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EC Tree
IUBMB Comments A cytochrome P-450 (heme-thiolate) protein isolated from the plant Croton sublyratus.
The enzyme appears in viruses and cellular organisms
Synonyms
G10H, geraniol 10-hydroxylase, geranylgeraniol-18-hydroxylase, GGOH-18-hydroxylase,
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geranylgeraniol-18-hydroxylase
G10H
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G10H
Vitis vinifera x Vitis vinifera
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geraniol 10-hydroxylase
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geraniol 10-hydroxylase
Vitis vinifera x Vitis vinifera
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geranylgeraniol-18-hydroxylase
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geranylgeraniol-18-hydroxylase
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geranylgeraniol-18-hydroxylase
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GGOH-18-hydroxylase
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GGOH-18-hydroxylase
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geranylgeraniol + [reduced NADPH-hemoprotein reductase] + O2 = 18-hydroxygeranylgeraniol + [oxidized NADPH-hemoprotein reductase] + H2O
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geranylgeraniol,[reduced NADPH-hemoprotein reductase]:oxygen oxidoreductase (18-hydroxylating)
A cytochrome P-450 (heme-thiolate) protein isolated from the plant Croton sublyratus.
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geranylgeraniol + [reduced NADPH-hemoprotein reductase] + O2
18-hydroxygeranylgeraniol + [oxidized NADPH-hemoprotein reductase] + H2O
geranylgeraniol + [reduced NADPH-hemoprotein reductase] + O2
18-hydroxygeranylgeraniol + [oxidized NADPH-hemoprotein reductase] + H2O
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last step of plaunotol biosynthesis
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?
geranylgeraniol + [reduced NADPH-hemoprotein reductase] + O2
18-hydroxygeranylgeraniol + [oxidized NADPH-hemoprotein reductase] + H2O
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last step of plaunotol biosynthesis
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?
geranylgeraniol + [reduced NADPH-hemoprotein reductase] + O2
18-hydroxygeranylgeraniol + [oxidized NADPH-hemoprotein reductase] + H2O
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?
geranylgeraniol + [reduced NADPH-hemoprotein reductase] + O2
18-hydroxygeranylgeraniol + [oxidized NADPH-hemoprotein reductase] + H2O
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this enzyme is involved in the final step of the biosynthetic pathway of plaunotol, an antipeptic ulcer constituent accumulated in this plant
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?
geranylgeraniol + [reduced NADPH-hemoprotein reductase] + O2
18-hydroxygeranylgeraniol + [oxidized NADPH-hemoprotein reductase] + H2O
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NADH shows about 70% of the activity with NADPH
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?
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geranylgeraniol + [reduced NADPH-hemoprotein reductase] + O2
18-hydroxygeranylgeraniol + [oxidized NADPH-hemoprotein reductase] + H2O
geranylgeraniol + [reduced NADPH-hemoprotein reductase] + O2
18-hydroxygeranylgeraniol + [oxidized NADPH-hemoprotein reductase] + H2O
-
last step of plaunotol biosynthesis
-
-
?
geranylgeraniol + [reduced NADPH-hemoprotein reductase] + O2
18-hydroxygeranylgeraniol + [oxidized NADPH-hemoprotein reductase] + H2O
-
last step of plaunotol biosynthesis
-
-
?
geranylgeraniol + [reduced NADPH-hemoprotein reductase] + O2
18-hydroxygeranylgeraniol + [oxidized NADPH-hemoprotein reductase] + H2O
-
this enzyme is involved in the final step of the biosynthetic pathway of plaunotol, an antipeptic ulcer constituent accumulated in this plant
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-
?
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additional information
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improvement of thin-layer chromatography for enzyme assay of geranylgeraniol 18-hydroxylase
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additional information
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above and below the pH optimum (pH 5) activity declines rapidly and is undetectable after 60 min
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Ohba
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brenda
Ohba
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brenda
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brenda
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brenda
Vitis vinifera x Vitis vinifera
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brenda
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brenda
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brenda
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brenda
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physiological function
Vitis vinifera x Vitis vinifera
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involved in terpenoid metabolism
physiological function
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involved in terpenoid metabolism
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100
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activity in the heated cell-free extract increases steadily during the first 30 min of the treatment. Thereafter, the enzyme activity declines rapidly and is almost undetectable after 60 min
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Chanama, M.; Wunnakup, T.; De-Eknamkul, W.; Chanama, S.
Improvement of thin-layer chromatography for enzyme assay of geranylgeraniol 18-hydroxylase from Croton stellatopilosus Ohba
J. Planar Chromatogr.
22
49-53
2009
Croton stellatopilosus, Croton stellatopilosus Ohba
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brenda
Tansakul, P.; De-Eknamkul, W.
Geranylgeraniol-18-hydroxylase: the last enzyme in the plaunotol biosynthetic pathway in Croton sublyratus
Phytochemistry
47
1241-1246
1998
Croton sublyratus
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brenda
Battilana, J.; Costantini, L.; Emanuelli, F.; Sevini, F.; Segala, C.; Moser, S.; Velasco, R.; Versini, G.; Stella Grando, M.
The 1-deoxy-D: -xylulose 5-phosphate synthase gene co-localizes with a major QTL affecting monoterpene content in grapevine
Theor. Appl. Genet.
118
653-669
2009
Vitis vinifera x Vitis vinifera, Vitis vinifera x Vitis riparia
brenda
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1.14.14.146 geranylgeraniol 18-hydroxylase
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