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Information on EC 1.2.7.B2 - formaldehyde ferredoxin oxidoreductase and Organism(s) Thermococcus litoralis and UniProt Accession Q56303

for references in articles please use BRENDA:EC1.2.7.B2
preliminary BRENDA-supplied EC number
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Thermococcus litoralis
UNIPROT: Q56303 not found.
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The taxonomic range for the selected organisms is: Thermococcus litoralis
The expected taxonomic range for this enzyme is: Bacteria, Archaea
Synonyms
formaldehyde ferredoxin oxidoreductase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SYSTEMATIC NAME
IUBMB Comments
formaldehyde:ferredoxin oxidoreductase
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acetaldehyde + H2O + oxidized methyl viologen
acetate + H+ + reduced methyl viologen
show the reaction diagram
-
-
-
?
butyraldehyde + H2O + oxidized methyl viologen
butyrate + H+ + reduced methyl viologen
show the reaction diagram
10% compared to the activity with acetyladehyde
-
-
?
formaldehyde + H2O + 2 oxidized ferredoxin
formate + 2 H+ + 2 reduced ferredoxin
show the reaction diagram
-
-
-
?
formaldehyde + H2O + oxidized methyl viologen
formate + H+ + reduced methyl viologen
show the reaction diagram
63% compared to the activity with acetyladehyde
-
-
?
propionaldehyde + H2O + oxidized methyl viologen
propionate + H+ + reduced methyl viologen
show the reaction diagram
77% compared to the activity with acetyladehyde
-
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Tungsten
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
arsenite
5 mM, 50% inhibition after min
cyanide
5 mM, 50% inhibition after min
iodoacetate
5 mM, 50% inhibition after min
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30000
gel filtration
68941
4 * 68941, calculated from sequence
70000
4 * 70000, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homotetramer
4 * 68941, calculated from sequence
tetramer
4 * 70000, SDS-PAGE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
80
2 h, 50% loss of activity
OXIDATION STABILITY
ORGANISM
UNIPROT
LITERATURE
the sensitivity to inactivation by O2, of formaldehyde ferredoxin oxidoreductase in the purified state is similar to that of the cell-free extract. That is, when the enzyme (5.0 mg/ml) as isolated under reducing conditions is briefly shaken in air to oxidize the sodium dithionite and then left exposed at 23°C, 50% of the original activity is lost after 5 h
644682
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Mukund, S.; Adams, M.W.
Characterization of a novel tungsten-containing formaldehyde ferredoxin oxidoreductase from the hyperthermophilic archaeon, Thermococcus litoralis. A role for tungsten in peptide catabolism
J. Biol. Chem.
268
13592-13600
1993
Thermococcus litoralis (Q56303), Thermococcus litoralis, Thermococcus litoralis DSM 5473 (Q56303)
Manually annotated by BRENDA team
Kletzin, A.; Mukund, S.; Kelley-Crouse, T.L.; Chan, M.K.; Rees, D.C.; Adams, M.W.
Molecular characterization of the genes encoding the tungsten-containing aldehyde ferredoxin oxidoreductase from Pyrococcus furiosus and formaldehyde ferredoxin oxidoreductase from Thermococcus litoralis
J. Bacteriol.
177
4817-4819
1995
Thermococcus litoralis (Q56303), Thermococcus litoralis
Manually annotated by BRENDA team
Dhawan, I.K.; Roy, R.; Koehler, B.P.; Mukund, S.; Adams, M.W.; Johnson, M.K.
Spectroscopic studies of the tungsten-containing formaldehyde ferredoxin oxidoreductase from the hyperthermophilic archaeon Thermococcus litoralis
J. Biol. Inorg. Chem.
5
313-327
2000
Thermococcus litoralis (Q56303), Thermococcus litoralis, Thermococcus litoralis DSM 5473 (Q56303)
Manually annotated by BRENDA team