EC Number   |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
|---|
   3.1.3.48 | 0.00016 |
- |
6,8-difluoro-4-methylumbelliferyl phosphate |
MKP-4, in 20 mM Tris-HCl (pH 8.0) containing 2 mM dithiothreitol |
681387 |
| 3.1.3.48 | 0.0035 |
- |
6,8-difluoro-4-methylumbelliferyl phosphate |
MKP-3, in 20 mM Tris-HCl (pH 8.0) containing 2 mM dithiothreitol |
681387 |
| 3.1.3.48 | 0.01 |
- |
4-nitrophenyl phosphate |
mutant enzyme D236A/Q314A, at 30°C, in 0.15 M Bis-Tris, pH 6.0 |
690919 |
| 3.1.3.48 | 0.0143 |
- |
6,8-difluoro-4-methylumbelliferyl phosphate |
pH 5.0, 20°C, mutant D63N |
751975 |
| 3.1.3.48 | 0.016 |
- |
KRLIEDNE(pY)TARGQ |
pH 8.0, presence of 1 mM Mn2+ |
665386 |
| 3.1.3.48 | 0.022 |
- |
4-nitrophenyl phosphate |
pH 8.0, presence of 1 mM Mn2+ |
665386 |
| 3.1.3.48 | 0.027 |
- |
4-nitrophenyl phosphate |
pH 7.3, 22°C, recombinant enzyme |
749850 |
| 3.1.3.48 | 0.03 |
- |
4-nitrophenyl phosphate |
mutant enzyme D236A, at 30°C, in 0.15 M Bis-Tris, pH 6.0 |
690919 |
| 3.1.3.48 | 0.033 |
- |
bis(4-nitrophenyl) phosphate |
pH 7.3, 22°C, recombinant enzyme |
749850 |
| 3.1.3.48 | 0.0371 |
- |
6,8-difluoro-4-methylumbelliferyl phosphate |
pH 5.0, 20°C, mutant R124E |
751975 |
