BRENDA - Enzyme Database show
show all sequences of 1.4.1.15

NAD+-dependent lysine dehydrogenase from a plant-pathogenic bacterium, Agrobacterium tumefaciens

Misono, H.; Vitamins (Kyoto) 65, 1-12 (1991)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
5-Aminovalerate
5 mM, 1.65fold activation
Agrobacterium tumefaciens
6-aminocaproate
5 mM, 2.32fold activation
Agrobacterium tumefaciens
7-aminoheptanoate
5 mM, 2.3fold activation
Agrobacterium tumefaciens
8-aminooctanoate
5 mM, 2.26fold activation
Agrobacterium tumefaciens
D-Lysine
5 mM, 1.58fold activation
Agrobacterium tumefaciens
D-phenylalanine
5 mM, 1.23fold activation
Agrobacterium tumefaciens
D-tryptophan
5 mM, 2.01fold activation
Agrobacterium tumefaciens
DL-alpha-hydroxy-n-caproate
5 mM, 1.85fold activation
Agrobacterium tumefaciens
DL-Homolysine
5 mM, 1.8fold activation
Agrobacterium tumefaciens
L-isoleucine
5 mM, 1.72fold activation
Agrobacterium tumefaciens
L-leucine
5 mM, 1.69fold activation
Agrobacterium tumefaciens
L-lysine
5 mM, 2.29fold activation
Agrobacterium tumefaciens
L-methionine
5 mM, 1.54fold activation
Agrobacterium tumefaciens
L-norleucine
5 mM, 2.05fold activation
Agrobacterium tumefaciens
L-norvaline
5 mM, 1.71fold activation
Agrobacterium tumefaciens
L-phenylalanine
5 mM, 2.09fold activation
Agrobacterium tumefaciens
L-phenylglycine
5 mM, 1.74fold activation
Agrobacterium tumefaciens
L-Phenyllactate
5 mM, 2.25fold activation
Agrobacterium tumefaciens
L-tryptophan
5 mM, 1.24fold activation
Agrobacterium tumefaciens
L-tyrosine
5 mM, 1.41fold activation
Agrobacterium tumefaciens
L-valine
5 mM, 1.47fold activation
Agrobacterium tumefaciens
S-(2-aminoethyl)-L-cysteine
5 mM, 2.32fold activation
Agrobacterium tumefaciens
Inhibitors
Inhibitors
Commentary
Organism
Structure
5,5-dithiobis(2-nitrobenzoate)
0.5 mM, 70% inhibition, 40% inhibition after preincubation with 10 mM L-lysine, 98.5% inhibition after preincubation with 1 mM NAD+
Agrobacterium tumefaciens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.06
-
NAD+
-
Agrobacterium tumefaciens
1.85
-
deamino-NAD+
-
Agrobacterium tumefaciens
3.13
-
3-acetylpyridine-NAD+
-
Agrobacterium tumefaciens
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Agrobacterium tumefaciens
-
-
-
Purification (Commentary)
Commentary
Organism
-
Agrobacterium tumefaciens
Reaction
Reaction
Commentary
Organism
L-lysine + NAD+ = 1,2-didehydropiperidine-2-carboxylate + NH3 + NADH + H+
the pro-R hydrogen at the prochiral C-6 carbon of L-lysine is specifically abstracted and transferred to the pro-R position at C-4 of NAD+
Agrobacterium tumefaciens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-lysine + NAD+ + H2O
-
391499
Agrobacterium tumefaciens
1,2-didehydropiperidine-6-carboxylate + NH3 + NADH
-
391499
Agrobacterium tumefaciens
?
Subunits
Subunits
Commentary
Organism
dimer
-
Agrobacterium tumefaciens
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
30
50
in the absence of lysine, approx. 50% activity after 10 min at 40°C
Agrobacterium tumefaciens
30
60
in the presence of 5 mM L-lysine, approx. 50% activity after 10 min at 55°C
Agrobacterium tumefaciens
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
NADP+ can not replace NAD+
Agrobacterium tumefaciens
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
5-Aminovalerate
5 mM, 1.65fold activation
Agrobacterium tumefaciens
6-aminocaproate
5 mM, 2.32fold activation
Agrobacterium tumefaciens
7-aminoheptanoate
5 mM, 2.3fold activation
Agrobacterium tumefaciens
8-aminooctanoate
5 mM, 2.26fold activation
Agrobacterium tumefaciens
D-Lysine
5 mM, 1.58fold activation
Agrobacterium tumefaciens
D-phenylalanine
5 mM, 1.23fold activation
Agrobacterium tumefaciens
D-tryptophan
5 mM, 2.01fold activation
Agrobacterium tumefaciens
DL-alpha-hydroxy-n-caproate
5 mM, 1.85fold activation
Agrobacterium tumefaciens
DL-Homolysine
5 mM, 1.8fold activation
Agrobacterium tumefaciens
L-isoleucine
5 mM, 1.72fold activation
Agrobacterium tumefaciens
L-leucine
5 mM, 1.69fold activation
Agrobacterium tumefaciens
L-lysine
5 mM, 2.29fold activation
Agrobacterium tumefaciens
L-methionine
5 mM, 1.54fold activation
Agrobacterium tumefaciens
L-norleucine
5 mM, 2.05fold activation
Agrobacterium tumefaciens
L-norvaline
5 mM, 1.71fold activation
Agrobacterium tumefaciens
L-phenylalanine
5 mM, 2.09fold activation
Agrobacterium tumefaciens
L-phenylglycine
5 mM, 1.74fold activation
Agrobacterium tumefaciens
L-Phenyllactate
5 mM, 2.25fold activation
Agrobacterium tumefaciens
L-tryptophan
5 mM, 1.24fold activation
Agrobacterium tumefaciens
L-tyrosine
5 mM, 1.41fold activation
Agrobacterium tumefaciens
L-valine
5 mM, 1.47fold activation
Agrobacterium tumefaciens
S-(2-aminoethyl)-L-cysteine
5 mM, 2.32fold activation
Agrobacterium tumefaciens
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
NADP+ can not replace NAD+
Agrobacterium tumefaciens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
5,5-dithiobis(2-nitrobenzoate)
0.5 mM, 70% inhibition, 40% inhibition after preincubation with 10 mM L-lysine, 98.5% inhibition after preincubation with 1 mM NAD+
Agrobacterium tumefaciens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.06
-
NAD+
-
Agrobacterium tumefaciens
1.85
-
deamino-NAD+
-
Agrobacterium tumefaciens
3.13
-
3-acetylpyridine-NAD+
-
Agrobacterium tumefaciens
Purification (Commentary) (protein specific)
Commentary
Organism
-
Agrobacterium tumefaciens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-lysine + NAD+ + H2O
-
391499
Agrobacterium tumefaciens
1,2-didehydropiperidine-6-carboxylate + NH3 + NADH
-
391499
Agrobacterium tumefaciens
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
-
Agrobacterium tumefaciens
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
30
50
in the absence of lysine, approx. 50% activity after 10 min at 40°C
Agrobacterium tumefaciens
30
60
in the presence of 5 mM L-lysine, approx. 50% activity after 10 min at 55°C
Agrobacterium tumefaciens
Other publictions for EC 1.4.1.15
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
725167
Neshich
Genome-wide analysis of lysine ...
Ruegeria pomeroyi
ISME J.
7
240-2410
2013
-
-
-
-
-
-
-
-
-
-
-
1
-
5
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
1
2
2
1
-
-
724927
Umair
Lysine catabolism in Haemonchu ...
no activity in Haemonchus contortus, no activity in Teladorsagia circumcincta
Exp. Parasitol.
131
101-106
2012
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
391500
Hammer
Enzymic production of alpha-am ...
Candida albicans, Candida albicans SBUG 182
Zentralbl. Mikrobiol.
147
65-70
1992
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
8
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
8
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
391501
Dempsey
A lysine dehydrogenase-based e ...
Agrobacterium tumefaciens
Biosens. Bioelectron.
7
323-327
1992
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
391499
Misono
-
NAD+-dependent lysine dehydrog ...
Agrobacterium tumefaciens
Vitamins (Kyoto)
65
1-12
1991
22
-
-
-
-
-
1
3
-
-
-
-
-
1
-
-
1
1
-
-
-
-
1
1
-
-
2
-
-
-
-
1
-
-
-
22
-
-
1
-
-
-
-
1
-
3
-
-
-
-
-
-
-
1
-
-
-
-
1
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
391498
Misono
-
Distribution and physiological ...
Agrobacterium tumefaciens, Alcaligenes faecalis, Alcaligenes faecalis IFO 3160, Corynebacterium ammoniagenes, Corynebacterium ammoniagenes IFO 12071, Corynebacterium pseudodiphtheriticum, Corynebacterium pseudodiphtheriticum ICR 2210, Hafnia alvei, Klebsiella aerogenes, Kocuria rosea, Kocuria rosea IFO 3764, Lysinibacillus sphaericus, Lysinibacillus sphaericus IFO 3525, Micrococcus flavus, Micrococcus flavus ICR 1820, Morganella morganii, Morganella morganii IFO 3848, no activity in Achromobacter superficialis, no activity in Achromobacter superficialis ICR 0890, no activity in Aerobacter aerogenes, no activity in Aerobacter aerogenes IFO 3320, no activity in Arthrobacter tumescens, no activity in Arthrobacter tumescens IFO 12960, no activity in Bacillus cereus, no activity in Bacillus cereus IFO 3001, no activity in Bacillus licheniformis, no activity in Bacillus licheniformis IFO 12200, no activity in Bacillus megaterium, no activity in Bacillus megaterium ICR 1340, no activity in Bacillus subtilis, no activity in Bacillus subtilis IFO 3037, no activity in Bos taurus, no activity in Brevibacterium divaricatum, no activity in Brevibacterium divaricatum ICR 4100, no activity in Candida albicans, no activity in Candida melibiosi, no activity in Candida melibiosi IFO 0454, no activity in Candida tropicalis, no activity in Candida tropicalis IFO 1401, no activity in Corynebacterium glutamicum, no activity in Cryptococcus albidus, no activity in Cryptococcus albidus IFO 0410, no activity in Cyberlindnera petersonii, no activity in Cyberlindnera petersonii IFO 1372, no activity in Cystobasidium minutum, no activity in Cystobasidium minutum IFO 0412, no activity in Debaryomyces hansenii, no activity in Debaryomyces hansenii IFO 0794, no activity in Endomycopsis burtonii, no activity in Erwinia aroideae, no activity in Erwinia aroideae IFO 3830, no activity in Escherichia coli, no activity in Flavobacterium lutescens, no activity in Flavobacterium lutescens IFO 3014, no activity in Hansenula anomala, no activity in Hansenula anomala 118, no activity in Hansenula beijerinckii, no activity in Hansenula beijerinckii IFO 0981, no activity in Hansenula bimundalis, no activity in Hansenula bimundalis IFO 1366, no activity in Hansenula canadensis, no activity in Hansenula canadensis IFO 0937, no activity in Hansenula holstii, no activity in Hansenula holstii IFO 0980, no activity in Hansenula mrakii, no activity in Hansenula mrakii IFO 0895, no activity in Hansenula saturnus, no activity in Hansenula saturnus IFO 0125, no activity in Kluyveromyces lactis, no activity in Kluyveromyces lactis IFO 1090, no activity in Oosporidium margaritiferum, no activity in Oosporidium margaritiferum IFO 1208, no activity in Pichia polymorpha, no activity in Pichia polymorpha IFO 0195, no activity in Proteus vulgaris, no activity in Proteus vulgaris IFO 3167, no activity in Pseudomonas aeruginosa, no activity in Pseudomonas aeruginosa IFO 3080, no activity in Pseudomonas aureofaciens, no activity in Pseudomonas aureofaciens IFO 3521, no activity in Pseudomonas dacunhae, no activity in Pseudomonas dacunhae ICR 3180, no activity in Pseudomonas fluorescens, no activity in Pseudomonas fluorescens IFO 3081, no activity in Pseudomonas marginalis, no activity in Pseudomonas marginalis IFO 3925, no activity in Pseudomonas ovalis, no activity in Pseudomonas ovalis IFO 3738, no activity in Rhodotorula glutinis, no activity in Rhodotorula glutinis IFO 0898, no activity in Rhodotorula lactosa, no activity in Rhodotorula lactosa IFO 1006, no activity in Rhodotorula marina, no activity in Rhodotorula marina IFO 0879, no activity in Rhodotorula minuta, no activity in Rhodotorula minuta IFO 0387, no activity in Rhodotorula rubra, no activity in Sarcina subflava, no activity in Sarcina subflava IFO 11992, no activity in Serratia marcescens, no activity in Serratia marcescens IFO 3046, no activity in Staphylococcus aureus, no activity in Staphylococcus aureus IFO 3060, no activity in Torulopsis candida, no activity in Trichosporon cutaneum, no activity in Yarrowia lipolytica, no activity in Yarrowia lipolytica IFO 1209, Pseudomonas fragi, Pseudomonas fragi IFO 3458, Sinomonas atrocyanea, Sinomonas atrocyanea IFO 12670
Agric. Biol. Chem.
47
631-633
1983
-
-
-
-
-
-
-
-
-
-
-
-
-
117
-
-
-
-
-
-
15
-
21
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
15
-
21
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
391497
Misono
Occurrence of L-lysine epsilon ...
Agrobacterium tumefaciens
J. Bacteriol.
150
398-401
1982
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
391496
Buergi
L-Lysine dehydrogenase deficie ...
Homo sapiens
Nature
211
854-855
1966
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-