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Literature summary for 3.4.24.69 extracted from

  • Zhang, S.; Masuyer, G.; Zhang, J.; Shen, Y.; Lundin, D.; Henriksson, L.; Miyashita, S.I.; Martinez-Carranza, M.; Dong, M.; Stenmark, P.
    Identification and characterization of a novel botulinum neurotoxin (2017), Nat. Commun., 8, 14130 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.24.69

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Clostridium botulinum

Protein Variants

Protein Variants Comment Organism
C423S inactive Clostridium botulinum
C461S the mutant shows similar levels of activity as the wild type enzyme Clostridium botulinum
C467S the mutant shows similar levels of activity as the wild type enzyme Clostridium botulinum

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ dependent on Clostridium botulinum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
150000
-
 gel filtration Clostridium botulinum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
vesicle-associated membrane protein-1 + H2O Clostridium botulinum cleavage at Arg66-Ala67 ?
-
 ?
vesicle-associated membrane protein-2 + H2O Clostridium botulinum cleavage at Arg66-Ala67 ?
-
 ?
vesicle-associated membrane protein-3 + H2O Clostridium botulinum cleavage at Arg66-Ala67 ?
-
 ?
vesicle-associated membrane protein-4 + H2O Clostridium botulinum cleavage at Lys87-Ser88 ?
-
 ?
vesicle-associated membrane protein-5 + H2O Clostridium botulinum cleavage at Arg40-Ser41 ?
-
 ?
Ykt6 + H2O Clostridium botulinum cleavage at Lys173-Ser174 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Clostridium botulinum P0DPK1
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Clostridium botulinum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with vesicle-associated membrane protein-7, vesicle-associated membrane protein-8, syntaxin 1, SNAP-25 and Sec22b Clostridium botulinum ?
-
 ?
vesicle-associated membrane protein-1 + H2O cleavage at Arg66-Ala67 Clostridium botulinum ?
-
 ?
vesicle-associated membrane protein-2 + H2O cleavage at Arg66-Ala67 Clostridium botulinum ?
-
 ?
vesicle-associated membrane protein-3 + H2O cleavage at Arg66-Ala67 Clostridium botulinum ?
-
 ?
vesicle-associated membrane protein-4 + H2O cleavage at Lys87-Ser88 Clostridium botulinum ?
-
 ?
vesicle-associated membrane protein-5 + H2O cleavage at Arg40-Ser41 Clostridium botulinum ?
-
 ?
Ykt6 + H2O cleavage at Lys173-Ser174 Clostridium botulinum ?
-
 ?

Synonyms

Synonyms Comment Organism
BoNT/X
-
 Clostridium botulinum
Botulinum neurotoxin
-
 Clostridium botulinum