EC Number |
Application |
Reference |
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3.2.1.91 | analysis |
polymerization-based assay for determining the potency of cellulolytic enzyme formulations on pretreated biomass substrates by monitoring the autofluorescence of cellulose. The one-pot method is label-free, rapid, highly sensitive, and requires only a single pipetting step. Using model enzyme formulations derived from Trichoderma reesei, Trichoderma longibrachiatum, Talaromyces emersonii and recombinant bacterial minicellulosomes from Clostridium thermocellum, enzyme performance based on differences in thermostability, cellulose-binding domain targeting, and endo/exoglucanase synergy can be differentiated |
735430 |
3.2.1.91 | biofuel production |
cellulose hydrolysis is an important step in the production of bioethanol from cellulosic biomass. Two key cellulase enzymes, celB from Caldicellulosiruptor saccharolyticus and beta-glucosidase, are covalently immobilised on polystyrene treated with plasma immersion ion implantation (PIII) which creates radicals that form covalent bonds. The immobilized enzymes are used to produce glucose from carboxymethyl cellulose (CMC), a solubilised form of cellulose. The highest activity of the immobilised celB on PIII treated surfaces was achieved when their immobilisation is carried out at a pH in the range 5-6.5. The immobilized celB on the PIII treated surface had the same activation energy as free celB showing substrate accessibility is not affected by the presence of the surface. The Vmax and Km values of immobilized celB are comparable to those of equal free celB concentrations. The areal density of immobilized celB on the PIII treated surface is estimated to be 0.0003 mg/cm2. The polystyrene surface with immobilized celB at 45°C can be reused over four times (23 hours each) with approximately 30% total activity loss. High ratios of beta-glucosidase to celB enhance the activity of immobilized celB for hydrolysis of carboxymethyl cellulose |
749281 |
3.2.1.91 | biotechnology |
fusion of the C-terminus of Caulobacter crescentus surface (S)-layer protein RsaA with the beta-1,4-glycanase Cex from the cellulolytic bacterium Cellulomonas fimi yielding a robust, catalytically active product, biocatalyst system evaluation |
663678 |
3.2.1.91 | degradation |
application of recombinant CBH II in hydrolysis of corn stover and rice straw pretreated with sodium hydroxide by improving the exo-exosynergism between CBH II and CBH I in Hypocrea jecorina. The yields 94.7% and 83.3% are achieved in the enzymatic hydrolysis of corn stover and rice straw pretreated by sodium hydroxide, respectively |
735742 |
3.2.1.91 | degradation |
combined use of isoforms CBH A and Cbh C on degradation of cotton. Conversion after 72 h is about 19 % by weight, with an almost fourfold increase in enzymatic hydrolysis yield by intermittent product removal of cellobiose with membrane filtration. A synergistic effect, achieving about 27 % substrate conversion, is obtained by addition of endo-1,4-beta-D-glucanase |
-, 747379 |
3.2.1.91 | degradation |
construction of a consolidated bioprocessing-enabling yeast by constitutive expression of genes Cbh1 from Aspergillus aculeatus, Cbh1 and Cbh2 from Hypocrea jecorina. Additionally, Hypocrea jecorina Eg2, Aspergillus aculeatus Bgl1 are integrated into the Saccharomyces cerevisiae chromosome. The resultant strains expressing uni-, bi-, and trifunctional cellulases, respectively, exhibit corresponding cellulase activities and both the activities and glucose producing activity ascends. Evaluation in acid- and alkali-pretreated corncob containing media with 5 FPU exogenous cellulase/g biomass loading shows that compared with the control strains, the engineered strains efficiently ferment pretreated corncob to ethanol |
737305 |
3.2.1.91 | degradation |
using enzymatic extract from Myceliophthora thermophila JCP 1-4 to saccharify sugarcane bagasse pretreated with microwaves and glycerol, glucose and xylose yields obtained are 15.6% and 35.13% (2.2 g/l and 1.95 g/l), respectively |
-, 742765 |
3.2.1.91 | detergent |
usage in paper-, detergent- and textile industry |
657365 |
3.2.1.91 | industry |
development of efficient degradation of cellulosic biomass using cellulolytic enzymes, e.g. Ex-4, of white rot fungi |
-, 696830 |
3.2.1.91 | more |
ability to amplify a key fungal cellobiohydrolase I gene involved in plant litter decomposition has the potential to unlock the identity and dynamics of the cellulolytic fungal community in situ |
695739 |