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1.14.14.175: ferruginol synthase

This is an abbreviated version!
For detailed information about ferruginol synthase, go to the full flat file.

Word Map on EC 1.14.14.175

Reaction

abieta-8,11,13-triene
+
[reduced NADPH-hemoprotein reductase]
 +
O2
=
ferruginol
 +
[oxidized NADPH-hemoprotein reductase]
 +
H2O

Synonyms

CYP76AH1, Cyp76AH22, Cyp76AH23, CYP76AH24, CYP76AH4, EC 1.14.13.190, FS1, FS2, miltiradiene oxidase, miltiradiene,NADPH:oxygen oxidoreductase (ferruginol forming)

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.14.175 ferruginol synthase

Engineering

Engineering on EC 1.14.14.175 - ferruginol synthase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D301E/N303S/V479F
mutation leads to a second oxidation of product to give 11-hydroxyferruginol, i.e. reaction of EC 1.14.14.60
F112L
-
mutant catalyzes ferruginol production in yeast 2 times more efficiently than the wild-type enzyme, while production of 11-hydroxy-ferruginol is abolished. mutant does not oxidize miltiradiene
V296L
-
mutant produces 1.5 times more ferruginol, but 8fold less 11-hydroxy-ferruginol and 20fold less 11beta-hydroxy-manoyl oxide than the wild-type enzyme. mutant does not oxidize miltiradiene
additional information