Literature summary for 3.2.1.3 extracted from

Liu, H.L.; Doleyres, Y.; Coutinho, P.M.; Ford, C.; Reilly, P.J.
Replacement and deletion mutations in the catalytic domain and belt region of Aspergillus awamori glucoamylase to enhance thermostability (2000), Protein Eng., 13, 655-659.

Search for more literature for 3.2.1.3

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Saccharomyces cerevisiae	Aspergillus awamori

Protein Variants

Protein Variants	Comment	Organism
D71N	increase in thermosability at 65 and 75°C	Aspergillus awamori
DELTA439-441	increase in thermosability at 65 and 75°C	Aspergillus awamori
G447S	increase in thermosability at 65 and 75°C	Aspergillus awamori
Q409P	increase in thermosability at 65 and 75°C	Aspergillus awamori

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.72	-	maltose	45°C, pH 4.4	Aspergillus awamori

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
starch + H2O	Aspergillus awamori	-	beta-D-glucose + ?	-	?

Organism

Organism	UniProt	Comment	Textmining
Aspergillus awamori	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	-	Aspergillus awamori

Purification (Commentary)

Purification (Comment)	Organism
recombinant glucoamylase	Aspergillus awamori

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
20.6	-	-	Aspergillus awamori

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
maltose + H2O	-	Aspergillus awamori	beta-D-glucose + D-glucose	-	?
starch + H2O	-	Aspergillus awamori	beta-D-glucose + ?	-	?

Synonyms

Synonyms	Comment	Organism
GA	-	Aspergillus awamori

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
8.67	-	maltose	45°C, pH 4.4	Aspergillus awamori

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Release 2023.1 (January 2023)