Protein Variants | Comment | Organism |
---|---|---|
C134A | site-directed mutagenesis, mutation of a binding site residue, the mutant shows reduced activity compared to the wild-type enzyme | Clostridium botulinum |
D370A | site-directed mutagenesis, mutation of an S1' pocket residue, the mutant shows reduced activity compared to the wild-type enzyme | Clostridium botulinum |
D370R | site-directed mutagenesis, mutation of an S1' pocket residue, the mutant shows reduced activity compared to the wild-type enzyme | Clostridium botulinum |
E257A | site-directed mutagenesis, mutation of an S4' pocket residue, the mutant shows reduced activity compared to the wild-type enzyme | Clostridium botulinum |
E257K | site-directed mutagenesis, mutation of an S4' pocket residue, the mutant shows reduced activity compared to the wild-type enzyme | Clostridium botulinum |
F163A | site-directed mutagenesis, mutation of an S1' pocket residue, the mutant shows reduced activity compared to the wild-type enzyme | Clostridium botulinum |
F194A | site-directed mutagenesis, mutation of an S1' pocket residue, the mutant shows reduced activity compared to the wild-type enzyme | Clostridium botulinum |
F194A/T220A | site-directed mutagenesis, mutation of S1' pocket residues, the mutant shows reduced activity compared to the wild-type enzyme | Clostridium botulinum |
H269A | site-directed mutagenesis, the mutant shows unaltered activity compared to the wild-type enzyme | Clostridium botulinum |
I115A | site-directed mutagenesis, mutation of a binding site residue, the mutant shows reduced activity compared to the wild-type enzyme | Clostridium botulinum |
K41A | site-directed mutagenesis, mutation of a binding site residue, the mutant shows reduced activity compared to the wild-type enzyme | Clostridium botulinum |
L175A | site-directed mutagenesis, mutation of an S5 pocket residue, the mutant shows reduced activity compared to the wild-type enzyme | Clostridium botulinum |
L175A/R177A | site-directed mutagenesis, mutation of S5 pocket residues, the mutant shows reduced activity compared to the wild-type enzyme | Clostridium botulinum |
Q162A | site-directed mutagenesis, the mutant shows unaltered activity compared to the wild-type enzyme | Clostridium botulinum |
R177A | site-directed mutagenesis, mutation of an S5 pocket residue, the mutant shows reduced activity compared to the wild-type enzyme | Clostridium botulinum |
T176A | site-directed mutagenesis, mutation of an S5 pocket residue, the mutant shows reduced activity compared to the wild-type enzyme | Clostridium botulinum |
T220A | site-directed mutagenesis, mutation of an S1' pocket residue, the mutant shows reduced activity compared to the wild-type enzyme | Clostridium botulinum |
V129A | site-directed mutagenesis, mutation of a binding site residue, the mutant shows reduced activity compared to the wild-type enzyme | Clostridium botulinum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-Arginine hydroxamate | - |
Clostridium botulinum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0083 | - |
SNAP25 | pH 7.6, 37°C, recombinant mutant L175A/R177A | Clostridium botulinum | |
0.009 | - |
SNAP25 | pH 7.6, 37°C, recombinant mutant F194A | Clostridium botulinum | |
0.0092 | - |
SNAP25 | pH 7.6, 37°C, recombinant mutant F194A/T220A | Clostridium botulinum | |
0.011 | - |
SNAP25 | pH 7.6, 37°C, recombinant mutant D370R | Clostridium botulinum | |
0.011 | - |
SNAP25 | pH 7.6, 37°C, recombinant mutant L175A | Clostridium botulinum | |
0.012 | - |
SNAP25 | pH 7.6, 37°C, recombinant mutant F163A | Clostridium botulinum | |
0.012 | - |
SNAP25 | pH 7.6, 37°C, recombinant mutant R177A | Clostridium botulinum | |
0.014 | - |
SNAP25 | pH 7.6, 37°C, recombinant mutant D370A | Clostridium botulinum | |
0.014 | - |
SNAP25 | pH 7.6, 37°C, recombinant mutant T176A | Clostridium botulinum | |
0.016 | - |
SNAP25 | pH 7.6, 37°C, recombinant wild-type enzyme | Clostridium botulinum | |
0.016 | - |
SNAP25 | pH 7.6, 37°C, recombinant mutant H269A | Clostridium botulinum | |
0.017 | - |
SNAP25 | pH 7.6, 37°C, recombinant mutant Q162A | Clostridium botulinum | |
0.019 | - |
SNAP25 | pH 7.6, 37°C, recombinant mutant E257A | Clostridium botulinum | |
0.02 | - |
SNAP25 | pH 7.6, 37°C, recombinant mutant T220A | Clostridium botulinum | |
0.027 | - |
SNAP25 | pH 7.6, 37°C, recombinant mutant E257K | Clostridium botulinum | |
0.053 | - |
SNAP25 | pH 7.6, 37°C, recombinant mutant V129A | Clostridium botulinum | |
0.061 | - |
SNAP25 | pH 7.6, 37°C, recombinant mutant C134A | Clostridium botulinum | |
0.095 | - |
SNAP25 | pH 7.6, 37°C, recombinant mutant I115A | Clostridium botulinum | |
0.098 | - |
SNAP25 | pH 7.6, 37°C, recombinant mutant K41A | Clostridium botulinum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
SNAP25 + H2O | Clostridium botulinum | i.e. soluble N-ethylmaleimide-sensitive factor attachment protein 25, the enzyme cleaves SNARE proteins, i.e. SNAP receptor proteins, to elicit flaccid paralysis by inhibiting neurotransmitter-carrying vesicle fusion to the plasma membrane of peripheral neurons, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium botulinum | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
SNAP25 + H2O | i.e. soluble N-ethylmaleimide-sensitive factor attachment protein 25, the enzyme cleaves SNARE proteins, i.e. SNAP receptor proteins, to elicit flaccid paralysis by inhibiting neurotransmitter-carrying vesicle fusion to the plasma membrane of peripheral neurons, overview | Clostridium botulinum | ? | - |
? | |
SNAP25 + H2O | i.e. soluble N-ethylmaleimide-sensitive factor attachment protein 25, recombinant GST-tagged wild-type and mutant D193A, R198A, R198E, and I171A substrates, full-length and truncated substrate, SNAP25 initially binds along the belt region of BoNT/A, which aligns the P5 residue to the S5 pocket at the periphery of the active site, binding site structures, reaction mechanism, molecular modeling of the LC/A active site domain, overview | Clostridium botulinum | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BoNT/A | - |
Clostridium botulinum |
botulinum neurotoxin serotype A | - |
Clostridium botulinum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Clostridium botulinum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
SNAP25 | pH 7.6, 37°C, recombinant mutant D370R | Clostridium botulinum | |
0.1 | - |
SNAP25 | pH 7.6, 37°C, recombinant mutant D370A | Clostridium botulinum | |
0.1 | - |
SNAP25 | pH 7.6, 37°C, recombinant mutant E257K | Clostridium botulinum | |
0.19 | - |
SNAP25 | pH 7.6, 37°C, recombinant mutant F194A/T220A | Clostridium botulinum | |
0.2 | - |
SNAP25 | pH 7.6, 37°C, recombinant mutant F194A | Clostridium botulinum | |
0.3 | - |
SNAP25 | pH 7.6, 37°C, recombinant mutant T220A | Clostridium botulinum | |
0.4 | - |
SNAP25 | pH 7.6, 37°C, recombinant mutant F163A | Clostridium botulinum | |
0.7 | - |
SNAP25 | pH 7.6, 37°C, recombinant mutant L175A | Clostridium botulinum | |
0.8 | - |
SNAP25 | pH 7.6, 37°C, recombinant mutant L175A/R177A | Clostridium botulinum | |
1.1 | - |
SNAP25 | pH 7.6, 37°C, recombinant mutants R177A and E257A | Clostridium botulinum | |
1.2 | - |
SNAP25 | pH 7.6, 37°C, recombinant mutant T176A | Clostridium botulinum | |
11 | - |
SNAP25 | pH 7.6, 37°C, recombinant mutants I115A and C134A | Clostridium botulinum | |
12 | - |
SNAP25 | pH 7.6, 37°C, recombinant mutants K41A and V129A | Clostridium botulinum | |
58 | - |
SNAP25 | pH 7.6, 37°C, recombinant mutant H269A | Clostridium botulinum | |
60 | - |
SNAP25 | pH 7.6, 37°C, recombinant wild-type enzyme | Clostridium botulinum | |
63 | - |
SNAP25 | pH 7.6, 37°C, recombinant mutant Q162A | Clostridium botulinum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Clostridium botulinum |