Information on EC 1.4.1.15 - lysine dehydrogenase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.4.1.15
-
RECOMMENDED NAME
GeneOntology No.
lysine dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
L-lysine + NAD+ = 1,2-didehydropiperidine-2-carboxylate + NH3 + NADH + H+
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Deamination
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oxidation
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redox reaction
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reduction
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SYSTEMATIC NAME
IUBMB Comments
L-lysine:NAD+ oxidoreductase (deaminating, cyclizing)
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CAS REGISTRY NUMBER
COMMENTARY hide
68073-29-0
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
Bacterium cadaveris strain IFO 3731
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Manually annotated by BRENDA team
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-
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Manually annotated by BRENDA team
strains ICR 0220, IFO 3310 and IFO 12059
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Manually annotated by BRENDA team
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-
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Manually annotated by BRENDA team
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-
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Manually annotated by BRENDA team
no activity in Achromobacter superficialis
-
-
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Manually annotated by BRENDA team
no activity in Achromobacter superficialis ICR 0890
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-
-
Manually annotated by BRENDA team
no activity in Aerobacter aerogenes
-
-
-
Manually annotated by BRENDA team
no activity in Aerobacter aerogenes IFO 3320
-
-
-
Manually annotated by BRENDA team
no activity in Arthrobacter tumescens
-
-
-
Manually annotated by BRENDA team
no activity in Arthrobacter tumescens IFO 12960
-
-
-
Manually annotated by BRENDA team
no activity in Bacillus cereus
-
-
-
Manually annotated by BRENDA team
no activity in Bacillus cereus IFO 3001
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-
-
Manually annotated by BRENDA team
no activity in Bacillus licheniformis
-
-
-
Manually annotated by BRENDA team
no activity in Bacillus licheniformis IFO 12200
-
-
-
Manually annotated by BRENDA team
no activity in Bacillus megaterium
-
-
-
Manually annotated by BRENDA team
no activity in Bacillus megaterium ICR 1340
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-
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Manually annotated by BRENDA team
no activity in Bacillus subtilis
-
-
-
Manually annotated by BRENDA team
no activity in Bacillus subtilis IFO 3037
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-
-
Manually annotated by BRENDA team
no activity in Bos taurus
liver homogenate
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-
Manually annotated by BRENDA team
no activity in Brevibacterium divaricatum
-
-
-
Manually annotated by BRENDA team
no activity in Brevibacterium divaricatum ICR 4100
-
-
-
Manually annotated by BRENDA team
no activity in Candida albicans
strains IFO 0197 and 0601
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-
Manually annotated by BRENDA team
no activity in Candida melibiosi
-
-
-
Manually annotated by BRENDA team
no activity in Candida melibiosi IFO 0454
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-
Manually annotated by BRENDA team
no activity in Candida tropicalis
-
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Manually annotated by BRENDA team
no activity in Candida tropicalis IFO 1401
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-
-
Manually annotated by BRENDA team
no activity in Corynebacterium glutamicum
strain ATCC 13032
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-
Manually annotated by BRENDA team
no activity in Cryptococcus albidus
-
-
-
Manually annotated by BRENDA team
no activity in Cryptococcus albidus IFO 0410
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-
-
Manually annotated by BRENDA team
no activity in Cyberlindnera petersonii
-
-
-
Manually annotated by BRENDA team
no activity in Cyberlindnera petersonii IFO 1372
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-
-
Manually annotated by BRENDA team
no activity in Cystobasidium minutum
-
-
-
Manually annotated by BRENDA team
no activity in Cystobasidium minutum IFO 0412
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-
-
Manually annotated by BRENDA team
no activity in Debaryomyces hansenii
-
-
-
Manually annotated by BRENDA team
no activity in Debaryomyces hansenii IFO 0794
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-
-
Manually annotated by BRENDA team
no activity in Endomycopsis burtonii
strains IFO 1196 and 0844
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-
Manually annotated by BRENDA team
no activity in Erwinia aroideae
-
-
-
Manually annotated by BRENDA team
no activity in Erwinia aroideae IFO 3830
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-
Manually annotated by BRENDA team
no activity in Escherichia coli
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-
-
Manually annotated by BRENDA team
no activity in Flavobacterium lutescens
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Manually annotated by BRENDA team
no activity in Flavobacterium lutescens IFO 3014
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-
Manually annotated by BRENDA team
no activity in Haemonchus contortus
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-
-
Manually annotated by BRENDA team
no activity in Hansenula anomala
strain 0118
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-
Manually annotated by BRENDA team
no activity in Hansenula anomala 118
strain 0118
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-
Manually annotated by BRENDA team
no activity in Hansenula beijerinckii
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-
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Manually annotated by BRENDA team
no activity in Hansenula beijerinckii IFO 0981
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Manually annotated by BRENDA team
no activity in Hansenula bimundalis
-
-
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Manually annotated by BRENDA team
no activity in Hansenula bimundalis IFO 1366
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-
Manually annotated by BRENDA team
no activity in Hansenula canadenis
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-
Manually annotated by BRENDA team
no activity in Hansenula canadenis IFO 0937
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Manually annotated by BRENDA team
no activity in Hansenula holstii
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Manually annotated by BRENDA team
no activity in Hansenula holstii IFO 0980
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Manually annotated by BRENDA team
no activity in Hansenula mrakii
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Manually annotated by BRENDA team
no activity in Hansenula mrakii IFO 0895
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-
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Manually annotated by BRENDA team
no activity in Hansenula saturnus
and strain IFO 0992
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-
Manually annotated by BRENDA team
no activity in Hansenula saturnus IFO 0125
and strain IFO 0992
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Manually annotated by BRENDA team
no activity in Kluyveromyces lactis
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Manually annotated by BRENDA team
no activity in Kluyveromyces lactis IFO 1090
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Manually annotated by BRENDA team
no activity in Oosporidium margaritiferum
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Manually annotated by BRENDA team
no activity in Oosporidium margaritiferum IFO 1208
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Manually annotated by BRENDA team
no activity in Pichia polymorpha
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Manually annotated by BRENDA team
no activity in Pichia polymorpha IFO 0195
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Manually annotated by BRENDA team
no activity in Proteus vulgaris
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Manually annotated by BRENDA team
no activity in Proteus vulgaris IFO 3167
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Manually annotated by BRENDA team
no activity in Pseudomonas aeruginosa
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Manually annotated by BRENDA team
no activity in Pseudomonas aeruginosa IFO 3080
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Manually annotated by BRENDA team
no activity in Pseudomonas aureofaciens
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Manually annotated by BRENDA team
no activity in Pseudomonas aureofaciens IFO 3521
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Manually annotated by BRENDA team
no activity in Pseudomonas dacunhae
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Manually annotated by BRENDA team
no activity in Pseudomonas dacunhae ICR 3180
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Manually annotated by BRENDA team
no activity in Pseudomonas fluorescens
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Manually annotated by BRENDA team
no activity in Pseudomonas fluorescens IFO 3081
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Manually annotated by BRENDA team
no activity in Pseudomonas marginalis
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Manually annotated by BRENDA team
no activity in Pseudomonas marginalis IFO 3925
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Manually annotated by BRENDA team
no activity in Pseudomonas ovalis
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Manually annotated by BRENDA team
no activity in Pseudomonas ovalis IFO 3738
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Manually annotated by BRENDA team
no activity in Rhodotorula glutinis
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Manually annotated by BRENDA team
no activity in Rhodotorula glutinis IFO 0898
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Manually annotated by BRENDA team
no activity in Rhodotorula lactosa
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Manually annotated by BRENDA team
no activity in Rhodotorula lactosa IFO 1006
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Manually annotated by BRENDA team
no activity in Rhodotorula marina
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Manually annotated by BRENDA team
no activity in Rhodotorula marina IFO 0879
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Manually annotated by BRENDA team
no activity in Rhodotorula minuta
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Manually annotated by BRENDA team
no activity in Rhodotorula minuta IFO 0387
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Manually annotated by BRENDA team
no activity in Rhodotorula rubra
strains IFO 0001, 0003, 0870 and 0900
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Manually annotated by BRENDA team
no activity in Sarcina subflava
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Manually annotated by BRENDA team
no activity in Sarcina subflava IFO 11992
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Manually annotated by BRENDA team
no activity in Serratia marcescens
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Manually annotated by BRENDA team
no activity in Serratia marcescens IFO 3046
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Manually annotated by BRENDA team
no activity in Staphylococcus aureus
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Manually annotated by BRENDA team
no activity in Staphylococcus aureus IFO 3060
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Manually annotated by BRENDA team
no activity in Teladorsagia circumcincta
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Manually annotated by BRENDA team
no activity in Torulopsis candida
strains IFO 0380 and 0768
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Manually annotated by BRENDA team
no activity in Trichosporon cutaneum
strains IFO 0174 and 1198
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Manually annotated by BRENDA team
no activity in Yarrowia lipolytica
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Manually annotated by BRENDA team
no activity in Yarrowia lipolytica IFO 1209
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
gene lysdh
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
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genomic organization and functional role of lysine catabolic pathways among prokaryotes, overview
physiological function
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the enzyme is part of the lysine-to alpha-aminoadipic-delta-semialdehyde-pathway, that has a broad evolutionary importance in osmotic stress resistance
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-hydroxylysine + NADP+
alpha-amino-gamma-hydroxyadipate-semialdehyde + NADPH
show the reaction diagram
5-hydroxylysine + NADP+
alpha-amino-delta-hydroxyadipate-semialdehyde + NADPH
show the reaction diagram
L-lysine + NAD+
1,2-didehydropiperidine-2-carboxylate + NH3 + NADH
show the reaction diagram
-
-
-
?
L-lysine + NAD+
1,2-didehydropiperidine-2-carboxylate + NH3 + NADH + H+
show the reaction diagram
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-
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r
L-lysine + NAD+ + H2O
1,2-didehydropiperidine-6-carboxylate + NH3 + NADH
show the reaction diagram
S-(2-aminoethyl)-L-cysteine + NADP+ + H2O
S-(2-oxoethyl)-L-cysteine + NADPH + NH3
show the reaction diagram
additional information
?
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LysDH directly converts L-lysine to alpha-aminoadipic-delta-semialdehyde
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
L-lysine + NAD+
1,2-didehydropiperidine-2-carboxylate + NH3 + NADH
show the reaction diagram
-
-
-
?
L-lysine + NAD+
1,2-didehydropiperidine-2-carboxylate + NH3 + NADH + H+
show the reaction diagram
-
-
-
-
r
L-lysine + NAD+ + H2O
1,2-didehydropiperidine-6-carboxylate + NH3 + NADH
show the reaction diagram
-
epsilon amino group is oxidatively deaminated to alpha-aminoadipate delta-semialdehyde, which is spontaneously converted into 1,2-didehydropiperidine-6-carboxylate, first step of lysine degradation
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5,5-dithiobis(2-nitrobenzoate)
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0.5 mM, 70% inhibition, 40% inhibition after preincubation with 10 mM L-lysine, 98.5% inhibition after preincubation with 1 mM NAD+
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5-Aminovalerate
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5 mM, 1.65fold activation
6-aminocaproate
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5 mM, 2.32fold activation
7-aminoheptanoate
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5 mM, 2.3fold activation
8-aminooctanoate
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5 mM, 2.26fold activation
D-Lysine
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5 mM, 1.58fold activation
D-phenylalanine
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5 mM, 1.23fold activation
D-tryptophan
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5 mM, 2.01fold activation
DL-alpha-hydroxy-n-caproate
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5 mM, 1.85fold activation
DL-Homolysine
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5 mM, 1.8fold activation
L-isoleucine
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5 mM, 1.72fold activation
L-leucine
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5 mM, 1.69fold activation
L-lysine
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5 mM, 2.29fold activation
L-methionine
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5 mM, 1.54fold activation
L-norleucine
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5 mM, 2.05fold activation
L-norvaline
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5 mM, 1.71fold activation
L-phenylalanine
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5 mM, 2.09fold activation
L-phenylglycine
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5 mM, 1.74fold activation
L-Phenyllactate
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5 mM, 2.25fold activation
L-tryptophan
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5 mM, 1.24fold activation
L-tyrosine
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5 mM, 1.41fold activation
L-valine
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5 mM, 1.47fold activation
S-(2-aminoethyl)-L-cysteine
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5 mM, 2.32fold activation
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3.13
3-acetylpyridine-NAD+
-
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1.85
deamino-NAD+
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00004
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enzyme activity in crude extracts
0.000059
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enzyme activity in crude extracts
0.000069
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enzyme activity in crude extracts
0.0001
-
enzyme activity in crude extracts
0.00017
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enzyme activity in crude extracts, strain IFO 3319
0.00021
-
enzyme activity in crude extracts
0.00026
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enzyme activity in crude extracts
0.00067
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enzyme activity in crude extracts
0.00069
-
enzyme activity in crude extracts
0.0016
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enzyme activity in crude extracts
0.0029
-
enzyme activity in crude extracts
0.0052
-
enzyme activity in crude extracts, strain ICR 0220
0.0144
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activity in cells grown on 5 mM lysine
0.0392
-
enzyme activity in crude extracts, strain IFO 12059
0.0696
-
enzyme activity in crude extracts, strain IFO 3058
0.0739
-
enzyme activity in crude extracts, strain ICR 1600
additional information
-
0.874 micromol/min*l homogenate, 3 months old baby girl with congenital lysine intolerance, in the absence of cysteine; 11.96 micromol/min*l homogenate, in the presence of cysteine; 2.70 micromol/min*l homogenate, 3 months old baby girl with congenital lysine intolerance, in the presence of cysteine; 6.24 micromol/min*l homogenate, in the absence of cysteine
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
9
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oxidative deamination of S-(beta-aminoethyl)-cysteine, 5- and 4-hydroxylysine
9.3
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oxidative deamination of lysine
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
32
-
with S-(2-aminoethyl)-cysteine and 5-hydroxylysine
37
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with lysine and 4-hydroxylysine
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30 - 50
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in the absence of lysine, approx. 50% activity after 10 min at 40°C
30 - 60
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in the presence of 5 mM L-lysine, approx. 50% activity after 10 min at 55°C
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
gene lysdh is not upregulated under salt stress, in contrast to several other enzymes from the lysine-to-alpha-aminoadipic-delta-semialdehyde pathways, but is upregulated by addition of exogenous lysine
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
analysis
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amperometric biosensor for L-lysine based on immobilized enzyme