Information on EC 1.13.11.19 - cysteamine dioxygenase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.13.11.19
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RECOMMENDED NAME
GeneOntology No.
cysteamine dioxygenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2-aminoethanethiol + O2 = hypotaurine
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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redox reaction
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reduction
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
taurine biosynthesis II
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Taurine and hypotaurine metabolism
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Metabolic pathways
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SYSTEMATIC NAME
IUBMB Comments
2-aminoethanethiol:oxygen oxidoreductase
A non-heme iron protein that is involved in the biosynthesis of taurine. Requires catalytic amounts of a cofactor-like compound, such as sulfur, sufide, selenium or methylene blue for maximal activity. 3-Aminopropanethiol (homocysteamine) and 2-mercaptoethanol can also act as substrates, but glutathione, cysteine, and cysteine ethyl- and methyl esters are not good substrates [1,3].
CAS REGISTRY NUMBER
COMMENTARY hide
9033-41-4
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-aminoethanethiol + O2
hypotaurine
show the reaction diagram
3-mercaptopropionic acid + O2
3-sulfinopropionic acid
show the reaction diagram
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?
cysteamine + O2
hypotaurine
show the reaction diagram
homocysteamine + O2
2-aminopropansulfinic acid
show the reaction diagram
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-
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?
mercaptoethanol + O2
2-hydroxyethanesulfinic acid
show the reaction diagram
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?
N-acetylcysteamine + O2
N-acetylhypotaurine
show the reaction diagram
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?
pantetheine + O2
pantothenate + cysteamine
show the reaction diagram
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oxidized at less than 3% of the cysteamine-dependent rate
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?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2-aminoethanethiol + O2
hypotaurine
show the reaction diagram
cysteamine + O2
hypotaurine
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Cu
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1 atom of Cu per molecule of enzyme
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,3-dithiopropane
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1,4-dithiobutane
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2-mercaptoethanol
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inhibition at high concentration, activation at low concentration
8-hydroxyquinoline
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complete inhibition at 7.5 mM
alpha,alpha'-dipyridyl
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slight inhibition
cystamine
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cysteamine
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cysteine
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diethyldithiocarbamate
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70% inhibition at 10 mM
KCN
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60-70% inhibition at 10 mM
mercaptoethylguanidine
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noncompetitive to cysteamine
Neocuproine
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45-55% inhibition at 10 mM
o-phenanthroline
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45-55% inhibition at 10 mM
pantetheine
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S
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sulfide, elemental sulfur, elemental selenium or hydroxylamine required in catalytic amount, inhibition when added over a critical concentration (with the exception of hydroxylamine)
Salicylaldoxime
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slight inhibition
Se
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sulfide, elemental sulfur, elemental selenium or hydroxylamine required in catalytic amount, inhibition when added over a critical concentration (with the exception of hydroxylamine)
Sulfide
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sulfide, elemental sulfur, elemental selenium or hydroxylamine required in catalytic amount, inhibition when added over a critical concentration (with the exception of hydroxylamine)
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
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stimulation at low concentration, inhibition at high concentration
hydroxylamine
methylene blue
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cofactor-like compound
Sulfide
sulfur
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5
3-Mercaptopropionic acid
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0.001 - 0.62
cysteamine
0.83
homocysteamine
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1.2
mercaptoethanol
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0.71
N-acetylcysteamine
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0.1
O2
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.02
1,3-dithiopropane
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0.02
1,4-dithiobutane
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0.007
mercaptoethylguanidine
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.000021
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substrate: hypertaurine, kidney, activity is lower than in mice kidney
0.000026
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substrate: hypertaurine, liver, activity is lower than in mice liver
0.000037
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substrate: hypertaurine, kidney, higher activity in mice kidney than in rat kidney
0.000062
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substrate: hypertaurine, brain
0.000091
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substrate: hypertaurine, liver, higher activity in mice liver than in rat liver
2.8
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3 - 3.5
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 7.5
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7.8
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assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
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assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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Cysteamine levels in plasma of rats fed the cysteamine supplemented diet are significantly higher than those in tissues of rats fed basal diet. Rats fed the cysteamine-supplemented diet have no markedly elevated levels of hypotaurine in plasma.
Manually annotated by BRENDA team
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high activity
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50000
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2 * 50000 SDS-PAGE
52000
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2 * 52000, SDS-PAGE
64000
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2 * 64000, SDS-PAGE
83000
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sedimentation-diffusion equilibrium method
92000
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sedimentation equilibrium
96000
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gel filtration
100000
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method not mentioned
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
exhaustive dialysis against water: partial denaturation, dialysis against water brought to pH 7.5 with concentrated ammonia or against 0.01 M potassium phosphate buffer, pH 7.6, has no effect
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, up to 3 years
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0°C, 70% saturated ammonium sulfate, stable for months
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0°C, dialyzed and diluted solution, loss of 20% activity per week
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
overexpressed in HepG2/C3A cells
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relative expression level determination in cells grown in taurin-free or taurine-containing medium, overview
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
addition of taurine to cells grown in taurine-free medium has little effect on transcript levels of the biosynthetic pathway genes for cysteine dioxygenase (CDO), cysteine sulfinate decarboxylase (CSAD), or cysteamine dioxygenase (ADO). In contrast, supplementation with taurine causes a 30% reduction in transcript levels of the taurine transporter, TauT
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
H95A
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catalytically inactive
additional information
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Vanin 1-/- and +/+ mice