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Literature summary for 3.1.4.11 extracted from

  • Chen, W.; Goldfine, H.; Ananthanarayanan, B.; Cho, W.; Roberts, M.
    Listeria monocytogenes phosphatidylinositol-specific phospholipase C: Kinetic activation and homing in on different interfaces (2009), Biochemistry, 48, 3578-3592.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.4.11

Activating Compound

Activating Compound Comment Organism Structure
inositol 1,2-(cyclic)-phosphate
-
 Listeria monocytogenes
additional information activation by amphiphiles occurs with both micellar (phosphatidylinositol dispersed in detergents) and monomeric [dibutroylphosphatidylinositol (diC4PI)] phosphotransferase substrates and inositol 1,2-(cyclic)-phosphate (cIP), the phosphodiesterase substrate Listeria monocytogenes

Cloned(Commentary)

Cloned (Comment) Organism
transformed into Escherichia coli ER2566 cells for overexpression Listeria monocytogenes

Protein Variants

Protein Variants Comment Organism
F237A site directed mutagenesis Listeria monocytogenes
F237W point mutation of surface hydrophobic residues Listeria monocytogenes
L151A site directed mutagenesis Listeria monocytogenes
L235A point mutation of surface hydrophobic residues Listeria monocytogenes
L51A point mutation of surface hydrophobic residues Listeria monocytogenes
W49A point mutation of surface hydrophobic residues Listeria monocytogenes
W49A/F237A site directed mutagenesis Listeria monocytogenes

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
12.5
-
 phosphatidylinositol at the lower PI-PLC concentration (3.6 microg/ml) Listeria monocytogenes
100
-
 phosphatidylinositol at double the enzyme concentration Listeria monocytogenes

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Listeria monocytogenes
-
-

Organism

Organism UniProt Comment Textmining
Listeria monocytogenes A0A0H3GCV8
-
-

Purification (Commentary)

Purification (Comment) Organism
chitin column and SDS-PAGE Listeria monocytogenes

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 measured using 31P NMR spectroscopy, loss of activity with high enzyme concentrations. Specific activity of the enzyme can be enhanced by dilution of the protein or by addition of both zwitterionic and neutral amphiphiles (e.g., diheptanoylphosphatidylcholine or Triton X-100) or 0.1-0.2 M inorganic salts Listeria monocytogenes
0.7
-
 dibutroylphosphatidylinositol as substrate Listeria monocytogenes
1.2
-
 dibutroylphosphatidylinositol as substrate, dihexanoylphosphatidylcholine as amphiphile Listeria monocytogenes
1.2
-
 dibutroylphosphatidylinositol as substrate, KCl 0.075 M Listeria monocytogenes
1.3
-
 phosphatidylinositol as substrate Listeria monocytogenes
1.8
-
 dibutroylphosphatidylinositol as substrate, Triton X-100 as amphiphile Listeria monocytogenes
9
-
 phosphatidylinositol as substrate, 1-palmitoyl-2-oleoylphosphatidylcholine as amphiphile Listeria monocytogenes
10
-
 dibutroylphosphatidylinositol as substrate, dihexanoylphosphatidylcholine as amphiphile, KCl 0.075 M Listeria monocytogenes
11.2
-
 dibutroylphosphatidylinositol as substrate, Triton X-100 as amphiphile, KCl 0.075 M Listeria monocytogenes
32.7
-
 1.6 microg/ml PI-PLC, citraconic anhydride modification 2 (chemical modification with citraconic anhydride changes the positive charges of lysine residues on the protein surface to negative ones) Listeria monocytogenes
54
-
 phosphatidylinositol as substrate, KCl 0.10 M Listeria monocytogenes
60.6
-
 1.6 microg/ml PI-PLC, control Listeria monocytogenes
82.5
-
 1.6 microg/ml PI-PLC, citraconic anhydride modification 1 (chemical modification with citraconic anhydride changes the positive charges of lysine residues on the protein surface to negative ones) Listeria monocytogenes
109
-
 0.2 microg/ml PI-PLC, citraconic anhydride modification 2 (chemical modification with citraconic anhydride changes the positive charges of lysine residues on the protein surface to negative ones) Listeria monocytogenes
127
-
 phosphatidylinositol as substrate, 1-palmitoyl-2-oleoylphosphatidylcholine as amphiphile, (NH4)2SO4 0.10 M Listeria monocytogenes
165
-
 0.2 microg/ml PI-PLC, citraconic anhydride modification 1 (chemical modification with citraconic anhydride changes the positive charges of lysine residues on the protein surface to negative ones) Listeria monocytogenes
274
-
 phosphatidylinositol as substrate, Triton X-100 as amphiphile Listeria monocytogenes
303
-
 0.2 microg/ml PI-PLC, control Listeria monocytogenes
330
-
 phosphatidylinositol as substrate, KCl 0.20 M Listeria monocytogenes
403
-
 0.2 microg/ml PI-PLC, 0.15 M KCl, citraconic anhydride modification 2 (chemical modification with citraconic anhydride changes the positive charges of lysine residues on the protein surface to negative ones) Listeria monocytogenes
511
-
 phosphatidylinositol as substrate, KCl 0.30 M Listeria monocytogenes
534
-
 0.2 microg/ml PI-PLC, citraconic anhydride modification 2 (chemical modification with citraconic anhydride changes the positive charges of lysine residues on the protein surface to negative ones) Listeria monocytogenes
936
-
 phosphatidylinositol as substrate, Triton X-100 as amphiphile, NH4Cl 0.15 M Listeria monocytogenes
990
-
 0.2 microg/ml PI-PLC, 0.15 M KCl, citraconic anhydride modification 1 (chemical modification with citraconic anhydride changes the positive charges of lysine residues on the protein surface to negative ones) Listeria monocytogenes
1030
-
 0.2 microg/ml PI-PLC, 0.15 M KCl, control Listeria monocytogenes
1030
-
 phosphatidylinositol as substrate, Triton X-100 as amphiphile, KCl 0.15 M Listeria monocytogenes
1120
-
 phosphatidylinositol as substrate, Triton X-100 as amphiphile, K2SO4 0.10 M Listeria monocytogenes
1150
-
 0.2 microg/ml PI-PLC, control Listeria monocytogenes
1430
-
 phosphatidylinositol as substrate, Triton X-100 as amphiphile, (NH4)2SO4 0.10 M Listeria monocytogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dibutyrylphosphatidylinositol + H2O
-
 Listeria monocytogenes ?
-
 ?
additional information water-soluble synthetic short-chain phosphatidylinositol and inositol 1,2-(cyclic)-phosphate along with PI/detergent mixed micelles and PI/PC vesicles are used as substrates Listeria monocytogenes ?
-
 ?
phosphatidylinositol + H2O
-
 Listeria monocytogenes diacylglycerol + inositol 1,2-(cyclic)-phosphate
-
 ?

Subunits

Subunits Comment Organism
monomer in solution in the absence of detergent Listeria monocytogenes

Synonyms

Synonyms Comment Organism
cyclic phosphodiesterase
-
 Listeria monocytogenes
phosphatidylinositol-specific phospholipase
-
 Listeria monocytogenes
phosphodiesterase
-
 Listeria monocytogenes
PI-PLC
-
 Listeria monocytogenes
plcA
-
 Listeria monocytogenes

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Listeria monocytogenes