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Literature summary for 3.5.1.1 extracted from

  • Kumar, S.; Dasu, V.; Pakshirajan, K.
    Localization and production of novel L-asparaginase from Pectobacterium carotovorum MTCC 1428 (2010), Process Biochem., 45, 223-229.
No PubMed abstract available
Search for more literature for 3.5.1.1

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Serratia marcescens 5737
-
cytoplasm activity of L-asparaginase is found to be in the cytoplasm of Pectobacterium carotovorum grown on medium containing L-asparagine and glucose respectively Pectobacterium carotovorum 5737
-
cytoplasm study of the subcelluar distribution of L-asparaginase in various strains Cupriavidus necator 5737
-
periplasm
-
 Serratia marcescens
-
-
periplasm
-
 Pectobacterium carotovorum
-
-
periplasm study of the subcelluar distribution of L-asparaginase in various strains Cupriavidus necator
-
-

Organism

Organism UniProt Comment Textmining
Cupriavidus necator
-
 NRRL B-2804
-
Pectobacterium carotovorum
-
 MTCC 1428
-
Serratia marcescens
-
-
-
Serratia marcescens MTCC 97
-
-
-
Serratia marcescens NCIM 2919
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:D-glucose-grown cell study of the effect of various carbon sources on pH of the broth and L-asparaginase production Serratia marcescens
-
culture condition:D-glucose-grown cell study of the effect of various carbon sources on pH of the broth and L-asparaginase production Cupriavidus necator
-
culture condition:D-glucose-grown cell study of the effect of various carbon sources on pH of the broth and L-asparaginase production Pectobacterium carotovorum
-
culture condition:D-glucose-L-asparagine-grown cell study of the effect of various carbon sources on pH of the broth and L-asparaginase production Serratia marcescens
-
culture condition:D-glucose-L-asparagine-grown cell study of the effect of various carbon sources on pH of the broth and L-asparaginase production Cupriavidus necator
-
culture condition:D-glucose-L-asparagine-grown cell study of the effect of various carbon sources on pH of the broth and L-asparaginase production Pectobacterium carotovorum
-
culture condition:L-asparagine-grown cell study of the effect of various carbon sources on pH of the broth and L-asparaginase production Serratia marcescens
-
culture condition:L-asparagine-grown cell study of the effect of various carbon sources on pH of the broth and L-asparaginase production Cupriavidus necator
-
culture condition:L-asparagine-grown cell study of the effect of various carbon sources on pH of the broth and L-asparaginase production Pectobacterium carotovorum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.65
-
 Pectobacterium carotovorum grown on medium containing L-asparagine, localized in the periplasm, 37°C, pH 8.6 Pectobacterium carotovorum
1.99
-
 Wautersia eutropha grown on medium containing L-asparagine, localized in the periplasm, 37°C, pH 8.6 Cupriavidus necator
2.11
-
 Serratia marcescens NCIM 2919 grown on medium containing L-asparagine, localized in the periplasm, 37°C, pH 8.6 Serratia marcescens
2.52
-
 Serratia marcescens MTCC 97 grown on medium containing glucose + L-asparagine, localized in the periplasm, 37°C, pH 8.6 Serratia marcescens
2.94
-
 Serratia marcescens MTCC 97 grown on medium containing glucose, localized in the periplasm, 37°C, pH 8.6 Serratia marcescens
2.99
-
 Serratia marcescens MTCC 97 grown on medium containing L-asparagine, localized in the periplasm, 37°C, pH 8.6 Serratia marcescens
3.35
-
 Pectobacterium carotovorum grown on medium containing glucose, localized in the cytoplasm, 37°C, pH 8.6 Pectobacterium carotovorum
3.61
-
 Pectobacterium carotovorum grown on medium containing glucose + L-asparagine, localized in the periplasm, 37°C, pH 8.6 Pectobacterium carotovorum
3.63
-
 Wautersia eutropha grown on medium containing glucose + L-asparagine, localized in the periplasm, 37°C, pH 8.6 Cupriavidus necator
3.64
-
 Serratia marcescens NCIM 2919 grown on medium containing glucose + L-asparagine, localized in the periplasm, 37°C, pH 8.6 Serratia marcescens
3.81
-
 Serratia marcescens NCIM 2919 grown on medium containing glucose, localized in the periplasm, 37°C, pH 8.6 Serratia marcescens
4.3
-
 Pectobacterium carotovorum grown on medium containing glucose, localized in the periplasm, 37°C, pH 8.6 Pectobacterium carotovorum
5.41
-
 Wautersia eutropha grown on medium containing glucose, localized in the periplasm, 37°C, pH 8.6 Cupriavidus necator
7.25
-
 Serratia marcescens NCIM 2919 grown on medium containing glucose, localized in the cytoplasm, 37°C, pH 8.6 Serratia marcescens
7.48
-
 Wautersia eutropha grown on medium containing glucose, localized in the cytoplasm, 37°C, pH 8.6 Cupriavidus necator
8.36
-
 Serratia marcescens MTCC 97 grown on medium containing glucose, localized in the cytoplasm, 37°C, pH 8.6 Serratia marcescens
15.36
-
 Wautersia eutropha grown on medium containing L-asparagine, localized in the cytoplasm, 37°C, pH 8.6 Cupriavidus necator
18.29
-
 Wautersia eutropha grown on medium containing glucose + L-asparagine, localized in the cytoplasm, 37°C, pH 8.6 Cupriavidus necator
21.6
-
 Pectobacterium carotovorum grown on medium containing glucose + L-asparagine, localized in the cytoplasm, 37°C, pH 8.6 Pectobacterium carotovorum
22.45
-
 Pectobacterium carotovorum grown on medium containing L-asparagine, localized in the cytoplasm, 37°C, pH 8.6 Pectobacterium carotovorum
28.13
-
 Serratia marcescens MTCC 97 grown on medium containing L-asparagine, localized in the cytoplasm, 37°C, pH 8.6 Serratia marcescens
30.57
-
 Serratia marcescens MTCC 97 grown on medium containing glucose + L-asparagine, localized in the cytoplasm, 37°C, pH 8.6 Serratia marcescens
31.45
-
 Serratia marcescens NCIM 2919 grown on medium containing glucose + L-asparagine, localized in the cytoplasm, 37°C, pH 8.6 Serratia marcescens
33.73
-
 Serratia marcescens NCIM 2919 grown on medium containing L-asparagine, localized in the cytoplasm, 37°C, pH 8.6 Serratia marcescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-asparagine + H2O
-
 Serratia marcescens L-aspartate + NH3
-
 ?
L-asparagine + H2O
-
 Cupriavidus necator L-aspartate + NH3
-
 ?
L-asparagine + H2O
-
 Pectobacterium carotovorum L-aspartate + NH3
-
 ?
L-asparagine + H2O
-
 Serratia marcescens NCIM 2919 L-aspartate + NH3
-
 ?
L-asparagine + H2O
-
 Serratia marcescens MTCC 97 L-aspartate + NH3
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Pectobacterium carotovorum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.6
-
 assay at Pectobacterium carotovorum