BRENDA - Enzyme Database show
show all sequences of 1.2.7.B2

Characterization of a novel tungsten-containing formaldehyde ferredoxin oxidoreductase from the hyperthermophilic archaeon, Thermococcus litoralis. A role for tungsten in peptide catabolism

Mukund, S.; Adams, M.W.; J. Biol. Chem. 268, 13592-13600 (1993)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
arsenite
5 mM, 50% inhibition after min
Thermococcus litoralis
cyanide
5 mM, 50% inhibition after min
Thermococcus litoralis
iodoacetate
5 mM, 50% inhibition after min
Thermococcus litoralis
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Iron
the enzyme contains 16.0 gatom of iron per 280000 Da g of enzyme. Each subunit of dithionite reduced enzyme contains a magnetically isolated [4Fe-4S]1+ cluster that exists in both the S = 3/2 and S = 1/2 states
Thermococcus litoralis
Tungsten
tungsten-containing enzyme. The enzyme contains 3.3 gatom of tungsten per 280000 Da g of enzyme
Thermococcus litoralis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
30000
-
gel filtration
Thermococcus litoralis
70000
-
4 * 70000, SDS-PAGE
Thermococcus litoralis
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Thermococcus litoralis
Q56303
-
-
Thermococcus litoralis DSM 5473
Q56303
-
-
Oxidation Stability
Oxidation Stability
Organism
the sensitivity to inactivation by O2, of formaldehyde ferredoxin oxidoreductase in the purified state is similar to that of the cell-free extract. That is, when the enzyme (5.0 mg/ml) as isolated under reducing conditions is briefly shaken in air to oxidize the sodium dithionite and then left exposed at 23°C, 50% of the original activity is lost after 5 h
Thermococcus litoralis
Purification (Commentary)
Commentary
Organism
-
Thermococcus litoralis
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
6.8
-
pH 8.4, 80°C
Thermococcus litoralis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetaldehyde + H2O + oxidized methyl viologen
-
644682
Thermococcus litoralis
acetate + H+ + reduced methyl viologen
-
-
-
?
acetaldehyde + H2O + oxidized methyl viologen
-
644682
Thermococcus litoralis DSM 5473
acetate + H+ + reduced methyl viologen
-
-
-
?
butyraldehyde + H2O + oxidized methyl viologen
10% compared to the activity with acetyladehyde
644682
Thermococcus litoralis
butyrate + H+ + reduced methyl viologen
-
-
-
?
butyraldehyde + H2O + oxidized methyl viologen
10% compared to the activity with acetyladehyde
644682
Thermococcus litoralis DSM 5473
butyrate + H+ + reduced methyl viologen
-
-
-
?
formaldehyde + H2O + 2 oxidized ferredoxin
-
644682
Thermococcus litoralis
formate + 2 H+ + 2 reduced ferredoxin
-
-
-
?
formaldehyde + H2O + 2 oxidized ferredoxin
-
644682
Thermococcus litoralis DSM 5473
formate + 2 H+ + 2 reduced ferredoxin
-
-
-
?
formaldehyde + H2O + oxidized methyl viologen
63% compared to the activity with acetyladehyde
644682
Thermococcus litoralis
formate + H+ + reduced methyl viologen
-
-
-
?
formaldehyde + H2O + oxidized methyl viologen
63% compared to the activity with acetyladehyde
644682
Thermococcus litoralis DSM 5473
formate + H+ + reduced methyl viologen
-
-
-
?
additional information
activity with crotonaldehyde is less than 10% compared to activity with acetaldehyde: crotonaldehyde, benzaldehyde, phenylacetaldehyde
644682
Thermococcus litoralis
?
-
-
-
-
additional information
activity with crotonaldehyde is less than 10% compared to activity with acetaldehyde: crotonaldehyde, benzaldehyde, phenylacetaldehyde
644682
Thermococcus litoralis DSM 5473
?
-
-
-
-
propionaldehyde + H2O + oxidized methyl viologen
77% compared to the activity with acetyladehyde
644682
Thermococcus litoralis
propionate + H+ + reduced methyl viologen
-
-
-
?
Subunits
Subunits
Commentary
Organism
tetramer
4 * 70000, SDS-PAGE
Thermococcus litoralis
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
80
-
assay at
Thermococcus litoralis
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
80
-
2 h, 50% loss of activity
Thermococcus litoralis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.4
-
assay at
Thermococcus litoralis
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
arsenite
5 mM, 50% inhibition after min
Thermococcus litoralis
cyanide
5 mM, 50% inhibition after min
Thermococcus litoralis
iodoacetate
5 mM, 50% inhibition after min
Thermococcus litoralis
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Iron
the enzyme contains 16.0 gatom of iron per 280000 Da g of enzyme. Each subunit of dithionite reduced enzyme contains a magnetically isolated [4Fe-4S]1+ cluster that exists in both the S = 3/2 and S = 1/2 states
Thermococcus litoralis
Tungsten
tungsten-containing enzyme. The enzyme contains 3.3 gatom of tungsten per 280000 Da g of enzyme
Thermococcus litoralis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
30000
-
gel filtration
Thermococcus litoralis
70000
-
4 * 70000, SDS-PAGE
Thermococcus litoralis
Oxidation Stability (protein specific)
Oxidation Stability
Organism
the sensitivity to inactivation by O2, of formaldehyde ferredoxin oxidoreductase in the purified state is similar to that of the cell-free extract. That is, when the enzyme (5.0 mg/ml) as isolated under reducing conditions is briefly shaken in air to oxidize the sodium dithionite and then left exposed at 23°C, 50% of the original activity is lost after 5 h
Thermococcus litoralis
Purification (Commentary) (protein specific)
Commentary
Organism
-
Thermococcus litoralis
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
6.8
-
pH 8.4, 80°C
Thermococcus litoralis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetaldehyde + H2O + oxidized methyl viologen
-
644682
Thermococcus litoralis
acetate + H+ + reduced methyl viologen
-
-
-
?
acetaldehyde + H2O + oxidized methyl viologen
-
644682
Thermococcus litoralis DSM 5473
acetate + H+ + reduced methyl viologen
-
-
-
?
butyraldehyde + H2O + oxidized methyl viologen
10% compared to the activity with acetyladehyde
644682
Thermococcus litoralis
butyrate + H+ + reduced methyl viologen
-
-
-
?
butyraldehyde + H2O + oxidized methyl viologen
10% compared to the activity with acetyladehyde
644682
Thermococcus litoralis DSM 5473
butyrate + H+ + reduced methyl viologen
-
-
-
?
formaldehyde + H2O + 2 oxidized ferredoxin
-
644682
Thermococcus litoralis
formate + 2 H+ + 2 reduced ferredoxin
-
-
-
?
formaldehyde + H2O + 2 oxidized ferredoxin
-
644682
Thermococcus litoralis DSM 5473
formate + 2 H+ + 2 reduced ferredoxin
-
-
-
?
formaldehyde + H2O + oxidized methyl viologen
63% compared to the activity with acetyladehyde
644682
Thermococcus litoralis
formate + H+ + reduced methyl viologen
-
-
-
?
formaldehyde + H2O + oxidized methyl viologen
63% compared to the activity with acetyladehyde
644682
Thermococcus litoralis DSM 5473
formate + H+ + reduced methyl viologen
-
-
-
?
additional information
activity with crotonaldehyde is less than 10% compared to activity with acetaldehyde: crotonaldehyde, benzaldehyde, phenylacetaldehyde
644682
Thermococcus litoralis
?
-
-
-
-
additional information
activity with crotonaldehyde is less than 10% compared to activity with acetaldehyde: crotonaldehyde, benzaldehyde, phenylacetaldehyde
644682
Thermococcus litoralis DSM 5473
?
-
-
-
-
propionaldehyde + H2O + oxidized methyl viologen
77% compared to the activity with acetyladehyde
644682
Thermococcus litoralis
propionate + H+ + reduced methyl viologen
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
4 * 70000, SDS-PAGE
Thermococcus litoralis
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
80
-
assay at
Thermococcus litoralis
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
80
-
2 h, 50% loss of activity
Thermococcus litoralis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.4
-
assay at
Thermococcus litoralis
Other publictions for EC 1.2.7.B2
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
740555
Smalley
Functional and genomic diversi ...
Methyloversatilis discipulorum, Methyloversatilis discipulorum FAM1, Methyloversatilis discipulorum RZ18-153, Methyloversatilis discipulorum RZ94
Int. J. Syst. Evol. Microbiol.
65
2227-2233
2015
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722823
Liao
Why is the molybdenum-substitu ...
Pyrococcus furiosus
J. Biol. Inorg. Chem.
18
175-181
2013
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722893
Liao
Tungsten-dependent formaldehyd ...
Pyrococcus furiosus
J. Inorg. Biochem.
105
927-936
2011
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745210
Sevcenco
Molybdenum incorporation in t ...
Pyrococcus furiosus
J. Bacteriol.
192
4143-4152
2010
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1
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1
1
1
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723098
Sevcenco
The tungsten metallome of Pyro ...
Pyrococcus furiosus
Metallomics
1
395-402
2009
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1
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1
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674933
Bol
Redox chemistry of tungsten an ...
Pyrococcus furiosus
J. Biol. Inorg. Chem.
11
999-1006
2006
1
-
-
-
-
-
-
6
-
2
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5
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3
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1
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2
1
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1
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6
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3
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3
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1
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2
2
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2
2
674251
Bevers
WOR5, a novel tungsten-contain ...
Pyrococcus furiosus
J. Bacteriol.
187
7056-7061
2005
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3
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1
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644691
Roy
Aldehyde oxidoreductases from ...
Pyrococcus furiosus
Methods Enzymol.
331
132-144
2001
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7
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9
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745202
Adams
Key role for sulfur in peptid ...
Pyrococcus furiosus
J. Bacteriol.
183
716-724
2001
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722824
Dhawan
Spectroscopic studies of the t ...
Thermococcus litoralis, Thermococcus litoralis DSM 5473
J. Biol. Inorg. Chem.
5
313-327
2000
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644689
Roy
Purification and molecular cha ...
Pyrococcus furiosus
J. Bacteriol.
181
1171-1180
1999
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7
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644690
Hu
Formaldehyde ferredoxin oxidor ...
Pyrococcus furiosus
J. Mol. Biol.
286
899-914
1999
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722490
Mukund
Molybdenum and vanadium do not ...
Pyrococcus furiosus
J. Bacteriol.
178
163-167
1996
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644684
Kletzin
Molecular characterization of ...
Thermococcus litoralis
J. Bacteriol.
177
4817-4819
1995
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644682
Mukund
Characterization of a novel tu ...
Thermococcus litoralis, Thermococcus litoralis DSM 5473
J. Biol. Chem.
268
13592-13600
1993
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1
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11
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11
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