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Literature summary for 1.7.2.8 extracted from

  • Akram, M.; Dietl, A.; Mueller, M.; Barends, T.R.M.
    Purification of the key enzyme complexes of the anammox pathway from DEMON sludge (2021), Biopolymers, 112, e23428 .
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
hydroxylamine
-
Candidatus Brocadia fulgida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0076
-
hydrazine at pH 7.0 and 35°C Candidatus Brocadia fulgida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
200700
-
gel filtration Candidatus Brocadia fulgida

Organism

Organism UniProt Comment Textmining
Candidatus Brocadia fulgida
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Q Sepharose column chromatography, hydroxyapatite column chromatography and Superose 6 gel filtration Candidatus Brocadia fulgida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
hydrazine + 4 bovine heart ferricytochrome c
-
Candidatus Brocadia fulgida N2 + 4 bovine heart ferrocytochrome c
-
?

Subunits

Subunits Comment Organism
trimer 3 * 66900, calculated from amino acid sequence Candidatus Brocadia fulgida

Synonyms

Synonyms Comment Organism
HDH
-
Candidatus Brocadia fulgida

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.7
-
hydrazine at pH 7.0 and 35°C Candidatus Brocadia fulgida

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
220
-
hydrazine at pH 7.0 and 35°C Candidatus Brocadia fulgida