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Literature summary for 3.2.1.3 extracted from

  • Wang, Y.; Fuchs, E.; da Silva, R.; McDaniel, A.; Seibel, J.; Ford, C.
    Improvement of Aspergillus niger glucoamylase thermostability by directed evolution (2006), Starch Staerke, 58, 501-508.
No PubMed abstract available
Search for more literature for 3.2.1.3

Application

Application Comment Organism
industry glucoamylase is used industrially to catalyze the hydrolysis of alpha-1,4-glycosidic bonds to release beta-D-glucose from the nonreducing ends of starch or oligosaccharides Aspergillus niger

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and anaylsis of wild-type and mutant enzymes, expression of wild-type and mutant enzymes in Saccharomyces cerevisiae strain C468 using vector YEpPM18 Aspergillus niger

Protein Variants

Protein Variants Comment Organism
D20C/A27C/S30P/G137A site-directed mutagenesis, the mutant, designated THS8, is highly thermotolerant with increased stability at 80°C compared to the wild-type enzyme Aspergillus niger
H391Y random mutagenesis, the mutant shows increased thermotolerance compared to the wild-type enzyme Aspergillus niger
additional information random mutagenesis and mutant screening by plate thermostability assay for increased thermotolerance at 65-80°C, overview Aspergillus niger
T290A random mutagenesis, the mutant shows increased thermotolerance compared to the wild-type enzyme Aspergillus niger
T62A random mutagenesis, the mutant shows increased thermotolerance compared to the wild-type enzyme Aspergillus niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.76
-
 maltose pH 4.5, 35°C, recombinant mutant T62A Aspergillus niger
0.78
-
 maltose pH 4.5, 35°C, recombinant mutant T290A Aspergillus niger
0.82
-
 maltose pH 4.5, 35°C, recombinant mutant H391Y Aspergillus niger
0.83
-
 maltose pH 4.5, 35°C, recombinant wild-type enzyme Aspergillus niger
0.88
-
 maltose pH 4.5, 35°C, recombinant mutant S30P/T62A/H391Y Aspergillus niger
0.89
-
 maltose pH 4.5, 35°C, recombinant mutant S119P Aspergillus niger
1
-
 maltose pH 4.5, 35°C, recombinant mutant D20C/A27C/S30P/G137A Aspergillus niger
1.16
-
 maltose pH 4.5, 35°C, recombinant mutant S30P/T290A/H391Y Aspergillus niger
1.19
-
 maltose pH 4.5, 35°C, recombinant mutant S30P/H391Y Aspergillus niger
1.35
-
 maltose pH 4.5, 35°C, recombinant mutant S30P/T290A Aspergillus niger

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
starch + H2O Aspergillus niger
-
 beta-D-glucose + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Aspergillus niger

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
13.3
-
 recombinant mutant S30P/T62A/H391Y Aspergillus niger
13.6
-
 recombinant mutant T62A Aspergillus niger
14
-
 recombinant mutant T290A Aspergillus niger
14.2
-
 recombinant wild-type enzyme Aspergillus niger
16.8
-
 recombinant mutant S119P Aspergillus niger
16.9
-
 recombinant mutant D20C/A27C/S30P/G137A Aspergillus niger
17.7
-
 recombinant mutant H391Y Aspergillus niger
17.9
-
 recombinant mutant S30P/T290A Aspergillus niger
18.1
-
 recombinant mutant S30P/T290A/H391Y Aspergillus niger
19.5
-
 recombinant mutant S30P/H391Y Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
maltose + H2O
-
 Aspergillus niger beta-D-glucose + D-glucose
-
 ?
starch + H2O
-
 Aspergillus niger beta-D-glucose + ?
-
 ?

Synonyms

Synonyms Comment Organism
glucoamylase
-
 Aspergillus niger

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 assay at Aspergillus niger

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70 80 irreversible thermoinactivation kinetic analysis of recombinant wild-type and mutant enzymes, overview Aspergillus niger

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.03 0.55 maltose pH 4.5, 35°C, recombinant mutant T290A Aspergillus niger
0.031 0.51 maltose pH 4.5, 35°C, recombinant wild-type enzyme Aspergillus niger
0.04 1.97 maltose pH 4.5, 35°C, recombinant mutant S30P/T62A/H391Y Aspergillus niger
0.04 1.97 maltose pH 4.5, 35°C, recombinant mutant T62A Aspergillus niger
0.052 2.1 maltose pH 4.5, 35°C, recombinant mutant S30P/H391Y Aspergillus niger
0.057 0.65 maltose pH 4.5, 35°C, recombinant mutant D20C/A27C/S30P/G137A Aspergillus niger
0.5
-
 maltose pH 4.5, 35°C, recombinant mutant S119P Aspergillus niger
7.35
-
 maltose pH 4.5, 35°C, recombinant mutant S30P/T290A Aspergillus niger
7.46
-
 maltose pH 4.5, 35°C, recombinant mutant T290A Aspergillus niger
7.55
-
 maltose pH 4.5, 35°C, recombinant wild-type enzyme Aspergillus niger
8.99
-
 maltose pH 4.5, 35°C, recombinant mutant T62A Aspergillus niger
9.3
-
 maltose pH 4.5, 35°C, recombinant mutant S30P/T290A/H391Y Aspergillus niger
9.42
-
 maltose pH 4.5, 35°C, recombinant mutant S30P/T62A/H391Y Aspergillus niger
10.13
-
 maltose pH 4.5, 35°C, recombinant mutant S30P/H391Y Aspergillus niger
10.5
-
 maltose pH 4.5, 35°C, recombinant mutant H391Y Aspergillus niger
11.32
-
 maltose pH 4.5, 35°C, recombinant mutant D20C/A27C/S30P/G137A Aspergillus niger
13.31
-
 maltose pH 4.5, 35°C, recombinant mutant S119P Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.4 4.5 assay at Aspergillus niger