EC Number   |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
|---|
    3.1.8.1 | 0.0000983 |
- |
demeton-S |
at 35°C, pH 7.2 |
707276 |
| 3.1.8.1 | 0.00083 |
- |
cyclosarin |
in 50 mM Tris-HCl buffer, 10 mM CaCl2, pH 7.4 |
708095 |
| 3.1.8.1 | 0.00094 |
- |
4-acetylphenyl cyclohexyl (S)-methylphosphonate |
mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C |
714216 |
| 3.1.8.1 | 0.0011 |
- |
4-acetylphenyl (2S)-3,3-dimethylbutan-2-yl (S)-methylphosphonate |
mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C |
714216 |
| 3.1.8.1 | 0.00186 |
- |
ethyl-paraoxon |
pH 8.0, 70°C |
730946 |
| 3.1.8.1 | 0.003 |
- |
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (R)-methylphosphonate |
mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C |
714216 |
| 3.1.8.1 | 0.0032 |
- |
4-acetylphenyl (2S)-3,3-dimethylbutan-2-yl (S)-methylphosphonate |
wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C |
714216 |
| 3.1.8.1 | 0.00486 |
- |
S-(2-[di(propan-2-yl)amino]ethyl)O-ethyl methylphosphonothioate |
expression mutant A80V/I106V/F132D/K185R/D208G/H257W/I274N/S308L/R319S, pH 8.5, temperature not specified in the publication |
729147 |
| 3.1.8.1 | 0.0056 |
- |
malathion |
25°C, pH 9.0 |
726484 |
| 3.1.8.1 | 0.0067 |
- |
4-acetylphenyl (2S)-3,3-dimethylbutan-2-yl (S)-methylphosphonate |
mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C |
714216 |
