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Information on EC 1.2.1.22 - lactaldehyde dehydrogenase
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EC Tree
1.2.1.22 lactaldehyde dehydrogenaseSpecify your search results
Word Map
- 1.2.1.22
- aldolase
- l-lactate
- l-rhamnose
- glycol
- l-fucose
- dehydrogenases
- 1-phosphate
- methylglyoxal
- glycolaldehyde
-
nad+-dependent
- l-1,2-propanediol
-
propanediol
-
non-phosphorylated
-
permease
-
dissimilate
- dihydroxyacetone
The enzyme appears in viruses and cellular organisms
Synonyms
lactaldehyde dehydrogenase, l-lactaldehyde dehydrogenase, avladh, psladh, nad-dependent lactaldehyde dehydrogenase, nicotinamide adenine dinucleotide (nad)-linked dehydrogenase, mj1411, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
AldA
AN0544
AvLADH
dehydrogenase, lactaldehyde
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-
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L-lactaldehyde dehydrogenase
L-lactaldehyde:NAD oxidoreductase
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Lactaldehyde dehydrogenase
LADH
nicotinamide adenine dinucleotide (NAD)-linked dehydrogenase
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SSO3117
additional information
Lactaldehyde dehydrogenase
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-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
(S)-lactaldehyde + NAD+ + H2O = (S)-lactate + NADH + H+
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
oxidation
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redox reaction
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reduction
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PATHWAY SOURCE
PATHWAYS
BRENDA
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SYSTEMATIC NAME
IUBMB Comments
(S)-lactaldehyde:NAD+ oxidoreductase
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CAS REGISTRY NUMBER
COMMENTARY
37250-90-1
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(S)-lactaldehyde + NAD(P)+ + H2O
(S)-lactate + NAD(P)H + 2 H+
-
-
-
?
acrylaldehyde + NAD+ + H2O
acrylate + NADH
19% activity compared to D-lactaldehyde
-
-
?
crotonaldehyde + NAD+ + H2O
crotonate + NADH
84% activity compared to D-lactaldehyde
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-
?
DL-glyceraldehyde + NAD+ + H2O
glycerate + NADH
97% activity compared to D-lactaldehyde
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-
?
formaldehyde + NAD+ + H2O
formate + NADH
4% activity compared to D-lactaldehyde
-
-
?
propionaldehyde + NAD+ + H2O
propionate + NADH
187% activity compared to D-lactaldehyde
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-
?
propionaldehyde + NADP+ + H2O
propionate + NADPH + H+
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ALD wild-type only uses NAD+. F180T mutation renders an enzyme with the ability to use NADP+
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-
?
(R)-lactaldehyde + NAD+ + H2O
(R)-lactate + NADH + 2 H+
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8.6% of the activity of (S)-lactaldehyde
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-
?
(R)-lactaldehyde + NAD+ + H2O
(R)-lactate + NADH + 2 H+
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8.6% of the activity of (S)-lactaldehyde
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?
(R)-lactaldehyde + NAD+ + H2O
(R)-lactate + NADH + 2 H+
54% of the activity of L-lactaldehyde
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?
(R)-lactaldehyde + NAD+ + H2O
(R)-lactate + NADH + 2 H+
75% of the activity of (S)-lactaldehyde
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?
(S)-lactaldehyde + NAD+
(S)-lactate + NADH
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catabolism of L-threonine via amino acetone and methylglyoxal
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-
?
(S)-lactaldehyde + NAD+
(S)-lactate + NADH
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essential for catabolism of 1,2-propanediol
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?
(S)-lactaldehyde + NAD+
(S)-lactate + NADH
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aerobic metabolism of fucose
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-
?
(S)-lactaldehyde + NAD+
(S)-lactate + NADH
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general role in aldehyde oxidation, involved in several metabolic pathways
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?
(S)-lactaldehyde + NAD+ + H2O
(S)-lactate + NADH + 2 H+
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?
(S)-lactaldehyde + NAD+ + H2O
(S)-lactate + NADH + 2 H+
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-
-
?
(S)-lactaldehyde + NAD+ + H2O
(S)-lactate + NADH + 2 H+
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?
(S)-lactaldehyde + NAD+ + H2O
(S)-lactate + NADH + 2 H+
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?
(S)-lactaldehyde + NAD+ + H2O
(S)-lactate + NADH + 2 H+
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?
(S)-lactaldehyde + NAD+ + H2O
(S)-lactate + NADH + 2 H+
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?
(S)-lactaldehyde + NAD+ + H2O
(S)-lactate + NADH + H+
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best substrate
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?
(S)-lactaldehyde + NAD+ + H2O
(S)-lactate + NADH + H+
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best substrate
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?
(S)-lactaldehyde + NAD+ + H2O
(S)-lactate + NADH + H+
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?
(S)-lactaldehyde + NAD+ + H2O
(S)-lactate + NADH + H+
best substrate
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?
(S)-lactaldehyde + NADP+ + H2O
(S)-lactate + NADPH + 2 H+
assay using NADP+
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?
(S)-lactaldehyde + NADP+ + H2O
(S)-lactate + NADPH + 2 H+
assay using NADP+
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?
acetaldehyde + NAD+ + H2O
acetate + NADH + H+
25% activity compared to D-lactaldehyde
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?
D-lactaldehyde + NAD+ + H2O
D-lactate + NADH + H+
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56% the rate of L-lactaldehyde reduction
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?
D-lactaldehyde + NAD+ + H2O
D-lactate + NADH + H+
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?
D-lactaldehyde + NAD+ + H2O
D-lactate + NADH + H+
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0.2% the activity of L-lactaldehyde reduction
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?
glycolaldehyde + NAD+ + H2O
glycolate + NADH
35% activity compared to D-lactaldehyde
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?
glycolaldehyde + NAD+ + H2O
glycolate + NADH + 2 H+
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?
glycolaldehyde + NAD+ + H2O
glycolate + NADH + 2 H+
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?
additional information
?
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oxidizes several aldehydes, e.g. L-glyceraldehyde, glycolaldehyde, methylglyoxal
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?
additional information
?
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not: acetaldehyde, formaldehyde, propionaldehyde, succinic semialdehyde
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?
additional information
?
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specifically oxidizes L-lactaldehyde to L-lactate in presence of NAD+
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?
additional information
?
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residue Asn286 of L-lactaldehyde dehydrogenase plays an important structure role to substrate identification. The wild-type enzyme is not active with D-glyceraldehyde, methylglyoxal, acetaldehyde, L-lactate, or benzaldehyde
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?
additional information
?
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residue Asn286 of L-lactaldehyde dehydrogenase plays an important structure role to substrate identification. The wild-type enzyme is not active with D-glyceraldehyde, methylglyoxal, acetaldehyde, L-lactate, or benzaldehyde
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?
additional information
?
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the enzyme shows NAD+-dependent l-lactaldehyde dehydrogenase activity in the methylglyoxyl metabolism, metabolizing methylglyoxal via methylglyoxal reductase and lactaldehyde dehydrogenase to L-lactate, overview
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?
additional information
?
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the enzyme shows NAD+-dependent l-lactaldehyde dehydrogenase activity in the methylglyoxyl metabolism, metabolizing methylglyoxal via methylglyoxal reductase and lactaldehyde dehydrogenase to L-lactate, overview
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?
additional information
?
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not: acetaldehyde, DL-glyceraldehyde, propionaldehyde, phenylpyruvate
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?
additional information
?
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specifically oxidizes L-lactaldehyde to L-lactate in presence of NAD+
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?
additional information
?
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the enzyme shows NAD+-dependent l-lactaldehyde dehydrogenase activity in the methylglyoxyl metabolism, metabolizing methylglyoxal via methylglyoxal reductase and lactaldehyde dehydrogenase to L-lactate, overview
-
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?
additional information
?
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the enzyme shows NAD+-dependent l-lactaldehyde dehydrogenase activity in the methylglyoxyl metabolism, metabolizing methylglyoxal via methylglyoxal reductase and lactaldehyde dehydrogenase to L-lactate, overview
-
-
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(S)-lactaldehyde + NAD(P)+ + H2O
(S)-lactate + NAD(P)H + 2 H+
-
-
-
?
(S)-lactaldehyde + NAD+
(S)-lactate + NADH
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catabolism of L-threonine via amino acetone and methylglyoxal
-
-
?
(S)-lactaldehyde + NAD+
(S)-lactate + NADH
-
essential for catabolism of 1,2-propanediol
-
-
?
(S)-lactaldehyde + NAD+
(S)-lactate + NADH
-
aerobic metabolism of fucose
-
-
?
(S)-lactaldehyde + NAD+
(S)-lactate + NADH
-
general role in aldehyde oxidation, involved in several metabolic pathways
-
-
?
(S)-lactaldehyde + NAD+ + H2O
(S)-lactate + NADH + 2 H+
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?
(S)-lactaldehyde + NAD+ + H2O
(S)-lactate + NADH + 2 H+
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?
(S)-lactaldehyde + NAD+ + H2O
(S)-lactate + NADH + 2 H+
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?
(S)-lactaldehyde + NAD+ + H2O
(S)-lactate + NADH + 2 H+
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?
(S)-lactaldehyde + NAD+ + H2O
(S)-lactate + NADH + 2 H+
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?
additional information
?
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the enzyme shows NAD+-dependent l-lactaldehyde dehydrogenase activity in the methylglyoxyl metabolism, metabolizing methylglyoxal via methylglyoxal reductase and lactaldehyde dehydrogenase to L-lactate, overview
-
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?
additional information
?
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the enzyme shows NAD+-dependent l-lactaldehyde dehydrogenase activity in the methylglyoxyl metabolism, metabolizing methylglyoxal via methylglyoxal reductase and lactaldehyde dehydrogenase to L-lactate, overview
-
-
?
additional information
?
-
-
the enzyme shows NAD+-dependent l-lactaldehyde dehydrogenase activity in the methylglyoxyl metabolism, metabolizing methylglyoxal via methylglyoxal reductase and lactaldehyde dehydrogenase to L-lactate, overview
-
-
?
additional information
?
-
-
the enzyme shows NAD+-dependent l-lactaldehyde dehydrogenase activity in the methylglyoxyl metabolism, metabolizing methylglyoxal via methylglyoxal reductase and lactaldehyde dehydrogenase to L-lactate, overview
-
-
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
NAD+
-
ALD only uses NAD+. Residue F180 of ALD may participate in coenzyme specificity
NAD+
cofactor flexibility in the constricted active site plays a significant role in the mechanism of oxidation of lactaldehyde to lactate
additional information
the negatively charged carboxylate group of E179 destabilizes the binding of the 2'-phosphate group of NADPH sterically and electrostatically, thereby accounting for the lack of enzyme activity with this cofactor
-
additional information
-
the negatively charged carboxylate group of E179 destabilizes the binding of the 2'-phosphate group of NADPH sterically and electrostatically, thereby accounting for the lack of enzyme activity with this cofactor
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
-
iodoacetamide, Mn2+, Mg2+, Co2+, no effect at 5 mM
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
15.2
acetaldehyde
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100 mM H2NaPO4 pH 7.5, 100 mM NaCl, 20 mM 2-mercaptoethanol, 2 mM NAD+
0.15
benzaldehyde
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100 mM H2NaPO4 pH 7.5, 100 mM NaCl, 20 mM 2-mercaptoethanol, 2 mM NAD+
0.14
glycoaldehyde
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100 mM H2NaPO4 pH 7.5, 100 mM NaCl, 20 mM 2-mercaptoethanol, 2 mM NAD+