BRENDA - Enzyme Database
show all sequences of 1.2.1.22

Characterization of E. coli tetrameric aldehyde dehydrogenases with atypical properties compared to other aldehyde dehydrogenases

Rodriguez-Zavala, J.S.; Allali-Hassani, A.; Weiner, H.; Protein Sci. 15, 1387-1396 (2006)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli BL21 as His-tag fusion protein
Escherichia coli
Inhibitors
Inhibitors
Commentary
Organism
Structure
Mg2+
56% inhibition at 10 mM
Escherichia coli
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.14
-
glycoaldehyde
100 mM H2NaPO4 pH 7.5, 100 mM NaCl, 20 mM 2-mercaptoethanol, 2 mM NAD+
Escherichia coli
0.15
-
benzaldehyde
100 mM H2NaPO4 pH 7.5, 100 mM NaCl, 20 mM 2-mercaptoethanol, 2 mM NAD+
Escherichia coli
0.24
-
propionaldehyde
100 mM H2NaPO4 pH 7.5, 100 mM NaCl, 20 mM 2-mercaptoethanol, 2 mM NAD+
Escherichia coli
4.5
-
phenaylacetaldehyd
100 mM H2NaPO4 pH 7.5, 100 mM NaCl, 20 mM 2-mercaptoethanol, 2 mM NAD+
Escherichia coli
15.2
-
acetaldehyde
100 mM H2NaPO4 pH 7.5, 100 mM NaCl, 20 mM 2-mercaptoethanol, 2 mM NAD+
Escherichia coli
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
210000
-
gel-filtration
Escherichia coli
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
-
-
Purification (Commentary)
Commentary
Organism
Ni-affinity chromatography
Escherichia coli
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Escherichia coli
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetaldehyde + NAD+ + H2O
-
670833
Escherichia coli
acetate + NADH + H+
-
-
-
?
benzaldehyde + NAD+ + H2O
-
670833
Escherichia coli
benzoate + NADH + H+
-
-
-
?
glycoaldehyde + NAD+ + H2O
-
670833
Escherichia coli
glycolate + NADH + H+
-
-
-
?
phenylacetaldehyde + NAD+ + H2O
-
670833
Escherichia coli
phenylacetate + NADH + H+
-
-
-
?
propionaldehyde + NAD+ + H2O
-
670833
Escherichia coli
propionate + NADH + H+
-
-
-
?
Subunits
Subunits
Commentary
Organism
tetramer
calculated from sequence, gel-filtration
Escherichia coli
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.5
-
phenaylacetaldehyd
100 mM H2NaPO4 pH 7.5, 100 mM NaCl, 20 mM 2-mercaptoethanol, 2 mM NAD+
Escherichia coli
0.6
-
acetaldehyde
100 mM H2NaPO4 pH 7.5, 100 mM NaCl, 20 mM 2-mercaptoethanol, 2 mM NAD+
Escherichia coli
0.9
-
benzaldehyde
100 mM H2NaPO4 pH 7.5, 100 mM NaCl, 20 mM 2-mercaptoethanol, 2 mM NAD+
Escherichia coli
1.8
-
propionaldehyde
100 mM H2NaPO4 pH 7.5, 100 mM NaCl, 20 mM 2-mercaptoethanol, 2 mM NAD+
Escherichia coli
18.3
-
glycoaldehyde
100 mM H2NaPO4 pH 7.5, 100 mM NaCl, 20 mM 2-mercaptoethanol, 2 mM NAD+
Escherichia coli
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Escherichia coli
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli BL21 as His-tag fusion protein
Escherichia coli
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Escherichia coli
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Mg2+
56% inhibition at 10 mM
Escherichia coli
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.14
-
glycoaldehyde
100 mM H2NaPO4 pH 7.5, 100 mM NaCl, 20 mM 2-mercaptoethanol, 2 mM NAD+
Escherichia coli
0.15
-
benzaldehyde
100 mM H2NaPO4 pH 7.5, 100 mM NaCl, 20 mM 2-mercaptoethanol, 2 mM NAD+
Escherichia coli
0.24
-
propionaldehyde
100 mM H2NaPO4 pH 7.5, 100 mM NaCl, 20 mM 2-mercaptoethanol, 2 mM NAD+
Escherichia coli
4.5
-
phenaylacetaldehyd
100 mM H2NaPO4 pH 7.5, 100 mM NaCl, 20 mM 2-mercaptoethanol, 2 mM NAD+
Escherichia coli
15.2
-
acetaldehyde
100 mM H2NaPO4 pH 7.5, 100 mM NaCl, 20 mM 2-mercaptoethanol, 2 mM NAD+
Escherichia coli
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
210000
-
gel-filtration
Escherichia coli
Purification (Commentary) (protein specific)
Commentary
Organism
Ni-affinity chromatography
Escherichia coli
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Escherichia coli
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetaldehyde + NAD+ + H2O
-
670833
Escherichia coli
acetate + NADH + H+
-
-
-
?
benzaldehyde + NAD+ + H2O
-
670833
Escherichia coli
benzoate + NADH + H+
-
-
-
?
glycoaldehyde + NAD+ + H2O
-
670833
Escherichia coli
glycolate + NADH + H+
-
-
-
?
phenylacetaldehyde + NAD+ + H2O
-
670833
Escherichia coli
phenylacetate + NADH + H+
-
-
-
?
propionaldehyde + NAD+ + H2O
-
670833
Escherichia coli
propionate + NADH + H+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
calculated from sequence, gel-filtration
Escherichia coli
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.5
-
phenaylacetaldehyd
100 mM H2NaPO4 pH 7.5, 100 mM NaCl, 20 mM 2-mercaptoethanol, 2 mM NAD+
Escherichia coli
0.6
-
acetaldehyde
100 mM H2NaPO4 pH 7.5, 100 mM NaCl, 20 mM 2-mercaptoethanol, 2 mM NAD+
Escherichia coli
0.9
-
benzaldehyde
100 mM H2NaPO4 pH 7.5, 100 mM NaCl, 20 mM 2-mercaptoethanol, 2 mM NAD+
Escherichia coli
1.8
-
propionaldehyde
100 mM H2NaPO4 pH 7.5, 100 mM NaCl, 20 mM 2-mercaptoethanol, 2 mM NAD+
Escherichia coli
18.3
-
glycoaldehyde
100 mM H2NaPO4 pH 7.5, 100 mM NaCl, 20 mM 2-mercaptoethanol, 2 mM NAD+
Escherichia coli
Other publictions for EC 1.2.1.22
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
740187
Wu
A new NAD(+)-dependent glycera ...
Escherichia coli
Biosci. Biotechnol. Biochem.
80
2306-2310
2016
-
-
-
-
3
-
-
4
-
-
-
-
-
2
-
-
-
-
-
-
4
-
1
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-
-
1
4
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1
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1
-
3
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-
-
4
-
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-
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-
4
-
1
-
-
-
1
4
-
-
-
-
-
-
-
-
4
4
742119
Wu
A new NAD+-dependent glyceral ...
Escherichia coli
Biosci. Biotechnol. Biochem.
80
2306-2310
2016
-
-
1
-
22
-
-
1
-
-
-
1
-
2
-
-
1
-
-
-
4
-
2
1
3
-
1
-
1
-
-
1
-
-
-
-
-
1
1
-
22
-
-
-
-
1
-
-
-
1
-
-
-
1
-
-
4
-
2
1
3
-
1
-
1
-
-
-
-
1
1
-
-
-
743302
Wolf
A systems biology approach re ...
Saccharolobus solfataricus, Saccharolobus solfataricus P2
Mol. Microbiol.
102
882-908
2016
-
-
-
-
-
-
-
-
-
-
-
2
-
4
-
-
-
-
-
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4
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-
-
-
-
-
-
-
1
-
-
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-
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-
1
-
-
-
-
-
-
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-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
692329
Greig
A comparative study of methylg ...
Leishmania major, Trypanosoma brucei, Trypanosoma cruzi
FEBS J.
276
376-386
2009
-
-
-
-
-
-
-
-
-
-
-
8
-
6
-
-
-
-
-
5
3
-
8
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
-
5
3
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
695015
Rodriguez-Zavala
Enhancement of coenzyme bindin ...
Escherichia coli
Protein Sci.
17
563-570
2008
-
-
1
-
1
-
1
5
-
-
-
-
-
2
-
-
1
-
-
-
-
1
5
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
1
-
-
1
-
5
-
-
-
-
-
-
-
1
-
-
-
1
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
697876
Watanabe
Metabolic fate of L-lactaldehy ...
Azotobacter vinelandii, Azotobacter vinelandii NBRC 102612, Scheffersomyces stipitis
FEBS J.
275
5139-5149
2008
-
-
2
-
-
-
-
-
1
1
1
4
-
10
-
-
2
-
-
-
11
-
15
1
-
-
-
-
-
-
-
4
-
-
-
-
-
3
7
-
-
-
-
-
-
-
1
1
2
4
-
-
-
3
-
-
11
-
15
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
670833
Rodriguez-Zavala
Characterization of E. coli te ...
Escherichia coli
Protein Sci.
15
1387-1396
2006
-
-
1
-
-
-
1
5
-
-
1
-
-
1
-
-
1
-
-
1
-
-
5
1
-
-
-
5
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
1
-
5
-
-
1
-
-
-
-
1
-
1
-
-
5
1
-
-
-
5
-
-
-
-
-
-
-
-
-
-
674270
Grochowski
Identification of lactaldehyde ...
Methanocaldococcus jannaschii
J. Bacteriol.
188
2836-2844
2006
-
-
1
-
-
-
2
2
-
-
2
-
-
2
-
-
1
-
-
1
1
-
8
1
-
-
3
2
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
2
-
-
2
-
-
-
-
1
-
1
1
-
8
1
-
-
3
2
1
1
-
-
-
-
-
-
-
-
675430
Di Costanzo
Crystal structure of lactaldeh ...
Escherichia coli
J. Mol. Biol.
366
481-493
2006
-
-
1
1
-
-
-
-
-
-
1
-
-
3
-
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1
-
-
1
-
-
1
1
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-
-
-
-
-
2
-
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-
-
1
2
1
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288134
Baldoma
Metabolism of L-fucose and L-r ...
Escherichia coli
J. Bacteriol.
170
416-421
1988
-
-
-
-
-
-
-
-
-
-
-
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2
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1
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1
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1
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1
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-
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-
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288133
Baldoma
Involvement of lactaldehyde de ...
Escherichia coli
J. Biol. Chem.
262
13991-13996
1987
-
-
-
-
-
-
9
6
-
-
2
1
-
3
-
-
1
-
-
-
1
-
7
1
-
-
-
-
-
-
-
1
-
-
-
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-
-
1
-
-
-
-
9
-
6
-
-
2
1
-
-
-
1
-
-
1
-
7
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288132
Inoue
Metabolism of 2-oxoaldehydes i ...
Saccharomyces cerevisiae
Eur. J. Biochem.
153
243-247
1985
2
-
-
-
-
1
7
2
-
3
1
-
-
4
-
-
1
-
-
-
1
1
4
-
1
1
1
-
1
-
1
2
-
-
-
2
-
-
2
-
-
1
-
7
-
2
-
3
1
-
-
-
-
1
-
-
1
1
4
-
1
1
1
-
1
-
1
-
-
-
-
-
-
-
288131
Caballero
Identification of lactaldehyde ...
Escherichia coli
J. Biol. Chem.
258
7788-7792
1983
-
-
-
-
-
-
-
-
-
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-
1
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3
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1
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2
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3
-
1
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1
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1
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1
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1
-
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2
-
3
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
287313
Hacking
Disruption of the fucose pathw ...
Escherichia coli
J. Bacteriol.
126
1166-1172
1976
-
-
-
-
-
-
-
-
-
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-
-
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2
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1
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1
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1
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1
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287315
Cocks
Evolution of L-1,2-propanediol ...
Escherichia coli
J. Bacteriol.
118
83-88
1974
-
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-
-
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-
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1
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1
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1
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1
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1
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-
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-
-
-
288136
Willetts
Threonine metabolism in a stra ...
Bacillus subtilis
Biochim. Biophys. Acta
222
234-236
1970
-
-
-
-
-
-
-
3
-
-
1
1
-
1
-
-
1
-
-
-
-
-
3
-
-
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-
1
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1
-
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1
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-
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3
-
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1
1
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-
1
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-
3
-
-
-
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-
1
-
-
-
-
-
-
-
-
-
288135
Sridhara
Purification and properties of ...
Escherichia coli
J. Biol. Chem.
244
5233-5238
1969
2
-
-
-
-
1
2
1
-
-
1
1
-
2
-
-
1
-
-
-
1
-
3
-
-
-
-
-
1
1
-
2
-
-
-
2
-
-
2
-
-
1
-
2
-
1
-
-
1
1
-
-
-
1
-
-
1
-
3
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-