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1.7.2.8: hydrazine dehydrogenase

This is an abbreviated version!
For detailed information about hydrazine dehydrogenase, go to the full flat file.

Word Map on EC 1.7.2.8

Reaction

hydrazine
+ 4 ferricytochrome c =
N2
 + 4 ferrocytochrome c

Synonyms

EC 1.7.99.8, HAO, HAOr-DH, HDH, hydrazine oxidoreductase, hydrazine-oxidizing enzyme, hydroxylamine oxidoreductase, hzoB

ECTree

     1 Oxidoreductases
         1.7 Acting on other nitrogenous compounds as donors
             1.7.2 With a cytochrome as acceptor
                1.7.2.8 hydrazine dehydrogenase

Purification

Purification on EC 1.7.2.8 - hydrazine dehydrogenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, Sepharose CL-6B column chromatography, Sephadex G-100 gel filtration, and DEAE-Sephacel column chromatography
-
hydroxylamine oxidoreductase (HAO) is purified from anammox sludge in which an anammox bacterium, strain KSU-1, is dominant
native enzyme 15fold by anion exchange chromatography and gel filtration
Ni-affinity column chromatography and Superdex 75 gel filtration
Ni-NTA column chromatography, hydroxyapatite column chromatography and Superose 6 gel filtration
-
partial, enzyme shows a deep orange-red colour
-
Q Sepharose column chromatography and hydroxyapatite column chromatography
-
Q Sepharose column chromatography, hydroxyapatite column chromatography and Superose 6 gel filtration
-