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Literature summary for 3.2.1.91 extracted from
- Heterologous expression and production of Trichoderma reesei cellobiohydrolase II in Pichia pastoris and the application in the enzymatic hydrolysis of corn stover and rice straw (2015), Biomass Bioenergy, 78, 99-109.
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| degradation | application of recombinant CBH II in hydrolysis of corn stover and rice straw pretreated with sodium hydroxide by improving the exo-exosynergism between CBH II and CBH I in Hypocrea jecorina. The yields 94.7% and 83.3% are achieved in the enzymatic hydrolysis of corn stover and rice straw pretreated by sodium hydroxide, respectively | Trichoderma reesei |
| synthesis | expression in Pichia pastoris after codon optimization and using the strong methanol-inducible promoter AOX1. 5.84 U CBH II per ml can be obtained at 96 h | Trichoderma reesei |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Pichia pastoris | Trichoderma reesei |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Trichoderma reesei | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 58000 | - |
x * 58000, SDS-PAGE, recombinant protein | Trichoderma reesei |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trichoderma reesei | P07987 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| microcrystalline cellulose + H2O | - |
Trichoderma reesei | ? | - |
? |  |
| pretreated corn-stover + H2O | - |
Trichoderma reesei | ? | - |
? | |
| pretreated rice straw + H2O | - |
Trichoderma reesei | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 58000, SDS-PAGE, recombinant protein | Trichoderma reesei |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CBH II | - |
Trichoderma reesei |
| CBH2 | - |
Trichoderma reesei |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
recombinant protein | Trichoderma reesei |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
recombinant protein | Trichoderma reesei |
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Release 2023.1 (January 2023)
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