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Literature summary for 3.2.1.91 extracted from

  • Duncan, G.; Tarling, C.A.; Bingle, W.H.; Nomellini, J.F.; Yamage, M.; Dorocicz, I.R.; Withers, S.G.; Smit, J.
    Evaluation of a new system for developing particulate enzymes based on the surface (S)-layer protein (RsaA) of Caulobacter crescentus: fusion with the beta-1,4-glycanase (Cex) from the cellulolytic bacterium Cellulomonas fimi yields a robust, catalytically active product (2005), Appl. Biochem. Biotechnol., 127, 95-110.
    View publication on PubMed

Application

Application Comment Organism
biotechnology fusion of the C-terminus of Caulobacter crescentus surface (S)-layer protein RsaA with the beta-1,4-glycanase Cex from the cellulolytic bacterium Cellulomonas fimi yielding a robust, catalytically active product, biocatalyst system evaluation Cellulomonas fimi

Protein Variants

Protein Variants Comment Organism
additional information construction of the recombinant Cex-RsaA fusion mutant by fusion of the C-terminus of Caulobacter crescentus surface (S)-layer protein RsaA with the beta-1,4-glycanase Cex from the cellulolytic bacterium Cellulomonas fimi, the RsaA C-terminus causes a spontaneous unstructured aggregation of the recombinant protein Cellulomonas fimi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.6
-
4-nitrophenyl beta-D-cellobioside wild-type Cex Cellulomonas fimi
1.1
-
4-nitrophenyl beta-D-cellobioside recombinant Cex-RsaA fusion mutant Cellulomonas fimi

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cellulose + H2O Cellulomonas fimi cellulolytic enzyme cellobiose + ?
-
?

Organism

Organism UniProt Comment Textmining
Cellulomonas fimi P07986
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-cellobioside + H2O substrate of wild-type enzyme and recombinant Cex-RsaA fusion mutant Cellulomonas fimi 4-nitrophenol + beta-D-cellobiose
-
?
cellulose + H2O
-
Cellulomonas fimi cellobiose + ?
-
?
cellulose + H2O cellulolytic enzyme Cellulomonas fimi cellobiose + ?
-
?

Synonyms

Synonyms Comment Organism
beta-1,4-glycanase
-
Cellulomonas fimi
Cex
-
Cellulomonas fimi

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.08
-
4-nitrophenyl beta-D-cellobioside recombinant Cex-RsaA fusion mutant Cellulomonas fimi
15.8
-
4-nitrophenyl beta-D-cellobioside wild-type Cex Cellulomonas fimi