EC Number   |
Protein Variants |
Reference |
|---|
   3.4.24.69 | A308L |
the mutant shows 8.7% of wild type SNAP-25 cleavage activity |
754424 |
| 3.4.24.69 | A308V |
the mutant shows 86.4% of wild type SNAP-25 cleavage activity |
754424 |
| 3.4.24.69 | C134A |
site-directed mutagenesis, mutation of a binding site residue, the mutant shows reduced activity compared to the wild-type enzyme |
683671 |
| 3.4.24.69 | C423S |
inactive |
754839 |
| 3.4.24.69 | C461S |
the mutant shows similar levels of activity as the wild type enzyme |
754839 |
| 3.4.24.69 | C467S |
the mutant shows similar levels of activity as the wild type enzyme |
754839 |
| 3.4.24.69 | D130A |
site-directed mutagenesis, the mutant shows reduced activity and an altered ratio of activity in absence or presence of exogenous ZnCl2 compared to the wild-type enzyme |
684033 |
| 3.4.24.69 | D161A |
the mutant exhibits a 1000fold reduction of wild type activity |
755418 |
| 3.4.24.69 | D341A |
site-directed mutagenesis, a C2II component mutant, the mutant shows unaltered channel forming activity compared to the wild-type enzyme |
683678 |
| 3.4.24.69 | D342C |
site-directed mutagenesis, a C2II component mutant, the mutant shows unaltered channel forming activity compared to the wild-type enzyme |
683678 |
