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Information on EC 1.8.1.7 - glutathione-disulfide reductase and Organism(s) Nicotiana tabacum and UniProt Accession P80461

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IUBMB Comments
A dimeric flavoprotein (FAD); activity is dependent on a redox-active disulfide in each of the active centres.
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This record set is specific for:
Nicotiana tabacum
UNIPROT: P80461
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Word Map
The taxonomic range for the selected organisms is: Nicotiana tabacum
The enzyme appears in selected viruses and cellular organisms
Synonyms
glutathione reductase, gsh reductase, gssg reductase, glutathione disulfide reductase, ptgr2, gsr-1, glutathione-disulfide reductase, glutathione s-reductase, thioredoxin/glutathione reductase, trxr3, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glutathione reductase
-
EC 1.6.4.2
-
formerly
glutathione reductase
-
-
-
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glutathione reductase (NADPH)
-
-
-
-
glutathione S-reductase
-
-
-
-
GOR1
-
-
-
-
GOR2
-
-
-
-
GRase
-
-
-
-
GSH reductase
-
-
-
-
GSSG reductase
-
-
-
-
NADPH-glutathione reductase
-
-
-
-
NADPH-GSSG reductase
-
-
-
-
NADPH:oxidized-glutathione oxidoreductase
-
-
-
-
OBP29
-
-
-
-
reductase, glutathione
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
redox reaction
-
-
-
-
oxidation
-
-
-
-
reduction
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
glutathione:NADP+ oxidoreductase
A dimeric flavoprotein (FAD); activity is dependent on a redox-active disulfide in each of the active centres.
CAS REGISTRY NUMBER
COMMENTARY hide
9001-48-3
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
glutathione disulfide + NADPH + H+
glutathione + NADP+
show the reaction diagram
-
-
-
?
glutathione disulfide + NADPH + H+
glutathione + NADP+
show the reaction diagram
-
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
glutathione disulfide + NADPH + H+
glutathione + NADP+
show the reaction diagram
-
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
FAD
-
the enzyme contains one FAD per monomer
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
main localization
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
-
the enzyme plays an essential central role in cell defense against reactive oxygen metabolites. The enzyme plays an important role in plant protection against various forms of stress
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
GSHRP_TOBAC
557
0
60035
Swiss-Prot
Chloroplast (Reliability: 1)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
47000
one of two minor protein bands at 50000 and 47000 Da, SDS-PAGE
50000
one of two minor protein bands at 50000 and 47000 Da, SDS-PAGE
55000
major protein band, approximately 55000 Da, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
-
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Ni-NTA agarose resin column chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Nicotiana tabaccum cultivar Wisconsin 38
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
after treatment with methyl viologen for two weeks, there is a significant increase in the glutathione reductase activity in both wild type and transgenic plants and such an increase is greater in transgenic plants than in wild type plants
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Ding, S.; Lu, Q.; Zhang, Y.; Yang, Z.; Wen, X.; Zhang, L.; Lu, C.
Enhanced sensitivity to oxidative stress in transgenic tobacco plants with decreased glutathione reductase activity leads to a decrease in ascorbate pool and ascorbate redox state
Plant Mol. Biol.
69
577-592
2009
Nicotiana tabacum (P80461), Nicotiana tabacum
Manually annotated by BRENDA team
Gill, S.S.; Anjum, N.A.; Hasanuzzaman, M.; Gill, R.; Trivedi, D.K.; Ahmad, I.; Pereira, E.; Tuteja, N.
Glutathione and glutathione reductase: a boon in disguise for plant abiotic stress defense operations
Plant Physiol. Biochem.
70
204-212
2013
Arachis hypogaea, Beta vulgaris, Cajanus cajan, Citrullus lanatus, Cucumis sativus, Cucumis melo, Glycine max, Nicotiana tabacum, no activity in Drosophila melanogaster, Oryza sativa, Vigna radiata var. radiata, Pisum sativum, Spinacia oleracea, Triticum aestivum, Zea mays, Acorus calamus, Citrus trifoliata, no activity in Trypanosoma sp.
Manually annotated by BRENDA team