Acidithiobacillus ferrooxidans
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
Sodium sulfate
the purified enzyme requires 150 mM sodium sulfate to generate maximum activity
Acidithiobacillus ferrooxidans
sulfite
the addition of sulfite (2 mM) results in the activation of the enzyme at a magnitude of 1.6fold
Acidithiobacillus ferrooxidans
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Acidithiobacillus ferrooxidans
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
does not contain heme
Acidithiobacillus ferrooxidans
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Sodium sulfate
60% reduction in enzyme activity is observed in the reaction mixture containing 200 mM sodium sulfate
Acidithiobacillus ferrooxidans
sulfite
almost complete inhibition in the presence of 10 mM sulfite
Acidithiobacillus ferrooxidans
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
8
-
thiosulfate
recombinant enzyme, at pH 2.5 and 50°C
Acidithiobacillus ferrooxidans
15
-
thiosulfate
native enzyme, at pH 2.5 and 70°C
Acidithiobacillus ferrooxidans
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
25000
-
x * 25000, SDS-PAGE
Acidithiobacillus ferrooxidans
Purification (Commentary) (protein specific)
Commentary
Organism
ammonium sulfate precipitation, CM-650M column chromatography, butyl-650M column chromatography, and TSKgel G3000 gel filtration
Acidithiobacillus ferrooxidans
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
culture condition:sulfur-grown cell
-
Acidithiobacillus ferrooxidans
-
culture condition:tetrathionate-grown cell
-
Acidithiobacillus ferrooxidans
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.05
-
unpurified recombinant enzyme, at pH 2.5 and 40°C
Acidithiobacillus ferrooxidans
3.16
-
recombinant enzyme after 63.2fold purification, at pH 2.5 and 40°C
Acidithiobacillus ferrooxidans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
the enzyme reduces neither ubiquinone nor horse heart cytochrome c, which serves as an electron acceptor
724028
Acidithiobacillus ferrooxidans
?
-
-
-
-
thiosulfate + ferricyanide + H+
when the concentration of thiosulfate is lower than 4 mM, no enzyme activity is detected
724028
Acidithiobacillus ferrooxidans
tetrathionate + ferrocyanide
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 25000, SDS-PAGE
Acidithiobacillus ferrooxidans
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
50
-
recombinant enzyme
Acidithiobacillus ferrooxidans
70
-
native enzyme
Acidithiobacillus ferrooxidans
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.5
-
native and recombinant enzyme
Acidithiobacillus ferrooxidans
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
2
3
the native enzyme shows about 35% activity at pH 2.0 and 20% activity at pH 3.0, the recombinant enzyme shows about 80% activity at pH 2.0 and about 35% activity at pH 3.0
Acidithiobacillus ferrooxidans
Other publictions for EC 1.8.5.2
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
724028
Kikumoto
Tetrathionate-forming thiosulf ...
Acidithiobacillus ferrooxidans
Appl. Environ. Microbiol.
79
113-120
2013
2
-
1
-
-
-
2
2
-
-
1
-
-
4
-
-
1
-
-
2
2
-
2
1
2
-
-
-
1
1
-
1
-
-
-
2
-
1
1
-
-
-
-
2
-
2
-
-
1
-
-
-
-
1
-
2
2
-
2
1
2
-
-
-
1
1
-
-
-
-
-
-
-
-
659545
Janiczek
Use of immobilized cytochrome ...
Acidithiobacillus ferrooxidans, Acidithiobacillus ferrooxidans CCM 4253
J. Biotechnol.
117
293-298
2005
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
656833
Muller
Coupling of the pathway of sul ...
Acidianus ambivalens
Mol. Microbiol.
53
1147-1160
2004
-
-
-
-
-
-
8
4
1
-
5
2
-
5
-
2
1
-
-
-
4
-
8
1
1
1
-
1
2
2
-
1
1
-
-
-
-
-
1
-
-
-
-
8
1
4
1
-
5
2
-
-
2
1
-
-
4
-
8
1
1
1
-
1
2
2
-
-
-
-
-
-
-
-