Activating Compound | Comment | Organism | Structure |
---|---|---|---|
NaCl | activating up to 0.5 M | Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
D194A | compared to wild-type, 5.4% activity with 4-nitrophenyl phosphate | Bacillus subtilis |
D3A | compared to wild-type, 8% activity with 4-nitrophenyl phosphate | Bacillus subtilis |
H136A | compared to wild-type, 7.7% activity with 4-nitrophenyl phosphate | Bacillus subtilis |
H196A | compared to wild-type, 4.1% activity with 4-nitrophenyl phosphate | Bacillus subtilis |
H42A | compared to wild-type, 6.8% activity with 4-nitrophenyl phosphate | Bacillus subtilis |
H5A | compared to wild-type, 9.5% activity with 4-nitrophenyl phosphate | Bacillus subtilis |
H7A | compared to wild-type, 6.3% activity with 4-nitrophenyl phosphate | Bacillus subtilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diphosphate | 1 mM, 31% residual activity | Bacillus subtilis | |
NaF | 1 mM, 85% residual activity | Bacillus subtilis | |
vanadate | competitive, 1 mM, 11% residual activity | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.054 | - |
KRLIEDNE(pY)TARGQ | pH 8.0, presence of 1 mM Mn2+ | Bacillus subtilis | |
0.345 | - |
4-nitrophenyl phosphate | pH 8.0, presence of 1 mM Mn2+ | Bacillus subtilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | or Mn2+, Zn2+, required | Bacillus subtilis | |
Mn2+ | or Cu2+, Zn2+, required, Km-value 0.274 mM | Bacillus subtilis | |
additional information | not activating: Mg2+, Ca2+ | Bacillus subtilis | |
Zn2+ | or Cu2+, Mn2+, required | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate + H2O | - |
Bacillus subtilis | 4-nitrophenol + phosphate | - |
? | |
KRLIEDNE(pY)TARGQ + H2O | phosphopeptide derived from autophosphorylation of mammalian tyrosine kinase Lck | Bacillus subtilis | KRLIEDNEYTARGQ + phosphate | - |
? | |
additional information | no substrate: phosphoeptides RRA(pS)VA, KR(pT)IRR | Bacillus subtilis | ? | - |
? | |
phosphorylated YwqD | protein implicated in UDP-glucuronate synthesis | Bacillus subtilis | YwqD + phosphate | - |
? | |
phosphorylated YwqF | protein implicated in UDP-glucuronate synthesis | Bacillus subtilis | YwqF + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
YwqE | - |
Bacillus subtilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.016 | - |
KRLIEDNE(pY)TARGQ | pH 8.0, presence of 1 mM Mn2+ | Bacillus subtilis | |
0.022 | - |
4-nitrophenyl phosphate | pH 8.0, presence of 1 mM Mn2+ | Bacillus subtilis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9 | in presence of Mn2+, Zn2+, activity increases with pH-value. In presence of Cu2+, activity is realtively pH-independent | Bacillus subtilis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.014 | - |
vanadate | pH 8.0 | Bacillus subtilis |