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Information on EC 1.1.1.83 - D-malate dehydrogenase (decarboxylating) and Organism(s) Escherichia coli and UniProt Accession P76251

for references in articles please use BRENDA:EC1.1.1.83
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This record set is specific for:
Escherichia coli
UNIPROT: P76251 not found.
Word Map
The taxonomic range for the selected organisms is: Escherichia coli
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
Synonyms
D-malate dehydrogenase, D-malic enzyme, dehydrogenase, D-malate (decarboxylating), dmlA, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D-malate dehydrogenase
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D-malic enzyme
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dehydrogenase, D-malate (decarboxylating)
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dmlA
292902
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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oxidative decarboxylation
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redox reaction
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reduction
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PATHWAY SOURCE
PATHWAYS
SYSTEMATIC NAME
IUBMB Comments
(R)-malate:NAD+ oxidoreductase (decarboxylating)
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CAS REGISTRY NUMBER
COMMENTARY hide
37250-20-7
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(R)-malate + NAD+
pyruvate + CO2 + NADH + H+
show the reaction diagram
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?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
divalent cation required
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SwissProt
Manually annotated by BRENDA team
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
addition of nitrate during anaerobic growth represses the expression of dmlA-lacZ about 2.2fold, but the expression is still higher than the expression under aerobic conditions. The presence of glucose during anaerobic growth represses dmlA expression to levels similar to those observed after nitrate addition, suggesting that there is some glucose repression (2.4fold)
in a wild-type background, D-malate and meso- and L-tartrate cause high levels of induction of dmlA-lacZ expression (up to 12.3fold). With L-malate, succinate, and D-tartrate there is only weak induction. Induction of dmlA encoding DmlA requires an intact dmlR gene, which encodes DmlR, a LysR-type transcriptional regulator; the expression of dmlA-lacZ at high levels is induced under anaerobic conditions in the presence of D-malate and is more than 5fold greater than the expression under aerobic conditions
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
analysis
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sequential fluorometric quantification of malic acid enantiomers in a single line flow-injection system using immobilized-enzyme reactors. An immobilized D-malate dehydrogenase (EC 1.1.1.83) reactor and an immobilized L-malate dehydrogenase (EC 1.1.1.40) reactor are introduced into the flowline in series. Sample and coenzyme (NAD+ or NADP+) are injected into the flow line by an open sandwich method. D-Malate is selectively oxidized by EC 1.1.1.83 when NAD+ is injected with a sample. When NADP+ is injected with a sample, L -malate is oxidized only by 1.1.1.40. NADH or NADPH produced by the immobilized-enzyme reactors is monitored fluorometrically at 455 nm
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Tsukatani, T.; Matsumoto, K.
Sequential fluorometric quantification of malic acid enantiomers by a single line flow-injection system using immobilized-enzyme reactors
Talanta
65
396-401
2005
Escherichia coli
Manually annotated by BRENDA team
Lukas, H.; Reimann, J.; Kim, O.B.; Grimpo, J.; Unden, G.
Regulation of aerobic and anaerobic D-malate metabolism of Escherichia coli by the LysR-type regulator DmlR (YeaT)
J. Bacteriol.
192
2503-2511
2010
Escherichia coli, Escherichia coli (P76251)
Manually annotated by BRENDA team
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