HOME
login
history
all enzymes
BRENDA home
History
Information on EC 1.3.1.31 - 2-enoate reductase
for references in articles please use BRENDA:EC1.3.1.31Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
1.3.1.31 2-enoate reductaseIUBMB Comments
An iron-sulfur-flavoprotein (FAD). Acts (in the reverse direction) on a wide range of alkyl and aryl alphabeta-unsaturated carboxylate ions; but-2-enoate was the best substrate tested.
Specify your search results
Word Map
- 1.3.1.31
-
alpha,beta-unsaturated
- tyrobutyricum
-
spec
-
bioreduction
- kluyveri
-
beta-unsaturated
- methylviologen
The enzyme appears in viruses and cellular organisms
Synonyms
enoate reductase, yellow enzyme, lacer, 2-enoate reductase, nadph dehydrogenase 1, achr-oye4, achr-oye3, ered xenobiotic reductase b, yers-er, sye-4, 
more
topPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
Achr-OYE4
enoate reductase
ERED
old yellow enzyme
XenA
xenobiotic reductase A showing enoate reductase activity toward 2-cyclohexen-1-one
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
butanoate + NAD+ = but-2-enoate + NADH + H+
-
-
-
-
butanoate + NAD+ = but-2-enoate + NADH + H+
ping-pong mechanism in aqueous solution
-
butanoate + NAD+ = but-2-enoate + NADH + H+
enzyme kinetics in reversed micelles
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PATHWAY SOURCE
PATHWAYS
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYSTEMATIC NAME
IUBMB Comments
butanoate:NAD+ DELTA2-oxidoreductase
An iron-sulfur-flavoprotein (FAD). Acts (in the reverse direction) on a wide range of alkyl and aryl alphabeta-unsaturated carboxylate ions; but-2-enoate was the best substrate tested.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CAS REGISTRY NUMBER
COMMENTARY
70712-51-5
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(+)-carvone + NADPH
?
-
biotransformation in the presence of a NADP+/G6P/G6PDH cofactor recycling system, conversion: 100%, ee: 97%
-
?
(-)-carvone + NADPH
?
-
biotransformation in the presence of a NADP+/G6P/G6PDH cofactor recycling system, conversion: 100%, ee: 95%
-
?
(1-nitroprop-1-en-2-yl)benzene + NADP+
[(2R)-1-nitropropan-2-yl]benzene + NADPH + H+
-
-
product of isoform OPR1
?
(1-nitroprop-1-en-2-yl)benzene + NADP+
[(2S)-1-nitropropan-2-yl]benzene + NADPH + H+
-
-
product of isoform OPR3
?
(1-nitroprop-1-en-2-yl)benzene + NADP+
[(2S)-2-methyl-3-nitropropyl]benzene + NADPH + H+
-
-
-
?
(2E)-2-benzylidenecyclohexanone + NADPH
?
-
biotransformation in the presence of a NADP+/G6P/G6PDH cofactor recycling system, conversion: 92%, ee: 51%
-
?
(2E)-2-benzylidenecyclopentanone + NADPH
?
-
biotransformation in the presence of a NADP+/G6P/G6PDH cofactor recycling system, conversion: 4%, ee: racemate
-
?
(2E)-2-cyano-3-phenylprop-2-enoic acid + NADPH
2-cyano-3-phenylpropanoic acid + NADP+
conversion rate measured using HPLC after 48 h. Conversion: 83%, ee: racemic
-
?
(2E)-2-methyl-3-phenylacrylic acid + electron donor
2-methyl-3-phenylpropanoic acid + electron acceptor
-
-
-
?
(2E)-2-methyl-3-phenylbut-2-enoic acid + electron donor
2-methyl-3-phenylbutanoic acid + electron acceptor
-
-
-
?
(2E)-2-methylbut-2-enedioic acid + electron donor
2-methylbutanedioic aicd + electron acceptor
-
-
-
?
(2E)-2-methylpent-2-enal + NADH + H+
(2R)-2-methylpentanal + NAD+
-
-
-
?
(2E)-3,7-dimethylocta-2,6-dienal + NADH + H+
3,7-dimethyloct-6-enal + NAD+
-
-
-
?
(2E)-3-phenylacrylic acid + electron donor
3-phenylpropanoic acid + electron acceptor
-
-
-
?
(2E)-4-methoxy-2-methyl-4-oxobut-2-enoic acid + electron donor
4-methoxy-2-methyl-4-oxobutanoic acid + electron acceptor
-
-
-
?
(2E)-4-methoxy-3-methyl-4-oxobut-2-enoic acid + electron donor
4-methoxy-3-methyl-4-oxobutanoic acid + electron acceptor
-
-
-
?
(2E,4E)-2,3,5,8-tetramethylnona-2,4,7-trienoic acid + electron donor
? + electron acceptor
-
-
-
?
(2E,4E)-2,3-dimethylhexa-2,4-dienoic acid + electron donor
? + electron acceptor
-
-
-
?
(2Z)-3-(4-chlorophenyl)-3-cyanoprop-2-enoic acid + NADPH
(R)-3-(4-chlorophenyl)-3-cyano-propanoic acid + NADP+
conversion rate measured using HPLC after 48 h. Conversion: 80%, ee: 94% (R)
-
?
(5R)-2-methyl-5-(prop-1-en-2-yl)cyclohex-2-en-1-one + NADH + H+
(2R,5R)-2-methyl-5-(prop-1-en-2-yl)cyclohexan-1-one + NAD+
-
-
-
?
(5S)-2-methyl-5-(prop-1-en-2-yl)cyclohex-2-en-1-one + NADH + H+
(2R,5S)-2-methyl-5-(prop-1-en-2-yl)cyclohexan-1-one + NAD+
-
-
-
?
(E)-(2-nitroprop-1-en-1-yl)benzene + NADP+
[(3S)-3-nitrobutyl]benzene + NADPH + H+
-
-
-
?
(E)-2,5-dimethoxycinnamate + NADH
3-(2,5-dimethoxyphenyl)propionate + NAD+
-
-
-
?
(E)-2-hexen-1-al + NADH + H+
hexanal + NAD+
-
relative activity: 100%
-
?
(E)-2-methyl-2-butenal + NADH + H+
2-methylbutanal + NADH
-
relative activity: 59.2%
-
?
(E)-2-methyl-2-butenoate + 4,5-dihydroxyanthraquinone-2-carboxylic acid
2-methylbutanoate + ?
-
-
-
?
(E)-2-methyl-3-phenyl-2-propenoate + reduced methyl viologen
(R)-2-methyl-3-phenylpropanoate + methyl viologen
-
-
-
?
(E)-2-octenal + NADH + H+
octanal + NAD+
-
relative activity: 75.4%
-
?
(E)-2-oxo-4-phenyl-3-butenoate + NADH
2-oxo-4-phenyl-3-butanoate + NAD+
-
18% of the activity with butenoate
-
?
(E)-2-pentenal + NADH + H+
2-pentanone + NAD+
-
relative activity: 45%
-
?
(E)-3-methyl-2-oxo-4-phenyl-3-butenoate + NADH
3-methyl-2-oxo-4-phenyl-3-butenoate + NAD+
-
27% of the activity with butenoate
-
?
(E)-3-nonen-2-one + NADH + H+
nonan-2-one + NAD+
-
relative activity: 36.6%
-
?
(E)-3-nonen-2-one + NADPH
nonan-2-one + NADP+
-
relative activity: 61.6%
-
?
(E)-4-(2,4-dimethoxyphenyl)but-3-en-2-one + NAD+
4-(2,4-dimethoxyphenyl)butan-2-one + NADH + H+
(E)-4-(4'-isopropylphenyl)but-3-en-2-one + NAD+
4-(4-isopropylphenyl)butan-2-one + NADH + H+
(E)-4-(benzo[1,3]dioxol-5-yl)but-3-en-2-one + NAD+
4-(1,3-benzodioxol-5-yl)butan-2-one + NADH + H+
(E)-ethyl 2-methyl-4-oxopent-2-enoate + NADP+
ethyl (2R)-2-methyl-4-oxopentanoate + NADPH + H+
-
-
-
?
(Z)-2-bromocinnamate + NADH
3-(2-bromophenyl)propionate + NAD+
-
-
-
?
(Z)-2-chloro-3-(p-chlorophenyl)acrylate + NADH
2-chloro-3-(4-chlorophenyl)propionate + NAD+
-
-
-
?
(Z)-3-cyano-3-phenyl-propenoic acid + NADPH + H+
(R)-3-cyano-3-phenylpropanoic acid + NADP+
highest enantiomeric excess of 98% ee is achieved at a glucose concentration of 1% (w/v) with a 47% conversion for an 18 h reaction. Concentration of 2-propanol also affects the activity and selectivity of the reaction, and a concentration of 5% (v/v) results in the best substrate conversion and ee value of product. Conversion rate measured using HPLC after 48 h. Conversion: 80%, ee: 98% (R)
-
?
1,3-dimethyl-1H-pyrrole-2,5-dione + NADPH
?
-
biotransformation in the presence of a NADP+/G6P/G6PDH cofactor recycling system, conversion: 99%, ee: 99%
-
?
1-(cyclohex-1-en-1-yl)ethanone + NADPH
?
-
biotransformation in the presence of a NADP+/G6P/G6PDH cofactor recycling system, conversion: 71%, ee: not applicable
-
?
1-(cyclopent-1-en-1-yl)ethanone + NADPH
?
-
biotransformation in the presence of a NADP+/G6P/G6PDH cofactor recycling system, conversion: 30%, ee: not applicable
-
?
1-(prop-2-en-1-yl)-1H-pyrrole-2,5-dione + NADPH
?
-
biotransformation in the presence of a NADP+/G6P/G6PDH cofactor recycling system, conversion: 99%, ee: not applicable
-
?
1-benzyl-1H-pyrrole-2,5-dione + NADH + H+
1-benzylpyrrolidine-2,5-dione + NAD+
-
-
-
?
1-benzyl-3-methyl-1H-pyrrole-2,5-dione + NADH + H+
(3R)-1-benzyl-3-methylpyrrolidine-2,5-dione + NAD+
-
-
-
?
1-butyl-3-methyl-1H-pyrrole-2,5-dione + NADPH
?
-
biotransformation in the presence of a NADP+/G6P/G6PDH cofactor recycling system, conversion: 99%, ee: 99%
-
?
1-ethyl-3-methyl-1H-pyrrole-2,5-dione + NADPH
?
-
biotransformation in the presence of a NADP+/G6P/G6PDH cofactor recycling system, conversion: 99%, ee: 99%
-
?
12-phenyldodec-2-enoic acid + electron donor
12-phenyldodecanoic acid + electron acceptor
-
-
-
?
2,3-dimethylmaleimide + NADH
trans-dimethylsuccinimide + NAD+
-
-
-
?
2,6,6-trimethyl-2-cyclohexen-1,4-dione + NADPH
?
-
relative activity: 103%
-
?
2,6,6-trimethylcyclohex-2-ene-1,4-dione + NADH + H+
(6R)-2,2,6-trimethylcyclohexane-1,4-dione + NAD+
-
-
-
?
2-cyclohexen-1-one + NADH + H+
cyclohexanone + NAD+
-
relative activity: 830%
-
?
2-hydroxycyclopent-2-en-1-one + NADH + H+
(2S)-2-hydroxycyclopentan-1-one + NAD+
-
-
-
?
2-isopropyl-5-methyl-2-hexenal + NADH + H+
2-isopropyl-5-methylhexanal + NAD+
-
relative activity: 15.5%
-
?
2-methyl-2-cyclopenten-1-one + NADH + H+
?
-
relative activity: 62.7%
-
?
2-methylcyclopent-2-enone + NADPH
?
-
biotransformation in the presence of a NADP+/G6P/G6PDH cofactor recycling system, conversion: 92%, ee: 52%
-
?
3-methyl-1-(prop-2-en-1-yl)-1H-pyrrole-2,5-dione + NADPH
?
-
biotransformation in the presence of a NADP+/G6P/G6PDH cofactor recycling system, conversion: 99%, ee: 99%
-
?
3-methyl-1-phenyl-1H-pyrrole-2,5-dione + NADPH
(R)-N-phenyl-2-methylsuccinimide + NADP+
conversion rate measured using HPLC after 48 h. Conversion: 99%, ee: 99% (R)
-
?
3-methyl-1-propyl-1H-pyrrole-2,5-dione + NADPH
?
-
biotransformation in the presence of a NADP+/G6P/G6PDH cofactor recycling system, conversion: 99%, ee: 99%
-
?
4-(1,3-benzodioxol-5-yl)butan-2-one + NAD+
(S)-4-(1,3-benzodioxol-5-yl)butan-2-ol + NADH + H+
-
-
-
?
4-coumaric acid + NAD+
3-(4-hydroxyphenyl)propionic acid + NADH + H+
-
-
-
?
5-benzylidenethiazolidine-2,4-dione + NADH
(5R)-5-benzyl-1,3-thiazolidine-2,4-dione + NAD+
butenoate + reduced methyl viologen
butanoate + methyl viologen
-
-
-
?
diethyl (2Z)-2-methylbut-2-enedioate + NADPH
?
-
biotransformation in the presence of a NADP+/G6P/G6PDH cofactor recycling system, conversion: 99%, ee: 99%
-
?
dimethyl (2E)-2-methylbut-2-enedioate + NAD(P)H
dimethyl (2R)-2-methylbutanedioate + dimethyl (2S)-2-methylbutanedioate + NAD(P)+
dimethyl (2Z)-2-methylbut-2-enedioate + NAD(P)H
dimethyl (2R)-2-methylbutanedioate + NAD(P)+
dimethyl (2Z)-2-methylbut-2-enedioate + NADPH
?
-
biotransformation in the presence of a NADP+/G6P/G6PDH cofactor recycling system, conversion: 99%, ee: 98%
-
?
dimethyl 2-methylidenebutanedioate + NADPH
?
-
biotransformation in the presence of a NADP+/G6P/G6PDH cofactor recycling system, conversion: 19%, ee: 90%
-
?
ethyl (2Z)-3-nitro-2-phenylprop-2-enoate + NADPH
ethyl (2R)-3-nitro-2-phenylpropanoate + NADP+
conversion rate measured using HPLC after 48 h. Conversion: 40%, ee: 45% (R)
-
?
hexa-2,4-dienoic acid + electron donor
? + electron acceptor
-
-
-
?
methyl 2-phenylacrylate + NADP+
methyl (2R)-2-methyl-3-phenylpropanoate + NADPH + H+
-
-
-
?
oct-2-en-4-ynoic acid + electron donor
? + electron acceptor
-
-
-
?
[(1E)-1-nitroprop-1-en-2-yl]benzene + NADPH
(S)-1-nitro-2-phenylpropane + NADP+
conversion rate measured using HPLC after 48 h. Conversion: 87%, ee: 97% (S)
-
?
[(1E)-2-nitroprop-1-en-1-yl]benzene + NADPH
1-phenyl-2-nitropropane + NADP+
conversion rate measured using HPLC after 48 h. Conversion: 60%, ee: racemic
-
?
(+)-costunolide + NADPH
11(S),13-dihydrocostunolide + NADP+
-
part of the metabolization of (+)-costunolide to leucodin, overview
-
?
(E)-2-methyl-2-butenoate + NADH
2-methylbutanoate + NAD+
-
-
-
?
(E)-2-methyl-2-butenoate + NADH
2-methylbutanoate + NAD+
-
-
-
?
(E)-2-methyl-2-butenoate + NADH
2-methylbutanoate + NAD+
-
-
-
?
(E)-2-methylbutenoate + NADH
(R)-2-methylbutanoate + NAD+
-
-
-
?
(E)-2-methylbutenoate + NADH
(R)-2-methylbutanoate + NAD+
-
-
?
(E)-2-methylbutenoate + NADH
(R)-2-methylbutanoate + NAD+
-
-
?
(E)-2-methylbutenoate + reduced methylviologen
(R)-2-methylbutanoate + methyl viologen
-
-
-
?
(E)-2-methylbutenoate + reduced methylviologen
(R)-2-methylbutanoate + methyl viologen
-
-
-
?
(E)-4-(2,4-dimethoxyphenyl)but-3-en-2-one + NAD+
4-(2,4-dimethoxyphenyl)butan-2-one + NADH + H+
-
-
-
?
(E)-4-(2,4-dimethoxyphenyl)but-3-en-2-one + NAD+
4-(2,4-dimethoxyphenyl)butan-2-one + NADH + H+
-
-
-
?
(E)-4-(4'-isopropylphenyl)but-3-en-2-one + NAD+
4-(4-isopropylphenyl)butan-2-one + NADH + H+
-
-
-
?
(E)-4-(4'-isopropylphenyl)but-3-en-2-one + NAD+
4-(4-isopropylphenyl)butan-2-one + NADH + H+
-
-
-
?
(E)-4-(4'-methoxyphenyl)but-3-en-2-one + NAD+
4-(4-methoxyphenyl)butan-2-one + NADH + H+
-
-
-
?
(E)-4-(4'-methoxyphenyl)but-3-en-2-one + NAD+
4-(4-methoxyphenyl)butan-2-one + NADH + H+
-
-
-
?
(E)-4-(benzo[1,3]dioxol-5-yl)but-3-en-2-one + NAD+
4-(1,3-benzodioxol-5-yl)butan-2-one + NADH + H+
-
-
-
?
(E)-4-(benzo[1,3]dioxol-5-yl)but-3-en-2-one + NAD+
4-(1,3-benzodioxol-5-yl)butan-2-one + NADH + H+
-
-
-
?
(E)-4-methyl-2-pentenoate + NADH
4-methylpentanoate + NAD+
-
-
-
?
(E)-4-methyl-2-pentenoate + NADH
4-methylpentanoate + NAD+
-
-
-
?
(E)-4-methyl-2-pentenoate + NADH
4-methylpentanoate + NAD+
-
-
-
?
(E)-4-methyl-2-pentenoate + NADH
4-methylpentanoate + NAD+
-
-
-
?
(E)-4-methyl-2-pentenoate + NADH
4-methylpentanoate + NAD+
-
-
-
?
(E)-4-methyl-2-pentenoate + NADH
4-methylpentanoate + NAD+
-
-
-
?
(E)-4-methyl-2-pentenoate + NADH
4-methylpentanoate + NAD+
-
-
-
?
(E)-4-phenylbut-3-ene-2-one + NAD+
4-phenylbutan-2-one + NADH + H+
-
-
-
?
(E)-4-phenylbut-3-ene-2-one + NAD+
4-phenylbutan-2-one + NADH + H+
-
-
-
?
(E)-o-hydroxycinnamate + NADH
3-(2-hydroxyphenyl)propionate + NAD+
-
-
-
?
(E)-p-(dimethylamino)cinnamate + NADH
3-(4-(dimethylamino)phenyl)propionate + NAD+
-
-
-
?
(E)-p-(dimethylamino)cinnamate + NADH
3-(4-(dimethylamino)phenyl)propionate + NAD+
-
-
-
?
(E)-p-chlorocinnamate + NADH
3-(4-chlorophenyl)propionate + NAD+
-
-
-
?
(E)-p-methoxycinnamate + NADH
3-(4-methoxyphenyl)propionate + NAD+
-
-
-
?
(E)-p-nitrocinnamate + NADH
3-(4-nitrophenyl)propionate + NAD+
-
-
-
?
(R)-carvone + NADPH
2-methyl-5-(1-methylethenyl)cyclohexanone + NADP+
-
-
?
(R)-carvone + NADPH
2-methyl-5-(1-methylethenyl)cyclohexanone + NADP+
-
-
?
(R)-carvone + NADPH
2-methyl-5-(1-methylethenyl)cyclohexanone + NADP+
-
-
-
?
(Z)-2-(formylamino)cinnamate + NADH
3-(4-(formylamino)phenyl)propionate + NAD+
-
-
-
?
(Z)-2-(formylamino)cinnamate + NADH
3-(4-(formylamino)phenyl)propionate + NAD+
-
-
-
?
2-methyl-N-phenylmaleimide + NADH + H+
(3R)-3-methyl-1-phenylpyrrolidine-2,5-dione + NAD+
-
-
?
2-methyl-N-phenylmaleimide + NADH + H+
(3R)-3-methyl-1-phenylpyrrolidine-2,5-dione + NAD+
-
-
?
2-methyl-N-phenylmaleimide + NADPH
(3R)-3-methyl-1-phenylpyrrolidine-2,5-dione + NADP+
-
-
?
2-methyl-N-phenylmaleimide + NADPH
(3R)-3-methyl-1-phenylpyrrolidine-2,5-dione + NADP+
-
-
?
2-methylenesuccinic acid + NAD(P)H
(R)-2-methylsuccinic acid + NAD(P)+
-
-
-
?
2-methylenesuccinic acid + NAD(P)H
(R)-2-methylsuccinic acid + NAD(P)+
-
-
-
?
2-methylfumaric acid + NAD(P)H
2-methylsuccinic acid + NAD(P)+
-
-
-
?
2-methylmaleic acid + NAD(P)H
(R)-2-methylsuccinic acid + NAD(P)+
-
-
-
?
2-methylmaleic acid + NAD(P)H
(R)-2-methylsuccinic acid + NAD(P)+
-
-
-
?
5-benzylidenethiazolidine-2,4-dione + NADH
(5R)-5-benzyl-1,3-thiazolidine-2,4-dione + NAD+
-
-
-
?
5-benzylidenethiazolidine-2,4-dione + NADH
(5R)-5-benzyl-1,3-thiazolidine-2,4-dione + NAD+
-
-
-
?
butenoate + NADH
butanoate + NAD+
-
-
-
?
butenoate + NADH
butanoate + NAD+
-
-
-
?
butenoate + NADH
butanoate + NAD+
-
reverse reaction is energetically extremely unfavorable
-
?
butenoate + NADH
butanoate + NAD+
-
-
-
?
butenoate + NADH
butanoate + NAD+
-
-
-
?
dimethyl (2E)-2-methylbut-2-enedioate + NAD(P)H
dimethyl (2R)-2-methylbutanedioate + dimethyl (2S)-2-methylbutanedioate + NAD(P)+
-
-
-
?
dimethyl (2E)-2-methylbut-2-enedioate + NAD(P)H
dimethyl (2R)-2-methylbutanedioate + dimethyl (2S)-2-methylbutanedioate + NAD(P)+
-
-
-
?
dimethyl (2Z)-2-methylbut-2-enedioate + NAD(P)H
dimethyl (2R)-2-methylbutanedioate + NAD(P)+
-
-
-
?
dimethyl (2Z)-2-methylbut-2-enedioate + NAD(P)H
dimethyl (2R)-2-methylbutanedioate + NAD(P)+
-
-
-
?
dimethyl 2-methylidenebutanedioate + NAD(P)H
dimethyl (2R)-2-methylbutanedioate + NAD(P)+
-
-
-
?
dimethyl 2-methylidenebutanedioate + NAD(P)H
dimethyl (2R)-2-methylbutanedioate + NAD(P)+
-
-
-
?
N-ethylmaleimide + NADPH
N-ethylpyrrolidine-2,5-dione + NADP+
-
-
?
N-ethylmaleimide + NADPH
N-ethylpyrrolidine-2,5-dione + NADP+
-
-
?
additional information
?
-
-
enzyme may be part of the O2-dependent cytochrome P450-enzyme (+)-costunolide synthase, pathway, overview
-
?
additional information
?
-
-
transhydrogenase activity: reduction of 3-acetylpyridine adenine dinucleotide by NADH
-
?
additional information
?
-
-
enzyme is involved in the reduction of allyl alcohols
-
?
additional information
?
-
-
activity reaches a maximum in cells of the stationary phase
-
?
additional information
?
-
-
transhydrogenase activity: reduction of 3-acetylpyridine adenine dinucleotide by NADH
-
?
additional information
?
-
-
enzyme is involved in the reduction of allyl alcohols
-
?
additional information
?
-
-
reduction of enoate is coupled to the formation of ATP
-
?
additional information
?
-
the recombinant enzyme exhibits low activity for the conversion of the naringenin into phloretin
-
?
additional information
?
-
-
the recombinant enzyme exhibits low activity for the conversion of the naringenin into phloretin
-
?
additional information
?
-
NADPH does not react at all in the presence of 2-cyclohexen-1-one and rarely in presence of maleimide
-
?
additional information
?
-
NADPH does not react at all in the presence of 2-cyclohexen-1-one and rarely in presence of maleimide
-
?
additional information
?
-
-
NADPH does not react at all in the presence of 2-cyclohexen-1-one and rarely in presence of maleimide
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(+)-costunolide + NADPH
11(S),13-dihydrocostunolide + NADP+
-
part of the metabolization of (+)-costunolide to leucodin, overview
-
-
?
additional information
?
-
-
enzyme may be part of the O2-dependent cytochrome P450-enzyme (+)-costunolide synthase, pathway, overview
-
-
?
additional information
?
-
-
enzyme is involved in the reduction of allyl alcohols
-
-
?
additional information
?
-
-
activity reaches a maximum in cells of the stationary phase
-
-
?
additional information
?
-
-
enzyme is involved in the reduction of allyl alcohols
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Fe
Fe
-
iron-sulfur protein, contains 3.5-3.8 atoms of iron and 4.0 atoms of labile sulfur per subunit
Fe
-
presence of a [4Fe-4S]+2/+1 center, involvement of the iron-sulfur center in the enzyme mechanism
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
2-cyclohexen-1-one
-
the addition of 260 mM 2-cyclohexen-1-one leads to a more than 30fold decrease of the half-life at 30°C
2-methylbutenoate
-
in all reversed micellar solutions inhibition by the enoate is observed at an overall concentration of 0.5-5 mM, implying that a concentration of substrate equal to the Km value in aqueous solution may already cause inhibition in reversed micelles
O2
-
in the reduced state the enzyme is inactivated in 1-2 min, about 3 h after the removal of oxygen, the activity is partially restored
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
additional information
-
Km-value for NADH is lower than 0.02 mM
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
15.81
-
highest specific activity with 10 mM maleimide in 200 mM sodium phosphate, at pH 7.5, 0.2 mM NADPH, at 25°C
2.46
highest specific activity with 10 mM N-ethylmaleimide in 200 mM sodium phosphate, at pH 7.5, 0.2 mM NADPH, at 25°C
25.82
highest specific activity with 10 mM maleimide in 200 mM sodium phosphate, at pH 7.5, 0.2 mM NADPH, at 25°C
additional information
additional information
-
design and application of sensitive enzyme immunoassays specific for clostridial enoate reductase
additional information
-
design and application of sensitive enzyme immunoassays specific for clostridial enoate reductase
additional information
-
design and application of sensitive enzyme immunoassays specific for clostridial enoate reductase
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
30
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
brenda
xenobiotic reductase A showing enoate reductase activity
UniProt
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
Show AA Sequence and Transmembrane Helices (141 entries)
Please use the AA Sequence and Transmembrane Helices Search for a specific query.
Please use the AA Sequence and Transmembrane Helices Search for a specific query.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
70000
73000
73000
-
12 * 73000, equilibrium sedimentation in guanidine hydrochloride
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
dodecamer
dodecamer
-
12 * 73000, equilibrium sedimentation in guanidine hydrochloride
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
30 - 45
30 - 45
KYE1 has a half-life of 1146 min at 30°C, 210 min at 37°C, and less than 5 min at 45°C
30 - 45
XenA has a half-life of 600 min at 30°C, 99 min at 37°C, and less than 5 min at 45°C
30 - 45
-
Yers-ER has a half-life of more than 2160 min at 30°C, 1488 min at 37°C, and 117 min at 45°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
enzyme immobilized in calcium alginate gel, used in operation for 2 weeks, half-life of about 200 h
-
half-life of 150 h during operation, in the immobilized form on filter paper. Half-life: 350 h, electrode immobilized enzyme
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
OXIDATION STABILITY
ORGANISM
UNIPROT
LITERATURE
relatively stable in presence of oxygen, 50% loss of activity after 30 h
-
390672
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-18°C, pH 7, buffer containing 10 mM phosphate and 0.1 M KCl, formation of a dissociation product sedimenting at about 10 S
-
-20°C, phosphate buffer, pH 7.0, containing (E)-2-methyl-2-butenoate, EDTA, sucrose and 2-mercaptoethanol, repeatedly thawed and frozen, 50% loss of activity
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Ni2+-NTA bead chromatography
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
expressed in Escherichia coli BL21(DE3)
expressed in Escherichia coli growing under anaerobic conditions
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
RENATURED/Commentary
ORGANISM
UNIPROT
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Preiss, U.; White, H.; Simon, H.
Additional enoates amd other alpha,beta-unsaturated carbonyl compounds as substrates for the enoate reductase from Clostridium tyrobutyricum, influence of elevated hydrogen pressure on the reduction rate
DECHEMA Biotechnol. Conf.
3
189-192
1989
Clostridium tyrobutyricum
-
brenda
Krause, G.; Simon, H.
Design and application of sensitive enzyme immunoassays specific for clostridial enoate reductase
Z. Naturforsch. C
44
345-352
1989
Clostridium sporogenes, Moorella thermoacetica, Clostridium tyrobutyricum
brenda
Verhaert, R.M.D.; Tyrakowska, B.; Hilhorst, R.; Schaafsma, T.J.; Veeger, C.
Enzyme kinetics in reversed micelles. 2. Behaviour of enoate reductase
Eur. J. Biochem.
187
73-79
1990
Clostridium sp.
brenda
Thanos, I.; Deffner, A.; Simon, H.
Reductions of 2-enals, dehydrogenation of saturated aldehydes and their racemisation
Biol. Chem. Hoppe-Seyler
369
451-460
1988
Clostridium kluyveri, Clostridium tyrobutyricum
brenda
Thanos, I.; Bader, J.; Guenther, H.; Neumann, S.; Krauss, F.; Simon, H.
Electroenzymatic and electromicrobial reduction: preparation of chiral compounds
Methods Enzymol.
136
302-317
1987
Clostridium tyrobutyricum
-
brenda
Thanos, I.C.G.; Simon, H.
Electro-enzymic viologen-mediated stereospecific reduction of 2-enoates with free and immobilized enoate reductase on cellulose filters or modified carbon electrodes
J. Biotechnol.
6
13-29
1987
Clostridium tyrobutyricum
-
brenda
Kuno, S.; Bacher, A.; Simon, H.
Structure of enoate reductase from a Clostridium tyrobutyricum (C. spec. La1)
Biol. Chem. Hoppe-Seyler
366
463-472
1985
Clostridium tyrobutyricum
brenda
Bader, J.; Simon, H.
ATP formation is coupled to the hydrogenation of 2-enoates in Clostridium sporogenes
FEMS Microbiol. Lett.
20
171-175
1983
Clostridium sporogenes
-
brenda
Giesel, H.; Simon, H.
On the occurrence of enoate reductase and 2-oxo-carboxylate reductase in clostridia and some observations on the amino acid fermentation by Peptostreptococcus anaerobius
Arch. Microbiol.
135
51-57
1983
Paraclostridium bifermentans, Clostridium botulinum, Clostridioides difficile, Paeniclostridium ghonii, Tissierella praeacuta, Clostridioides mangenotii, Clostridium oceanicum, Paeniclostridium sordellii, Clostridium sporogenes, Acetoanaerobium sticklandii, no activity in Clostridium butyricum, no activity in Clostridium pasteurianum, no activity in Clostridium propionicum, Peptostreptococcus anaerobius
brenda
Giesel, H.; Simon, H.
Immunological relationship of enoate reductases from different clostridia and the classification of Clostridium species La 1
FEMS Microbiol. Lett.
19
43-45
1983
Clostridium kluyveri, Clostridium sp., Clostridium sporogenes, Clostridium tyrobutyricum
-
brenda
Buehler, M.; Simon, H.
On the kinetics and mechanism of enoate reductase
Hoppe-Seyler's Z. Physiol. Chem.
363
609-625
1982
Clostridium kluyveri, Clostridium sp.
brenda
Egerer, P.; Buehler, M.; Simon, H.
Rhein as an electron acceptor for various flavoproteins and for electron transport particles
Hoppe-Seyler's Z. Physiol. Chem.
363
627-633
1982
Clostridium sp.
brenda
Bader, J.; Kim, M.A.; Simon, H.
The reduction of allyl alcohols by Clostridium species is catalyzed by the combined action of alcohol dehydrogenase and enoate reductase
Hoppe-Seyler's Z. Physiol. Chem.
362
809-820
1981
Clostridium kluyveri, Clostridium sp.
brenda
Bader, J.; Simon, H.
The activities of hydrogenase and enoate reductase in two Clostridium species, their interrelationship and dependence on growth conditions
Arch. Microbiol.
127
279-287
1980
Clostridium kluyveri, Clostridium sp.
brenda
Buehler, M.; Giesel, H.; Tischer, W.; Simon, H.
Occurrence and the possible physiological role of 2-enoate reductases
FEBS Lett.
109
244-246
1980
Clostridium kluyveri, Clostridium sp., Clostridium sporogenes, Peptostreptococcus anaerobius
brenda
Tischer, W.; Bader, J.; Simon, H.
Purification and some properties of a hitherto-unknown enzyme reducing the carbon-carbon double bond of alpha, beta-unsaturated carboxylate anions
Eur. J. Biochem.
97
103-112
1979
Clostridium kluyveri, Clostridium sp.
brenda
Caldeira, J.; Feicht, R.; White, H.; Teixeira, M.; Moura, J.J.G.; Simon, H.; Moura, I.
EPR and Moessbauer spectroscopic studies on enoate reductase
J. Biol. Chem.
271
18743-18748
1996
Clostridium tyrobutyricum
brenda
Rohdich, F.; Wiese, A.; Feicht, R.; Simon, H.; Bacher, A.
Enoate reductases of Clostridia: cloning, sequencing, and expression
J. Biol. Chem.
276
5779-5787
2001
Clostridium tyrobutyricum (O52933), Moorella thermoacetica (O52935)
brenda
de Kraker, J.W.; Franssen, M.C.R.; Joerink, M.; de Groot, A.; Bouwmeester, H.J.
Biosynthesis of costunolide, dihydrocostunolide, and leucodin. Demostration of cytochrome P450-catalyzed formation of the lactone ring present in sesquiterpene lactones of chicory
Plant Physiol.
129
257-268
2002
Cichorium intybus
brenda
Chaparro-Riggers, J.F.; Rogers, T.A.; Vazquez-Figueroa, E.; Polizzi, K.M.; Bommarius, A.S.
Comparison of three enoate reductases and their potential use for biotransformations
Adv. Synth. Catal.
349
1521-1531
2007
Yersinia bercovieri, Kluyveromyces lactis (P40952), Pseudomonas putida (Q9R9V9)
-
brenda
Stuermer, R.; Hauer, B.; Hall, M.; Faber, K.
Asymmetric bioreduction of activated C:C bonds using enoate reductases from the old yellow enzyme family
Curr. Opin. Chem. Biol.
11
203-213
2007
Saccharomyces cerevisiae, Burkholderia sp., Marchantia polymorpha, Rhodotorula sp., Rhodotorula
brenda
Stueckler, C.; Hall, M.; Ehammer, H.; Pointner, E.; Kroutil, W.; Macheroux, P.; Faber, K.
Stereocomplementary bioreduction of alpha,beta-unsaturated dicarboxylic acids and dimethyl esters using enoate reductases: enzyme- and substrate-based stereocontrol
Org. Lett.
9
5409-5411
2007
Bacillus subtilis, Solanum lycopersicum
brenda
Richter, N.; Groeger, H.; Hummel, W.
Asymmetric reduction of activated alkenes using an enoate reductase from Gluconobacter oxydans
Appl. Microbiol. Biotechnol.
89
79-89
2011
Gluconobacter oxydans
brenda
Liu, Y.J.; Pei, X.Q.; Lin, H.; Gai, P.; Liu, Y.C.; Wu, Z.L.
Asymmetric bioreduction of activated alkenes by a novel isolate of Achromobacter species producing enoate reductase
Appl. Microbiol. Biotechnol.
95
635-645
2012
Achromobacter sp. (I3V5V5)
brenda
Wang, H.; Pei, X.; Wu, Z.
An enoate reductase Achr-OYE4 from Achromobacter sp. JA81: characterization and application in asymmetric bioreduction of CC bonds
Appl. Microbiol. Biotechnol.
98
705-15
2013
Achromobacter sp. (I3V5V6), Achromobacter sp. JA81 (I3V5V6)
brenda
Romagnolo, A.; Spina, F.; Carusetta, D.; Nerva, L.; Cramarossa, M.; Parmeggiani, F.; Forti, L.; Brenna, E.; Varese, G.
Fungal laccases and enoate reductases as biocatalysts of fine chemical transformations
Chem. Eng. Technol.
32
961-966
2013
Absidia glauca, Penicillium citrinum
-
brenda
Gao, X.; Ren, J.; Wu, Q.; Zhu, D.
Biochemical characterization and substrate profiling of a new NADH-dependent enoate reductase from Lactobacillus casei
Enzyme Microb. Technol.
51
26-34
2012
Lactobacillus casei
brenda
Iqbal, N.; Rudroff, F.; Brige, A.; Van Beeumen, J.; Mihovilovic, M.D.
Asymmetric bioreduction of activated carbon-carbon double bonds using Shewanella yellow enzyme (SYE-4) as novel enoate reductase
Tetrahedron
68
7619-7623
2012
Shewanella sp.
brenda
Gall, M.; Thomsen, M.; Peters, C.; Pavlidis, I.V.; Jonczyk, P.; Gruenert, P.P.; Beutel, S.; Scheper, T.; Gross, E.; Backes, M.; Geissler, T.; Ley, J.P.; Hilmer, J.M.; Krammer, G.; Palm, G.J.; Hinrichs, W.; Bornscheuer, U.T.
Enzymatic conversion of flavonoids using bacterial chalcone isomerase and enoate reductase
Angew. Chem. Int. Ed. Engl.
53
1439-1442
2014
Eubacterium ramulus (V9P074), Eubacterium ramulus
brenda
Skrobiszewski, A.; Ogorek, R.; Plaskowska, E.; Gladkowski, W.
Pleurotus ostreatus as a source of enoate reductase
Biocatal. Agricult. Biotechnol.
2
26-31
2013
Pleurotus ostreatus, Pleurotus ostreatus PO310783
-
brenda
Peters, C.; Rudroff, F.; Mihovilovic, M.D.; T Bornscheuer, U.
Fusion proteins of an enoate reductase and a Baeyer-Villiger monooxygenase facilitate the synthesis of chiral lactones
Biol. Chem.
398
31-37
2017
Pseudomonas putida
brenda
Classen, T.; Pietruszka, J.; Schuback, S.
Revisiting the enantioselective sequence patterns in enoate reductases
ChemCatChem
5
711-713
2013
Solanum lycopersicum, Photorhabdus luminescens
-
brenda
Sun, J.; Lin, Y.; Shen, X.; Jain, R.; Sun, X.; Yuan, Q.; Yan, Y.
Aerobic biosynthesis of hydrocinnamic acids in Escherichia coli with a strictly oxygen-sensitive enoate reductase
Metab. Eng.
35
75-82
2016
Clostridium acetobutylicum
brenda
Paul, C.; Gargiulo, S.; Opperman, D.; Lavandera, I.; Gotor-Fernandez, V.; Gotor, V.; Taglieber, A.; Arends, I.; Hollmann, F.
Mimicking nature: Synthetic nicotinamide cofactors for C=C bioreduction using enoate reductases
Org. Lett.
15
180-183
2013
Thermus scotoductus
brenda
Select items on the left to see more content.
EXTERNAL LINKS (specific for EC-Number 1.3.1.31)
html completed
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Information
