Information on EC 3.1.4.11 - phosphoinositide phospholipase C

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY
3.1.4.11
-
RECOMMENDED NAME
GeneOntology No.
phosphoinositide phospholipase C
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
-
-
-
-
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
mechanism
-
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
mechanism
-
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
biochemical relationship between PLC and heterotrimeric G proteins during NFs signaling in legumes
-
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
kinetic and catalytic reaction mechanism, overview
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of phosphoric ester
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
D-myo-inositol (1,4,5)-trisphosphate biosynthesis
-
-
D-myo-inositol-5-phosphate metabolism
-
-
Inositol phosphate metabolism
-
-
Metabolic pathways
-
-
phosphatidate metabolism, as a signaling molecule
-
-
phospholipases
-
-
SYSTEMATIC NAME
IUBMB Comments
1-phosphatidyl-1D-myo-inositol-4,5-bisphosphate inositoltrisphosphohydrolase
These enzymes form some of the cyclic phosphate Ins(cyclic1,2)P(4,5)P2 as well as Ins(1,4,5)P3. They show activity towards phosphatidylinositol, i.e., the activity of EC 4.6.1.13, phosphatidylinositol diacylglycerol-lyase, in vitro at high [Ca2+]. Four beta-isoforms regulated by G-proteins, two gamma-forms regulated by tyrosine kinases, four delta-forms regulated at least in part by calcium and an epsilon-form, probably regulated by the oncogene ras, have been found.
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
1-phosphatidyl-D-myo-inositol 4,5-bisphosphate inositoltrisphosphohydrolase
-
-
-
-
1-phosphatidyl-D-myo-inositol-4,5-bisphosphate inositoltrisphosphohydrolase
-
-
1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase
-
-
-
-
1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase beta-1
Q9Z1B3
-
AtPLC1
Q39032
shown in vitro phosphoinositide-specific phospholipase C activities
AtPLC2
Q39033
shown in vitro phosphoinositide-specific phospholipase C activities
AtPLC3
Q56W08
shown in vitro phosphoinositide-specific phospholipase C activities
AtPLC4
Q944C1
-
AtPLC4
Q944C1
shown in vitro phosphoinositide-specific phospholipase C activities
AtPLC5
Q944C2
shown in vitro phosphoinositide-specific phospholipase C activities
AtPLC6
Q8GV43
contain necessary domains for active phosphoinositide-specific phospholipase C and is most likely active
AtPLC7
Q9LY51
contain necessary domains for active phosphoinositide-specific phospholipase C and is most likely active
AtPLC8
Q9STZ3
is unlikely to be enzymatically active
AtPLC9
Q6NMA7
is unlikely to be enzymatically active
Ca2 +-dependent phosphatidylinositol phosphodiesterase
-
-
calcium-ion-dependent phosphatidylinositol phosphodiesterase
-
-
cyclic phosphodiesterase
P34024
-
DsPLC2
Q9FSW1
-
endogenous phospholipase C
-
-
GPI-PLC
-
-
GPI-specific phospholipase C
-
-
hPLC-eta
Q4KWH8
-
inositol phosphosphingolipid-phospholipase C
-
-
inositol phosphosphingolipid-phospholipase C
Cryptococcus neoformans H99
-
-
-
IPC-PLC
Cryptococcus neoformans H99
-
-
-
Isc1
Cryptococcus neoformans H99
-
-
-
low molecular weight PI-PLC
-
-
monophosphatidylinositol inositolphosphohydrolase
-
-
mPLC-eta
Q4KWH5
-
myocardial phospholipase C
-
type I, II, III, and IV
No receptor potential A protein
-
-
-
-
NorpA
-
Drosophila sp. PLC-beta homolog
PH-PLCdelta1
-
-
phosphatidylinosite-specific phospholipase C
Q9P212
-
phosphatidylinositide-specific phospholipase C
Q6SA76, Q6TM09, Q6TM10
-
phosphatidylinositol -phospholipase C
-
-
phosphatidylinositol 4,5 bisphosphate-phospholipase C
-
-
phosphatidylinositol 4,5-bisphosphate phosphodiesterase
-
-
-
-
phosphatidylinositol phosphodiesterase
-
-
phosphatidylinositol phosphodiesterase
-
-
phosphatidylinositol phosphodiesterase
Mus musculus C57BL
-
-
-
phosphatidylinositol phosphodiesterase
-
-
phosphatidylinositol phosphodiesterase
-
-
phosphatidylinositol phosphodiesterase
-
-
phosphatidylinositol phosphodiesterase
-
phospholipase C-type
phosphatidylinositol phosphodiesterase
Rattus norvegicus Sprague-Dawley
-
-
-
phosphatidylinositol phosphodiesterase
-
-
phosphatidylinositol phospholipase C
-
-
phosphatidylinositol specific phospholipase C
-
-
phosphatidylinositol-4,5-bisphosphate phosphodiesterase
-
-
-
-
phosphatidylinositol-4,5-bisphosphate phospholipase C
-
-
-
-
phosphatidylinositol-4,5-bisphosphate-specific phospholipase C
O24297
-
phosphatidylinositol-phosphodiesterase
-
-
phosphatidylinositol-phosphodiesterase
-
-
phosphatidylinositol-specific phospholipase
P34024
-
phosphatidylinositol-specific phospholipase C
-
-
phosphatidylinositol-specific phospholipase C
-
-
phosphatidylinositol-specific phospholipase C
B2LSE7
-
phosphatidylinositol-specific phospholipase C
B4XX94, B4XX95
-
phosphatidylinositol-specific phospholipase C
-
-
phosphatidylinositol-specific phospholipase C
Q766D4
-
phosphatidylinositol-specific phospholipase C
-
-
phosphatidylinositol-specific phospholipase C
-
-
phosphatidylinositol-specific phospholipase C
Q6QJ78
-
phosphatidylinositol-specific phospholipase C-beta
Q00722
-
phosphatidylinositol-specific phospholipase C1
Q2YGU0
-
phosphatidylinositol-specific phospholipase C2
Q2YGU1
-
phosphatidylinositol-specific phospholipase-C
-
-
phosphatidylinositol-specific phospholipases C
-
-
phosphodiesterase
P34024
-
phosphodiesterase
-
-
phosphodiesterase, triphosphoinositide
-
-
-
-
phosphoinositidase C
-
-
-
-
phosphoinositide phospholipase C
Q9Z1B3
-
phosphoinositide specific phospholipase C
-
-
phosphoinositide-phospholipase C
Q15147
-
phosphoinositide-phospholipase C beta1
P19174, Q9NQ66
-
phosphoinositide-specific phospholipase
-
-
phosphoinositide-specific phospholipase C
Q39032, Q39033, Q56W08, Q6NMA7, Q8GV43, Q944C1, Q944C2, Q9LY51, Q9STZ3
-
phosphoinositide-specific phospholipase C
-
-
phosphoinositide-specific phospholipase C
-
-
phosphoinositide-specific phospholipase C
-
-
phosphoinositide-specific phospholipase C
-
-
phosphoinositide-specific phospholipase C
Q9Z1B3
-
phosphoinositide-specific phospholipase C
-
-
phosphoinositide-specific phospholipase C
-
-
phosphoinositide-specific phospholipase C
P10687
-
phosphoinositide-specific phospholipase C
P32383
-
phosphoinositide-specific phospholipase C
-
-
phosphoinositide-specific phospholipase C
-
-
phosphoinositide-specific phospholipase C beta1
Q9NQ66
-
phosphoinositide-specific phospholipase C beta1
Q9Z1B3
-
phosphoinositide-specific phospholipase C beta1
P10687
-
phosphoinositide-specific phospholipase C-zeta
-
-
phosphoinositide-specific phospholipase-Cgamma
-
-
phosphoinositide-specific phospholipases C
-
-
phospholipase C
-
-
-
-
phospholipase C
-
-
phospholipase C
-
type I, II, III, and IV
phospholipase C
-
-
phospholipase C
-
-
phospholipase C
-
-
phospholipase C
-
-
phospholipase C
P10686, P10687, P10688, Q99JE6
-
phospholipase C
P32383
-
phospholipase C 1
Q6QJ78
-
phospholipase C beta1
Q9NQ66
-
phospholipase C beta1
Q9Z1B3
-
phospholipase C beta1
-
-
phospholipase C beta3
Q01970
-
phospholipase C epsilon
Q9P212
-
phospholipase C epsilon
-
-
phospholipase C-beta 2
Q00722
-
phospholipase C-beta-1
Q9Z1B3
-
phospholipase C-beta1
-
-
phospholipase C-beta2
Q00722
-
phospholipase C-beta3
-
-
phospholipase C-delta-1
Q8R3B1
-
phospholipase C-delta1
P51178
-
phospholipase C-delta1
Q7T274
-
phospholipase C-epsilon
-
-
phospholipase C-epsilon
-
-
phospholipase C-eta1a
Q4KWH8
-
phospholipase C-eta1a
Q4KWH5
-
phospholipase C-eta1b
Q4KWH8
-
phospholipase C-eta1c
Q4KWH5
-
phospholipase C-gamma1
P19174
-
phospholipase C-gamma2
-
-
phospholipase C-gamma2
P16885
-
phospholipase C-gamma2
-
-
phospholipase C-gamma2
Q8CIH5
-
phospholipase C-like phosphodiesterase
-
-
phospholipase Cbeta1
P10687
-
phospholipase Cepsilon
-
-
phospholipase Cepsilon
Q8K4S1
-
phospholipase Cgamma1
-
-
phospholipase Cgamma1
-
-
phospholipase Cgamma2
-
-
phospholipase Cgamma2
Q8CIH5
-
phospholipase Cgamma2
Mus musculus C57BL/6
-
-
-
phosphotidylinositol 4,5-bisphosphate-specific phospholipase C
-
-
-
-
PI-PLC
Q39032, Q39033, Q56W08, Q6NMA7, Q8GV43, Q944C1, Q944C2, Q9LY51, Q9STZ3
-
PI-PLC
B2LSE7
-
PI-PLC
-
-
PI-PLC
Q9NQ66
-
PI-PLC
Q9P212
-
PI-PLC
Q2YGU0, Q2YGU1
-
PI-PLC
P34024
-
PI-PLC
Q9Z1B3
-
PI-PLC
Q766D4
-
PI-PLC
Q6SA76, Q6TM09, Q6TM10
-
PI-PLC
Q6QJ78
-
PI-PLC beta 1
Q9Z1B3
-
PI-PLC beta 1
P10687
-
PI-PLC beta1
-
-
PI-PLC beta1
P10687
-
PI-PLC beta3
-
-
PI-PLC beta3
Q99JE6
-
PI-PLC beta4
-
-
PI-PLC beta4
Q9QW07
-
PI-PLC delta1
P10688
-
PI-PLC delta3
-
-
PI-PLC delta4
Q62711
-
PI-PLC epsilon
Q99P84
-
PI-PLC gamma1
-
-
PI-PLC gamma1
P10686
-
PI-PLC gamma2
-
-
PI-PLCbeta
-
-
PI-PLCbeta1
Q9NQ66
-
PI-PLCbeta4
Q15147
-
PI-PLCc
-
-
PI-PLCgamma1
P19174
-
PI-PLCm
-
-
PI-specific phospholipase C
Q9NQ66
-
PI-specific phospholipase C
Q9Z1B3
-
PIC
-
-
-
-
PIP(2)-specific-phospholipase
-
-
PIP2 PDE
-
-
-
-
PIP2 phosphodiesterase
-
-
-
-
PIP2-phospholipase C
-
-
PIP2-PLC
-
-
PIP2-PLC
-
-
PIP2-PLC
O24297
-
PIP2-specific phospholipase C
O24297
-
PIPLC
-
-
-
-
PL-C
-
-
PLC
-
-
-
-
PLC beta1
-
-
PLC beta1
-
-
PLC beta1
P10687
-
PLC beta1b
-
-
PLC beta3
Q99JE6
-
PLC delta1
P10688
-
PLC epsilon
Q9P212
-
PLC epsilon1a
Q9P212
PLC epsilon splice variant
PLC epsilon1b
Q9P212
PLC epsilon splice variant
PLC gamma1
-
-
PLC gamma1
P10686
-
PLC-148
-
-
-
-
PLC-154
-
-
-
-
PLC-154
Q9Z1B3
-
PLC-21
-
Drosophila sp. PLC-beta homolog
PLC-85
-
-
-
-
PLC-beta
-
-
PLC-beta-1
Q9Z1B3
-
PLC-beta1b
-
-
PLC-beta2
-
-
PLC-beta2
Q00722
-
PLC-delta
-
-
PLC-delta-1
Q8R3B1
-
PLC-delta1
-
-
PLC-delta1
-
-
PLC-epsilon
-
-
PLC-epsilon
-
-
PLC-epsilon
-
-
PLC-eta
-
-
PLC-gamma
-
-
PLC-gamma
-
-
PLC-gamma1
P19174
phospho-nephrin-binding protein
PLC-gamma2
P16885
-
PLC-gamma2
Q8CIH5
-
PLC-I
-
-
PLC-I
Q9Z1B3
-
PLC-II
-
-
PLC-zeta
-
-
PLC-zeta
-
-
PLC-zeta
Q8K4D7
-
PLCbeta1
Q9NQ66
-
PLCbeta1
Q9Z1B3
-
PLCbeta1
-
-
PLCbeta1
P10687
-
PLCbeta1
P10687
two splice variants, PLCbeta1a and PLCbeta1b
PLCdelta1
-
-
PLCdelta1
Q8R3B1
-
PLCdelta1
-
-
PLCdeta1
-
-
PLCepsilon
-
splice variants PLCepsilon1a and PLCepsilon1b
PLCeta2
A2AP18
-
PLCgamma
-
-
PLCgamma
-
-
PLCgamma
-
-
PLCgamma1
-
-
PLCgamma1
-
-
PLCgamma1
-
-
PLCgamma1
Mus musculus C57BL/6
-
-
-
PLCgamma1
-
-
PLCgamma2
-
-
PLCgamma2
Q8CIH5
-
PLCgamma2
Q8CIH5
mediates RANKL-stimulated lymph node organogenesis and osteoclastogenesis
PLCgamma2
Mus musculus C57BL/6
-
-
-
pleckstrin homology-phospholipase C-delta1
-
-
polyphosphoinositide phospholipase C
-
-
-
-
PsPLC
O24297
-
PtdIns phosphodiesterase
-
-
PtdIns phosphodiesterase
Mus musculus C57BL
-
-
-
PtdIns phosphodiesterase
-
-
PtdIns phosphodiesterase
Rattus norvegicus Sprague-Dawley
-
-
-
PtdIns(4,5)P2-directed phospholipase C
-
-
-
-
PtdIns(4,5)P2-PLC
-
-
suPLCbeta
-
-
TcPI-PLC
-
-
TcPI-PLC
Q9TZN8
PI-PLC of Trypanosoma cruzi, similar to mamalian delta-type PI-PLCs
TgPI-PLC
Q3ZTU7
-
triphosphoinositide phosphodiesterase
-
-
-
-
Vr-PLC1
Q6TM09
-
Vr-PLC2
Q6SA76
-
Vr-PLC3
Q6TM10
-
Vr-PLC3 protein
-
-
ZmPLC1
Q6QJ78
transgenic maize
monophosphatidylinositol phosphodiesterase
-
-
additional information
-
four major types of PLC: beta, gamma, delta and epsilon
additional information
-
four PLC subfamilies: beta, gamma, delta and epsilon
additional information
-
ten mammalian isoenzymes, grouped into three families: PLCbeta, PLCgamma and PLCdelta
additional information
-
four major types of PLC: beta, gamma, delta and epsilon
additional information
Q99P84
four PLC classes: beta, gamma and delta, which are differentially regulated by heterotrimeric G-proteins, tyrosine kinases and calcium, and epsilon
additional information
Rattus norvegicus PLCepsilon
Q99P84
four PLC classes: beta, gamma and delta, which are differentially regulated by heterotrimeric G-proteins, tyrosine kinases and calcium, and epsilon
-
CAS REGISTRY NUMBER
COMMENTARY
37213-51-7
-
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
4 weeks old, AtPLC2
SwissProt
Manually annotated by BRENDA team
ATPLC1; ecotype Columbia-0
UniProt
Manually annotated by BRENDA team
AtPLC1S, AtPLC2, delta-related isotypes
-
-
Manually annotated by BRENDA team
ATPLC2; ecotype Columbia-0
SwissProt
Manually annotated by BRENDA team
ATPLC3; ecotype Columbia-0
UniProt
Manually annotated by BRENDA team
ATPLC4; ecotype Columbia-0
SWissProt
Manually annotated by BRENDA team
ATPLC5; ecotype Columbia-0
UniProt
Manually annotated by BRENDA team
ATPLC6; ecotype Columbia-0
UniProt
Manually annotated by BRENDA team
ATPLC7; ecotype Columbia-0
UniProt
Manually annotated by BRENDA team
ATPLC8; ecotype Columbia-0
UniProt
Manually annotated by BRENDA team
ATPLC9; ecotype Columbia-0
UniProt
Manually annotated by BRENDA team
Columbia ecotype
SWissProt
Manually annotated by BRENDA team
four PLC delta isoenzymes: PLC delta1-4
-
-
Manually annotated by BRENDA team
PLC-beta1
-
-
Manually annotated by BRENDA team
serotype C; var. gattii serotype B; var. grubii serotype A
-
-
Manually annotated by BRENDA team
Cryptococcus neoformans H99
serotype C; var. gattii serotype B; var. grubii serotype A
-
-
Manually annotated by BRENDA team
DdPLC, homolog to mammalian PLC-delta
-
-
Manually annotated by BRENDA team
DsPLC2, 6 weeks old
SwissProt
Manually annotated by BRENDA team
NorpA, PLC-21
-
-
Manually annotated by BRENDA team
adult
-
-
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
breast cancer patients
-
-
Manually annotated by BRENDA team
four PLC delta isoenzymes: PLC delta1-4
-
-
Manually annotated by BRENDA team
myelodysplastic syndromes (MDS) patients
UniProt
Manually annotated by BRENDA team
myelodysplastic syndromes (MDS) patients
SwissProt
Manually annotated by BRENDA team
PLC-epsilon
-
-
Manually annotated by BRENDA team
PLCbeta1a and PLCbeta1b
SwissProt
Manually annotated by BRENDA team
PLCbeta1a; two isoforms of PLCbeta1, a and b; PLCbeta1b; two isoforms of PLCbeta1, a and b
SwissProt
Manually annotated by BRENDA team
PLCgamma2
-
-
Manually annotated by BRENDA team
PLCgamma2
SwissProt
Manually annotated by BRENDA team
13 mammalian PLC isozymes have been identified
-
-
Manually annotated by BRENDA team
PLC-beta, -gamma, -delta and -epsilon
-
-
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
-
SWissProt
Manually annotated by BRENDA team
; PLCzeta
SwissProt
Manually annotated by BRENDA team
C57BL strain
-
-
Manually annotated by BRENDA team
C57BL/6
-
-
Manually annotated by BRENDA team
C57BL/6J and CAST/Ei
UniProt
Manually annotated by BRENDA team
PLC-beta
-
-
Manually annotated by BRENDA team
PLCbeta1
-
-
Manually annotated by BRENDA team
PLCbeta1a and PLCbeta1b
SwissProt
Manually annotated by BRENDA team
PLCdelta
-
-
Manually annotated by BRENDA team
PLCdelta1
SwissProt
Manually annotated by BRENDA team
PLCgamma2-deficient mice
UniProt
Manually annotated by BRENDA team
Mus musculus C57BL
C57BL strain
-
-
Manually annotated by BRENDA team
Mus musculus C57BL/6
C57BL/6
-
-
Manually annotated by BRENDA team
Mytilus galloprovincialis Lam
Lam
-
-
Manually annotated by BRENDA team
L. cv. Ningxia
SwissProt
Manually annotated by BRENDA team
PsPLC delta isoform
SwissProt
Manually annotated by BRENDA team
; male Wistar rats
-
-
Manually annotated by BRENDA team
expression in COS-7 cells
-
-
Manually annotated by BRENDA team
female Sprague-Dawley rats
-
-
Manually annotated by BRENDA team
four PLC delta isoenzymes: PLC delta1-4
-
-
Manually annotated by BRENDA team
PH-PLCdelta1 residues 11-140
-
-
Manually annotated by BRENDA team
PLC-delta1
-
-
Manually annotated by BRENDA team
PLCbeta1a and PLCbeta1b
SwissProt
Manually annotated by BRENDA team
PLCepsilon
SwissProt
Manually annotated by BRENDA team
Sprague-Dawley
-
-
Manually annotated by BRENDA team
Sprague-Dawley rats
SwissProt
Manually annotated by BRENDA team
Wistar rats, adult
-
-
Manually annotated by BRENDA team
Rattus norvegicus PLC-delta1
PLC-delta1
-
-
Manually annotated by BRENDA team
Rattus norvegicus PLCepsilon
PLCepsilon
SwissProt
Manually annotated by BRENDA team
Rattus norvegicus Sprague-Dawley
Sprague-Dawley
-
-
Manually annotated by BRENDA team
CEP A stre, YC17, and TS141, the strains are isogenic to W303
UniProt
Manually annotated by BRENDA team
Saccharomyces cerevisiae PLC1p
PLC1p
-
-
Manually annotated by BRENDA team
several strains, 4 genes encoding 1 bacterial type PI-PLC and 3 eukaryotic type PI-PLCs
-
-
Manually annotated by BRENDA team
genes Pi-Plc1 and Pi-Plc2
-
-
Manually annotated by BRENDA team
L. elite inbred line Ye 7922
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
malfunction
-
silencing of PLC4 impairs the race-specific effector Avr4 from the pathogenic fungus Cladosporium fulvum/Cladosporium fulvum-resistant Cf-4 tomato-induced hypersensitive response and results in increased colonisation of Cladosporium fulvum-resistant Cf-4 plants by Cladosporium fulvum expressing Avr4. Silencing of PLC6 does not affect hypersensitive response, whereas it causes increased colonisation of Cf-4 plants by the fungus
malfunction
-
inhibition of PI-PLC in vivo resultes in rapid upregulation of PtdIns(4,5)P2 levels
malfunction
-
isoforms of PI-PLC family display different expression and/or sub cellular distribution under non-physiological conditions such as the rat astrocytes activation during neurodegeneration, the tumoural progression of some neoplasms and the inflammatory cascade activation after lipopolysaccharide administration. PI-PLC beta3 and PI-PLC gamma2 isoforms, whose expression and sub cellular localization significantly differ after U73122 inhibitor treatment
malfunction
-
PLC isozymes are involved in several diseases, detailed overview
physiological function
-
PLC isoforms are required for the hypersensitive response and disease resistance. PLC4 is specifically required for Cladosporium fulvum-resistant Cf-4 tomato function, while PLC6 may be a more general component of resistance protein signalling. PLC4 and PLC6 are involved in Cf-4-mediated resistance to Cladosporium fulvum. PLC6, but not PLC4, is also required for resistance to Verticillium dahliae, mediated by the transmembrane Ve1 resistance protein, and to Pseudomonas syringae, mediated by the intracellular Pto/Prf resistance protein couple of Pseudomonas syringae. Expression of the gene in Nicotiana benthamiana enhances the Avr4/Cf-4-induced hypersensitive response
physiological function
-
Isc1 regulates the level of complex sphingolipids and certain species of phytoceramide, especially when fungal cells are exposed to acidic stress. Either the enzyme Isc1 P-loop is not involved in the substrate recognition or that it is not sufficient to discriminate between phosphoinositide and sphingomyelin
physiological function
Q9NQ66
nuclear PLCbeta1 is a key player in the control of cell cycle progression and has a key role as a check point in the G1 phase of the cell cycle, in fact it appears to be involved in the cyclin-mediated regulation of the physiological machinery
physiological function
Q9Z1B3
nuclear PLCbeta1 is a key player in the control of cell cycle progression and has a key role as a check point in the G1 phase of the cell cycle, in fact it appears to be involved in the cyclin-mediated regulation of the physiological machinery
physiological function
P10687
nuclear PLCbeta1 is a key player in the control of cell cycle progression and has a key role as a check point in the G1 phase of the cell cycle, in fact it appears to be involved in the cyclin-mediated regulation of the physiological machinery
physiological function
-
PI-PLC enzymes interfere with the metabolism of inositol phospholipids, molecules involved in signal transduction, a complex process depending on various components
physiological function
-
PI-PLC shows functional importance in regulating phosphoinositide turnover in Tetrahymena. Eukaryotic PI-PLC specifically hydrolyzes phosphatidylinositol 4,5-bisphosphate to produce the Ca2+-mobilizing agent inositol 1,4,5-trisphosphate, and regulates signaling in multicellular organisms. Bacterial PtdIns-specific PLCs hydrolyze PtdIns and glycosyl-PtdIns, and are considered important virulence factors. Tetrahymena species are unique in that they contain two sets of functional genes coding for both 1 bacterial and 3 eukaryotic PLCs, one of which is inactive. Expression patterns and PI-PLC activities in three Tetrahymena thermophila strains showed a correlation between expression levels and activity, suggesting that the three eukaryotic PI-PLC genes are functionally nonredundant. The two PLC activities differ in their phosphoinositide substrate utilization, subcellular localization, secretion to extracellular space, and sensitivity to Ca2+
physiological function
-
PLC is involved in the regulation of proliferation, differentiation, and tumor formation, the switch between proliferation and differentiation is caused by PLCs. PLC regulates the spatio-temporal balance of phosphoinositides. Calcium mobilization is an essential cellular function of PLC. PLCzeta and PLCdelta4 are involved in the calcium increase in the first step of fertilization. PLCbeta- and PLCeta-type are involved in neuronal function. PLCdelta1 regulates skin homeostasis. The PLCepsilon is an effector of Ras protein, both upstream and downstream, and its activity is regulated by Ras in a GTP-dependent manner. PLC is a soluble protein that is localized mainly in the cytosol and is translocated to the plasma membrane, where it hydrolyzes PI(4,5)P2 in response to cell activation. Thus, targeting of PLC to the plasma membrane is a critical event for signal transduction. Association with heterotrimeric G-proteins of the Gq family induces the activity of beta-type PLCs. gamma-Type isozymes are regulated primarily by receptor and cytosolic tyrosine kinases, mechanisms, overview
physiological function
-
the enzyme is involved in calcium-dependent signalling cascades controlling the life cycle of malaria parasites. Synchronized development of Plasmodium berghei gametocytes relies on rapid calcium release from internal stores within 10 s of gametocytes being exposed to mosquito-derived xanthurenic acid, which triggers the enzyme. Requirement for PI-PLC beyond the early mobilization of calcium, with a complex interdependency of Ca2+ and PI-PLC activity, with PI-PLC being essential throughout gamete formation, possibly explaining the irreversibility of this process
physiological function
Cryptococcus neoformans H99
-
Isc1 regulates the level of complex sphingolipids and certain species of phytoceramide, especially when fungal cells are exposed to acidic stress. Either the enzyme Isc1 P-loop is not involved in the substrate recognition or that it is not sufficient to discriminate between phosphoinositide and sphingomyelin
-
malfunction
Q9NQ66
possible involvement of the interstitial deletion of PLCbeta1 gene in the progression of myelodysplastic syndrome to acute myeloid leukemia in humans
additional information
-
co-expression of PLCeta2 and Gbetagamma protein results in higher PLC activity
additional information
Q9NQ66
method development for preparation of highly purified nuclei and the subsequent analysis of nuclear PLCbeta1 signaling, overview
additional information
Q9Z1B3
method development for preparation of highly purified nuclei and the subsequent analysis of nuclear PLCbeta1 signaling, overview
additional information
P10687
method development for preparation of highly purified nuclei and the subsequent analysis of nuclear PLCbeta1 signaling, overview
additional information
-
protein-protein interactions between the wheat heterotrimeric Ga subunit, GA3, the calcium-binding protein, Clo3 and the phosphoinositide-specific phospholipase C, PI-PLC1, in vivo and in vitro, analysis, overview
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1-acyl-2-arachidonoylphosphatidylinositol + H2O
?
show the reaction diagram
-
-
-
-
?
1-acyl-2-linoleoylphosphatidylinositol + H2O
?
show the reaction diagram
-
-
-
-
?
1-phosphatidyl-1D-myo-inositol
1D-myo-inositol 1-phosphate + diacylglycerol
show the reaction diagram
-
-
in almost equal amounts with 1D-myo-inositol 1,2-cyclic phosphate
-
?
1-phosphatidyl-1D-myo-inositol + H2O
1D-myo-inositol 1-phosphate
show the reaction diagram
-
-
in almost equal amounts with 1D-myo-inositol 1,2-cyclic phosphate
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
-
-
-
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
-
-
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
-
-
-
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
-
-
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
Q8K4D7, Q8R3B1
-
-
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
-
-
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
-
-
-
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
-
-
-
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
-
-
-
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
-
-
-
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
-
-
-
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
-
-
-
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
-
-
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
-
-
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
-
-
-
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
-
-
-
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
-
-
-
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
-
-
-
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
-
-
-
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
Q9NQ66
-
-
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
Q99P84
-
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
Q9FSW1
-
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
P16885
-
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
P16885
-
-
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
O24297
-
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
-
-
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
A2AP18
-
-
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
Q4KWH8
-
-
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
Q7T274
-
-
-
-
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
Q3ZTU7
-
-
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
Q4KWH5
-
-
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
-
-
-
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
Q9Z1B3
-
-
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
-
-
-
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
P10687
-
-
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
Q00722
-
-
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
Q8CIH5
-
-
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
Q6SA76, Q6TM09, Q6TM10
-
-
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
P51178
-
-
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
P19174
-
-
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
-
-
products are two second messengers
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
-
preferred substrate
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
Q39033
AtPLC2, preferred substrate, N-terminal EF-hand-like domain is required for catalytic activity but not for lipid binding
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
-
domain organization of the PLC isoenzymes beta, gamma, delta and epsilon, distinct regulatory domains
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
-
domain structure of PLC-epsilon, contains RasGEF and RA domains
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
Q8K4D7, Q8R3B1
domain structure of PLCdelta1
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
-
domain structure of PLCgamma2, roles of the different domains
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
Q8K4D7, Q8R3B1
domain structure of PLCzeta
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
-
domain structures of PLC-beta, -gamma and -delta isoenzymes
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
-
domain structures of PLCbeta, -gamma and -delta isoenzymes
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
-
phosphoinositide-specific
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
-
phosphoinositide-specific
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
Q9NQ66
phosphoinositide-specific
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
-
phosphoinositide-specific, PLC delta structure with EF-hand motif and PH domain
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
Q9TZN8
phosphoinositide-specific, preferred substrate to phosphatidylinositol, domain structure
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
-
PLC-beta1
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
-
PLC-delta1, structure of the catalytic domain, His-311 and His-356 act as general acid-base catalysts
4- and 5-phosphoryl groups of inositol 1,4,5-trisphosphate interact with the side chains of Lys-32 and Lys-57 and with those of Lys-30, Arg-40 and Lys-57 of PLC-delta1, respectively
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
Q9TZN8
inositol phosphate/diacylglycerol signaling pathway, TcPI-PLC is lipid modified in vivo, TcPI-PLC is developmentally regulated, its expression is induced during the differentiation of trypomastigotes into amastigotes at an early stage in the transformation process
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
-
key enzyme in signal transduction generating the intracellular second messengers DAG and IP3
second messengers
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
Q9NQ66
key step in the intracellular transduction of a large number of extracellular signals, including neurotransmitters and hormones modulating diverse developmental and functional aspects of the central nervous system
second messengers
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
-
part of the phosphoinositide signal transduction pathway
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
Q9FSW1
plays a role in various signal transduction pathways, DsPLC2 plays a role in an early event in the light induction process of C4 photosynthesis
the products act as second messengers in cell signalling
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
-
PLC is crucial for the phosphoinositol pathways and involved in eukaryotic signal transduction as it generates two second messengers, regulation of the PLC delta isoenzymes
second messengers
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
-
PLCbeta1 is involved in nuclear inositol lipid signaling
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
-
regulation of PLC isoenzymes, cellular signaling
intracellular messengers
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
Q8K4D7, Q8R3B1
sperm-specific PLCzeta induces intracellular Ca2+ oscillations and subsequent early embryonic development when expressed in mouse eggs, PLCzeta may be the physiological mammalian egg-activating sperm factor
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
-
amino acids Pro500 and His503 are critical for binding of PLC-gamma1 to one of its substrates, 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate in the membrane
-
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
-
PLC beta1b, PLC-delta1, PLC-gamma1
-
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
Q9NQ66
PI-PLC beta1 could have a role in muscle hypertrophy taking place in human muscular dystrophy
-
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
-
PI-PLC beta1 is essential in the IGF-1 mitogenic signaling pathway
-
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
-
saPLC1 employs a unique catalytic mechanism whereby the cleavage of phosphatidylinositol into inositol 1-phosphate occurs via a trans cyclization mechanism involving formation of inositol 1,6-cyclic phosphate as an intermediate
-
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
-
interaction analysis, modeling and binding kinetics of substrate and enzyme PH-PLCdelta1, overview. PH-PLCdelta1 binds specifically to 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate, breaking up clusters and expelling nonspecifically associated peptide from the surface
-
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
Saccharomyces cerevisiae PLC1p
-
preferred substrate
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
Rattus norvegicus PLCepsilon
Q99P84
-
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
Rattus norvegicus PLC-delta1
-
PLC-delta1, structure of the catalytic domain, His-311 and His-356 act as general acid-base catalysts
4- and 5-phosphoryl groups of inositol 1,4,5-trisphosphate interact with the side chains of Lys-32 and Lys-57 and with those of Lys-30, Arg-40 and Lys-57 of PLC-delta1, respectively
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
Mytilus galloprovincialis Lam
-
-
-
-
?
arachidonoyl-phosphatidylinositol + H2O
1,2-diarachidonoyl-sn-glycerol + 1D-myo-inositol 1-phosphate
show the reaction diagram
-
-
-
-
?
arachidonoylphosphatidylinositol + H2O
myo-inositol 1-phosphate + 1,2-diarachidonoylglycerol
show the reaction diagram
-
-
-
?
diarachidonoylphosphatidylinositol + H2O
1,2-diacylglycerol + 1D-myo-inositol 1-phosphate
show the reaction diagram
-
-
a mixture of 86% myoinositol 1-phosphate and 14% myoinositol 1,2-cyclic phosphate
-
?
dibutyrylphosphatidylinositol + H2O
?
show the reaction diagram
P34024
-
-
-
?
glycosylphosphatidylinositol + H2O
?
show the reaction diagram
-
catalytic mechanism
-
?
phosphatidylinositol + H2O
?
show the reaction diagram
-
-
-
-
?
phosphatidylinositol + H2O
?
show the reaction diagram
-
-
-
-
?
phosphatidylinositol + H2O
?
show the reaction diagram
-
-
-
-
?
phosphatidylinositol + H2O
?
show the reaction diagram
-
-
-
?
phosphatidylinositol + H2O
?
show the reaction diagram
-
-
-
-
?
phosphatidylinositol + H2O
?
show the reaction diagram
-
-
-
-
?
phosphatidylinositol + H2O
?
show the reaction diagram
Q3ZTU7
-
-
-
?
phosphatidylinositol + H2O
?
show the reaction diagram
-
no activity
-
-
-
phosphatidylinositol + H2O
?
show the reaction diagram
-
phosphatidylinositol 4,5-bisphosphate is a better substrate
-
-
?
phosphatidylinositol + H2O
?
show the reaction diagram
-
at neutral pH and low Ca2+ concentration the membrane bound enzyme hydrolyzes phosphatidylinositol 4,5-diphosphate in preference to phosphatidylinositol
-
-
?
phosphatidylinositol + H2O
?
show the reaction diagram
-
7% of the activity with phosphatidylinositol 4,5-diphosphate
-
-
?
phosphatidylinositol + H2O
?
show the reaction diagram
-
Ca2+ required. At 0.1 mM the enzyme hydrolyzes phosphatidylinositol 4,5-diphosphate at faster rate than phosphatidylinositol, at 0.005 mM Ca2+ it hydrolyzes phosphatidylinositol 4,5-diphosphate exclusively
-
-
?
phosphatidylinositol + H2O
?
show the reaction diagram
-
phosphatidylinositol monolayer at an air/water interface
-
-
?
phosphatidylinositol + H2O
?
show the reaction diagram
-
PLC-delta1, structure of the catalytic domain
-
?
phosphatidylinositol + H2O
?
show the reaction diagram
-
low molecular weight PLC hydrolyzes both phosphatidylinositol and phosphatidylinositol 4,5-bisphosphate, high molecular PLC shows much greater activity against phosphatidylinositol than phosphatidylinositol 4,5-bisphosphate
-
-
?
phosphatidylinositol + H2O
?
show the reaction diagram
Rattus norvegicus Sprague-Dawley, Mus musculus C57BL
-
-
-
-
?
phosphatidylinositol + H2O
?
show the reaction diagram
Rattus norvegicus PLC-delta1
-
PLC-delta1, structure of the catalytic domain
-
?
phosphatidylinositol + H2O
myo-inositol 1-phosphate + diacylglycerol
show the reaction diagram
-
-
-
?
phosphatidylinositol + H2O
myo-inositol 1-phosphate + diacylglycerol
show the reaction diagram
Q39033
60-350times lower specific activity than with phosphatidylinositol 4,5-bisphosphate
-
?
phosphatidylinositol + H2O
myo-inositol 1-phosphate + diacylglycerol
show the reaction diagram
Q9TZN8
phosphatidylinositol 4,5-bisphosphate is preferred to phosphatidylinositol
-
?
phosphatidylinositol + H2O
diacylglycerol + myo-inositol 1-phosphate
show the reaction diagram
-
phosphatidylinositol-specific, catalytic mechanism
-
?
phosphatidylinositol + H2O
diacylglycerol + inositol 1,2-(cyclic)-phosphate
show the reaction diagram
P34024
-
-
-
?
phosphatidylinositol + H2O
1,2-diacylglycerol + 1D-myo-inositol 1-phosphate
show the reaction diagram
-
-
1D-myo-inositol 1,2-cyclic phosphate is also formed
-
?
phosphatidylinositol + H2O
1D-myo-inositol 1-phosphate
show the reaction diagram
-
-
in vitro also inositol cyclic monophosphate formed
-
?
phosphatidylinositol + H2O
1D-myo-inositol 1-phosphate
show the reaction diagram
-
-
in vitro myo-inositol 1,2-cyclic phosphate also formed
-
?
phosphatidylinositol + H2O
1D-myo-inositol 1-phosphate
show the reaction diagram
-
enzyme hydrolyses both membrane-bound and extracted phosphatidylinositol
in vitro myo-inositol 1,2-cyclic phosphate, myo-inositol 1-phosphate, and glycerophosphoinositol in the molar proportions approx. 2:1:1
-
?
phosphatidylinositol + H2O
1D-myo-inositol 1-phosphate + diacylglycerol
show the reaction diagram
-
-
-
-
?
phosphatidylinositol + H2O
1D-myo-inositol 1-phosphate + diacylglycerol
show the reaction diagram
-
-
1D-myo-inositol 1,2-cyclic-phosphate is also formed
-
?
phosphatidylinositol + H2O
1D-myo-inositol 1-phosphate + diacylglycerol
show the reaction diagram
-
-
in vitro also 1D-myo-inositol 1,2-cyclic phosphate formed
-
?
phosphatidylinositol + H2O
1D-myo-inositol 1-phosphate + diacylglycerol
show the reaction diagram
-
enzyme is phosphatidylinositol specific
-
-
?
phosphatidylinositol + H2O
1D-myo-inositol 1-phosphate + glycerophosphoinositol + phosphate
show the reaction diagram
-
-
ratio between products decreased with increasing pH, 1D-myo-inositol 1,2-cyclic phosphate is also formed
-
?
phosphatidylinositol + H2O
1D-myo-inositol-1-phosphate
show the reaction diagram
-
-
in vitro 1D-myo-inositol-1,2-cyclic phosphate is also formed
-
?
phosphatidylinositol + H2O
diacylglycerol + 1D-myo-inositol 1-phosphate
show the reaction diagram
-
-
the lipoidal product of hydrolysis is diacylglycerol, the water-soluble products are 1D-myo-inositol 1,2-cyclic phosphate and 1D-myo-inositol 1-phosphate in the approximate proportions of 60% and 40%, respectively
-
?
phosphatidylinositol + H2O
diacylglycerol + 1D-myo-inositol 1-phosphate
show the reaction diagram
-
enzyme is specific for phosphatidylinositol
-
-
?
phosphatidylinositol + H2O
diacylglycerol + 1D-myo-inositol 1-phosphate
show the reaction diagram
-
Pi-PLC (membrane) and Pi-PLC (cytosol) are specific for this substrate
-
-
?
phosphatidylinositol 4,5-bisphosphate
inositol 1,4,5-trisphosphate
show the reaction diagram
-
demonstration of the role of PLC in Ca2+-induced inactivation of TRPV6
-
-
?
phosphatidylinositol 4,5-bisphosphate + H2O
D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
-
-
-
?
phosphatidylinositol 4,5-bisphosphate + H2O
D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
-
-
-
-
?
phosphatidylinositol 4,5-bisphosphate + H2O
D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
-
-
-
?
phosphatidylinositol 4,5-bisphosphate + H2O
D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
-
-
-
-
?
phosphatidylinositol 4,5-bisphosphate + H2O
D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
-
-
-
-
?
phosphatidylinositol 4,5-bisphosphate + H2O
D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
-
-
-
-
?
phosphatidylinositol 4,5-bisphosphate + H2O
D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
-
-
-
-
?
phosphatidylinositol 4,5-bisphosphate + H2O
D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
-
-
-
-
?
phosphatidylinositol 4,5-bisphosphate + H2O
D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
-
-
-
-
?
phosphatidylinositol 4,5-bisphosphate + H2O
D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
-
-
-
-
?
phosphatidylinositol 4,5-bisphosphate + H2O
D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
-
-
-
?
phosphatidylinositol 4,5-bisphosphate + H2O
D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
B2LSE7
-
-
-
?
phosphatidylinositol 4,5-bisphosphate + H2O
D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
-
-
Ca2+ required. At 0.1 mM the enzyme hydrolyzes phosphatidylinositol 4,5-diphosphate at faster rate than phosphatidylinositol, at 0.005 mM Ca2+ it hydrolyzes phosphatidylinositol 4,5-diphosphate exclusively
?
phosphatidylinositol 4,5-bisphosphate + H2O
D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
-
-
isoenzyme PLC beta1 produces 21% inositol 1:2,4,5 cyclic trisphosphate at pH 5.5 and 7.5% at pH 7.0
?
phosphatidylinositol 4,5-bisphosphate + H2O
D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
-
preferentially hydrolyzed
-
-
?
phosphatidylinositol 4,5-bisphosphate + H2O
D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
-
membrane-bound enzyme hydrolyzes phosphatidylinositol 4,5-diphosphate in preference to phosphatidylinositol
-
?
phosphatidylinositol 4,5-bisphosphate + H2O
D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
-
enzyme attacks phosphatidylinositol 4,5-diphosphate and phosphatidylinositol 4-phosphate with equal facility
-
-
?
phosphatidylinositol 4,5-bisphosphate + H2O
D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
-
the enzyme generates two second messengers: D-myo-inositol 1,4,5-trisphosphate and diacylglycerol
-
?
phosphatidylinositol 4,5-bisphosphate + H2O
?
show the reaction diagram
-
-
-
-
?
phosphatidylinositol 4,5-bisphosphate + H2O
?
show the reaction diagram
-
enzyme activities for phosphatidylinositol 4,5-bisphosphate are strongly dependent on the relative levels of calcium and pH in the assay buffer
-
-
?
phosphatidylinositol 4,5-bisphosphate + H2O
diacylglycerol + 1D-myo-inositol 1,4,5-triphosphate
show the reaction diagram
-
PLC4 and PLC6 encode catalytically active enzymes that convert phosphatidylinositol into diacylglycerol
-
-
?
phosphatidylinositol 4-phosphate
?
show the reaction diagram
-
-
-
-
?
phosphatidylinositol 4-phosphate
?
show the reaction diagram
-
-
-
-
?
phosphatidylinositol 4-phosphate
?
show the reaction diagram
-
enzyme attacks phosphatidylinositol 4,5-diphosphate and phosphatidylinositol 4-phosphate with equal facility
-
-
?
phosphatidylinositol 4-phosphate
?
show the reaction diagram
-
phosphatidylinositol 4,5-bisphosphate is a better substrate
-
-
?
phosphatidylinositol 4-phosphate
?
show the reaction diagram
-
20% of the activity with phosphatidylinositol 4,5-diphosphate
-
-
?
phosphatidylinositol 4-phosphate + H2O
1D-myo-inositol 4,5-bisphosphate + diacylglycerol
show the reaction diagram
-
-
-
-
?
phosphatidylinositol 4-phosphate + H2O
1D-myo-inositol 4,5-bisphosphate + diacylglycerol
show the reaction diagram
-
-
-
?
phosphatidylinositol 4-phosphate + H2O
?
show the reaction diagram
-
-
-
-
?
phosphatidylinositol 4-phosphate + H2O
?
show the reaction diagram
Rattus norvegicus, Rattus norvegicus PLC-delta1
-
PLC-delta1, structure of the catalytic domain
-
?
GPI-renal dipeptidase
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
not: phosphatidyl choline, phosphatidyl ethanolamine, phosphatidyl serine
-
?
additional information
?
-
-
PI-PLC has a high affinity for the glycosylphosphatidylinositol anchor of GPI-anchored proteins, e.g. 5-nucleotidase and alkaline phosphatase, and catalyzes cleavage in vitro to release a soluble protein and diacylglycerol, which remains in the membrane
-
?
additional information
?
-
-
PI-PLC has a high affinity for the glycosylphosphatidylinositol anchor of GPI-anchored proteins, e.g. 5-nucleotidase and alkaline phosphatase, and catalyzes cleavage in vitro to release a soluble protein and diacylglycerol, which remains in the membrane, enzyme structure
-
?
additional information
?
-
Q99P84
PLCepsilon contains a GTP exchange factor and two C-terminal Ras-binding domains
-
?
additional information
?
-
-
the activation of the gamma1 isoenzyme is a biologically important event in zona pellucida-stimulated inositol phospholipid metabolism with a potential role in signal transduction leading to acrosomal exocytosis
-
-
-
additional information
?
-
-
regulation of purified subtypes of phosphatidylinositol-specific phospholipase C beta by G protein alpha and betagamma subunits is dependent of enzyme subtype and concentration of Mg2+
-
-
-
additional information
?
-
-
the inositol lipid breakdown is controlled by different phosphoinositidase C isoenzymes in various cell compartments
-
-
-
additional information
?
-
-
the activation of the enzyme by m3-mascarinic acid receptor stimulation is mediated via alpha-subunit of the Gq/11 family of G-proteins
-
-
-
additional information
?
-
-
perturbation in a membrane in vivo equivalent to a non-bilayer configuration is sufficient to induce phosphodiesterase-catalyzed phosphatidylinositol 4,5-diphosphate breakdown
-
-
-
additional information
?
-
-
the enzyme may participate in signal transduction over the plant plasma membrane
-
-
-
additional information
?
-
-
plays a critical role in signal transduction in the receptor-activated human platelets
-
-
-
additional information
?
-
-
transformation-linked and progression-linked signal transduction enzyme
-
-
-
additional information
?
-
-
hepatocyte growth factor can activate phosphoinositidase C in rat hepatocytes
-
-
-
additional information
?
-
-
AtPLC1S is involved in stress responses, AtPLC2 not
-
?
additional information
?
-
Q39033
AtPLC2 is constitutively expressed
-
?
additional information
?
-
-
cellular signaling
-
?
additional information
?
-
-
cellular signaling, regulation of PLC activity
-
?
additional information
?
-
-
PLC delta1 is required for skin stem cell lineage commitment
-
?
additional information
?
-
-
PLC plays a key role in photoreceptor response
-
?
additional information
?
-
-
PLC-beta1 is nessecary for the development and/or maintenance of brain inhibitory pathways, the retinal variants of PLC-beta4 function in signal processing within the retina, PLC-beta3 plays a role in the early development
-
?
additional information
?
-
Q99P84
PLCepsilon is a Ras effector, directly regulated by Ras and links two critical signaling pathways
-
?
additional information
?
-
P16885
PLCgamma isoforms are regulated through activation of tyrosine kinase-linked receptors, importance of PLCgamma2 in B-cell signal transduction
-
?
additional information
?
-
-
PLCgamma2 regulation, role in B-cell signalling
-
?
additional information
?
-
O24297
PsPLC delta isoform is regulated by light in a tissue specific manner and may be involved in important functions
-
?
additional information
?
-
-
activation of phospholipase C is a vital step in the neuronal protective adenosine-induced signal transduction pathway, conferring protection against iodoacetic acid-induced injury in primary rat neuronal cultures
-
-
-
additional information
?
-
-
Galpha12712- and Rho-dependent activation of phospholipase C-epsilon by lysophosphatidic acid and thrombin receptors
-
-
-
additional information
?
-
-
Gi-coupled receptors can inhibit PLC-epsilon, most likely by suppressing formation of cyclic AMP required for Epac-mediated Rap2B activation
-
-
-
additional information
?
-
-
interaction of phospholipase C-beta1 with cell polarity Par proteins may serve as a nexus to transduce extracellular signals to transcriptional reguation through G-protein-mediated signaling pathway in cell polarity and cell asymmetric division
-
-
-
additional information
?
-
-
involvement of phospholipase C signaling in melanoma cell-induced endothelial junction disassembly
-
-
-
additional information
?
-
-
phospholipase C epsilon modulates beta-adrenergic receptor-dependent cardiac contraction and inhibits cardiac hypertrophy
-
-
-
additional information
?
-
-
phospholipase C is a key factor determining the photoreceptor sensitivity
-
-
-
additional information
?
-
Q2YGU0, Q2YGU1
PI-PLC activity might be regulated by a heterotrimeric G protein
-
-
-
additional information
?
-
-
PIP2-PLC is involved in the signal pathway that leads to the acquisition of heat acclimation
-
-
-
additional information
?
-
-
PLC activity is under tight dynamic control of the Ca2+ concentration. In insulin-secreting beta-cells, this mechanism provides a link between Ca2+ influx and release from intracellular stores that may be important in the regulation of insulin secretion
-
-
-
additional information
?
-
-
PLC is involved in activation of the muscarinic receptor-operated cationic current in guinea pig ileal smooth muscle cells
-
-
-
additional information
?
-
-
PLC-eta enzymes may function in the membrane-mediated signaling cascade through intracellular Ca2+ and protein kinase C pathways
-
-
-
additional information
?
-
Q4KWH8
PLC-eta may perform fundamental roles in the brain
-
-
-
additional information
?
-
Q4KWH5
PLC-eta may perform fundamental roles in the brain
-
-
-
additional information
?
-
-
PLCgamma2 is crucial for development of the NL cell receptor repertoire and signaling of activating NK cell receptors, mediating optimal NL cell function in vivo
-
-
-
additional information
?
-
Q8K4D7, Q8R3B1
recombinant PLCzeta protein induces Ca2+ oscillations in mouse eggs
-
-
-
additional information
?
-
-
reduction in phospholipase C may be critical in the pathophysiology of depression and other stress-related disorders
-
-
-
additional information
?
-
Q9P212
regulatory mechanisms controlling expression of PLCepsilon, broadened by diversity introduced by splice variants, could play important role in PLCepsilon regulation in normal and tumor cells
-
-
-
additional information
?
-
-
signaling of myelin phagocytosis mediated by complement-receptor-3 alone and by complement-receptor-3 combined with scavenger-receptor-AI/II involves phosphatidylinositol 3-kinase, phosphoinositide-specific phospholipase-Cgamma and protein kinase-C, the cascade phosphatidylinositol 3-kinase -> phosphoinositide-specific phospholipase-Cgamma -> protein kinase-C, and wide range modulation by protein kinase-C
-
-
-
additional information
?
-
Q766D4
significant role for PpPLC1 in cytokinin signalling and gravitropism
-
-
-
additional information
?
-
-
suPLCbeta is activated by G protein betagamma subunits
-
-
-
additional information
?
-
Q766D4
the enzyme is involved in cytokinin and gravity responses
-
-
-
additional information
?
-
A2AP18
the neuron-specific enzyme may be important for the formation and/or maintenance of the neuronal network in the postnatal brain
-
-
-
additional information
?
-
Q6SA76, Q6TM09, Q6TM10
the Vr-PLC3 protein is specifically activated by drough and salt stress in an abscisic acid-independent manner, with its induction being faster in roots than in leaf tissue
-
-
-
additional information
?
-
-
when expressed in plasma membrane, TcPI-PLC is involved in differentiation of trypomastigotes to amastigotes of Trypanosoma cruzi, an essential step for the intracellular replication of these parasites
-
-
-
additional information
?
-
-
wild-type Rac GTPase with guanosine 5'-(3-O-thio)triphosphate causes a marked stimulation of PLCgamma2 but has no effect on the activity of PLCgamma1. The mechanism of PLCgamma2 activation by Rac GTPase involves neither protein phosphorylation nor PI3K-mediated generation of phosphatidylinositol 3,4,5-trisphosphate
-
-
-
additional information
?
-
Q62077
absolute requirement for PLC-gamma1 in villin-induced cell migration
-
-
-
additional information
?
-
-
activation of PLCgamma by GTP during initial stages of formation of nuclear envelope provides a mechanism for temporal control of assembly of nuclear envelope
-
-
-
additional information
?
-
-
inhibition of phosphatidylinositol 3-kinase may go through phosphoinositide-specific phospholipase C pathway leading to increase of expression of the inducible nitric oxide synthase, indicating that phosphatidylinositol 3-kinase and phosphoinositide-specific phospholipase C may counteract to regulate the metabolism of phosphatidylinositol (4,5)-bisphosphate depending on cell types or their conditions
-
-
-
additional information
?
-
-
inositide-dependent phospholipase C signaling mimics insulin in skeletal muscle differentiation by affecting specific regions of the cyclin D3 promoter
-
-
-
additional information
?
-
Q9Z1B3
overview: role of nuclear PI-PLCbeta1 in cell proliferation and differentiation
-
-
-
additional information
?
-
P10687
overview: role of nuclear PI-PLCbeta1 in cell proliferation and differentiation
-
-
-
additional information
?
-
-
phosphatidylinositol-phospholipase C plays a critical role, most likely through activation of protein kinase C pathway, in TLR4 mediated immune responses of avian macrophage cells to lipopolysaccharides
-
-
-
additional information
?
-
Q01970
phospholipase C beta3 is a key component in the Gbetagamma/PKCeta/PKD-mediated regulation of trans-Golgi network to plasma membrane transport
-
-
-
additional information
?
-
-
PLC eta enzyme influences intracellular Ca 2+ dynamics and protein kinase C activation in the brain and neuroendocrine systems as putative mediation of G-protein-coupled receptor regulation likely mediates G-protein-coupled receptor signalling pathways
-
-
-
additional information
?
-
-
PLC eta enzyme influences intracellular Ca 2+ dynamics and protein kinase C activation in the brain and neuroendocrine systems as putative mediation of G-protein-coupled receptor regulationlikely mediates G-protein-coupled receptor signalling pathways
-
-
-
additional information
?
-
-
PLC-beta2 may improve some malignant characteristics of tumor cells, like motility and invasion capability, but it fails to induce tumorigenesis in nontransformed breast-derived cells
-
-
-
additional information
?
-
-
PLC-dsu is unlikely to be directly involved in the process of egg activation but may play a role in mediating extracellular signals transmitted via the phosphatidylinositol 3'-kinase pathway
-
-
-
additional information
?
-
Q8K4S1
PLCepsilon is required for thrombin- but not lysophosphatidic acid-induced sustained ERK activation and DNA synthesis, providing a novel mechanism for G protein-coupled receptor and Rho signaling to cell proliferation. PLCepsilon has a role as a guanine nucleotide exchange factor for Rap1
-
-
-
additional information
?
-
-
suppression of Pi-PLC promotes apoptosis of vascular endothelial cells by inhibiting phosphorylation of Akt, elevating P53 expression, and affecting the cell cycle distribution. phosphatidyl-specific phospholipase C and phosphatidylcholine-specific phospholipase C might control the apoptosis by jointly regulating akt phosphorylation, P53 expression and affecting cell cycle in vascular endothelial cells
-
-
-
additional information
?
-
Q8CIH5
PLCgamma2 is essential for RANK (Receptor activator of NF-kB) signaling, and its deficiency leads to defective lymph node organogenesis and osteoclast differentiation
-
-
-
additional information
?
-
-
the enzymes acting against [3H] inositol-phosphatidylinositol (PtdIns) and [3H] inositolphosphatidylinositol-4,5-bisphosphate [PtdIns(4,5)P2] in the cat carotid bodies (CB) are investigated using labelled phospholipids as a source of the substrate. Analysis of water soluble inositol metabolites indicates that PtdIns is degraded exclusively by the enzyme since the inositolmonophosphate (IP1) is detected as a single product. In case of PtdIns(4,5)P2 hydrolysis by carotid bodies (CB) enzymes there are all 3 inositol phosphates: IP3, IP2, IP1
-
-
-
additional information
?
-
P34024
water-soluble synthetic short-chain phosphatidylinositol and inositol 1,2-(cyclic)-phosphate along with PI/detergent mixed micelles and PI/PC vesicles are used as substrates
-
-
-
additional information
?
-
-
enzyme is phosphatidylinositol-specific and does not act on phosphatidylethanolamine and phosphatidylcholine
-
-
-
additional information
?
-
-
enzyme is specific for phosphoinositides
-
-
-
additional information
?
-
-
PLC activity in vascular smooth muscle cells can be divided into two types based on their calcium and pH requirements, substrate preferences, and molecular weights, several different assay conditions for both phosphatidylinositol and phosphatidylinositol 4,5-bisphosphate are tested on samples of crude supernatant for their ability to support PLC activity, the injection fraction shows no activity against phosphatidylcholine
-
-
-
additional information
?
-
-
enzyme is not active with phosphatidylcholine and phosphatidylethanolamine as substrate
-
-
-
additional information
?
-
-
no activity is detectable towards either phosphatidylcholine or phosphatidylethanolamine
-
-
-
additional information
?
-
-
no detectable activity against phosphatidylcholine or phosphatidylethanolamine
-
-
-
additional information
?
-
-
no phospholipase A2 activity is found in the purified enzymes
-
-
-
additional information
?
-
-
phosphatidylcholine, phosphatidylethanolamine, or phosphatidylserine are no substrates
-
-
-
additional information
?
-
-
Isc1 only exerts IPC-PLC activity
-
-
-
additional information
?
-
-
PLCepsilon exhibits activity as RasGEF toward Rap1, but not to Ras, Rap2A or Rho
-
-
-
additional information
?
-
-
synthesis of a short-chain (dihexanoyl) analogues of phosphatidylinositol, which exist as monomers at the concentrations used for the kinetic measurements, the cleavage proceeds with the release of the cyclic phosphorothioate
-
-
-
additional information
?
-
Rattus norvegicus PLCepsilon
Q99P84
PLCepsilon contains a GTP exchange factor and two C-terminal Ras-binding domains, PLCepsilon is a Ras effector, directly regulated by Ras and links two critical signaling pathways
-
?
additional information
?
-
Cryptococcus neoformans H99
-
Isc1 only exerts IPC-PLC activity
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
-
-
-
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
Q9NQ66
-
-
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
-
-
products are two second messengers
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
Q9TZN8
inositol phosphate/diacylglycerol signaling pathway, TcPI-PLC is lipid modified in vivo, TcPI-PLC is developmentally regulated, its expression is induced during the differentiation of trypomastigotes into amastigotes at an early stage in the transformation process
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
-
key enzyme in signal transduction generating the intracellular second messengers DAG and IP3
second messengers
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
Q9NQ66
key step in the intracellular transduction of a large number of extracellular signals, including neurotransmitters and hormones modulating diverse developmental and functional aspects of the central nervous system
second messengers
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
-
part of the phosphoinositide signal transduction pathway
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
Q9FSW1
plays a role in various signal transduction pathways, DsPLC2 plays a role in an early event in the light induction process of C4 photosynthesis
the products act as second messengers in cell signalling
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
-
PLC is crucial for the phosphoinositol pathways and involved in eukaryotic signal transduction as it generates two second messengers, regulation of the PLC delta isoenzymes
second messengers
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
-
PLCbeta1 is involved in nuclear inositol lipid signaling
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
-
regulation of PLC isoenzymes, cellular signaling
intracellular messengers
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
Q8K4D7, Q8R3B1
sperm-specific PLCzeta induces intracellular Ca2+ oscillations and subsequent early embryonic development when expressed in mouse eggs, PLCzeta may be the physiological mammalian egg-activating sperm factor
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
Q9NQ66
PI-PLC beta1 could have a role in muscle hypertrophy taking place in human muscular dystrophy
-
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
-
PI-PLC beta1 is essential in the IGF-1 mitogenic signaling pathway
-
-
?
phosphatidylinositol 4,5-bisphosphate + H2O
D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
-
the enzyme generates two second messengers: D-myo-inositol 1,4,5-trisphosphate and diacylglycerol
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
-
saPLC1 employs a unique catalytic mechanism whereby the cleavage of phosphatidylinositol into inositol 1-phosphate occurs via a trans cyclization mechanism involving formation of inositol 1,6-cyclic phosphate as an intermediate
-
-
?
additional information
?
-
-
the activation of the gamma1 isoenzyme is a biologically important event in zona pellucida-stimulated inositol phospholipid metabolism with a potential role in signal transduction leading to acrosomal exocytosis
-
-
-
additional information
?
-
-
regulation of purified subtypes of phosphatidylinositol-specific phospholipase C beta by G protein alpha and betagamma subunits is dependent of enzyme subtype and concentration of Mg2+
-
-
-
additional information
?
-
-
the inositol lipid breakdown is controlled by different phosphoinositidase C isoenzymes in various cell compartments
-
-
-
additional information
?
-
-
the activation of the enzyme by m3-mascarinic acid receptor stimulation is mediated via alpha-subunit of the Gq/11 family of G-proteins
-
-
-
additional information
?
-
-
perturbation in a membrane in vivo equivalent to a non-bilayer configuration is sufficient to induce phosphodiesterase-catalyzed phosphatidylinositol 4,5-diphosphate breakdown
-
-
-
additional information
?
-
-
the enzyme may participate in signal transduction over the plant plasma membrane
-
-
-
additional information
?
-
-
plays a critical role in signal transduction in the receptor-activated human platelets
-
-
-
additional information
?
-
-
transformation-linked and progression-linked signal transduction enzyme
-
-
-
additional information
?
-
-
hepatocyte growth factor can activate phosphoinositidase C in rat hepatocytes
-
-
-
additional information
?
-
-
AtPLC1S is involved in stress responses, AtPLC2 not
-
?
additional information
?
-
Q39033
AtPLC2 is constitutively expressed
-
?
additional information
?
-
-
cellular signaling
-
?
additional information
?
-
-
cellular signaling, regulation of PLC activity
-
?
additional information
?
-
-
PLC delta1 is required for skin stem cell lineage commitment
-
?
additional information
?
-
-
PLC plays a key role in photoreceptor response
-
?
additional information
?
-
-
PLC-beta1 is nessecary for the development and/or maintenance of brain inhibitory pathways, the retinal variants of PLC-beta4 function in signal processing within the retina, PLC-beta3 plays a role in the early development
-
?
additional information
?
-
Q99P84
PLCepsilon is a Ras effector, directly regulated by Ras and links two critical signaling pathways
-
?
additional information
?
-
P16885
PLCgamma isoforms are regulated through activation of tyrosine kinase-linked receptors, importance of PLCgamma2 in B-cell signal transduction
-
?
additional information
?
-
-
PLCgamma2 regulation, role in B-cell signalling
-
?
additional information
?
-
O24297
PsPLC delta isoform is regulated by light in a tissue specific manner and may be involved in important functions
-
?
additional information
?
-
-
activation of phospholipase C is a vital step in the neuronal protective adenosine-induced signal transduction pathway, conferring protection against iodoacetic acid-induced injury in primary rat neuronal cultures
-
-
-
additional information
?
-
-
Galpha12712- and Rho-dependent activation of phospholipase C-epsilon by lysophosphatidic acid and thrombin receptors
-
-
-
additional information
?
-
-
Gi-coupled receptors can inhibit PLC-epsilon, most likely by suppressing formation of cyclic AMP required for Epac-mediated Rap2B activation
-
-
-
additional information
?
-
-
interaction of phospholipase C-beta1 with cell polarity Par proteins may serve as a nexus to transduce extracellular signals to transcriptional reguation through G-protein-mediated signaling pathway in cell polarity and cell asymmetric division
-
-
-
additional information
?
-
-
involvement of phospholipase C signaling in melanoma cell-induced endothelial junction disassembly
-
-
-
additional information
?
-
-
phospholipase C epsilon modulates beta-adrenergic receptor-dependent cardiac contraction and inhibits cardiac hypertrophy
-
-
-
additional information
?
-
-
phospholipase C is a key factor determining the photoreceptor sensitivity
-
-
-
additional information
?
-
Q2YGU0, Q2YGU1
PI-PLC activity might be regulated by a heterotrimeric G protein
-
-
-
additional information
?
-
-
PIP2-PLC is involved in the signal pathway that leads to the acquisition of heat acclimation
-
-
-
additional information
?
-
-
PLC activity is under tight dynamic control of the Ca2+ concentration. In insulin-secreting beta-cells, this mechanism provides a link between Ca2+ influx and release from intracellular stores that may be important in the regulation of insulin secretion
-
-
-
additional information
?
-
-
PLC is involved in activation of the muscarinic receptor-operated cationic current in guinea pig ileal smooth muscle cells
-
-
-
additional information
?
-
-
PLC-eta enzymes may function in the membrane-mediated signaling cascade through intracellular Ca2+ and protein kinase C pathways
-
-
-
additional information
?
-
Q4KWH8
PLC-eta may perform fundamental roles in the brain
-
-
-
additional information
?
-
Q4KWH5
PLC-eta may perform fundamental roles in the brain
-
-
-
additional information
?
-
-
PLCgamma2 is crucial for development of the NL cell receptor repertoire and signaling of activating NK cell receptors, mediating optimal NL cell function in vivo
-
-
-
additional information
?
-
Q8K4D7, Q8R3B1
recombinant PLCzeta protein induces Ca2+ oscillations in mouse eggs
-
-
-
additional information
?
-
-
reduction in phospholipase C may be critical in the pathophysiology of depression and other stress-related disorders
-
-
-
additional information
?
-
Q9P212
regulatory mechanisms controlling expression of PLCepsilon, broadened by diversity introduced by splice variants, could play important role in PLCepsilon regulation in normal and tumor cells
-
-
-
additional information
?
-
-
signaling of myelin phagocytosis mediated by complement-receptor-3 alone and by complement-receptor-3 combined with scavenger-receptor-AI/II involves phosphatidylinositol 3-kinase, phosphoinositide-specific phospholipase-Cgamma and protein kinase-C, the cascade phosphatidylinositol 3-kinase -> phosphoinositide-specific phospholipase-Cgamma -> protein kinase-C, and wide range modulation by protein kinase-C
-
-
-
additional information
?
-
Q766D4
significant role for PpPLC1 in cytokinin signalling and gravitropism
-
-
-
additional information
?
-
-
suPLCbeta is activated by G protein betagamma subunits
-
-
-
additional information
?
-
Q766D4
the enzyme is involved in cytokinin and gravity responses
-
-
-
additional information
?
-
A2AP18
the neuron-specific enzyme may be important for the formation and/or maintenance of the neuronal network in the postnatal brain
-
-
-
additional information
?
-
Q6SA76, Q6TM09, Q6TM10
the Vr-PLC3 protein is specifically activated by drough and salt stress in an abscisic acid-independent manner, with its induction being faster in roots than in leaf tissue
-
-
-
additional information
?
-
-
when expressed in plasma membrane, TcPI-PLC is involved in differentiation of trypomastigotes to amastigotes of Trypanosoma cruzi, an essential step for the intracellular replication of these parasites
-
-
-
additional information
?
-
-
wild-type Rac GTPase with guanosine 5'-(3-O-thio)triphosphate causes a marked stimulation of PLCgamma2 but has no effect on the activity of PLCgamma1. The mechanism of PLCgamma2 activation by Rac GTPase involves neither protein phosphorylation nor PI3K-mediated generation of phosphatidylinositol 3,4,5-trisphosphate
-
-
-
additional information
?
-
Q62077
absolute requirement for PLC-gamma1 in villin-induced cell migration
-
-
-
additional information
?
-
-
activation of PLCgamma by GTP during initial stages of formation of nuclear envelope provides a mechanism for temporal control of assembly of nuclear envelope
-
-
-
additional information
?
-
-
inhibition of phosphatidylinositol 3-kinase may go through phosphoinositide-specific phospholipase C pathway leading to increase of expression of the inducible nitric oxide synthase, indicating that phosphatidylinositol 3-kinase and phosphoinositide-specific phospholipase C may counteract to regulate the metabolism of phosphatidylinositol (4,5)-bisphosphate depending on cell types or their conditions
-
-
-
additional information
?
-
-
inositide-dependent phospholipase C signaling mimics insulin in skeletal muscle differentiation by affecting specific regions of the cyclin D3 promoter
-
-
-
additional information
?
-
Q9Z1B3
overview: role of nuclear PI-PLCbeta1 in cell proliferation and differentiation
-
-
-
additional information
?
-
P10687
overview: role of nuclear PI-PLCbeta1 in cell proliferation and differentiation
-
-
-
additional information
?
-
-
phosphatidylinositol-phospholipase C plays a critical role, most likely through activation of protein kinase C pathway, in TLR4 mediated immune responses of avian macrophage cells to lipopolysaccharides
-
-
-
additional information
?
-
Q01970
phospholipase C beta3 is a key component in the Gbetagamma/PKCeta/PKD-mediated regulation of trans-Golgi network to plasma membrane transport
-
-
-
additional information
?
-
-
PLC eta enzyme influences intracellular Ca 2+ dynamics and protein kinase C activation in the brain and neuroendocrine systems as putative mediation of G-protein-coupled receptor regulation likely mediates G-protein-coupled receptor signalling pathways
-
-
-
additional information
?
-
-
PLC eta enzyme influences intracellular Ca 2+ dynamics and protein kinase C activation in the brain and neuroendocrine systems as putative mediation of G-protein-coupled receptor regulationlikely mediates G-protein-coupled receptor signalling pathways
-
-
-
additional information
?
-
-
PLC-beta2 may improve some malignant characteristics of tumor cells, like motility and invasion capability, but it fails to induce tumorigenesis in nontransformed breast-derived cells
-
-
-
additional information
?
-
-
PLC-dsu is unlikely to be directly involved in the process of egg activation but may play a role in mediating extracellular signals transmitted via the phosphatidylinositol 3'-kinase pathway
-
-
-
additional information
?
-
Q8K4S1
PLCepsilon is required for thrombin- but not lysophosphatidic acid-induced sustained ERK activation and DNA synthesis, providing a novel mechanism for G protein-coupled receptor and Rho signaling to cell proliferation. PLCepsilon has a role as a guanine nucleotide exchange factor for Rap1
-
-
-
additional information
?
-
-
suppression of Pi-PLC promotes apoptosis of vascular endothelial cells by inhibiting phosphorylation of Akt, elevating P53 expression, and affecting the cell cycle distribution. phosphatidyl-specific phospholipase C and phosphatidylcholine-specific phospholipase C might control the apoptosis by jointly regulating akt phosphorylation, P53 expression and affecting cell cycle in vascular endothelial cells
-
-
-
additional information
?
-
Q8CIH5
PLCgamma2 is essential for RANK (Receptor activator of NF-kB) signaling, and its deficiency leads to defective lymph node organogenesis and osteoclast differentiation
-
-
-
additional information
?
-
-
the enzymes acting against [3H] inositol-phosphatidylinositol (PtdIns) and [3H] inositolphosphatidylinositol-4,5-bisphosphate [PtdIns(4,5)P2] in the cat carotid bodies (CB) are investigated using labelled phospholipids as a source of the substrate. Analysis of water soluble inositol metabolites indicates that PtdIns is degraded exclusively by the enzyme since the inositolmonophosphate (IP1) is detected as a single product. In case of PtdIns(4,5)P2 hydrolysis by carotid bodies (CB) enzymes there are all 3 inositol phosphates: IP3, IP2, IP1
-
-
-
additional information
?
-
-
enzyme is phosphatidylinositol-specific and does not act on phosphatidylethanolamine and phosphatidylcholine
-
-
-
additional information
?
-
-
enzyme is specific for phosphoinositides
-
-
-
additional information
?
-
-
PLC activity in vascular smooth muscle cells can be divided into two types based on their calcium and pH requirements, substrate preferences, and molecular weights, several different assay conditions for both phosphatidylinositol and phosphatidylinositol 4,5-bisphosphate are tested on samples of crude supernatant for their ability to support PLC activity, the injection fraction shows no activity against phosphatidylcholine
-
-
-
additional information
?
-
-
Isc1 only exerts IPC-PLC activity
-
-
-
additional information
?
-
-
PLCepsilon exhibits activity as RasGEF toward Rap1, but not to Ras, Rap2A or Rho
-
-
-
additional information
?
-
Rattus norvegicus PLCepsilon
Q99P84
PLCepsilon is a Ras effector, directly regulated by Ras and links two critical signaling pathways
-
?
additional information
?
-
Cryptococcus neoformans H99
-
Isc1 only exerts IPC-PLC activity
-
-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
(NH4)2SO4
-
high salt concentration, (NH4)2SO4, 2 M, dissociates the high molecular weight form yielding the low molecular form and increasing the specific activity
Ca2+
-
at 0.01 mM 20% stimulation, at 0.1 mM 32% stimulation
Ca2+
-
activates, maximal activity at 0.01 mM
Ca2+
-
required, maximal hydrolysis of phosphatidylinositol 4,5-diphosphate at 0.004 mM, maximal hydrolysis of phosphatidylinositol at 0.5 mM
Ca2+
-
stimulates, maximal activity at 0.1 mM
Ca2+
-
dependent on, maximal activity at 100 nM
Ca2+
-
required
Ca2+
-
activates
Ca2+
-
maximally active with Ca2+ concentrations ranging from endogenous levels to 1 mM for the substrates phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol 4-phosphate
Ca2+
-
absolute requirement for both isoenzymes. Maximal activity with phosphatidylinositol at pH 5.5: 1 mM. Maximal activity with phosphatidylinositol 4,5-diphosphate at pH 7.0: 0.1 mM
Ca2+
-
maximal activity at 1 mM
Ca2+
-
PLC requires calcium for full activation
Ca2+
-
significant hydrolysis of phosphatidylinositol only above 0.1 mM
Ca2+
-
Ca2+ required. At 0.1 mM the enzyme hydrolyzes phosphatidylinositol 4,5-diphosphate at faster rate than phosphatidylinositol, at 0.005 mM Ca2+ it hydrolyzes phosphatidylinositol 4,5-diphosphate exclusively
Ca2+
-
dependend
Ca2+
-
enzyme requires 2-5 mM CaCI2 for maximal activity
Ca2+
-
optimal concentration: 2.0-3.0 mM: the activity at pH 5.5 unlike that at pH 7.4 is not absolutely dependent on Ca2+
Ca2+
-
stimulates the activity and can not be replaced by MnCl2, MgCl2 or ZnCl2
Ca2+
-
stimulates
Ca2+
-
dependend on
Ca2+
-
dependent on
Ca2+
-
dependent on
Ca2+
-
dependent on, concentration of 5 mM CaCl2 produces optimum deacylation of phosphatidylinositol
Ca2+
-
required
Ca2+
-
enzyme has an absolute requirement for Ca2+
Ca2+
-
optimal concentrations are 0.3 mM and 8 mM using 0.2 mM and 0.4 mM phosphatidylinositol
Ca2+
-
enzyme requires a bivalent cation, Ca2+ being the most effective activator
Ca2+
-
activated by, optimal concentration 1 mM
Ca2+
-
PIPLC (cytosol) presents a total dependence on the presence of Ca2+ in the assay, which cannot be substituted by other divalent cations (Mg2+, Mn2+, Zn2+)
Ca2+
-
requirement, PLC isoenzymes beta, gamma, delta and epsilon, delta-type isoenzymes are most sensitive
Ca2+
-
requirement, mode of Ca2+ binding at the catalytic domain of PLC-delta1
Ca2+
-
requirement, phosphatidic acid reduces the Ca2+ concentration required for half maximal stimulation of PLC-beta1 activity
Ca2+
Q99P84
Ca2+-dependent, maximum activity at 800 nM free Ca2+
Ca2+
Q39033
AtPLC2 requires low micromolar levels of Ca2+, maximal phosphatidylinositol 4,5-bisphosphate hydrolysis at 0.001 mM free Ca2+, hydrolysis of phosphatidylinositol requires higher Ca2+ concentrations
Ca2+
Q9TZN8
dependent on free Ca2+, enzyme needs a higher calcium concentration for its activation with phosphatidylinositol as substrate than with phosphatidylinositol 4,5-bisphosphate
Ca2+
P16885
PLCgamma2, Ca2+-dependent, highest activity at 0.005-0.01 mM
Ca2+
Q8K4D7, Q8R3B1
activates PLCdelta1, EC50: 0.0057 mM, PLCdelta1 has a 100fold lower Ca2+ sensitivity than PLCzeta; activity of PLC-delta1 increases at a Ca2+ concentration between 0.001 and 0.03 mM; PLCzeta, 70% of maximal activity at 100 nM Ca2+, EC50: 52 nM, PLCzeta has a 100fold higher Ca2+ sensitivity than PLCdelta1; PLCzeta exhibites a significant activity at Ca2+ concentration of 10 nM and has 70% maximal activity at 100 nM Ca2+ that is the basal intracellular calcium ion concentration level of cells
Ca2+
Q9FSW1
activates, the two recombinant enzymes synthesized from two potential initiation codons differ in their Ca2+ requirements
Ca2+
-
activates, maximal activity at 0.01 mM
Ca2+
O24297
coordinated at the active site
Ca2+
-
maximal activation of PI-PLC by 2 mM CaCl2, Ca2+-dependent phosphoinositide degradation
Ca2+
-
Ca2+-dependent
Ca2+
Q4KWH8
recombinant enzyme is Ca2+-dependent
Ca2+
Q4KWH5
recombinant enzyme is Ca2+-dependent
Ca2+
Q3ZTU7
when phosphatidylinositol is used as a substrate, the enzyme activity increases with increasing free Ca2+ concentrations above 0.001 mM, and the activity decreases when free Ca2+-concentration exceeded 1 mM. When 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate is used as substrate, the enzyme is activated at lower Ca2+-concentrations and its activity increases with increasing free Ca2+ concentration. Addition of 3 mM MgCl2 stimulates hydrolysis of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate and inhibites hydrolysis of phosphatidylinositol
Ca2+
Q7T274
dependent on
Ca2+
A2AP18
maximal activity at 0.001 mM
Ca2+
-
-
Ca2+
-
an equilibrium between nuclear import and export is shifted by intracellular Ca2+ levels, since PLCdelta1 forms a complex with importin beta1 in the presence of physiological concentrations of Ca2+. the Ca2+-sensing domain is the catalytic core of PLCdelta1
Ca2+
-
-
Ca2+
-
induction of cell Ca2+ disorders can play a primary role in the occurrence of heavy metal cytotoxic effects
Ca2+
-
PLC/IP3-mediated Ca2+ release after muscarinic receptor stimulation does only account for less than 15% of the overall induced Ca2+ signal and Ca2+-dependent contraction in murine urinary bladder smooth muscle
Ca2+
-
-
Ca2+
-
Ca2+ flowing activates phospholipase C leading to the depletion of phosphatidylinositol 4,5-bisphosphate and formation of inositol 1,4,5-trisphosphate in TRPV6-expressing cells
Ca2+
-
unphysiologically high Ca2+ levels can overcome PI-PLC inhibition by U73122
Ca2+
-
required, one of the isozymes is activated by low-micromolar Ca2+
Ca2+
-
required
Ca2+
Q9Z1B3
required
Ca2+
-
all PLC isozymes require calcium for their activity, the PLCdelta-type is one of the most sensitive to calcium, suggesting that the activity of the PLCdelta-type may be regulated by calcium. PLCzeta is extremely sensitive to the calcium concentration
CaCl2
-
stimulates at pH 5.5, pH dependent
K+
-
stimulates
K+
-
stimulates at pH 5.5
Mg2+
-
can partially replace Ca2+ in activation, 15% of the activity with Ca2+ at 0.01 mM
Mg2+
-
activates
Mg2+
-
stimulates
Mg2+
-
calcium, manganese, magnesium and zinc ions, in decreasing order of effectiveness, all partly restore the activity of the dialysed enzyme at a concentration of 2 mM. In each case 4 mM cation is less effective than 2 mM
Mg2+
-
activated to a lesser extent than Ca2+ by
Mg2+
-
no requirement for the membrane enzyme solubilized with sodium cholate, activates the membrane enzyme after solubilization in octyl glucoside with maximal activity at 0.1 mM
Mg2+
Q3ZTU7
slight stimulation of hydrolysis of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
MgCl2
-
stimulates at pH 5.5, but less active than CaCl2, pH dependent
Mn2+
-
stimulates
NaCl
-
marked activation in presence of lecithin or phosphatidylethanolamine
NaCl
-
inhibits phosphatidylinositol hydrolysis of low molecular weight PLC. Conversely, PIP2 hydrolysis is stimulated by increasing concentrations of NaCl
Zn2+
-
calcium, manganese, magnesium and zinc ions, in decreasing order of effectiveness, all partly restore the activity of the dialysed enzyme at a concentration of 2 mM. In each case 4 mM cation is less effective than 2 mM
Mn2+
-
calcium, manganese, magnesium and zinc ions, in decreasing order of effectiveness, all partly restore the activity of the dialysed enzyme at a concentration of 2 mM. In each case 4 mM cation is less effective than 2 mM
additional information
-
no absolute requirement
additional information
-
Ba2+, Zn2+, Hg2+, K+ are ineffective; certain divalent cations are necessary for enzyme activity
additional information
-
bivalent ions (Ca2+, Mg2+, Co2+, Ni2+ and Mn2+) facilitate enzyme penetration into substrate monolayers
additional information
-
non-physiologically low level of Ca2+ (below 100 nM) suppresses both basal and light-induced phospholipase C activity
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
(1 -[6-[[17-beta-3-methoxyestra-1,3,5(10)-trien-17-yl]amino] hexyl]-1H-pyrrole-2,5-dione)
-
-
1,2-bis(2-aminophenoxy)ethane N,N,N',N'-tetraacetic acid
-
-
1-(6-((17beta-3-methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl)-1H-pyrrole-2,5-dione
-
significant inhibition of PLC-beta with IC50: 0.006 mM, little effect on PLC-beta1, PLC-beta3, or PLC-beta4
1-(6-((17beta-3-methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl)-1H-pyrrole-2,5-dione
-
U-73122
1-(6-((17beta-3-methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl)-1H-pyrrole-2,5-dione
-
U73122, also inhibits Ca2+-induced inactivation of TRPV6
1-O-octadecyl-2-O-methyl-rac-glycero-3-phosphocholine
-
potent inhibitor
1-O-octadecyl-2-O-methyl-sn-glycero-3-phosphorylcholine
-
histamine-induced elevation of plasma noradrenaline and adrenaline is dose-dependently reduced by ET-18-OCH3
3beta,27-dihydroxyurs-12-en-28-oic acid
-
-
3beta,7'-diacetoxy-27- 4-(E)-coumaroyloxyurs-12-en-28-oic acid
-
-
3beta-4-(E)-coumaroyloxyurs-12-en-28-oic acid
-
-
3beta-hydroxy-27-(E)-feruloyloxyolea-12-en-28-oic acid
-
-
3beta-hydroxy-27-(E)-feruloyloxyurs-12-en-28-oic acid
-
-
3beta-hydroxy-27-(Z)-feruloyloxyolea-12-en-28-oic acid
-
-
3beta-hydroxy-27-(Z)-feruloyloxyurs-12-en-28-oic acid
-
-
3beta-hydroxy-27-4-(E)-2'-dihydrocoumaroyloxyurs-12-en-28-oic acid
-
-
3beta-hydroxy-27-4-(E)-coumaroyloxyolea-12-en-28-oic acid
-
-
3beta-hydroxy-27-4-(E)-coumaroyloxyurs-12-en-28-oic acid
-
-
3beta-hydroxy-27-4-(E)-coumaroyloxyurs-12-en-28-oic methyl ester
-
-
3beta-hydroxy-27-4-(Z)-coumaroyloxyolea-12-en-28-oic acid
-
-
3beta-hydroxy-27-4-(Z)-coumaroyloxyurs-12-en-28-oic acid
-
-
3beta-hydroxyurs-12-en-28-oic 4-(E)-coumaroyl ester
-
-
4-(E)-coumaric acid
-
-
48/80
-
compound 48/80, oligomeric mixture of condensation products of N-methyl-p-methoxyphenethylamine and formaldehyde
aggregated amyloid beta protein
-
full-length Abeta 1-40, aggregated form inhibits Ca2+-regulated phosphoinositide degradation by cytosolic and membrane-bound PLC by 30-40%, inhibitor may interact with the Ca2+-binding site of enzyme, no inhibition by nonaggregated soluble full-length amyloid beta protein
-
amentoflavone
-
-
anionic amphipathic substances
-
-
-
ATP
-
1-5 mM
Ba2+
-
at 0.05-5 mM, inhibition is completely reversed by Ca2+
Blood lipoprotein
-
enzyme is strongly inhibited by blood plasma, serum and blood lipoproteins
-
Blood plasma
-
enzyme is strongly inhibited by blood plasma, serum and blood lipoproteins
-
Blood serum
-
enzyme is strongly inhibited by blood plasma, serum and blood lipoproteins
-
Ca2+
-
above 0.01 mM
Ca2+
-
above 0.1 mM
Ca2+
-
at high concentrations
Ca2+
-
concentrations above 1 mM are inhibitory
Ca2+
Q3ZTU7
when phosphatidylinositol is used as a substrate, the enzyme activity increases with increasing free Ca2+ concentrations above 1 M, and the activity decreases when free Ca2+-concentration exceeded 1 mM. When 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate is used as substrate, the enzyme is activated at lower Ca2+-concentrations and its activity increases with increasing free Ca2+ concentration. Addition of 3 mM MgCl2 stimulates hydrolysis 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate and inhibites hydrolysis of phosphatidylinositol
CaCl2
-
above 0.4 mM, pH 7.0
camphor
-
inhibit the phospholipase C signaling
chlorpromazine
-
at pH 7.0
cholic acid
-
inhibits PLC-beta1 activity
choline plasmalogen
-
18.5% of choline plasmalogen is needed for 50% inhibition
Cinchocaine
-
at pH 7.0
cinnamaldehyde
-
inhibit the phospholipase C signaling
CRM-51006
-
i.e. 1-(3-ethyl-2,4-dihydroxyphenyl)-2-methylhexan-1-one
Cu2+
-
is effective only after preincubation, suggesting that the effect of the metal may be derived from lipid peroxidation due to Cu2+-induced oxyradical production
CuCl2
-
0.1 mM, 53% inhibition
Cys
-
5 mM, 58% inhibition
deoxycholate
-
weak inhibition. Concentration of 2 mM inhibits the phospholipase C activity in all fractions by 20-30%
deoxycholate
-
0.1 mM, inhibits basal and phosphatidic acid-stimulated PLC-beta1 activity
dibromo 1,2-bis(2-aminophenoxy)ethane N,N,N',N'-tetraacetic acid
-
-
Diethyl ether
-
25% v/v, 22% inhibition
edelfosin
-
-
edelfosine
-
ET-18-OCH3, also inhibits Ca2+-induced inactivation of TRPV6
EDTA
-
1 mM, 52% inhibition
EDTA
-
complete inhibition at 1 mM
EDTA
-
-
EDTA
-
at 0.05-5 mM, inhibition is completely reversed by Ca2+
EDTA
-
2 mM, 70% inhibition
EDTA
-
addition of EDTA completely inhibits the activity
EDTA
-
inhibition by EDTA can be reverted upon the addition of increasing concentrations of Ca2+ and Mg2+ in the assay
EGTA
-
-
EGTA
-
at 0.05-5 mM, inhibition is completely reversed by Ca2+
guanosine 5'-[gamma-thio]triphosphate
-
activity is downregulated by 1-10 mM guanosine 5'-[gamma-thio]triphosphate
Hg2+
-
0.1 mM, 90% inhibition
Hg2+
-
inhibits PLC activity when added directly to the reaction mixture. Toxic effect stronger than that of Cu2+ both in vitro and in vivo, especially for the digestive gland
iodoacetamide
-
1.0 mM, 16% inhibition
La3+
-
at 0.05-5 mM, inhibition is completely reversed by Ca2+
lysocholine plasmalogen
-
8.5% of choline plasmalogen is needed for 50% inhibition
-
lysophosphatidylcholine
-
50% inhibition is produced by 7% molar
lysophosphatidylcholine
-
C12, C14 and C16 lysophosphatidylcholines give progressive inhibition with increasing chain length
menthol
-
inhibit the phospholipase C signaling
Mg2+
-
at 0.05-5 mM, inhibition is completely reversed by Ca2+
Mg2+
Q3ZTU7
hydrolysis of phosphatidylinositol
MgCl2
-
0.1-1 mM, 20% inhibition
MgCl2
-
1 mM MgCl2, in presence of free Ca2+
MgCl2
-
above 0.4 mM, pH 7.0
NaCl
-
inhibits phosphatidylinositol hydrolysis of low molecular weight PLC. Conversely, PIP2 hydrolysis is stimulated by increasing concentrations of NaCl
NEM
-
5 mM, 34% inhibition
neomycin
-
-
neomycin
Q3ZTU7
hydrolysis of phosphatidylinositol and 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
nod factor
-
activity is decreased by up to 64% in the presence of increasing concentrations of Nod factors
-
Octadecylamine
-
decreases the film pressure of the phosphatidylinositol monolayer at an air/water interface at which cut-off occurs, as well as the rate of hydrolysis below this pressures
oleoyl sphingomyelin
-
-
Palmitoylcholine
-
decreases the film pressure of the phosphatidylinositol monolayer at an air/water interface at which cut-off occurs, as well as the rate of hydrolysis below this pressures
PCMB
-
0.5 mM, 78% inhibition
Phenylmercuriacetate
-
-
Phenylmercuriacetate
-
0.1 mM, 85% inhibition
phosphatidylcholine
-
low effect, 50% inhibition is produced by 18% molar egg phosphatidylcholine
phosphatidylcholine
-
little effect, 50% inhibition is produced by 18% molar egg phosphatidylcholine
phosphatidylcholine
-
decreases the film pressure of the phosphatidylinositol monolayer at an air/water interface at which cut-off occurs, as well as the rate of hydrolysis below this pressures
phosphatidylcholine
-
the enzyme is strongly inhibited when ovophosphatidylcholine and saturated phosphatidylcholines with acyl chain length of more than eight carbon atoms are mixed with its substrate
phosphatidylcholine
-
slightly inhibited, 30% at concentrations equimolar with phosphatidylinositol
phosphatidylethanolamine
-
25% inhibition, in presence of 0.001 mM Ca2+
phosphatidylserine
-
50% inhibition, in presence of 0.001 mM Ca2+
phosphatidylserine
-
30 mol%, slight PLC-beta1 inhibition
reduced glutathione
-
-
reduced glutathione
-
-
reduced glutathione
-
6.8 mM, 35% inhibition
RhoA
P51178
-
Sodium deoxycholate
-
activates from 0.05% to 0.08%, but severely inhibits activity above 0.08%, no activity at 0.3%
sphingomyelin
-
50% inhibition at 6.5% molar, and 97% at 20% molar
sphingomyelin
-
50% inhibition at low concentration, and 97% at high concentration
sphingosylphosphocholine
-
-
Sr2+
-
at higher concentrations (greater than 0.25 mM) markedly inhibited, inhibition is completely reversed by Ca2+
stearoyl sphingomyelin
-
-
substance 48/80
Q3ZTU7
inhibition is more pronounced with phosphatidylinositol as substrate than with 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
Triton X-100
-
inhibits PLC-beta1 activity
U-73122
-
-
U-73122
Q9FSW1
aminosteroid inhibitor the two recombinant enzymes synthesized from two potential initiation codons differ in the sensitivity
U-73122
-
histamine-induced elevation of plasma noradrenaline and adrenaline is dose-dependently reduced by U-73122
U-73122
-
specific PLC inhibitor
U-73122
-
-
U-73122
B4XX94, B4XX95
PLC inhibitor, inhibits growth of Cryptococcus neoformans; PLC inhibitor, inhibits growth of Cryptococcus neoformans
U-73122
-
PLC inhibitor inhibits Ca2+-induced inactivation of TRPV6 in patch-clamp experiments
U-73343
-
inactive analog of U-73122
U73122
-
effectively blocks the effect of insulin on the release of GPI-renal dipeptidase
U73122
-
direct activation of PI-PLC though Ca2+ may bypass inhibition by U73122, PI-PLC activity is not sensitive to U73122 when activated by the Ca2+ ionophore A23187. Unphysiologically high Ca2+ levels can overcome PI-PLC inhibition by U73122
U73122
-
i.e. 1-(6-([17beta-3-methoxyestra-1,3,5(10)-trien-17-yl]amino)hexyl)-1H-pyrrole-2,5-dione, one of the isozymes is inhibited
Mn2+
-
at 0.05-5 mM, inhibition is completely reversed by Ca2+
additional information
-
enzyme is not inhibited by 1.7 mM p-bromophenacylbromide
-
additional information
-
enzyme is substantially inhibited by all phospholipids containing a phosphocholine head group. The sphingosine-containing phospholipids are the most inhibitory; phosphatidic acid does not inhibit
-
additional information
-
dialysis of the Apium graveolens var. dulce extract against water completely inhibits the activity
-
additional information
-
regulation of the PLC delta isoenzymes
-
additional information
-
regulation of the PLC delta isoenzymes, under hyperoxia conditions acrylonitrile decreases PLC delta1 level, PLC delta4 gene is down-regulated after spinal cord injury
-
additional information
Q9FSW1
not inhibited by U-73343
-
additional information
P16885
LY294002 inhibits the BCR-induced PLC activation in Ramos cells in a dose-dependent manner
-
additional information
-
D609 has no effect
-
additional information
-
mepacrine (PLA2 inhibitor) and D609 (phosphatidylcholine-specific PLC inhibitor) have no effect. Histamine-induced elevation of plasma noradrenaline and adrenaline is also dose-dependently reduced by RHC-80267 (diacylglycerol lipase inhibitor)
-
additional information
-
experimental metastasis assays with tetracycline in nude mice show that inducible knockdown of PLCgamma1 strongly inhibits development of MDA-MB-231-derived lung metastasis and reverts metastasis formation
-
additional information
-
treatment of mussels with sublethal concentrations of Hg2+ or Cu2+ in vivo produces significant PLC inhibition after 1 or 4 days, respectively. A recovery is reached after 7 days of in vivo metal incubation. Data indicate that in mussel gills and digestive gland heavy metals impair PLC activity, thereby affecting IP3-dependent Ca2+ signaling
-
additional information
-
SFD-1-mediated inactivation of ERM (ezrin/radixin/moesin) proteins is blocked by phospholipase C inhibitors
-
additional information
-
increases in PLC beta1, beta3, gamma1, and delta1 activities and gene expression in response to norepinephrine are prevented by prazosin, an alpha1-adrenoceptor antagonist; increases in PLC beta1, beta3, gamma1, and delta1 activities and gene expression in response to norepinephrine are prevented by prazosin, an alpha1-adrenoceptor antagonist; increases in PLC beta1, beta3, gamma1, and delta1 activities and gene expression in response to norepinephrine are prevented by prazosin, an alpha1-adrenoceptor antagonist; increases in PLC beta1, beta3, gamma1, and delta1 activities and gene expression in response to norepinephrine are prevented by prazosin, an alpha1-adrenoceptor antagonist
-
additional information
B4XX94, B4XX95
inactive analogue control (U73343) has no antifungal activity against Cryptococcus neoformans; inactive analogue control (U-73343) has no antifungal activity against Cryptococcus neoformans. Deltaplc1 is hypersusceptible to flucytosine, azoles and amphotericin B, but not to caspofungin
-
additional information
-
inactive analog U73343 also used
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
(2-{4-methoxy-3,5-bis-[2-methoxy-5-(2-methylamino-ethyl)-benzyl]-phenyl}-ethyl)-methyl amine
-
compound 48/80 i.e. (2-{4-methoxy-3,5-bis-[2-methoxy-5-(2-methylamino-ethyl)-benzyl]-phenyl}-ethyl)-methyl amine, CAS REG. NO.: 94724-12-6, the calmodulin antagonists activate at least one form of the rod outer segment enzyme by a calmodulin-independent mechanism
2-nonenal
-
at 1-100 nM: activates PL-C in DMSO-differentiated HL-60 cells, at 1-10 nM: activates PL-C in undifferentiated HL-60 cells
4-hydroxynonenal
-
at 10-1000 nM, activates PL-C in both undifferentiated and DMSO-differentiated HL-60 cells
activated Galpha proteins
-
-
-
Albumin
-
maximal activation of hydrolysis of phosphatidylinositol 4,5-diphosphate at 0.0125 mg/ml
-
arachidonic acid
-
enhances hyrolysis of phosphatidylinositol 4,5-diphosphate, membrane bound enzyme and cytosolic enzyme
ARGHAP6
P51178
a GTPase-activating protein for RhoA. Activates PLC-gamma1 and increases the Vmax of PLC-beta1 and enhances its response to Ca2+ stimulation. ARHGAP6 protein binds to and up-regulates PLC-beta1 both under in vitro and in vivo conditions
-
B cell antigen receptor
-
induces activation of PLC-gamma2, pathway
-
beta-estradiol
-
-
Ca2+
-
requirement of all PLCs, the delta isoenzymes are most sensitive to Ca2+
Ca2+
-
requirement
Ca2+
-
requirement of all PLCs, the delta isoenzymes are most sensitive to Ca2+
Ca2+
-
the enzyme acting on PtdIns is Ca2+-dependent, in contrary to that acting on PtdIns(4,5)P2 which remains active in the presence of 10 mM EGTA
Calmidazolium
-
the calmodulin antagonists activate at least one form of the rod outer segment enzyme by a calmodulin-independent mechanism
Calmodulin antagonists
-
activate at least one form of the rod outer segment enzyme by a calmodulin-independent mechanism
-
Carbachol
-
small stimulatory effect of about 30% on the enzyme acting on PtdIns(4,5)P2. GTPgammaS enhances this effect
Cetyltrimethylammonium bromide
-
stimulates
chlorpromazine
-
stimulates at pH 5.5
Cinchocaine
-
stimulates at pH 5.5
cytochrome c
-
maximal activation of hydrolysis of phosphatidylinositol 4,5-diphosphate at 0.0125 mg/ml
decanoylglycerophosphate
-
only little activating effect
decanoyllysophosphatidylcholine
-
C10 lysophosphatidylcholine activates the reaction
deoxycholate
-
stimulates activity of enzyme I, II and III at pH 7
deoxycholate
-
required for activity with membrane-bound phosphatidylinositol, slight stimulation with isolated phosphatidylinositol
deoxycholate
-
-
diacylglycerol
-
enhances hyrolysis of phosphatidylinositol 4,5-diphosphate, membrane bound enzyme and cytosolic enzyme
diacylglycerol
-
the addition of choline phospholipids containing two long hydrophobic chains or choline lysophospholipids containing one long hydrophobic chain produced a total inhibition of the activation which had been produced by diacylglycerol
diacylglycerol
-
5-6fold stimulation of rat brain phosphatidylinositol phosphodiesterase. The addition of choline phospholipids containing two long hydrophobic chains or choline lysophospholipids containing one long hydrophobic chain produced a total inhibition of the activation which had been produced by diacylglycerol
diacylglycerol
-
little activating effect on PLC-beta1
Diethyl ether
-
stimulates
dihexanoylglycerophosphocholine
-
-
dopamine
-
small stimulatory effect of about 30% on the enzyme acting on PtdIns(4,5)P2. GTPgammaS enhances this effect
Epidermal growth factor
Q99P84
stimulates PLCepsilon overexpressed in COS-7 cells
Epidermal growth factor
Q9P212
epidermal growth factor stimulates PI-PLC activity of both splice variants
G protein alpha12
-
activates PLC-epsilon
-
G protein betagamma
-
activates PLC-beta isoenzymes with the exception of PLC-beta4
-
G protein betagamma
-
may be the main positive regulator of DdPLC
-
G protein qalpha
-
activates PLC-beta isoenzymes, G protein-coupled receptor-mediated activation
-
Gbetagamma
-
G protein betagamma subunits, the reconstituted enzymes, like wild-type PLC-beta2, are activated by Gbetagamma
-
GTPgammaS
-
PtdIns(4,5)P2-PLC is stimulated by GTPgammaS. Enzyme acting on PtdIns is not activated by GTPgS
GTPgammaS
-
one of the isozymes is activated by GTPgammaS in vitro
histone
-
activates
histone
-
maximal activation of hydrolysis of phosphatidylinositol 4,5-diphosphate at 0.0125 mg/ml
inositol 1,2-(cyclic)-phosphate
P34024
-
Insulin
-
in part, may activate GPI-PLC, via phosphatidylinositol-specific phospholipase C/intracellular Ca2+, which may consequently stimulate the release of GPI-renal dipeptidase
-
insulin-like growth factor 1
-
stimulates nuclear PLCbeta1, through translocation of MAP kinase to the nucleus
-
lysophosphatidic acid
-
slight activation, less effective in stimulating PLC-beta1 activity than phosphatidic acid
mannitol
Q6SA76, Q6TM09, Q6TM10
drought treatment; drought treatment; drought treatment
MAP kinase
-
phosphorylates and activates nuclear PLCbeta1
-
Mastoparan
-
activity almost doubled in the presence of 5 microM
N-(4-aminobutyl)-5-chloro-1-naphthalenesulfonamide
-
the calmodulin antagonists activate at least one form of the rod outer segment enzyme by a calmodulin-independent mechanism
N-(6-Aminohexyl)-5-chloro-1-naphthalenesulfonamide
-
the calmodulin antagonists activate at least one form of the rod outer segment enzyme by a calmodulin-independent mechanism
NaCl
Q6SA76, Q6TM09, Q6TM10
salt treatment; salt treatment; salt treatment
oleic acid
-
causes a marked stimulation of the enzyme activity when the membrane-bound substrate is used
oleic acid
-
markedly increases rate of hydrolysis
oleic acid
-
little activating effect on PLC-beta1
oleoylglycerophosphate
-
activates the hydrolysis at a physiological pH
oleyl alcohol
-
increases the film pressure of the phosphatidylinositol monolayer at an air/water interface at which cut-off occurs, as well as increases the rate of hydrolysis at lower pressures
palmitoylglycerophosphate
-
activates the hydrolysis at a physiological pH
phosphatidic acid
-
enhances hyrolysis of phosphatidylinositol 4,5-diphosphate, membrane bound enzyme and cytosolic enzyme
phosphatidic acid
-
increases the film pressure of the phosphatidylinositol monolayer at an air/water interface at which cut-off occurs, as well as increases the rate of hydrolysis at lower pressure
phosphatidic acid
-
activates the hydrolysis at a physiological pH
phosphatidic acid
-
stimulating
phosphatidic acid
-
PLC-beta1 activation, Ca2+-dependent, relatively independent of acyl chain length, stimulates basal and receptor-G protein-regulated PLC-beta1 activity, activation occurs through an C-terminal-dependent and an independent mechanism
phosphatidylcholine
-
enhances hyrolysis of phosphatidylinositol 4,5-diphosphate, membrane bound enzyme
phosphatidylethanolamine
-
enhances hyrolysis of phosphatidylinositol 4,5-diphosphate, membrane bound enzyme and cytosolic enzyme
phosphatidylethanolamine
-
stimulation in presence of 1 mM Ca2+
phosphatidylethanolamine
-
activates the hydrolysis. Human platelet phosphatidylinositol phosphodiesterase
phosphatidylethanolamine
-
slightly increases rate of hydrolysis
phosphatidylglycerol
-
increases the film pressure of the phosphatidylinositol monolayer at an air/water interface at which cut-off occurs, as well as increases the rate of hydrolysis at lower pressures
phosphatidylserine
-
enhances hyrolysis of phosphatidylinositol 4,5-diphosphate, membrane bound enzyme
phosphatidylserine
-
stimulation in presence of 1 mM Ca2+
protamine
-
activates
PTEN
-
ubiquitously expressed tumor suppressor. PTEN overexpression results in changes in cellular phospholipid levels. Increased PTEN expression in unstimulated MCF-7 breast cancer cells results in a 51% increase in phosphatidic acid, with a decrease in phosphatidylcholine, PTEN activates also phospholipase D (PLD). PTEN induces translocation of PLC-gamma from the cytosol to the membrane
-
Q61L H-Ras
Q99P84
constitutively active Q61L H-Ras stimulates PLCepsilon co-expressed in COS-7 cells in parallel with Ras binding
-
Ras
-
activator of PLC-epsilon
-
receptor protein tyrosine kinase
-
activates PLC-gamma, growth factor receptor-mediated activation
-
RhoA
-
PLC-epsilon is a direct downstream efector for RhoA. RhA-dependent activation of PLC-epsilon depends on a unique insert within the catalytic core of the phospholipase
Sodium deoxycholate
-
activates from 0.05% to 0.08%, but severely inhibits activity above 0.08%, no activity at 0.3%
soluble amyloid beta protein fragment 25-35
-
activates PI-PLC
-
spermine
P51178
-
sphingosine 1-phosphate
Q9P212
sphingosine-1-phosphate stimulates PI-PLC activity of both splice variants
T cell antigen receptor
-
induces activation of PLC-gamma1, pathway
-
Trifluoperazine
-
the calmodulin antagonists activate at least one form of the rod outer segment enzyme by a calmodulin-independent mechanism
xanthurenic acid
-
triggers the hydrolysis of phosphoinositide by PI-PLC in gametocytes
Melittin
-
the calmodulin antagonists activate at least one form of the rod outer segment enzyme by a calmodulin-independent mechanism
additional information
-
rat brain protein kinase C phosphorylates phosphoinositidase gamma but not phosphoinositidase beta1. Incubation with the kinase increases the activity of both enzymes however, possibly by phosphorylation of another protein in the preparations
-
additional information
-
the enzyme is not stimulated by sodium deoxycholate
-
additional information
-
sodium lauryl sulfate, tetradecyl-trimethylammonium bromide, and Triton X-100 do not activate phosphatidylinositol phosphodiesterase in the presence of Ca2+
-
additional information
-
regulation of the PLC delta isoenzymes
-
additional information
-
regulation of the PLC delta isoenzymes, acrylonitrile causes a significant increase in PLC delta1 level in the heart and cytosol of cerebral cortex
-
additional information
-
modes of activation/activation pathways of the PLC isoenzymes beta, gamma, delta and epsilon
-
additional information
-
cleavage of GPI-anchored proteins by PI-PLC is influenced by bilayer fluidity, higher fluidity activates
-
additional information
-
membrane targeting of PLCgamma2 constructs containing either PLCdelta1 PH domain or the N-terminal tag of Lyn results in enzyme activation, phosphorylation of PLCgamma2 increases enzyme activity, raft targeting is essential for the B-cell antigen receptor and also for the H2O2 stress-induced 1D-myo-inositol 1,4,5-trisphosphate production in DT40 B-cell line
-
additional information
-
PLC-beta1 is not activated by phosphatidylserine, anionic and neutral detergents
-
additional information
-
extremely low frequency electromagnetic fields affect the phosphoinositide signal transduction pathway by triggering the PI-PLC activation and the consequent phosphatidylinositol 4,5-bisphosphate breakdown
-
additional information
Q99P84
study of PLCepsilon activation by several Ras mutants, recombinant purified PLCepsilon is not activated by Galpha-q, Gbetagamma or H-Ras in vitro
-
additional information
Q9TZN8
TcPI-PLC expression is induced during the differentiation of trypomastigotes into amastigotes
-
additional information
-
regulated by G protein subunits
-
additional information
Q6SA76, Q6TM09, Q6TM10
expression is induced by abiotic stress; expression is induced by abiotic stress; expression is induced by abiotic stress. The expression of Vr-PLC3 mRNA is unaffected by treatment with exogenous abscisic acid for 24 h. This raises the possibility that water and salt stress induction of Vr-PLC3 is mediated by an abscisic acid-independent pathway
-
additional information
Q9P212
treatment with 5-aza-2'deoxycytidine, an inhibitor of DNA methylation, results in an expression of PLCepsilon1b in tumor cells
-
additional information
P34024
activation by amphiphiles occurs with both micellar (phosphatidylinositol dispersed in detergents) and monomeric [dibutroylphosphatidylinositol (diC4PI)] phosphotransferase substrates and inositol 1,2-(cyclic)-phosphate (cIP), the phosphodiesterase substrate
-
additional information
-
the activation of PLCgamma2 is dependent on Syk and Src family kinases
-
additional information
P19174
recruitment of PLC-gamma1 to nephrin activated PLC-gamma1, as detected by phosphorylation of PLC-gamma1 Tyr-783 and increase in inositol 1,4,5-trisphosphate level
-
additional information
-
activation of PLC is essential for SDF-1-induced ERM (ezrin/radixin/moesin) protein release from cortical membrane and dephosphorylation
-
additional information
-
norepinephrine increases PLC beta1, beta3, gamma1, and delta1 isozyme gene expression; norepinephrine increases PLC beta1, beta3, gamma1, and delta1 isozyme gene expression; norepinephrine increases PLC beta1, beta3, gamma1, and delta1 isozyme gene expression; norepinephrine increases PLC beta1, beta3, gamma1, and delta1 isozyme gene expression
-
additional information
-
assay of the effect of Nod factors on in vitro PLC activity from nodule membrane fractions in the presence of guanosine 5'-[gamma-thio]triphosphate (GTPgammaS) and mastoparan
-
additional information
Q39032, Q39033, Q56W08, Q6NMA7, Q8GV43, Q944C1, Q944C2, Q9LY51, Q9STZ3
AtPLC is induced in response to salt, abscisic acid, cold and dehydration. Relative expression levels of AtPLC gene family in response to environmental stimuli is shown; AtPLC is induced in response to salt, abscisic acid, cold and dehydration. Relative expression levels of AtPLC gene family in response to environmental stimuli is shown; AtPLC is induced in response to salt, abscisic acid, cold and dehydration. Relative expression levels of AtPLC gene family in response to environmental stimuli is shown; AtPLC is induced in response to salt, abscisic acid, cold and dehydration. Relative expression levels of AtPLC gene family in response to environmental stimuli is shown; AtPLC is induced in response to salt, abscisic acid, cold and dehydration. Relative expression levels of AtPLC gene family in response to environmental stimuli is shown; AtPLC is induced in response to salt, abscisic acid, cold and dehydration. Relative expression levels of AtPLC gene family in response to environmental stimuli is shown; AtPLC is induced in response to salt, abscisic acid, cold and dehydration. Relative expression levels of AtPLC gene family in response to environmental stimuli is shown
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.1 - 0.15
1-phosphatidyl-1D-myo-inositol
-
-
0.006
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
25C, pH 7.4
0.0123
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
P51178
Vmax 58.8 nmol/min/ml without ARHGAP6 and with spermine
0.0197
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
P51178
Vmax 45.4 nmol/min/ml with ARHGAP6 and without spermine
0.023
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
Q9TZN8
pH 7.1, 30C
0.0232
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
P51178
Vmax 52.6 nmol/min/ml with ARHGAP6 and spermine
0.0391
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
P51178
Vmax 5.0 nmol/min/ml without ARHGAP6 and spermine
0.13
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
Q3ZTU7
recombinant enzyme
0.012
phosphatidylinositol
-
isoenzyme PLC-gamma
0.031
phosphatidylinositol
-
isoenzyme PLC-gamma
0.035
phosphatidylinositol
-
isoenzyme PLC-beta1
0.079
phosphatidylinositol
Q3ZTU7
recombinant enzyme
0.143
phosphatidylinositol
-
-
12.5
phosphatidylinositol
P34024
at the lower PI-PLC concentration (3.6 microg/ml)
100
phosphatidylinositol
P34024
at double the enzyme concentration
0.031
phosphatidylinositol 4,5-bisphosphate
-
-
0.058
phosphatidylinositol 4,5-bisphosphate
-
isoenzyme PLC-beta1
0.182
phosphatidylinositol 4,5-bisphosphate
-
phosphatidylinositide-specific phospholipase deltaDELTA1-134
0.031
phosphatidylinositol 4-phosphate
-
-
0.45
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
pH 7, PLC-beta1
additional information
additional information
-
kinetic study of 5-nucleotidase cleavage
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.001
phosphatidylserine
-
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.006
1-(6-((17beta-3-methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl)-1H-pyrrole-2,5-dione
-
significant inhibition of PLC-beta with IC50: 0.006 mM, little effect on PLC-beta1, PLC-beta3, or PLC-beta4
0.001
3beta-hydroxy-27-(E)-feruloyloxyolea-12-en-28-oic acid
-
in A549 cells, pH 7.0, 37C
0.0018
3beta-hydroxy-27-(E)-feruloyloxyolea-12-en-28-oic acid
-
in A549 cells, cytotoxic effects in log phase S, pH 7.0, 37C
0.0021
3beta-hydroxy-27-(E)-feruloyloxyolea-12-en-28-oic acid
-
in HCT-15 cells, cytotoxic effects in log phase S, pH 7.0, 37C; in HCT-15 cells, pH 7.0, 37C
0.003
3beta-hydroxy-27-(E)-feruloyloxyolea-12-en-28-oic acid
-
in MCF7 cells, pH 7.0, 37C
0.0054
3beta-hydroxy-27-(E)-feruloyloxyolea-12-en-28-oic acid
-
in HT-1 197 cells, pH 7.0, 37C
0.0182
3beta-hydroxy-27-(E)-feruloyloxyolea-12-en-28-oic acid
-
in P388 cells, pH 7.0, 37C
0.0357
3beta-hydroxy-27-(E)-feruloyloxyolea-12-en-28-oic acid
-
pH 7.0, 37C
0.0666
3beta-hydroxy-27-(E)-feruloyloxyolea-12-en-28-oic acid
-
in CCD-18Co cells, pH 7.0, 37C
0.0028
3beta-hydroxy-27-(E)-feruloyloxyurs-12-en-28-oic acid
-
in A549 cells, cytotoxic effects in log phase S, pH 7.0, 37C
0.0029
3beta-hydroxy-27-(E)-feruloyloxyurs-12-en-28-oic acid
-
in HCT-15 cells, cytotoxic effects in log phase S, pH 7.0, 37C; in HCT-15 cells, pH 7.0, 37C
0.0091
3beta-hydroxy-27-(E)-feruloyloxyurs-12-en-28-oic acid
-
in HT-1 197 cells, pH 7.0, 37C; in MCF7 cells, pH 7.0, 37C
0.01
3beta-hydroxy-27-(E)-feruloyloxyurs-12-en-28-oic acid
-
in A549 cells, pH 7.0, 37C
0.0143
3beta-hydroxy-27-(E)-feruloyloxyurs-12-en-28-oic acid
-
pH 7.0, 37C
0.0229
3beta-hydroxy-27-(E)-feruloyloxyurs-12-en-28-oic acid
-
in P388 cells, pH 7.0, 37C
0.0355
3beta-hydroxy-27-(E)-feruloyloxyurs-12-en-28-oic acid
-
in CCD-18Co cells, pH 7.0, 37C
0.0021
3beta-hydroxy-27-(Z)-feruloyloxyolea-12-en-28-oic acid
-
in HCT-15 cells, cytotoxic effects in log phase S, pH 7.0, 37C; in HCT-15 cells, pH 7.0, 37C
0.0027
3beta-hydroxy-27-(Z)-feruloyloxyolea-12-en-28-oic acid
-
in A549 cells, pH 7.0, 37C
0.0035
3beta-hydroxy-27-(Z)-feruloyloxyolea-12-en-28-oic acid
-
in HT-1 197 cells, pH 7.0, 37C
0.004
3beta-hydroxy-27-(Z)-feruloyloxyolea-12-en-28-oic acid
-
in MCF7 cells, pH 7.0, 37C
0.0065
3beta-hydroxy-27-(Z)-feruloyloxyolea-12-en-28-oic acid
-
in A549 cells, cytotoxic effects in log phase S, pH 7.0, 37C
0.0175
3beta-hydroxy-27-(Z)-feruloyloxyolea-12-en-28-oic acid
-
in P388 cells, pH 7.0, 37C
0.0446
3beta-hydroxy-27-(Z)-feruloyloxyolea-12-en-28-oic acid
-
pH 7.0, 37C
0.0503
3beta-hydroxy-27-(Z)-feruloyloxyolea-12-en-28-oic acid
-
in CCD-18Co cells, pH 7.0, 37C
0.0009
3beta-hydroxy-27-(Z)-feruloyloxyurs-12-en-28-oic acid
-
in MCF7 cells, pH 7.0, 37C
0.0038
3beta-hydroxy-27-(Z)-feruloyloxyurs-12-en-28-oic acid
-
in HCT-15 cells, cytotoxic effects in log phase S, pH 7.0, 37C; in HCT-15 cells, pH 7.0, 37C
0.0041
3beta-hydroxy-27-(Z)-feruloyloxyurs-12-en-28-oic acid
-
in A549 cells, cytotoxic effects in log phase S, pH 7.0, 37C
0.0043
3beta-hydroxy-27-(Z)-feruloyloxyurs-12-en-28-oic acid
-
in A549 cells, pH 7.0, 37C
0.0067
3beta-hydroxy-27-(Z)-feruloyloxyurs-12-en-28-oic acid
-
in HT-1 197 cells, pH 7.0, 37C
0.0229
3beta-hydroxy-27-(Z)-feruloyloxyurs-12-en-28-oic acid
-
in P388 cells, pH 7.0, 37C
0.0438
3beta-hydroxy-27-(Z)-feruloyloxyurs-12-en-28-oic acid
-
in CCD-18Co cells, pH 7.0, 37C
0.0441
3beta-hydroxy-27-(Z)-feruloyloxyurs-12-en-28-oic acid
-
pH 7.0, 37C
0.0836
3beta-hydroxy-27-4-(E)-2'-dihydrocoumaroyloxyurs-12-en-28-oic acid
-
pH 7.0, 37C
0.0008
3beta-hydroxy-27-4-(E)-coumaroyloxyolea-12-en-28-oic acid
-
in HCT-15 cells, cytotoxic effects in log phase S, pH 7.0, 37C; in HCT-15 cells, pH 7.0, 37C
0.0019
3beta-hydroxy-27-4-(E)-coumaroyloxyolea-12-en-28-oic acid
-
in MCF7 cells, pH 7.0, 37C
0.002
3beta-hydroxy-27-4-(E)-coumaroyloxyolea-12-en-28-oic acid
-
in A549 cells, cytotoxic effects in log phase S, pH 7.0, 37C
0.0037
3beta-hydroxy-27-4-(E)-coumaroyloxyolea-12-en-28-oic acid
-
in HT-1 197 cells, pH 7.0, 37C
0.0066
3beta-hydroxy-27-4-(E)-coumaroyloxyolea-12-en-28-oic acid
-
in A549 cells, pH 7.0, 37C
0.0186
3beta-hydroxy-27-4-(E)-coumaroyloxyolea-12-en-28-oic acid
-
in P388 cells, pH 7.0, 37C
0.022
3beta-hydroxy-27-4-(E)-coumaroyloxyolea-12-en-28-oic acid
-
pH 7.0, 37C
0.0524
3beta-hydroxy-27-4-(E)-coumaroyloxyolea-12-en-28-oic acid
-
in CCD-18Co cells, pH 7.0, 37C
0.0009
3beta-hydroxy-27-4-(E)-coumaroyloxyurs-12-en-28-oic acid
-
in MCF7 cells, pH 7.0, 37C
0.0024
3beta-hydroxy-27-4-(E)-coumaroyloxyurs-12-en-28-oic acid
-
in A549 cells, cytotoxic effects in log phase S, pH 7.0, 37C
0.0038
3beta-hydroxy-27-4-(E)-coumaroyloxyurs-12-en-28-oic acid
-
in A549 cells, pH 7.0, 37C
0.0045
3beta-hydroxy-27-4-(E)-coumaroyloxyurs-12-en-28-oic acid
-
in HCT-15 cells, cytotoxic effects in log phase S, pH 7.0, 37C; in HCT-15 cells, pH 7.0, 37C
0.0051
3beta-hydroxy-27-4-(E)-coumaroyloxyurs-12-en-28-oic acid
-
in HT-1 197 cells, pH 7.0, 37C
0.0095
3beta-hydroxy-27-4-(E)-coumaroyloxyurs-12-en-28-oic acid
-
pH 7.0, 37C
0.0226
3beta-hydroxy-27-4-(E)-coumaroyloxyurs-12-en-28-oic acid
-
in P388 cells, pH 7.0, 37C
0.0614
3beta-hydroxy-27-4-(E)-coumaroyloxyurs-12-en-28-oic acid
-
sulforhodamine B, cytotoxic effects of 3beta-hydroxy-27-4-(E)-coumaroyloxyurs-12-en-28-oic acid on the HCT-15 cell line by the sulforhodamine B (SRB) and colony forming assays, pH 7.0, 37C
0.065
3beta-hydroxy-27-4-(E)-coumaroyloxyurs-12-en-28-oic acid
-
in CCD-18Co cells, pH 7.0, 37C
0.0737
3beta-hydroxy-27-4-(E)-coumaroyloxyurs-12-en-28-oic acid
-
colony-forming, cytotoxic effects of 3beta-hydroxy-27-4-(E)-coumaroyloxyurs-12-en-28-oic acid on the HCT-15 cell line by the sulforhodamine B (SRB) and colony forming assays, pH 7.0, 37C
0.1213
3beta-hydroxy-27-4-(E)-coumaroyloxyurs-12-en-28-oic methyl ester
-
pH 7.0, 37C
0.0017
3beta-hydroxy-27-4-(Z)-coumaroyloxyolea-12-en-28-oic acid
-
in HT-1 197 cells, pH 7.0, 37C
0.0021
3beta-hydroxy-27-4-(Z)-coumaroyloxyolea-12-en-28-oic acid
-
in A549 cells, cytotoxic effects in log phase S, pH 7.0, 37C; in HCT-15 cells, cytotoxic effects in log phase S, pH 7.0, 37C; in HCT-15 cells, pH 7.0, 37C; in MCF7 cells, pH 7.0, 37C
0.0032
3beta-hydroxy-27-4-(Z)-coumaroyloxyolea-12-en-28-oic acid
-
in A549 cells, pH 7.0, 37C
0.0174
3beta-hydroxy-27-4-(Z)-coumaroyloxyolea-12-en-28-oic acid
-
in P388 cells, pH 7.0, 37C
0.0277
3beta-hydroxy-27-4-(Z)-coumaroyloxyolea-12-en-28-oic acid
-
pH 7.0, 37C
0.059
3beta-hydroxy-27-4-(Z)-coumaroyloxyolea-12-en-28-oic acid
-
in CCD-18Co cells, pH 7.0, 37C
0.0014
3beta-hydroxy-27-4-(Z)-coumaroyloxyurs-12-en-28-oic acid
-
in MCF7 cells, pH 7.0, 37C
0.0043
3beta-hydroxy-27-4-(Z)-coumaroyloxyurs-12-en-28-oic acid
-
in HCT-15 cells, cytotoxic effects in log phase S, pH 7.0, 37C; in HCT-15 cells, pH 7.0, 37C
0.0047
3beta-hydroxy-27-4-(Z)-coumaroyloxyurs-12-en-28-oic acid
-
in A549 cells, cytotoxic effects in log phase S, pH 7.0, 37C
0.0072
3beta-hydroxy-27-4-(Z)-coumaroyloxyurs-12-en-28-oic acid
-
in HT-1 197 cells, pH 7.0, 37C
0.0076
3beta-hydroxy-27-4-(Z)-coumaroyloxyurs-12-en-28-oic acid
-
in A549 cells, pH 7.0, 37C
0.0245
3beta-hydroxy-27-4-(Z)-coumaroyloxyurs-12-en-28-oic acid
-
pH 7.0, 37C
0.0245
3beta-hydroxy-27-4-(Z)-coumaroyloxyurs-12-en-28-oic acid
-
in P388 cells, pH 7.0, 37C
0.0733
3beta-hydroxy-27-4-(Z)-coumaroyloxyurs-12-en-28-oic acid
-
in CCD-18Co cells, pH 7.0, 37C
0.0001
adriamycin
-
in HT-1 197 cells, pH 7.0, 37C
0.0004
adriamycin
-
colony-forming, cytotoxic effects of 3beta-hydroxy-27-4-(E)-coumaroyloxyurs-12-en-28-oic acid on the HCT-15 cell line by the sulforhodamine B (SRB) and colony forming assays, pH 7.0, 37C
0.0007
adriamycin
-
colony-forming, cytotoxic effects of 3beta-hydroxy-27-4-(E)-coumaroyloxyurs-12-en-28-oic acid on the A549 cell line by the sulforhodamine B (SRB) and colony forming assays, pH 7.0, 37C
0.0013
adriamycin
-
in A549 cells, cytotoxic effects in log phase S, pH 7.0, 37C; in A549 cells, pH 7.0, 37C
0.0101
adriamycin
-
in CCD-18Co cells, pH 7.0, 37C
0.0246
adriamycin
-
in HCT-15 cells, pH 7.0, 37C; in HT-1 197 cells, cytotoxic effects in log phase S, pH 7.0, 37C; sulforhodamine B, cytotoxic effects of 3beta-hydroxy-27-4-(E)-coumaroyloxyurs-12-en-28-oic acid on the HCT-15 cell line by the sulforhodamine B (SRB) and colony forming assays, pH 7.0, 37C
0.003
U73122
-
pH and temperature not specified in the publication
0.029
amentoflavone
-
pH 7.0, 37C
additional information
additional information
-
IC50 value is less than 0.1 microM for adriamycin in MCF7 cells, pH 7.0, 37C; the IC50 values for 3beta,7'-diacetoxy-27-4-(E)-coumaroyloxyurs-12-en-28-oic acid, 3beta-hydroxyurs-12-en-28-oic 4-(E)-coumaroyl ester, 3beta,27-dihydroxyurs-12-en-28-oic acid, 3beta-4-(E)-coumaroyloxyurs-12-en-28-oic acid, and 4-(E)-coumaric acidare above 0.250 mM, pH 7.0, 37C
-
additional information
additional information
-
IC50 value is less than 0.1 microM for adriamycin in in P388 cells, pH 7.0, 37C
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.011
-
digestive gland
0.019
-
mussel gill
0.025
-
pH 7.3, pellet
0.027
-
specific activity of 20-27 nmol/mg/min in membrane fractions of nodules 18-20 days after inoculation with Rhizobium tropici. In the presence of 5 mM mastoparan, PIP2-PLC activity is almost double the basal level
0.034
-
pH 5.4, pellet
0.082
-
pH 7.3, cell pellet
0.088
-
pH 5.4, cell pellet
0.114
-
membrane-bound enzyme
0.235
-
cytosolic enzyme
0.26
-
pH 7.3, supernatant (soluble)
0.28
-
pH 5.4, supernatant (soluble)
0.5784
-
-
0.7
P34024
dibutroylphosphatidylinositol as substrate
1.2
P34024
dibutroylphosphatidylinositol as substrate, dihexanoylphosphatidylcholine as amphiphile; dibutroylphosphatidylinositol as substrate, KCl 0.075 M
1.3
Q8K4D7, Q8R3B1
at 0.001 mM Ca2+; PLCzeta, at 0.001 mM Ca2+, pH 7, 37C
1.3
P34024
phosphatidylinositol as substrate
1.8
P34024
dibutroylphosphatidylinositol as substrate, Triton X-100 as amphiphile
3.1
-
isoenzyme PLC-gamma
9
P34024
phosphatidylinositol as substrate, 1-palmitoyl-2-oleoylphosphatidylcholine as amphiphile
10
P34024
dibutroylphosphatidylinositol as substrate, dihexanoylphosphatidylcholine as amphiphile, KCl 0.075 M
11.2
P34024
dibutroylphosphatidylinositol as substrate, Triton X-100 as amphiphile, KCl 0.075 M
11.4
-
isoenzyme PLC-beta1
12.6
-
pH 7, 37C, hydrolysis of phosphatidylinositol 4,5-bisphosphate
28.97
-
phosphatidylinositol as substrate, last purification step
32.7
P34024
1.6 microg/ml PI-PLC, citraconic anhydride modification 2 (chemical modification with citraconic anhydride changes the positive charges of lysine residues on the protein surface to negative ones)
54
P34024
phosphatidylinositol as substrate, KCl 0.10 M
60.6
P34024
1.6 microg/ml PI-PLC, control
65
Q8K4D7, Q8R3B1
at 0.03 mM Ca2+, PLC-delta1; PLCdelta1, at 0.03 mM Ca2+, pH 7, 37C
82.5
P34024
1.6 microg/ml PI-PLC, citraconic anhydride modification 1 (chemical modification with citraconic anhydride changes the positive charges of lysine residues on the protein surface to negative ones)
109
P34024
0.2 microg/ml PI-PLC, citraconic anhydride modification 2 (chemical modification with citraconic anhydride changes the positive charges of lysine residues on the protein surface to negative ones)
127
P34024
phosphatidylinositol as substrate, 1-palmitoyl-2-oleoylphosphatidylcholine as amphiphile, (NH4)2SO4 0.10 M
136
-
HeLa S3 cells; hydrolysis of phosphatidylinositol 4,5-bisphosphate
165
P34024
0.2 microg/ml PI-PLC, citraconic anhydride modification 1 (chemical modification with citraconic anhydride changes the positive charges of lysine residues on the protein surface to negative ones)
204.1
-
phosphatidylinositol 4,5-bisphosphate as substrate, last purification step
274
P34024
phosphatidylinositol as substrate, Triton X-100 as amphiphile
303
P34024
0.2 microg/ml PI-PLC, control
330
P34024
phosphatidylinositol as substrate, KCl 0.20 M
403
P34024
0.2 microg/ml PI-PLC, 0.15 M KCl, citraconic anhydride modification 2 (chemical modification with citraconic anhydride changes the positive charges of lysine residues on the protein surface to negative ones)
511
P34024
phosphatidylinositol as substrate, KCl 0.30 M
534
P34024
0.2 microg/ml PI-PLC, citraconic anhydride modification 2 (chemical modification with citraconic anhydride changes the positive charges of lysine residues on the protein surface to negative ones)
936
P34024
phosphatidylinositol as substrate, Triton X-100 as amphiphile, NH4Cl 0.15 M
990
P34024
0.2 microg/ml PI-PLC, 0.15 M KCl, citraconic anhydride modification 1 (chemical modification with citraconic anhydride changes the positive charges of lysine residues on the protein surface to negative ones)
1030
P34024
0.2 microg/ml PI-PLC, 0.15 M KCl, control; phosphatidylinositol as substrate, Triton X-100 as amphiphile, KCl 0.15 M
1120
P34024
phosphatidylinositol as substrate, Triton X-100 as amphiphile, K2SO4 0.10 M
1150
P34024
0.2 microg/ml PI-PLC, control
1430
P34024
phosphatidylinositol as substrate, Triton X-100 as amphiphile, (NH4)2SO4 0.10 M
additional information
-
PLC activity in vascular smooth muscle cells can be divided into two type based on their calcium and pH requirements, substrate preferences, and molecular weights
additional information
-
enzyme specific activity is higher in cytosol prepared from soleus muscle than in that from extensor digitorum longus muscle
additional information
-
at a substrate concentration of 50 microM the rate of hydrolysis is 0.3 nmol/h per mg of tissue (wet weight) at pH 6.6
additional information
-
activity at pH 7.0 (and 0.4 mM CaCl2) is decreased by agents, such as salts and lipophilic cations, which tend to neutralize the negative charge of phosphatidylinositol. At pH 5.5 these agents slightly stimulate activity
additional information
-
PLC beta isoenzymes, activities in membrane deprived and intact nuclei
additional information
Q39033
60-350times higher specific activity with phosphatidylinositol 4,5-bisphosphate than with phosphatidylinositol as substrate
additional information
Q9TZN8
-
additional information
P16885
-
additional information
P34024
measured using 31P NMR spectroscopy, loss of activity with high enzyme concentrations. Specific activity of the enzyme can be enhanced by dilution of the protein or by addition of both zwitterionic and neutral amphiphiles (e.g., diheptanoylphosphatidylcholine or Triton X-100) or 0.1-0.2 M inorganic salts
additional information
-
analysis of PI-PLC product levels upon gametocyte activation, overview
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
4.5 - 5.5
-
type I, II, and III. The major form (III) also has substantial activity at pH 7.0 and above 4
5 - 5.5
-
hydrolysis of phosphatidylinositol, isoenzyme PLC-beta-1 and PLC-gamma, in presence of 1 mM CaCl2
5 - 5.5
-
extensive activity in the alkaline range (7-8.5) as well as at pH 5-5.5
5
-
high molecular PLC (157 kDa), phosphatidylinositol as substrate
5.4 - 5.6
-
5.4-5.6 and 7.0-7.3
5.5 - 6
-
hydrolysis of phosphatidylinositol 4,5-bisphosphate, membrane-bound enzyme
5.5
-
two optima 5.5 and 7.4
5.5
-
two optima pH 5.5 and pH 7.4
5.5
-
two pH optima are apparent, at pH 5.5 and pH 7.4
5.5
-
assay at
5.6
-
when (NH4)2SO4 fractionation (30-50%) of the brain supernatant is carried out, the phosphodiesterase preparation obtained shows an optimum at pH 5.6
5.6
-
assay at
6
-
hydrolysis of phosphatidylinositol, membrane bound enzyme and cytosolic enzyme
6
-
at the higher calcium concentration the pH optimum for phosphatidylinositol 4,5-bisphosphate hydrolysis is 6.0
6.2
O24297
assay at
6.5 - 7
-
hydrolysis of phosphatidylinositol, isoenzyme PLC-beta1 in presence of 0.6 mg/ml sodium deoxycholate, isoenzyme PLC-gamma in presence of 0.4 mg/ml sodium deoxycholate
6.5
-
hydrolysis of phosphatidylinositol 4,5-bisphosphate, in presence of sodium deoxycholate, enzyme from plasma membrane
6.6
-
assay at, phosphatidylinositol 4,5-bisphosphate as substrate
6.7 - 6.8
Q9TZN8
-
6.7
Q9NQ66
assay at
6.7
Q9Z1B3
assay at
6.7
P10687
assay at
6.8
-
hydrolysis of phosphatidylinositol 4,5-diphosphate
6.8
-
brain enzyme
7 - 7.3
-
5.4-5.6 and 7.0-7.3
7 - 7.5
-
pH 7.0: about 30% of maximal activity, pH 7.5: about 75% of maximal activity, hydrolysis of phosphatidylinositol 4,5-diphosphate
7 - 8.5
-
extensive activity in the alkaline range (7-8.5) as well as at pH 5-5.5
7
-
assay at
7
-
Pi-PLC (cytosol); Pi-PLC (membrane) solubilized with sodium cholate
7
-
assay at
7
Q8K4D7, Q8R3B1
assay at
7
-
maximal activity in the pH-range 6.5-7.5 with a peak at pH 7
7
A2AP18
-
7.1
-
assay at
7.2 - 7.5
Q3ZTU7
hydrolysis of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
7.3
-
hydrolysis of phosphatidylinositol 4,5-bisphosphate, in presence of sodium deoxycholate, enzyme from cytoplasm
7.4
-
assay at
7.4
-
platelet enzyme
7.4
-
two optima 5.5 and 7.4
7.4
-
two optima pH 5.5 and pH 7.4
7.4
-
two pH optima are apparent, at pH 5.5 and pH 7.4
7.5
-
hyrolysis of phosphatidylinositol 4,5-diphosphate, membrane bound enzyme and cytosolic enzyme
7.5
-
hydrolysis of phosphatidylinositol 4,5-diphosphate, in presence of 0.1 mM CaCl2
7.8
-
assay at
7.8
-
assay at, phosphatidylinositol as substrate
8
-
Pi-PLC (membrane) solubilized with octyl glucoside
8
Q3ZTU7
hydrolysis of phosphatidylinositol
additional information
-
activity at higher pH values requires higher calcium concentrations and disappears on purification of the soluble enzyme by ammonium sulfate fractionation
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
4.5 - 7
-
pH 4.5: about 50% of maximal activity, pH 7.0: about 40% of maximal activity
4.5 - 7.5
-
pH 4.5: about 25% of maximal activity, pH 7.5: about 55% of maximal activity, hydrolysis of phosphatidylinositol 4,5-diphosphate
4.5 - 7.5
-
-
5.3 - 7.7
-
about 45% of maximal activity at pH 5.3 and pH 7.7
5.7 - 8
-
pH 5.7: about 70% of maximal activity, pH 8.0: about 50% of maximal activity
6 - 11
-
pH: 6.0: about 10% of maximal activity, pH 11.0: about 40% of maximal activity
6 - 7
-
-
6 - 7.5
-
about 75% of maximal activity at pH 6.0 and 7.5
6.4 - 7.8
-
pH 6.4: about 55% of maximal activity, pH 7.8: about 20% of maximal activity
6.5 - 7.3
Q9TZN8
enzyme activity decreases very rapidly below pH 6.5 and above pH 7.25
6.5 - 7.5
-
maximal activity in the pH-range 6.5-7.5 with a peak at pH 7
7.1 - 7.6
Q3ZTU7
pH 7.1: about 85% of maximal activity, pH 7.6: about 65% of maximal activity, hydrolysis of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
7.1 - 9.2
Q3ZTU7
pH 7.1: about 90% of maximal activity, pH 9.2: about 85% of maximal activity, hydrolysis of phosphatidylinositol
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
20
Q3ZTU7
hydrolysis of phosphatidylinositol
25
O24297
assay at
30
Q9TZN8
assay at
30
Q3ZTU7
hydrolysis of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
37
-
assay at
37
-
assay at
37
Q8K4D7, Q8R3B1
assay at
37
-
assay at
37
-
assay at
37
Q9NQ66
assay at
37
Q9Z1B3
assay at
37
P10687
assay at
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
5 - 30
Q3ZTU7
5C: about 50% of maximal activity, 30C: about 45% of maximal activity, hydrolysis of phosphatidylinositol
15 - 37
Q3ZTU7
15C: about 75% of maximal activity, 37C: about 80% of maximal activity, hydrolysis of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
37 - 60
-
37C: optimal temperature, 60C: 27% of maximal activity
pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
3.8 - 4.2
-
4 peaks of phosphodiesterase activity are found at pl ranges 7.2-7.4 , 5.8-6.0, 4.4-4.8 and 3.8-4.2, pH 5.5
4.4 - 4.8
-
4 peaks of phosphodiesterase activity are found at pl ranges 7.2-7.4 , 5.8-6.0, 4.4-4.8 and 3.8-4.2, pH 5.5
4.6
-
when assayed at pH 7.0 and pH 8.0, 1 mM Ca2+
5.31
B2LSE7
sequence analysis
5.86
-
4 peaks of phosphodiesterase activity are found at pl ranges 7.2-7.4 , 5.8-6.0, 4.4-4.8 and 3.8-4.2, pH 5.5
6.2
-
purified Pi-PLC (cytosol)
7.2 - 7.4
-
4 peaks of phosphodiesterase activity are found at pl ranges 7.2-7.4 , 5.8-6.0, 4.4-4.8 and 3.8-4.2, pH 5.5
9 - 9.2
-
purified Pi-PLC (membrane)
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
-
expression of PLC delta2
Manually annotated by BRENDA team
-
bovine adrenal chromaffin cells
Manually annotated by BRENDA team
Q9TZN8
TcPI-PLC expression at high levels in the epimastigote and amastigote stages
Manually annotated by BRENDA team
Q9NQ66
highest expression level of PLCbeta1 in brain, especially in the amygdala, caudate nucleus and hippocampus
Manually annotated by BRENDA team
-
anaplastic, PLC-eta2, PLC-eta2
Manually annotated by BRENDA team
-
PLC-gamma2 mRNA is expressed in the limited areas of anterior pituitary and cerebellar Purkinje and granule cells
Manually annotated by BRENDA team
-
PLC delta3 and delta4
Manually annotated by BRENDA team
-
adult brain, PLC-delta1 is concentrated in astroglial cells, much lower levels in neurons
Manually annotated by BRENDA team
Q62711, Q99P84, Q9QW07
PI-PLC delta3
Manually annotated by BRENDA team
-
PLC-gamma1 mRNA is widely detected in various tissues. It is abundantly expressed in embryonal cortical structures, neurons, oligodendrocytes and astrocytes
Manually annotated by BRENDA team
-
PI-PLC expression pattern, overview
Manually annotated by BRENDA team
-
mechanosensitive activation of K+ channel via phospholipase C-induced depletion of phosphatidylinositol 4,5-bisphosphate
Manually annotated by BRENDA team
-
homogenates, total particulate and soluble fractions
Manually annotated by BRENDA team
Q9NQ66
adult, highest expression level of PLCbeta1 in brain, especially in the amygdala, caudate nucleus and hippocampus
Manually annotated by BRENDA team
-
adult, PLC-gamma1 is diffusely distributed, expression pattern of PLC-gamma1 and PLC-delta1 during development, expression pattern of PLC-gamma2
Manually annotated by BRENDA team
-
PLC delta1, delta3 and delta4
Manually annotated by BRENDA team
-
PLC-beta1
Manually annotated by BRENDA team
-
PLC-eta1, PLC-eta2
Manually annotated by BRENDA team
A2AP18
PLCeta2 is restricted to brain. PLCeta2 gradually increases during brain development. PLCeta2 is not detected in embryos. At 1 week after birth PLCeta2 is barely detected and expression is first observed at weeks after birth
Manually annotated by BRENDA team
-
involvement of brain phosphatidylinositol-specific phospholipase C in the centrally administered histamine-induced activation of the adrenomedullary outflow in rats
Manually annotated by BRENDA team
-
PLC-gamma1, PLC-delta3, PLC-delta1, PLC-delta4 and PLC-eta mRNA is expressed in. PLC-beta3 is found in the brain, liver, and parotid gland
Manually annotated by BRENDA team
-
cerebral hemispheres
Manually annotated by BRENDA team
-
frontal cortex, single inescapable shock reduces PI-PLC activity in the frontal cortex and hippocampus of learned helplessness rats. Repeated inescapable shock not only reduced PI-PLC activity but also selectively decreases the expression of PLCbeta1 and PLCgamma1 isozymes
Manually annotated by BRENDA team
-
differentiation of C2C12 myoblasts in response to mitogen withdrrawal and insulin stimulation is characterized by a marked increase in nuclear PI-PLCbeta1
Manually annotated by BRENDA team
Q62711, Q99P84, Q9QW07
astrocytoma, PI-PLC delta3
Manually annotated by BRENDA team
-
20 days old calli
Manually annotated by BRENDA team
-
PLC delta3 and delta4
Manually annotated by BRENDA team
-
the carotid bodies (CB) are a paired chemoreceptor organ located at the bifurcation of the common carotid arteries
Manually annotated by BRENDA team
Q9NQ66
highest expression level of PLCbeta1 in brain, especially in the amygdala, caudate nucleus and hippocampus
Manually annotated by BRENDA team
-
isoenzyme PI-PLC-beta1
Manually annotated by BRENDA team
-
PLC-gamma2 mRNA is expressed in the limited areas of anterior pituitary and cerebellar Purkinje and granule cells
Manually annotated by BRENDA team
-
PLC-beta4 is present at the highest level in the cerebellum and retina
Manually annotated by BRENDA team
-
expression of PLC delta2
Manually annotated by BRENDA team
A2AP18
PLCeta2 is restricted to brain. PLCeta2 gradually increases during brain development. PLCeta2 is not detected in embryos. At 1 week after birth PLCeta2 is barely detected and expression is first observed at weeks after birth
Manually annotated by BRENDA team
-
PLC-beta1 is highly expressed in the cerebral cortex and hippocampus
Manually annotated by BRENDA team
-
PLC-eta mRNA is expressed in colon
Manually annotated by BRENDA team
Mytilus galloprovincialis Lam
-
-
-
Manually annotated by BRENDA team
-
high PLC-beta3 expression in very early embryos
Manually annotated by BRENDA team
-
PLC-gamma homolog, widely distributed in the embryo
Manually annotated by BRENDA team
-
PLC-gamma1, PLC-gamma1 mRNA is widely detected in various tissues. It is abundantly expressed in embryonal cortical structures, neurons, oligodendrocytes and astrocytes
Manually annotated by BRENDA team
Q9TZN8
TcPI-PLC expression at high levels in the epimastigote and amastigote stages
Manually annotated by BRENDA team
-
homogenate
Manually annotated by BRENDA team
-
NorpA type I, eye-specific
Manually annotated by BRENDA team
-
PLC-eta1
Manually annotated by BRENDA team
-
W-138 fibroblasts, expression of PLC delta isoenzymes
Manually annotated by BRENDA team
Q39033
AtPLC2 expression
Manually annotated by BRENDA team
-
constitutive expression of AtPLC2 in vegetative and floral tissues
Manually annotated by BRENDA team
Mytilus galloprovincialis Lam
-
-
-
Manually annotated by BRENDA team
-
C6Bu-1 rat glioma cell, isoenzyme PI-PLC-beta1
Manually annotated by BRENDA team
-
PLC-gamma2 mRNA is expressed in the limited areas of anterior pituitary and cerebellar Purkinje and granule cells
Manually annotated by BRENDA team
-
macrophage cell line
Manually annotated by BRENDA team
-
chicken HD11 macrophage cell
Manually annotated by BRENDA team
-
PLC-21 type I, head-specific
Manually annotated by BRENDA team
-
upper head, PLC-eta1
Manually annotated by BRENDA team
-
coronary artery smooth muscle
Manually annotated by BRENDA team
Q99P84
expression of PLCepsilon
Manually annotated by BRENDA team
-
PLC-epsilon, most abundantly in
Manually annotated by BRENDA team
-
highest expression of PLC-eta detected in heart. PLC-delta3 is present in heart. PLC-delta1 is present in high abundancy in the brain, heart, lung, skeletal muscle and testis
Manually annotated by BRENDA team
Rattus norvegicus PLCepsilon
-
expression of PLCepsilon
-
Manually annotated by BRENDA team
Q9P212
PLC epsilon1b is only expressed in HEK293T cells
Manually annotated by BRENDA team
-
limited expression of PLC-beta2 in hematopoietic cells
Manually annotated by BRENDA team
-
isoenzyme PI-PLC-beta1
Manually annotated by BRENDA team
-
rapidly growing hepatoma 3924A
Manually annotated by BRENDA team
Q9NQ66
highest expression level of PLCbeta1 in brain, especially in the amygdala, caudate nucleus and hippocampus
Manually annotated by BRENDA team
-
PLC-beta1 is prominently expressed in hippocampal pyramidal cells
Manually annotated by BRENDA team
A2AP18
PLCeta2 is restricted to brain. PLCeta2 gradually increases during brain development. PLCeta2 is not detected in embryos. At 1 week after birth PLCeta2 is barely detected and expression is first observed at weeks after birth
Manually annotated by BRENDA team
-
single inescapable shock reduces PI-PLC activity in the frontal cortex and hippocampus of learned helplessness rats. Repeated inescapable shock not only reduced PI-PLC activity but also selectively decreases the expression of PLCbeta1 and PLCgamma1 isozymes
Manually annotated by BRENDA team
-
PLC-beta1 is highly expressed in the cerebral cortex and hippocampus
Manually annotated by BRENDA team
-
promyelocytic cell line, undifferentiated and DMSO-differentiated
Manually annotated by BRENDA team
-
isoenzyme PI-PLC-1
Manually annotated by BRENDA team
-
phosphorylation of nuclear PLC-beta1b on serine occurs at the G2/M and the late G1 phase and is necessary for the progression of the cells through the cell cycle
Manually annotated by BRENDA team
-
human umbilical vein endothelial cell
Manually annotated by BRENDA team
-
TRPV6-expressing cell
Manually annotated by BRENDA team
-
PLC delta3 and delta4
Manually annotated by BRENDA team
-
PLC-delta4 mRNA is expressed in various tissues with the highest levels detected selectively in the brain, skeletal muscle, testis and kidney
Manually annotated by BRENDA team
Q6QJ78
-
Manually annotated by BRENDA team
-
outer leaves
Manually annotated by BRENDA team
-
AtPLC1S is concentrated in shoot and leaf
Manually annotated by BRENDA team
Q39033
AtPLC2 expression
Manually annotated by BRENDA team
Q9FSW1
DsPLC2, from 6 weeks old plants
Manually annotated by BRENDA team
Q6SA76, Q6TM09, Q6TM10
the Vr-PLC3 protein is specifically activated by drough and salt stress in an abscisic acid-independent manner, with its induction being faster in roots than in leaf tissue
Manually annotated by BRENDA team
O24297
phosphatidylinositol-4,5-bisphosphate-specific phospholipase C appears to play a key role in free salicylic acid and abscisic acid-associated reinforcement of thermotolerance resulting from heat acclimation
Manually annotated by BRENDA team
Q39032, Q39033, Q56W08, Q6NMA7, Q8GV43, Q944C1, Q944C2, Q9LY51, Q9STZ3
AtPLC3 expresses to higher transcript levels in leaves and stems as compared to flowers and roots
Manually annotated by BRENDA team
Q99P84
expression of PLCepsilon
Manually annotated by BRENDA team
-
PLC delta3 and delta4
Manually annotated by BRENDA team
-
an early increase in PLC-beta1b activity (6 h after partial hepatectomy) in nuclear matrix is associated with serine phosphorylation of the enzyme, the later increase (20 h after partial hepatectomy) paralleles the increase in the amount of protein, the activity of PLC-delta1 and the amount of protein found in chromatin is increased only at 20 h after partial hepatectomy, the increase in the PLC-gamma1 activity measured at 6 h and 20 h after partial hepatectomy is associated with tyrosine phosphorylation of the enzyme
Manually annotated by BRENDA team
-
PLC-beta3 is found in the brain, liver, and parotid gland
Manually annotated by BRENDA team
Rattus norvegicus PLCepsilon
-
expression of PLCepsilon
-
Manually annotated by BRENDA team
Q99P84
expression of PLCepsilon
Manually annotated by BRENDA team
Q9P212
PLC epsilon1b is enriched in
Manually annotated by BRENDA team
-
PLC-eta mRNA is expressed in lung. PLC-delta1 is present in high abundancy in the brain, heart, lung, skeletal muscle and testis
Manually annotated by BRENDA team
Rattus norvegicus PLCepsilon
-
expression of PLCepsilon
-
Manually annotated by BRENDA team
-
PLC-eta1, PLC-eta2
Manually annotated by BRENDA team
-
PLC-gamma2
Manually annotated by BRENDA team
-
mesenteric lymph node
Manually annotated by BRENDA team
-
from mesenteric lymph nodes
Manually annotated by BRENDA team
-
breast cancer cell line
Manually annotated by BRENDA team
-
compared with the G0/G1 phases of the cell cycle, the cells in S/G2/M phases show high PLC-beta2 expressions that reach the greatest levels during the late mitotic stages. Phospholipase C-beta2 promotes mitosis and migration of human breast cancer-derived cells
Manually annotated by BRENDA team
-
compared with the G0/G1 phases of the cell cycle, the cells in S/G2/M phases show high PLC-b2 expressions that reach the greatest levels during the late mitotic stages. Phospholipase C-beta2 promotes mitosis and migration of human breast cancer-derived cells
Manually annotated by BRENDA team
-
pSuperior 3Mut and pSuperior shPLCgamma1
Manually annotated by BRENDA team
-
cultured vascular smooth muscle cell, isolated from rat thoracic aorta
Manually annotated by BRENDA team
-
prepared from rat slow (soleus) and fast (extensor digitorum longus) muscles
Manually annotated by BRENDA team
-
C2C12 rat myoblast, isoenzyme PI-PLC-beta1
Manually annotated by BRENDA team
P10687
neonatal rat ventricular myocyte, NRVM
Manually annotated by BRENDA team
-
PLC-beta2 may be responsible, by modifying the phosphoinositide pools, for the changes of cytoskeleton architecture that take place during the acquisition of migration capability of differentiating promyelocytes
Manually annotated by BRENDA team
-
PLC-eta1, PLC-eta2
Manually annotated by BRENDA team
-
adult brain, much lower levels of PLC-delta1 in neurons than in astroglial cells
Manually annotated by BRENDA team
-
PLC-gamma1 mRNA is widely detected in various tissues. It is abundantly expressed in embryonal cortical structures, neurons, oligodendrocytes and astrocytes
Manually annotated by BRENDA team
-
fibroblasts, treated with insulin-like growth factor 1
Manually annotated by BRENDA team
-
isoenzyme PI-PLC-1
Manually annotated by BRENDA team
-
at G1/S boundary of the cell cycle and during quiescence, nuclear PLCbeta1 activity is up-regulated two-fold in Swiss 3T3 fibroblasts stimulated with IGF-I
Manually annotated by BRENDA team
-
PLCgamma2 is the predominant isoform
Manually annotated by BRENDA team
A2AP18
PLCeta2 is restricted to brain. PLCeta2 gradually increases during brain development. PLCeta2 is not detected in embryos. At 1 week after birth PLCeta2 is barely detected and expression is first observed at weeks after birth
Manually annotated by BRENDA team
-
PLC-gamma1 mRNA is widely detected in various tissues. It is abundantly expressed in embryonal cortical structures, neurons, oligodendrocytes and astrocytes
Manually annotated by BRENDA team
-
high PLC-beta3 expression in unfertilized oocytes
Manually annotated by BRENDA team
-
isoenzyme PI-PLC-1
Manually annotated by BRENDA team
-
PLCbeta1 is first exclusively localized to the nucleus and then migrates to the cytoplasm when the oocyte is fully grown. During oocyte maturation PLCbeta1 is active in the cytoplasm before it migrates and becomes active in the nucleus just prior to germinal vesicle breakdown
Manually annotated by BRENDA team
-
PLC-eta1, PLC-eta2
Manually annotated by BRENDA team
-
PLC-delta3
Manually annotated by BRENDA team
-
PLC-eta1, PLC-eta2
Manually annotated by BRENDA team
-
PLC-beta3 is found in the brain, liver, and parotid gland
Manually annotated by BRENDA team
-
expression of PLC delta
Manually annotated by BRENDA team
-
PLC-eta1, PLC-eta2
Manually annotated by BRENDA team
Q9P212
PLC epsilon1b is enriched in
Manually annotated by BRENDA team
-
PLC-beta4
Manually annotated by BRENDA team
-
PLC-beta4 is present at the highest level in the cerebellum and retina
Manually annotated by BRENDA team
-
of seedlings
Manually annotated by BRENDA team
O24297
higher expression of PsPLC delta isoform in roots than in shoots
Manually annotated by BRENDA team
Q6SA76, Q6TM09, Q6TM10
the Vr-PLC3 protein is specifically activated by drough and salt stress in an abscisic acid-independent manner, with its induction being faster in roots than in leaf tissue
Manually annotated by BRENDA team
-
isoenzyme PI-PLC-1
Manually annotated by BRENDA team
O24297
light-grown
Manually annotated by BRENDA team
Q6QJ78
at three-leaf stage
Manually annotated by BRENDA team
-
containing hypocotyl and roots
Manually annotated by BRENDA team
-
the gamma1 isoenzyme is restricted to the sperm head
Manually annotated by BRENDA team
-
of seedlings
Manually annotated by BRENDA team
-
AtPLC1S is concentrated in shoot and leaf
Manually annotated by BRENDA team
Q39033
AtPLC2 expression
Manually annotated by BRENDA team
O24297
lower expression of PsPLC delta isoform in shoots than in roots
Manually annotated by BRENDA team
Q99P84
expression of PLCepsilon
Manually annotated by BRENDA team
Q9P212
only PLC epsilon1a, splice variant PLC epsilon1b can not be detected
Manually annotated by BRENDA team
-
PLC-delta4 mRNA is expressed in skeletal muscle. PLC-delta3 is present in skeletal muscle. PLC-delta1 is present in high abundancy in the brain, heart, lung, skeletal muscle and testis
Manually annotated by BRENDA team
Rattus norvegicus Sprague-Dawley, Mus musculus C57BL
-
-
-
Manually annotated by BRENDA team
Rattus norvegicus PLCepsilon
-
expression of PLCepsilon
-
Manually annotated by BRENDA team
Q99P84
expression of PLCepsilon
Manually annotated by BRENDA team
Rattus norvegicus PLCepsilon
-
expression of PLCepsilon
-
Manually annotated by BRENDA team
Q8K4D7, Q8R3B1
sperm-specific PLCzeta
Manually annotated by BRENDA team
-
PLC delta1, delta3 and delta4
Manually annotated by BRENDA team
Q9P212
PLC epsilon1b is enriched in
Manually annotated by BRENDA team
Q39032, Q39033, Q56W08, Q6NMA7, Q8GV43, Q944C1, Q944C2, Q9LY51, Q9STZ3
AtPLC1 is expressed at higher levels in stems than in the other organs
Manually annotated by BRENDA team
Q39032, Q39033, Q56W08, Q6NMA7, Q8GV43, Q944C1, Q944C2, Q9LY51, Q9STZ3
AtPLC7 is expressed at higher levels in stems than in the other organs
Manually annotated by BRENDA team
-
the elongating stem
Manually annotated by BRENDA team
-
the lower regions of the stem
Manually annotated by BRENDA team
Q8K4D7, Q8R3B1
-
Manually annotated by BRENDA team
-
PLC delta1, delta3 and delta4
Manually annotated by BRENDA team
Q8K4D7, Q8R3B1
PLCzeta
Manually annotated by BRENDA team
Q9P212
only PLC epsilon1a, splice variant PLC epsilon1b can not be detected
Manually annotated by BRENDA team
-
PLC-delta4 mRNA is expressed in testis. PLC-delta1 is present in high abundancy in the brain, heart, lung, skeletal muscle and testis
Manually annotated by BRENDA team
-
and connective tissue, PLC-delta1
Manually annotated by BRENDA team
Q9P212
only PLC epsilon1a, splice variant PLC epsilon1b can not be detected
Manually annotated by BRENDA team
Q9TZN8
lower TcPI-PLC expression than in epimastigotes and amastigotes
Manually annotated by BRENDA team
-
constitutive expression of AtPLC2 in vegetative and floral tissues
Manually annotated by BRENDA team
additional information
Q9NQ66
expression patterns of the two PLCbeta1 isoforms in a comprehensive panel of tissues
Manually annotated by BRENDA team
additional information
-
PLC delta2 is not expressed in muscles and digestive organs, nor in hematopoietic cells and lymphoid tissues
Manually annotated by BRENDA team
additional information
-
PLC-beta2 is highly expressed in cells of the immune system
Manually annotated by BRENDA team
additional information
O24297
the PsPLC delta isoform gene is expressed in all pea tissues, expression is regulated by light in a tissue specific manner
Manually annotated by BRENDA team
additional information
-
the two alternatively spliced forms of PLC-epsilon are present in a wide variety of human tissues
Manually annotated by BRENDA team
additional information
Q62711, Q99P84, Q9QW07
no activity astocytes
Manually annotated by BRENDA team
additional information
-
enzyme activity is assayed in tissue homogenates from gills and digestive gland
Manually annotated by BRENDA team
additional information
Q9P212
PLCepsilon1a can not be detected in SW260 cells and SW480 cells. PLCepsilon can not be detected in LCL cell line, Jurkat cell line, HeLa cell line, A431 cell line, and MCF7 cell line. Splice variants PLC epsilon1a and PLC epsilon1b can not be detected in the peripheral blood leukocyte
Manually annotated by BRENDA team
additional information
Q6SA76, Q6TM09, Q6TM10
Vr-PLC1 and Vr-PLC2 transcripts are constitutively expressed to varying degrees in every tissue of mung bean plants examined
Manually annotated by BRENDA team
additional information
Q6SA76, Q6TM09, Q6TM10
Vr-PLC3 mRNA level is very low under normal growth conditions and is rapidly induced in an abscisic acid-independent manner under environmental stress conditions (drought and high salinity). Using a green fluorescent protein (GFP) shows that the GFP-Vr-PLC3 fusion protein is localized primarily to the plasma membrane of the Arabidopsis protoplast
Manually annotated by BRENDA team
additional information
-
enzyme is not exclusively cytosolic
Manually annotated by BRENDA team
additional information
B2LSE7
L2 cell line and aluminum-tolerant LAMt cell line
Manually annotated by BRENDA team
additional information
Mytilus galloprovincialis Lam
-
enzyme activity is assayed in tissue homogenates from gills and digestive gland
-
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
P10687
PLCbeta1b targets to the sarcolemma and is enriched in caveolae
Manually annotated by BRENDA team
Q9NQ66
inner side, PLCbeta1
-
Manually annotated by BRENDA team
-
the PH domain of PLC-delta1 may tether the enzyme to the cell membrane by specific binding to phosphatidylinositol 4,5-bisphosphate
-
Manually annotated by BRENDA team
Rattus norvegicus PLC-delta1
-
the PH domain of PLC-delta1 may tether the enzyme to the cell membrane by specific binding to phosphatidylinositol 4,5-bisphosphate
-
-
Manually annotated by BRENDA team
Q62711, Q99P84, Q9QW07
-
Manually annotated by BRENDA team
-
phosphoinositidase C delta is restricted to the cytopasm
Manually annotated by BRENDA team
-
isoenzyme PIC gamma1, no activity of isoenzyme PIC beta1
Manually annotated by BRENDA team
Q9NQ66
PLCbeta1
Manually annotated by BRENDA team
Q9NQ66
in C2C12 cells PI-PLC beta3 is both in the nucleus and in the cytoplasm
Manually annotated by BRENDA team
-
PLCbeta1 is first exclusively localized to the nucleus and then migrates to the cytoplasm when the oocyte is fully grown. During oocyte maturation PLCbeta1 is active in the cytoplasm before it migrates and becomes active in the nucleus just prior to germinal vesicle breakdown
Manually annotated by BRENDA team
Q62711, Q99P84, Q9QW07
strong activity in cytoplasm of astrocytes, strong activity in cytoplasm of C6 cells
Manually annotated by BRENDA team
Q62711, Q99P84, Q9QW07
strong activity in cytoplasm of astrocytes, strong activity in cytoplasma of C6 cells, PI-PLC delta3
Manually annotated by BRENDA team
Q62711, Q99P84, Q9QW07
strong activity in cytoplasm of astrocytes, weak activity in cytoplasma of C6 cells
Manually annotated by BRENDA team
Q62711, Q99P84, Q9QW07
weak activity in cytoplasm of astrocytes, strong activity in cytoplasm of C6 cells
Manually annotated by BRENDA team
P10687
PLCbeta1a is localized in the cytoplasm
Manually annotated by BRENDA team
-
enzyme subtype beta2
Manually annotated by BRENDA team
-
majority of activity of isoenzyme beta1
Manually annotated by BRENDA team
Q9FSW1
DsPLC2 is most likely located in the cytosol
Manually annotated by BRENDA team
-
PTEN induces translocation of PLC-gamma from the cytosol to the membrane
Manually annotated by BRENDA team
-
PI-PLCm represents the main activity detected in the membrane, while PI-PLCc is the main activity present in the cytoplasm
Manually annotated by BRENDA team
-
PLC is a soluble protein that is localized mainly in the cytosol and is translocated to the plasma membrane, where it hydrolyzes PI(4,5)P2 in response to cell activation
Manually annotated by BRENDA team
Mus musculus C57BL/6
-
-
-
Manually annotated by BRENDA team
O24297
recombinant PLC delta isoform expressed in Escherichia coli
Manually annotated by BRENDA team
-
associated with
Manually annotated by BRENDA team
-
rat parotid gland membranes
Manually annotated by BRENDA team
-
enzyme subtype beta1 and beta3
Manually annotated by BRENDA team
-
PTEN induces translocation of PLC-gamma from the cytosol to the membrane
Manually annotated by BRENDA team
-
PI-PLCm represents the main activity detected in the membrane, while PI-PLCc is the main activity present in the cytoplasm
Manually annotated by BRENDA team
-
one of the isozymes is a membrane-associated PI-PLC
Manually annotated by BRENDA team
Rattus norvegicus Sprague-Dawley, Mus musculus C57BL
-
-
-
Manually annotated by BRENDA team
-
isoenzyme PIC beta1, and PIC gamma1
Manually annotated by BRENDA team
-
5% of the beta1-isoenzyme activity, no gamma isoenzyme or delta isoenzyme found
Manually annotated by BRENDA team
-
phosphoinositidase C beta is exclusively localized in the nucleus
Manually annotated by BRENDA team
-
all the four isoenzymes of the beta family are detectable in nuclei, beta1, beta2, beta3 and beta4, but not PLCgamma and PLCdelta, membrane deprived nuclei possess a higher amount of PLCs, beta isoenzymes are localized in the inner nuclear compartment and not in the nuclear envelope
Manually annotated by BRENDA team
-
PLC-beta1 is the major isoform of the nucleus
Manually annotated by BRENDA team
Q9NQ66
PLCbeta1
Manually annotated by BRENDA team
Q9Z1B3
PLCbeta1
Manually annotated by BRENDA team
-
an early increase in PLC-beta1b activity (6 h after partial hepatectomy) in nuclear matrix is associated with serine phosphorylation of the enzyme, the later increase (20 h after partial hepatectomy) paralleles the increase in the amount of protein
Manually annotated by BRENDA team
-
nuclear matrix, PLC-gamma1
Manually annotated by BRENDA team
-
in C2C12 cells PI-PLC beta1 is mainly located at the nucleus. During insulin-induced myoblast differentiation, PI-PLC beta1 is greatly increased at the nuclear level. The nuclear localization of PI-PLC beta1 is essential for induction of differentiation
Manually annotated by BRENDA team
Q9NQ66
in C2C12 cells PI-PLC beta3 is both in the nucleus and in the cytoplasm. During insulin-induced myoblast differentiation, expression of PI-PLC beta3 in C2C12 cells remains unchanged
Manually annotated by BRENDA team
-
PLCbeta1 is first exclusively localized to the nucleus and then migrates to the cytoplasm when the oocyte is fully grown. During oocyte maturation PLCbeta1 is active in the cytoplasm before it migrates and becomes active in the nucleus just prior to germinal vesicle breakdown
Manually annotated by BRENDA team
-
PLCdelta1 localizes in the nucleus during the g1/S boundary and the G0 phases of the cell cycle
Manually annotated by BRENDA team
-
two waves of nuclear PI-PLCbeta1b activity occur in serum-stimulated cells during G1 phase of the cell cycle
Manually annotated by BRENDA team
Q62711, Q99P84, Q9QW07
very weak activity in nucleus of astrocytes, very weak activity in nucleus of C6 cells
Manually annotated by BRENDA team
Q62711, Q99P84, Q9QW07
weak activity in nucleus of astrocytes, strong activity in nucleus of C6 cells
Manually annotated by BRENDA team
Q62711, Q99P84, Q9QW07
weak activity in nucleus of astrocytes, very weak activity in nucleus of C6 cells
Manually annotated by BRENDA team
Q62711, Q99P84, Q9QW07
weak activity in nucleus of astrocytes, very weak activity in nucleus of C6 cells, PI-PLC delta3
Manually annotated by BRENDA team
-
the basic cluster of phospholipase C-zeta could not only anchor the protein to the membrane, but also enhance the local concentration of phosphatidylinositol 4,5-bisphosphate adjacent to the catalytic domain
-
Manually annotated by BRENDA team
Q39033
predominantly localized in
Manually annotated by BRENDA team
-
enzyme activity is lower in plasma membranes isolated from light-grown shoots than from dark-grown ones
Manually annotated by BRENDA team
Q9TZN8
associated with, in amastigotes
Manually annotated by BRENDA team
-
activation of PLC by muscarinic acid or purinergic receptor stimulation results in translocation from the plasma membrane to the cytoplasm
Manually annotated by BRENDA team
Q3ZTU7
extracellular phase of plasma membrane
Manually annotated by BRENDA team
Q6SA76, Q6TM09, Q6TM10
the Vr-PLC3 protein is predominantly localized to plasma membrane
Manually annotated by BRENDA team
-
PLC is a soluble protein that is localized mainly in the cytosol and is translocated to the plasma membrane, where it hydrolyzes PI(4,5)P2 in response to cell activation. Membrane association of PLCdelta1 is mediated by its PH domain
Manually annotated by BRENDA team
Q9FSW1
DsPLC2, mesophyll cell protoplasts from leaves
-
Manually annotated by BRENDA team
P10687
PLCbeta1b targets to the sarcolemma and is enriched in caveolae
Manually annotated by BRENDA team
Rattus norvegicus Sprague-Dawley, Mus musculus C57BL
-
-
-
-
Manually annotated by BRENDA team
additional information
P10687
deletion of 3 amino acids from the C terminus of PLCbeta1b does not alter its sarcolemmal localization, but deletion of the entire unique 31 amino acid sequence causes cytosolic localization. PLCbeta1a expression is lost during postnatal development
-
Manually annotated by BRENDA team
additional information
-
PLC-gamma remains localized in the cytosolic compartment in the C124S-PTEN and G129E-PTEN cell lines where lipid-phosphatase-inactive PTEN is overexpressed
-
Manually annotated by BRENDA team
additional information
P19174
PLC-gamma1 is bound to phosphorylated nephrin Tyr-1204, which induces translocation of PLC-gamma1 from cytoplasm to the CD8/Nephrin cluster on the plasma membrane
-
Manually annotated by BRENDA team
additional information
Q6SA76, Q6TM09, Q6TM10
Vr-PLC1 and Vr-PLC2 transcripts are constitutively expressed to varying degrees in every tissue of mung bean plants examined
-
Manually annotated by BRENDA team
additional information
-
enzyme is predominantly localized in the soluble fraction
-
Manually annotated by BRENDA team
additional information
-
isozyme distribution, overview
-
Manually annotated by BRENDA team
PDB
SCOP
CATH
ORGANISM
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
18000
-
purified Pi-PLC (membrane) has a molecular weight of about 18000, SDS-PAGE or gel filtration
285237
34700
B2LSE7
sequence analysis
710328
40000 - 43000
-
enzyme form II, gel filtration
285216
57000
-
purified Pi-PLC (cytosol), SDS-PAGE or gel filtration
285237
58000 - 76000
-
enzyme form III, gel filtration
285216
58000
-
SDS-PAGE, low molecular weight PLC hydrolyzes both phosphatidylinositol and phosphatidylinositol 4,5-bisphosphate has a molecular mass of 58 kDa. Cloning techniques have tentatively identified the low molecular weight peak as PLC-alpha
135140
66000
-
low molecular PLC by size exclusion chromatography
135140
67400
Q6SA76, Q6TM09, Q6TM10
primary sequence of Vr-PLC3
668588
68000 - 70000
-
membrane-bound enzyme, in presence of sodium cholate, gel filtration
135123
71000 - 72000
-
cytosolic enzyme, in presence of sodium deoxycholate, gel filtration
135123
85000
-
Western blot analysis
694143
105000 - 120000
-
enzyme form I, gel filtration
285216
110000
-
gel filtration
135119
121000
Q3ZTU7
calculated from sequence
667524
135000
-
Western blot analysis
694143
140000
-
PLC-II
285234
140000
P10687
two splice variants, PLCbeta1a (150 kDa) and PLCbeta1b (140 kDa). Western Blot
692271
145000
-
high molecular PLC shows much greater activity against phosphatidylinositol than phosphatidylinositol 4,5-bisphosphate and is active at lower calcium concentrations
135140
150000
-
PLC-I
285234
150000
P10687
two splice variants, PLCbeta1a (150 kDa) and PLCbeta1b (140 kDa). Western Blot
692271
150000
-
Western blot analysis; Western blot analysis
694143
157000
-
high molecular PLC by size exclusion chromatography
135140
158500
-
gel filtration
135142
256000 - 271000
-
enzyme form IV, gel filtration
285216
additional information
-
PI-PLC, can be resolved into two peaks of activity of high M (60000-70000) and low M (16000-18000)
285237
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
?
-
x * 64000, SDS-PAGE
?
-
x * 100600, calculation from nucleotide sequence
?
-
x * 230000, x * 260000, two alternatively spliced PLC-epsilon forms
?
Q99P84
x * 255000, PLCepsilon, calculated from the amino acid sequence and Western blot analysis
?
O24297
x * 67440, PLC delta isoform, calculated from the amino acid sequence
?
Q9FSW1
x * 70800, DsPLC2, calculated from the amino acid sequence
?
Q8K4D7, Q8R3B1
x * 78000, recombinant His-tagged PLCzeta, SDS-PAGE
?
Q9TZN8
x * 82000, calculated from the amino acid sequence, x * 80000, SDS-PAGE, Western blot analysis
?
-
x * 85000, about, PLC-delta isoenzymes, x * 120000-155000, PLC-beta and -gamma isoenzymes, x * 230000-260000, PLC-epsilon
?
Q8K4D7, Q8R3B1
x * 90000, recombinant His-tagged PLCdelta1, SDS-PAGE
?
Q39033
x * 95000, recombinant GST-AtPLC2, SDS-PAGE, x * 66142, AtPLC2, calculated from the amino acid sequence
?
Q4KWH8
x * 115000, calculated from sequence
?
Q4KWH5
x * 115000, calculated from sequence
?
B2LSE7
x * 27000, SDS-PAGE or Western blot analysis
?
-
x * 44000, Western blot analysis
?
-
x * 44000, x * 32000, x * 30000, Western blot analysis
?
Rattus norvegicus PLCepsilon
-
x * 255000, PLCepsilon, calculated from the amino acid sequence and Western blot analysis
-
monomer
-
-
monomer
P34024
in solution in the absence of detergent
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
phosphoprotein
-
PLC-gamma1, phosphorylation sites: Tyr-771 and Tyr-783
phosphoprotein
-
PLCgamma2, phosphorylation in response to B-cell antigen receptor stimulation or H2O2 stress, phosphorylation of Tyr-753 and Tyr-759 activates enzyme
phosphoprotein
P16885
stimulation of PLCgamma2 activity by tyrosine phosphorylation, multiple phosphorylation sites, the kinase Btk phosphorylates recombinant PLCgamma2 in vitro, Tyr-753 and Tyr-759 are essential for PLCgamma2 function in B-cells, preferential phosphorylation of Tyr-753 over Tyr-759
phosphoprotein
-
phosphorylation of nuclear PLC-beta1b on serine occurs at the G2/M and the late G1 phase and is necessary for the progression of the cells through the cell cycle
phosphoprotein
-
PLC-gamma2 is phosphorylated on Y753, Y759, and Y1271 in response to engagement of the B-cell receptor in Ramos cells. In Ramos cells stimulated maximally with this receptor, the extent of phosphorylation of Y1217 is three times that of Y753 or of Y759. Stimulation of Jurkat cells or platelets via their immunoreceptors elicites phosphorylation of Y753 and Y759 but not that of Y1217. A basal level of phosphorylation of Y753 is apparent in unstimulated lymphocytes. The extent of phosphorylation of Y753 and Y759, but not that of Y1217 correlates with the lipase activity of PLC-gamma2. Btk is largely, but not completely, responsible for phosphorylation of Y753 and Y759, whereas phosphorylation of Y1217 is independent of Btk. Phosphorylation of Y1217 and that of Y753 and Y759 occurs on different PLC-gamma2 molecules
phosphoprotein
-
PLC-gamma1 and 2, tyrosine phosphorylation activates enzyme
phosphoprotein
-
increased phosphorylation of nuclear PLCbeta1, due to nuclear translocation of MAP kinase, is required for the activation of the nuclear inositol lipid cycle in 3T3 cells treated with insulin-like growth factor 1
phosphoprotein
-
PLC-gamma2 is phosphorylated on Y753, Y759, and Y1271 in response to engagement of the B-cell receptor in spleenic cells. In spleenic cells stimulated maximally with this receptor, the extent of phosphorylation of Y1217 is three times that of Y753 or of Y759
phosphoprotein
-
an early increase in PLC-beta1b activity (6 h after partial hepatectomy) in nuclear matrix is associated with serine phosphorylation of the enzyme, the increase in the PLC-gamma1 activity measured at 6 h and 20 h after partial hepatectomy is associated with tyrosine phosphorylation of the enzyme
side-chain modification
-
rat brain protein kinase C phosphorylates phosphoinositidase gamma but not phosphoinositidase beta1
lipoprotein
Q9TZN8
TcPI-PLC is lipid modified in vivo, TcPI-PLC contains an N-myristoylation consensus sequence at its N-terminal end and is modified by myristate and palmitate, myristoyl group may play an important role in the association of enzyme with the plasma membrane
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
free form, in complex with myo-inositol and with glucosaminyl(alpha1-6)-D-myo-inositol
-
hanging drop vapor diffusion method, X-ray crystal structure of R69D at 2.1 A resolution
-
2.4 A structure of phospholipase Cdelta1
-
of phosphatidylinositide-specific phospholipase deltaDELTA1-134
-
PLC-delta1 complexed with inositol 1,4,5-trisphosphate
-
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
-
both PI-PLC (membrane) and PI-PLC (cytosol) are fairly stable proteins when incubated for short periods of time (30 min) to a wide range of pH. PI-PLC (membrane) shows a fall of about 20-30% activity below pH 5.0 and is stable to alkaline pH up to 11
285237
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
Partially purified PLC can be stored in buffer C at -80C for several months without appreciable loss of activity. However, repeated freezing and thawing has a deleterious effect on enzyme activity. More purified PLC (Mono Q and size exclusion fractions) can be stored at 4C for 5-7 days with a gradual loss of activity
-
-20C, stable for several month
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant AtPLC2
Q39033
near homogeneity
-
PLC delta2, from cerebral cortex
-
PLC-beta1
-
by Ni-NTA chromatography
B2LSE7
recombinant DsPLC2
Q9FSW1
partial, 56.5fold
-
confirmed by Coomassie blue staining after SDS-PAGE
-
enzyme from platelet membrane
-
preparation of nuclei, method development
Q9NQ66
recombinant PLCgamma2, expressed in Sf9 cells
P16885
chitin column and SDS-PAGE
P34024
recombinant
-
recombinant
Q7T274
enzyme PLC-delta1 prepared by a baculovirus expression system; enzyme prepared by a baculovirus expression system; recombinant PLCdelta1; recombinant PLCzeta
Q8K4D7, Q8R3B1
partial
-
preparation of nuclei, method development
Q9Z1B3
from inclusion bodies, inactive recombinant PLC delta isoform, may be due to an incorrect refolding
O24297
enzyme form beta3; enzyme subtype beta1 and beta 3 from brain, enzyme subtype beta2 from HL60 cells
-
partially purified, DEAE-cellulose and heparin-Sepharose chromatographies followed by Mono Q and size exclusion HPLC
-
preparation of nuclei, method development
P10687
recombinant PLCepsilon
Q99P84
recombinant His-tagged PI-PLC1 from Escherichia coli strain BL21 by nickel affinity chromatography
-
recombinant TcPI-PLC
Q9TZN8
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
AtPLC2, full-length and deletion variants, expression in Escherichia coli BL21(DE3)pLys
Q39033
nine AtPLC genes, they are induced in response to various environmental stimuli, including cold, salt, nutrients Murashige-Skoog salts, dehydration, and the plant hormone abscisic acid; nine AtPLC genes, they are induced in response to various environmental stimuli, including cold, salt, nutrients Murashige-Skoog salts, dehydration, and the plant hormone abscisic acid; nine AtPLC genes, they are induced in response to various environmental stimuli, including cold, salt, nutrients Murashige-Skoog salts, dehydration, and the plant hormone abscisic acid; nine AtPLC genes, they are induced in response to various environmental stimuli, including cold, salt, nutrients Murashige-Skoog salts, dehydration, and the plant hormone abscisic acid; nine AtPLC genes, they are induced in response to various environmental stimuli, including cold, salt, nutrients Murashige-Skoog salts, dehydration, and the plant hormone abscisic acid; nine AtPLC genes, they are induced in response to various environmental stimuli, including cold, salt, nutrients Murashige-Skoog salts, dehydration, and the plant hormone abscisic acid; nine AtPLC genes, they are induced in response to various environmental stimuli, including cold, salt, nutrients Murashige-Skoog salts, dehydration, and the plant hormone abscisic acid
Q39032, Q39033, Q56W08, Q6NMA7, Q8GV43, Q944C1, Q944C2, Q9LY51, Q9STZ3
the first 108 amino acid residues of the N-terminal of AtPLC4, referred to as AtPLC4 N, is expressed as a recombinant protein in Escherichia coli and used as antigen in generating antibody
Q944C1
expressed in Escherichia coli BL21-Codonplus (DE3)-RIL cell
-
into the pET11d vector, expressed as a histidine-tagged protein in Escherichia coli BL21 cells
B2LSE7
expression of sc1 in Saccharomyces cerevisiae DELTAisc1 mutant
-
full-length DsPLC2 cDNA, expression of two proteins synthesized from two potential initiation codons in Escherichia coli BL21, sequencing
Q9FSW1
-
Q4KWH8
expressed cDNA constructs encoding PLC-beta2 fragments of different lengths in COS-7 cell. Separate expression of amino- and C-terminal fragments of enzyme, requirements for reconstitution of enzyme activity and activation by Gbetagamma
-
expression in baculovirus-infected insect cells
-
expression in COS or HEK293 cells
Q9P212
expression in COS-7 cells
P51178
expression in Escherichia coli
-
two distinct PLCbeta1 cDNAs, PLCbeta1a and b, generated through alternative splicing at their 3 end, sequencing, overexpression in CHO and PC12 cells, gene structure, located on chromosome 20
Q9NQ66
wild-type and mutant PLCgamma2, expression in DT40 B-cells
-
wild-type and mutant PLCgamma2, expression in DT40 cells, A431 cells and in Sf9 cells
P16885
expression in Escherichia coli; expression in Escherichia coli
Q2YGU0, Q2YGU1
transformed into Escherichia coli ER2566 cells for overexpression
P34024
expression in Escherichia coli BL21 (DE3) using pET28a vector, expression as a histidine-tagged fusion protein
Q7T274
microinjection of recombinant PLCzeta protein into mouse eggs induces serial Ca2+ spikes quite similar to that produced by the injection of sperm extract, probably because of repetitive Ca2+ release from the endoplasmic reticulum caused by continuously produced inositol 1,4,5-trisphosphate; PLC-delta1; PLCdelta1, expression in Sf9 cells; PLCzeta, expression in Sf9 cells
Q8K4D7, Q8R3B1
PLC delta isoform, expression in Escherichia coli BRL (DE3), sequencing, genomic organization
O24297
enzyme expression in Escherichia coli strain BL21 (DE3)
-
expression in COS-7 cells
-
PLCepsilon, expression in Sf-9 cells and in COS-7 cells
Q99P84
transfection of C2C12 cells with expression vectors containing PLC beta1 and with small interfering RNAs from regions of the PLC beta1 gene
-
full-length PLC6 cDNA amplified, in-frame cloning of PLC6 into the pGEX-KG plasmid resulting in a GST-PLC6 fusion. For the GST-PLC4, PLC4 amplified from a plasmid containing full-length PLC4, cloned into pGEMT and then isolated by digestion with XbaI and XhoI. Both expressed in Escherichia coli strain BL21. PLC4 expression construct ligated into the XhoI and EcoRI restriction sites downstream of the 35S promoter of a pMOG800-based binary vector Nicotiana benthamiana
-
expression in Escherichia coli BL21
Q3ZTU7
expression of His-tagged PI-PLC1 in Escherichia coli strain BL21. Pi-Plc1 and Pi-Plc2, expression of full-length enzymes in Nicotiana benthamiana via Agrobacterium tunefaciens strain AGL1 transfection method, co-expression with GA3 and Clo3
-
expression in Escherichia coli BL21(DE3), sequencing
Q9TZN8
the GFP-Vr-PLC3 and control smGFP plasmids are introduced into protoplasts prepared from Arabidopsis seedlings by polyethylene glycol treatment
Q6SA76, Q6TM09, Q6TM10
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
PLC5 transcripts show the lowest abundance in each organ. Expression of PLC5 is repressed in mock-treated plants. In incompatible interaction, increase is transient for PLC1 and PLC4, as their expression levels decrease again at day 10 to reach the same levels as in the mock-treated plants
-
in mock-treated plants, PLC2, PLC3, PLC4 and PLC6 expression levels are relatively stable. In incompatible interaction, the expression levels of PLC3 and PLC6 are not significantly affected
-
PLC3 is the most abundantly expressed PLC gene. Expression of PLC1 is induced in mock-treated plants. In incompatible interaction, levels of PLC1, PLC2, PLC4 and PLC5 transcripts significantly increase
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
G48A
-
single PI-PLC mutant, site-directed mutagenesis
I43A
-
single PI-PLC mutant, site-directed mutagenesis
I43W
-
single PI-PLC mutant, site-directed mutagenesis
K44E
-
single PI-PLC mutant, site-directed mutagenesis
L39A
-
single PI-PLC mutant, site-directed mutagenesis
P42G
-
single PI-PLC mutant, site-directed mutagenesis
Q45A
-
single PI-PLC mutant, site-directed mutagenesis
V46A
-
single PI-PLC mutant, site-directed mutagenesis
D114A
-
catalytically inactive Isc1 mutant
D114A
Cryptococcus neoformans H99
-
catalytically inactive Isc1 mutant
-
R257H
-
increased expression of mutant PLC delta1 in patients with coronary spastic angina
R564A/R672A
-
PLCgamma2 SH2 domain double mutant
T753F
P16885
PLCgamma2 mutant
T753F/T759F
P16885
active PLCgamma2 double mutant with a loss of tyrosine phosphorylation
T753F/T759F