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Literature summary for 3.1.3.48 extracted from

  • Jeong, D.G.; Yoon, T.S.; Kim, J.H.; Shim, M.Y.; Jung, S.K.; Son, J.H.; Ryu, S.E.; Kim, S.J.
    Crystal structure of the catalytic domain of human MAP kinase phosphatase 5: structural insight into constitutively active phosphatase (2006), J. Mol. Biol., 360, 946-955.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapour diffusion method with 0.05 M Bis-Tris (pH 7.0), 24% (v/v) PEG3350, 0.1 M lithium sulfate, 0.2 M magnesium sulfate and 10 mM dithiothreitol Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0111
-
6,8-difluoro-4-methylumbelliferyl phosphate MKP-4, in 20 mM Tris-HCl (pH 8.0) containing 2 mM dithiothreitol Homo sapiens
0.0173
-
6,8-difluoro-4-methylumbelliferyl phosphate MKP-3, in 20 mM Tris-HCl (pH 8.0) containing 2 mM dithiothreitol Homo sapiens
0.0448
-
6,8-difluoro-4-methylumbelliferyl phosphate VHR, in 20 mM Tris-HCl (pH 8.0) containing 2 mM dithiothreitol Homo sapiens
0.0795
-
6,8-difluoro-4-methylumbelliferyl phosphate MKP-5, in 20 mM Tris-HCl (pH 8.0) containing 2 mM dithiothreitol Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q16828 MKP-3
-
Homo sapiens Q99956 MKP-4
-
Homo sapiens Q9Y6W6 MKP-5
-

Purification (Commentary)

Purification (Comment) Organism
nickel-affinity chromatography, Q-Sepharose chromatography and gel filtration Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6,8-difluoro-4-methylumbelliferyl phosphate + H2O
-
Homo sapiens 6,8-difluoro-4-methylumbelliferone + phosphate
-
?
p38 + H2O
-
Homo sapiens ?
-
?
phosphorylated JNK + H2O
-
Homo sapiens JNK + phosphate
-
?

Synonyms

Synonyms Comment Organism
MAP kinase phosphatase
-
Homo sapiens
mitogen-activated protein kinase phosphatase
-
Homo sapiens
MKP-3
-
Homo sapiens
MKP-4
-
Homo sapiens
MKP-5
-
Homo sapiens
MKP5-C catalytic domain of MKP5 Homo sapiens
vaccinia H1-related PTP
-
Homo sapiens
VHR
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00016
-
6,8-difluoro-4-methylumbelliferyl phosphate MKP-4, in 20 mM Tris-HCl (pH 8.0) containing 2 mM dithiothreitol Homo sapiens
0.0035
-
6,8-difluoro-4-methylumbelliferyl phosphate MKP-3, in 20 mM Tris-HCl (pH 8.0) containing 2 mM dithiothreitol Homo sapiens
0.15
-
6,8-difluoro-4-methylumbelliferyl phosphate MKP-5, in 20 mM Tris-HCl (pH 8.0) containing 2 mM dithiothreitol Homo sapiens
0.21
-
6,8-difluoro-4-methylumbelliferyl phosphate VHR, in 20 mM Tris-HCl (pH 8.0) containing 2 mM dithiothreitol Homo sapiens