Any feedback?
Please rate this page
(ecexplorer.php)
(0/150)

BRENDA support

EC Explorer

EC 2.4.1.385 Details
EC number
2.4.1.385
Accepted name
sterol 27-β-glucosyltransferase
Reaction
UDP-α-D-glucose + a 27-hydroxysteroid = UDP + a sterol 27-β-D-glucoside
Systematic name
UDP-α-D-glucose:sterol 27-O-β-D-glucosyltransferase
Comment
The enzyme, isolated from the plant Withania somnifera (ashwagandha), transfers D-glucose to a β-hydroxyl group present at the C-27 position in sterols/withanolides, provided the substrate possesses a 17α-OH group. Natural substrates are 17α-hydroxywithaferin A, 27β-hydroxywithanone, and 5α,6β,17α,27β-tetrahydroxywithanolide. The enzyme's activity with withanolide A and withanolide U, which lack a 17α-hydroxyl group, suggests it may also be able to glucosylate the C-20 β-OH position, although this has not been verified yet. The enzyme does not glucosylate sterols at the C-3 position.
History
created 2021
EC Tree
2.4.1.3 created 1961, deleted 1972
2.4.1.6 created 1961, deleted 1972
2.4.1.42 created 1972, deleted 1984
2.4.1.51 created 1972, deleted 1984
2.4.1.55 created 1972, deleted 1982
2.4.1.59 created 1972, deleted 1984
2.4.1.61 created 1972, deleted 1984
2.4.1.72 created 1972, deleted 1976
2.4.1.75 created 1976, deleted 2005
2.4.1.76 created 1976, deleted 1984
2.4.1.77 created 1976, deleted 1984
2.4.1.84 created 1976, deleted 1984
2.4.1.89 created 1976, deleted 1984
2.4.1.93 created 1976, deleted 2004
2.4.1.98 created 1980, deleted 1984
2.4.1.107 created 1983, deleted 1984
2.4.1.108 created 1983, deleted 1984
2.4.1.124 created 1984, deleted 2002
2.4.1.151 created 1984, deleted 2002
2.4.1.169 created 1989, deleted 2003
2.4.1.200 created 1992, deleted 2004
2.4.1.204 created 1992, deleted 2003
2.4.1.233 created 2004, deleted 2005