EC Number   |
Protein Variants |
Reference |
|---|
   3.1.4.11 | C1094S |
NorpA mutant with slowed activation and deactivation |
653442 |
| 3.1.4.11 | D114A |
catalytically inactive Isc1 mutant |
-, 715041 |
| 3.1.4.11 | D161R |
the mutant shows 10% and 5% hydrolyzing activity for 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate and 1-phosphatidyl-1D-myo-inositol 4-phosphate, respectively, compared to the wild type enzyme |
751706 |
| 3.1.4.11 | F237A |
site directed mutagenesis |
691015 |
| 3.1.4.11 | F237W |
point mutation of surface hydrophobic residues |
691015 |
| 3.1.4.11 | G48A |
single PI-PLC mutant, site-directed mutagenesis |
690951 |
| 3.1.4.11 | H129A |
the mutant shows less than 22% and 6% hydrolyzing activity for 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate and 1-phosphatidyl-1D-myo-inositol 4-phosphate, respectively, compared to the wild type enzyme |
751706 |
| 3.1.4.11 | H503A |
mutant enzyme shows reduced affinity for phosphoinositide binding and reduced hydrolysis of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate |
669162 |
| 3.1.4.11 | I43A |
single PI-PLC mutant, site-directed mutagenesis |
690951 |
| 3.1.4.11 | I43W |
single PI-PLC mutant, site-directed mutagenesis |
690951 |
