EC Number   |
Protein Variants |
Reference |
|---|
    3.2.1.3 | A246C |
site-directed mutagenesis, the mutant has a strong additive thermostabilizing effect |
-, 717114 |
| 3.2.1.3 | A246C |
the T50-value is enhanced by 4°C to 73°C. Compared to wild-type enzyme, the mutant is twice as active at 66°C but half as active at 45°C |
136231 |
| 3.2.1.3 | A276C/S347C |
101% of wild-type kcat |
657229 |
| 3.2.1.3 | A276C/S347C/S298C |
107% of wild-type kcat |
657229 |
| 3.2.1.3 | C320A |
barely improved thermostability or altered activity |
136231 |
| 3.2.1.3 | D20C/A27C/S30P/G137A |
site-directed mutagenesis, the mutant, designated THS8, is highly thermotolerant with increased stability at 80°C compared to the wild-type enzyme |
682864 |
| 3.2.1.3 | D71N |
increase in thermosability at 65 and 75°C |
657217 |
| 3.2.1.3 | DELTA439-441 |
increase in thermosability at 65 and 75°C |
657217 |
| 3.2.1.3 | E180Q |
active site mutant. For inhibitor acarbose, a rapid binding event is apparently intersected by a slower secondary binding event. Mutant shows a dramatically higher Kd value for acarbose |
729537 |
| 3.2.1.3 | E180Q |
mutant of G1, 48% of wild-type kcat for maltose, 88% of kcat for maltotriose |
654806 |
