Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 (DE3) cells | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapour diffusion method with 0.05 M Bis-Tris (pH 7.0), 24% (v/v) PEG3350, 0.1 M lithium sulfate, 0.2 M magnesium sulfate and 10 mM dithiothreitol | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0111 | - |
6,8-difluoro-4-methylumbelliferyl phosphate | MKP-4, in 20 mM Tris-HCl (pH 8.0) containing 2 mM dithiothreitol | Homo sapiens | |
0.0173 | - |
6,8-difluoro-4-methylumbelliferyl phosphate | MKP-3, in 20 mM Tris-HCl (pH 8.0) containing 2 mM dithiothreitol | Homo sapiens | |
0.0448 | - |
6,8-difluoro-4-methylumbelliferyl phosphate | VHR, in 20 mM Tris-HCl (pH 8.0) containing 2 mM dithiothreitol | Homo sapiens | |
0.0795 | - |
6,8-difluoro-4-methylumbelliferyl phosphate | MKP-5, in 20 mM Tris-HCl (pH 8.0) containing 2 mM dithiothreitol | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q16828 | MKP-3 | - |
Homo sapiens | Q99956 | MKP-4 | - |
Homo sapiens | Q9Y6W6 | MKP-5 | - |
Purification (Comment) | Organism |
---|---|
nickel-affinity chromatography, Q-Sepharose chromatography and gel filtration | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6,8-difluoro-4-methylumbelliferyl phosphate + H2O | - |
Homo sapiens | 6,8-difluoro-4-methylumbelliferone + phosphate | - |
? | |
p38 + H2O | - |
Homo sapiens | ? | - |
? | |
phosphorylated JNK + H2O | - |
Homo sapiens | JNK + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MAP kinase phosphatase | - |
Homo sapiens |
mitogen-activated protein kinase phosphatase | - |
Homo sapiens |
MKP-3 | - |
Homo sapiens |
MKP-4 | - |
Homo sapiens |
MKP-5 | - |
Homo sapiens |
MKP5-C | catalytic domain of MKP5 | Homo sapiens |
vaccinia H1-related PTP | - |
Homo sapiens |
VHR | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00016 | - |
6,8-difluoro-4-methylumbelliferyl phosphate | MKP-4, in 20 mM Tris-HCl (pH 8.0) containing 2 mM dithiothreitol | Homo sapiens | |
0.0035 | - |
6,8-difluoro-4-methylumbelliferyl phosphate | MKP-3, in 20 mM Tris-HCl (pH 8.0) containing 2 mM dithiothreitol | Homo sapiens | |
0.15 | - |
6,8-difluoro-4-methylumbelliferyl phosphate | MKP-5, in 20 mM Tris-HCl (pH 8.0) containing 2 mM dithiothreitol | Homo sapiens | |
0.21 | - |
6,8-difluoro-4-methylumbelliferyl phosphate | VHR, in 20 mM Tris-HCl (pH 8.0) containing 2 mM dithiothreitol | Homo sapiens |