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2.1.1.13: methionine synthase

This is an abbreviated version!
For detailed information about methionine synthase, go to the full flat file.

Word Map on EC 2.1.1.13

Reaction

5-methyltetrahydrofolate
+
L-homocysteine
=
tetrahydrofolate
 +
L-methionine

Synonyms

5-methyltetrahydrofolate homocysteine methyltransferase, 5-methyltetrahydrofolate-homocysteine methyltransferase, 5-methyltetrahydrofolate-homocysteine S-methyltransferase, 5-methyltetrahydrofolate-homocysteine transmethylase, B12 N5-methyltetrahydrofolate homocysteine methyltransferase, B12-dependent methionine synthase, cobalamin-dependent methionine synthase, folate-dependent methionine synthase, Met6p, MetH, methionine synthase, methionine synthetase, methyltetrahydrofolate-homocysteine vitamin B12 methyltransferase, methyltransferase, methyltetrahydrofolate-homocysteine, MetS, MS, MS1, MTR, N-methyltetrahydrofolate:L-homocysteine methyltransferase, N5-methyltetrahydrofolate methyltransferase, N5-methyltetrahydrofolate-homocysteine cobalamin methyltransferase, N5-methyltetrahydrofolic-homocysteine vitamin B12 transmethylase, tetrahydrofolate methyltransferase, tetrahydropteroylglutamate methyltransferase, tetrahydropteroylglutamic methyltransferase, vitamin B12 methyltransferase

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.13 methionine synthase

Activating Compound

Activating Compound on EC 2.1.1.13 - methionine synthase

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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
aquacob(III)alamin
-
the addition of 0.05 mM aquacob(III)alamin to the crude extract results in an about 13fold increase in activity which is not greatly affected by the addition of cytochrome P450 reductase or reductase domain of neuronal nitric oxide synthase
cadaverine
-
up to 40% stimulation at 1 mm
cyanocobalamin
K+
-
at 67 mM 1.4 fold activity
Li+
-
at 67 mM, 1.3fold activity
methionine synthase reductase
-
restores the activity of human methionine synthase through reductive methylation of methionine synthase-bound cob(II)alamin. Oxidized methionine synthase reductase does not reactivate methionine synthase, the presence of methionine synthase reductase along with AqCbl or MeCbl causes a dramatic increase in methionine synthase activity (7fold for AqCbl and 20fold for MeCbl) as compared to the cofactor alone
-
methylcob(III)alamin
-
the addition of 0.05 mM methylcob(III)alamin to the crude extract results in an about 6fold increase in activity which is not greatly affected by the addition of cytochrome P450 reductase or reductase domain of neuronal nitric oxide synthase
Na+
-
at 67 mM 1.5 fold activity
NADPH
-
reactivation of methionine synthase is dependent on NADPH concentration in a hyperbolic manner with maximum activity at about 0.2 mM
NH4+
-
at 67 mM 1.5 fold activity
putrescine
-
up to 50% stimulation at 1 mm
S-adenosyl-L-methionine
required
S-adenosylmethionine
-
at about 0.0001 mM
spermidine
-
up to 2.5 fold stimulation at 1 mm
spermine
-
up to 4 fold stimulation at 1 mm
TrisHCl
-
at 67 mM 1.2 fold activity
vitamin B12