EC Number   |
Localization |
GeneOntology No. |
Reference |
|---|
    3.5.1.1 | cytoplasm |
- |
GO:0005737 AmiGO QuickGO |
-, 688355, 713442 |
| 3.5.1.1 | cytoplasm |
activity of L-asparaginase is found to be in the cytoplasm of Pectobacterium carotovorum grown on medium containing L-asparagine and glucose respectively |
GO:0005737 AmiGO QuickGO |
713442 |
| 3.5.1.1 | cytoplasm |
study of the subcelluar distribution of L-asparaginase in various strains |
GO:0005737 AmiGO QuickGO |
713442 |
| 3.5.1.1 | cytosol |
- |
GO:0005829 AmiGO QuickGO |
208957, 667061, 710917 |
| 3.5.1.1 | extracellular |
- |
- |
-, 208943, 208958, 665436, 686239, 752531, 753938 |
| 3.5.1.1 | intracellular |
- |
GO:0005622 AmiGO QuickGO |
686239 |
| 3.5.1.1 | intracellular |
localized external to the cytoplasmic membrane |
GO:0005622 AmiGO QuickGO |
-, 208951 |
| 3.5.1.1 | microsome |
- |
- |
686239 |
| 3.5.1.1 | periplasm |
- |
- |
-, 208963, 669734, 684128, 713442, 733839 |
| 3.5.1.1 | periplasm |
high-yield extraction of the periplasmic asparaginase produced by the recombinant Pichia pastoris strain habouring the Saccharomyes cerevisiae ASP3 gene. The use of six freeze-thaw cycles, folllowed by extraction with 20 mM potassium phosphate buffer pH 7.0 for 20 h, resulted in 85% enzyme recovery whereas the alkaline extraction using 500 mM potassium phosphate at pH 11.5 in the presence of 10 mM cysteine allows 100% enzyme recovery |
- |
711873 |
