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Literature summary for 3.1.3.48 extracted from

  • Critton, D.A.; Tortajada, A.; Stetson, G.; Peti, W.; Page, R.
    Structural basis of substrate recognition by hematopoietic tyrosine phosphatase (2008), Biochemistry, 47, 13336-13345.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21-CodonPlus (DE3) cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with monophosphorylated and dually phosphorylated Erk2 peptides, sitting drop vapour diffusion method, in 0.2 M ammonium tartrate, pH 6.6, and 20% (w/v) PEG 3350 or and 1.0 M lithium chloride, 0.1 M citrate, pH 5.0, and 20% (w/v) PEG 6000 Homo sapiens

Protein Variants

Protein Variants Comment Organism
C270A inactive, substrate-trapping mutant of the HePTP catalytic domain (HePTP residues 44-339) Homo sapiens
C270S inactive, substrate-trapping mutant of the HePTP catalytic domain (HePTP residues 44-339) Homo sapiens
C270S/T106D inactive, substrate-trapping mutant of the HePTP catalytic domain (HePTP residues 44-339) Homo sapiens
D236A substrate-trapping mutant of the HePTP catalytic domain (HePTP residues 44-339), shows 420fold reduced activity compared to the wild type enzyme Homo sapiens
D236A/C270A/Q314A inactive, substrate-trapping mutant of the HePTP catalytic domain (HePTP residues 44-339) Homo sapiens
D236A/C270S/Q314A inactive, substrate-trapping mutant of the HePTP catalytic domain (HePTP residues 44-339) Homo sapiens
D236A/Q314A substrate-trapping mutant of the HePTP catalytic domain (HePTP residues 44-339), shows 1255fold reduced activity compared to the wild type enzyme Homo sapiens
Q314A substrate-trapping mutant of the HePTP catalytic domain (HePTP residues 44-339), shows 13fold reduced activity compared to the wild type enzyme Homo sapiens
T106D KIM-PTP specific substrate-trapping mutant, the introduced aspartate side chain facilitates the coordination of the bound peptides, thereby stabilizing the active dephosphorylation complex Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.56
-
4-nitrophenyl phosphate mutant enzyme D236A/Q314A, at 30°C, in 0.15 M Bis-Tris, pH 6.0 Homo sapiens
1.31
-
4-nitrophenyl phosphate mutant enzyme D236A, at 30°C, in 0.15 M Bis-Tris, pH 6.0 Homo sapiens
2.11
-
4-nitrophenyl phosphate mutant enzyme Q314A, at 30°C, in 0.15 M Bis-Tris, pH 6.0 Homo sapiens
3.26
-
4-nitrophenyl phosphate wild type enzyme, at 30°C, in 0.15 M Bis-Tris, pH 6.0 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P35236
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap HP column chromatography and Superdex 75 gel filtration Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
hematopoietic cell
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
Homo sapiens 4-nitrophenol + phosphate
-
?
LDEpYVATR + H2O
-
Homo sapiens LDEYVATR + phosphate
-
?
phosphorylated ERK1 + H2O
-
Homo sapiens ERK1 + phosphate
-
?
phosphorylated ERK2 + H2O HePTP binds the phosphorylated tyrosine of the Erk2 peptide (pY185), Erk2 residue T183, which is phosphorylated in maximally activated Erk2, is not essential for substrate recognition and binding by HePTP Homo sapiens ERK2 + phosphate
-
?
phosphorylated p38 mitogen-activated protein kinase + H2O
-
Homo sapiens p38 mitogen-activated protein kinase + phosphate
-
?

Synonyms

Synonyms Comment Organism
hematopoietic tyrosine phosphatase
-
Homo sapiens
HEPTP HePTP negatively regulates T cell activation and proliferation Homo sapiens
KIM-PTP
-
Homo sapiens
kinase interaction motif phosphatase
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.01
-
4-nitrophenyl phosphate mutant enzyme D236A/Q314A, at 30°C, in 0.15 M Bis-Tris, pH 6.0 Homo sapiens
0.03
-
4-nitrophenyl phosphate mutant enzyme D236A, at 30°C, in 0.15 M Bis-Tris, pH 6.0 Homo sapiens
0.93
-
4-nitrophenyl phosphate mutant enzyme Q314A, at 30°C, in 0.15 M Bis-Tris, pH 6.0 Homo sapiens
12.55
-
4-nitrophenyl phosphate wild type enzyme, at 30°C, in 0.15 M Bis-Tris, pH 6.0 Homo sapiens