2.1.1.B109: tRNA (cytosine32-N3)-methyltransferase
This is an abbreviated version!
For detailed information about tRNA (cytosine32-N3)-methyltransferase, go to the full flat file.
Word Map on EC 2.1.1.B109
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2.1.1.B109
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actin
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3-methylcytidine
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trnas
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actin-binding
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lifeact
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retrograde
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cables
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trnathr
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s-adenosylmethionine
- 2.1.1.B109
- actin
-
3-methylcytidine
- trnas
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actin-binding
-
lifeact
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retrograde
- cables
- trnathr
- s-adenosylmethionine
Reaction
Synonyms
Abp140p, methyltransferase-like protein 2B, METTL2B, Trm140p
ECTree
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Engineering
Engineering on EC 2.1.1.B109 - tRNA (cytosine32-N3)-methyltransferase
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D547A
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site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type
F481A
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site-directed mutagenesis, the mutant shows unaltered activity compared to the wild-type
K559A
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site-directed mutagenesis, the mutant shows unaltered activity compared to the wild-type
Y569A
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site-directed mutagenesis, the mutant shows unaltered activity compared to the wild-type
D547A
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site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type
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F481A
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site-directed mutagenesis, the mutant shows unaltered activity compared to the wild-type
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K559A
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site-directed mutagenesis, the mutant shows unaltered activity compared to the wild-type
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additional information
short-hairpin(sh)RNA knockdown of METTL2B mRNA in HeLa cells
additional information
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short-hairpin(sh)RNA knockdown of METTL2B mRNA in HeLa cells
additional information
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deletion of the conserved region near the C-terminus, i.e. delD602-Q621 and construction of the abp140DELTA knockout strain, expression of delD602-Q621 in the null mutant strain. Complementation of the yor239wD strain with plasmid-encoded ABP140 containing various mutations, in vitro reconstitution of m3C32 by recombinant Trm140p
additional information
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deletion of the conserved region near the C-terminus, i.e. delD602-Q621 and construction of the abp140DELTA knockout strain, expression of delD602-Q621 in the null mutant strain. Complementation of the yor239wD strain with plasmid-encoded ABP140 containing various mutations, in vitro reconstitution of m3C32 by recombinant Trm140p
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