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Requires Fe2+ and ascorbate. The enzyme has been characterized from the bacterium Pantoea ananatis.
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The expected taxonomic range for this enzyme is: Pantoea ananatis
, L-isoleucine 4'-dioxygenase, more
L-isoleucine + 2-oxoglutarate + O2 = 31-hydroxy-L-isoleucine + succinate + CO2
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