Application | Comment | Organism |
---|---|---|
biotechnology | fusion of the C-terminus of Caulobacter crescentus surface (S)-layer protein RsaA with the beta-1,4-glycanase Cex from the cellulolytic bacterium Cellulomonas fimi yielding a robust, catalytically active product, biocatalyst system evaluation | Cellulomonas fimi |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of the recombinant Cex-RsaA fusion mutant by fusion of the C-terminus of Caulobacter crescentus surface (S)-layer protein RsaA with the beta-1,4-glycanase Cex from the cellulolytic bacterium Cellulomonas fimi, the RsaA C-terminus causes a spontaneous unstructured aggregation of the recombinant protein | Cellulomonas fimi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.6 | - |
4-nitrophenyl beta-D-cellobioside | wild-type Cex | Cellulomonas fimi | |
1.1 | - |
4-nitrophenyl beta-D-cellobioside | recombinant Cex-RsaA fusion mutant | Cellulomonas fimi |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cellulose + H2O | Cellulomonas fimi | cellulolytic enzyme | cellobiose + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cellulomonas fimi | P07986 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-cellobioside + H2O | substrate of wild-type enzyme and recombinant Cex-RsaA fusion mutant | Cellulomonas fimi | 4-nitrophenol + beta-D-cellobiose | - |
? | |
cellulose + H2O | - |
Cellulomonas fimi | cellobiose + ? | - |
? | |
cellulose + H2O | cellulolytic enzyme | Cellulomonas fimi | cellobiose + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beta-1,4-glycanase | - |
Cellulomonas fimi |
Cex | - |
Cellulomonas fimi |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
4-nitrophenyl beta-D-cellobioside | recombinant Cex-RsaA fusion mutant | Cellulomonas fimi | |
15.8 | - |
4-nitrophenyl beta-D-cellobioside | wild-type Cex | Cellulomonas fimi |