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Information on EC 1.14.11.9 - flavanone 3-dioxygenase and Organism(s) Oryza sativa and UniProt Accession Q8W2X5

for references in articles please use BRENDA:EC1.14.11.9
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EC Tree
IUBMB Comments
Requires Fe2+ and ascorbate. This plant enzyme catalyses an early step in the flavonoid biosynthesis pathway, leading to the production of flavanols and anthocyanins. Substrates include (2S)-naringenin, (2S)-eriodictyol, (2S)-dihydrotricetin and (2S)-pinocembrin. Some enzymes are bifuctional and also catalyse EC 1.14.20.6, flavonol synthase.
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This record set is specific for:
Oryza sativa
UNIPROT: Q8W2X5
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Word Map
The taxonomic range for the selected organisms is: Oryza sativa
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
flavanone 3-hydroxylase, flavanone-3-hydroxylase, flavanone 3beta-hydroxylase, csf3h, lcf3h, aaf3h, ctf3h, naringenin 3-dioxygenase, acf3h, pef3h, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
(2S)-flavanone 3-hydroxylase
-
-
-
-
F3H
-
-
-
-
FHTPH
-
-
-
-
flavanone 3-hydroxylase
-
-
-
-
flavanone 3beta-hydroxylase
-
-
-
-
flavanone synthase I
-
-
-
-
FS I
-
-
-
-
naringenin 3-dioxygenase
-
-
-
-
naringenin,2-oxoglutarate:oxygen oxidoreductase (3-hydroxylating)
-
-
-
-
oxygenase, flavanone 3-di-
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
redox reaction
-
-
-
-
oxidation
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-
-
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reduction
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-
-
-
PATHWAY SOURCE
PATHWAYS
-
-, -, -, -, -
SYSTEMATIC NAME
IUBMB Comments
(2S)-flavan-4-one,2-oxoglutarate:oxygen oxidoreductase (3-hydroxylating)
Requires Fe2+ and ascorbate. This plant enzyme catalyses an early step in the flavonoid biosynthesis pathway, leading to the production of flavanols and anthocyanins. Substrates include (2S)-naringenin, (2S)-eriodictyol, (2S)-dihydrotricetin and (2S)-pinocembrin. Some enzymes are bifuctional and also catalyse EC 1.14.20.6, flavonol synthase.
CAS REGISTRY NUMBER
COMMENTARY hide
75991-43-4
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2S)-naringenin + 2-oxoglutarate + O2
(2R,3R)-dihydrokaempferol + succinate + CO2
show the reaction diagram
-
-
-
?
(S)-eriodictyol + 2-oxoglutarate + O2
(2R,3R)-dihydroquercetin + succinate + CO2
show the reaction diagram
-
-
-
?
3'-methoxy eriodictyol + 2-oxoglutarate + O2
? + succinate + CO2
show the reaction diagram
-
-
-
?
4'-methoxy eriodictyol + 2-oxoglutarate + O2
? + succinate + CO2
show the reaction diagram
-
-
-
?
(2S)-naringenin + 2-oxoglutarate + O2
(2R,3R)-dihydrokaempferol + succinate + CO2
show the reaction diagram
-
-
-
?
(S)-eriodictyol + 2-oxoglutarate + O2
(2R,3R)-dihydroquercetin + succinate + CO2
show the reaction diagram
-
-
-
?
3'-methoxy eriodictyol + 2-oxoglutarate + O2
? + succinate + CO2
show the reaction diagram
4'-methoxy eriodictyol + 2-oxoglutarate + O2
? + succinate + CO2
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
EDTA
0.2 M, complete loss of activity
EDTA
0.2 M, complete loss of activity
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0057
(S)-eriodictyol
pH 8.0, 37°C
0.0063 - 0.0578
(S)-eriodictyol
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
isoform OsF3H-2
UniProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
highest expression
Manually annotated by BRENDA team
highest expression
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
38700
x * 38700, calculated
36800
x * 36800, calculated
42700
x * 42700, calculated
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 38700, calculated
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression as glutathione S-transferase fusion protein
expression as glutathione S-transferase fusion protein
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Kim, J.H.; Lee, Y.J.; Kim, B.G.; Lim, Y.; Ahn, J.H.
Flavanone 3beta-hydroxylases from rice: key enzymes for favonol and anthocyanin biosynthesis
Mol. Cell
25
312-316
2008
Oryza sativa, Oryza sativa (Q7XR84), Oryza sativa (Q8W2X5)
Manually annotated by BRENDA team