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Literature summary for 1.3.1.31 extracted from

  • Zhang, X.; Liao, S.; Cao, F.; Zhao, L.; Pei, J.; Tang, F.
    Cloning and characterization of enoate reductase with high beta-ionone to dihydro-beta-ionone bioconversion productivity (2018), BMC Biotechnol., 18, 26 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.3.1.31

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol 1 mM, 26.6% activation Artemisia annua

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 (DE3) Artemisia annua

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 1 mM, 14.2% loss of activity Artemisia annua
Cu2+ 1 mM, 83% loss of activity Artemisia annua
Fe2+ 5 mM, 21.4% loss of activity Artemisia annua
Fe3+ 5 mM, 99% loss of activity Artemisia annua
Hg2+ 1 mM, 97.9% loss of activity Artemisia annua
Li+ 1 mM, 10.1% loss of activity Artemisia annua
Mn2+ 1 mM, 21.7% loss of activity Artemisia annua
Zn2+ 5 mM, 62.9% loss of activity Artemisia annua

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.55
-
 beta-ionone pH 6.5, 45°C Artemisia annua

Metals/Ions

Metals/Ions Comment Organism Structure
Al3+ 5 mM, 25.9% increase in activity Artemisia annua
Ba2+ 5 mM, 26.5% increase in activity Artemisia annua
Co2+ 5 mM, 17.9% increase in activity Artemisia annua
Fe2+ 1 mM, 18% increase in activity Artemisia annua
K+ 1 mM, 11.7% increase in activity Artemisia annua
Li+ 5 mM, 18.3% increase in activity Artemisia annua
Mg2+ 5 mM, 23% increase in activity Artemisia annua
Mn2+ 5 mM, 14.5% increase in activity Artemisia annua
Na+ 1 mM, 22.5% increase in activity Artemisia annua
NH4+ 5 mM, 29.2% increase in activity Artemisia annua
Ni2+ 1 mM, 12.3% increase in activity Artemisia annua
Zn2+ 1 mM, 6.5% increase in activity Artemisia annua

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38500
-
 SDS-PAGE, protein fused with His-tag Artemisia annua

Organism

Organism UniProt Comment Textmining
Artemisia annua C0LNV1
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Artemisia annua

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2E-hexenal + NADPH + H+
-
 Artemisia annua ? + NADP+
-
 ?
2E-nonenal + NADPH + H+
-
 Artemisia annua ? + NADP+
-
 ?
artemisinic aldehyde + NADPH + H+
-
 Artemisia annua ? + NADP+
-
 ?
beta-ionone + NADPH + H+ DBR1 has high selectivity to hydrogenated the 10,11-unsaturated double bond of beta-ionone as well as high catalytic efficiency for the conversion of beta-ionone to dihydro-beta-ionone Artemisia annua dihydro-beta-ionone + NADP+
-
 ?

Subunits

Subunits Comment Organism
? x * 38500, SDS-PAGE, protein fused with His-tag Artemisia annua

Synonyms

Synonyms Comment Organism
Dbr1
-
 Artemisia annua

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Artemisia annua

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 50 30°C: about 70% of maximal activity, 50°C: about 80% of maximal activity Artemisia annua

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 100 min, pH 6.5, about 20% loss of activity Artemisia annua
45
-
 100 min, pH 6.5, about 25% loss of activity Artemisia annua
50
-
 100 min, pH 6.5, about 30% loss of activity Artemisia annua

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Artemisia annua

pH Range

pH Minimum pH Maximum Comment Organism
6 8 pH 6.0: about 85% of maximal activity, pH 8.0: about 80% of maximal activity Artemisia annua

pH Stability

pH Stability pH Stability Maximum Comment Organism
5
-
 40°C, 1 h, about 10% loss of activity Artemisia annua
6
-
 40°C, 1 h, no loss of activity Artemisia annua
7
-
 40°C, 1 h, about 5% loss of activity Artemisia annua
8
-
 40°C, 1 h, about 20% loss of activity Artemisia annua
9
-
 40°C, 1 h, about 30% loss of activity Artemisia annua