EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Reference |
---|
3.5.1.1 | -999 |
- |
- |
208957, 208960 |
3.5.1.1 | -999 |
- |
L-asparaginase activity depends on buffers and ions and ranges from 386 I.U/mg in 50 mM sodium phosphate buffer pH 6.0, 375 mM NaCl up to 510 I.U/mg in 50 mM Tris-HCl pH 7.5, 500 mM NaCl |
754682 |
3.5.1.1 | -999 |
- |
no glutaminase activity is observed |
710917 |
3.5.1.1 | -999 |
- |
the production and productivity of L-asparaginase is enhanced by 26.39% (specific activity) and 10.19%, respectively. The individual optimum levels of initial pH of the medium, temperature, rpm of shaking incubator, and inoculum size are found to be 6.90, 29.8°C, 157 rpm, and 2.61% (v/v), respectively, for the production of L-asparaginase. |
710917 |
3.5.1.1 | 0.27 |
- |
enzyme form C |
208955 |
3.5.1.1 | 0.7797 |
- |
crude enzyme, at 45°C in 50 mM phosphate buffer (pH 7.0) |
733171 |
3.5.1.1 | 0.82 |
- |
- |
684515 |
3.5.1.1 | 0.88 |
- |
crude cell extract, at pH 8.6 and 37°C |
734465 |
3.5.1.1 | 0.925 |
- |
with D-glutamine as substrate, at pH 8.5 and 85°C |
734492 |
3.5.1.1 | 1.41 |
- |
enzyme form A |
208955 |